BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146651.13 - phase: 0
(274 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC15910 similar to UP|Q9AR64 (Q9AR64) Urease accessory protein G... 506 e-144
BP047504 59 9e-10
AV766647 41 2e-04
TC15331 similar to GB|AAM16226.1|20334730|AY093965 At1g15730/F7H... 39 0.001
TC10944 37 0.003
BG662335 35 0.011
TC9068 similar to UP|SCWA_YEAST (Q04951) Probable family 17 gluc... 35 0.018
BP080689 34 0.031
TC14758 similar to UP|O24601 (O24601) Glycine-rich RNA binding p... 34 0.031
TC11490 similar to UP|O48566 (O48566) CLP protease regulatory su... 33 0.040
BP040113 32 0.089
TC13343 similar to UP|Q9FMR9 (Q9FMR9) Ruv DNA-helicase-like prot... 32 0.089
AW164001 32 0.12
TC15376 weakly similar to UP|Q7SYH2 (Q7SYH2) Cystatin domain fet... 32 0.15
TC11976 similar to GB|AAP12856.1|30017245|BT006207 At3g02340 {Ar... 32 0.15
TC8582 similar to UP|Q8LJS2 (Q8LJS2) Nucleolar histone deacetyla... 31 0.20
TC8330 weakly similar to GB|AAM61257.1|21536925|AY084696 calmodu... 31 0.26
TC14791 similar to UP|Q9LJ04 (Q9LJ04) ESTs AU082210(C53655), par... 31 0.26
AV773558 30 0.34
TC17661 weakly similar to UP|Q09085 (Q09085) Hydroxyproline-rich... 30 0.34
>TC15910 similar to UP|Q9AR64 (Q9AR64) Urease accessory protein G, partial
(88%)
Length = 1061
Score = 506 bits (1304), Expect = e-144
Identities = 248/290 (85%), Positives = 258/290 (88%), Gaps = 17/290 (5%)
Frame = +2
Query: 2 ASGGDHTHVHDHDHHHHDHDHKH-----------------GDSFIGADGKVYHSHDGLAP 44
AS GDH H H H HHH DH H H DS++G DGKVYHSHDGLAP
Sbjct: 2 ASAGDHNHEHHHHHHHSDHGHDHDHHDNSHSHSDGVASAGSDSYVGPDGKVYHSHDGLAP 181
Query: 45 HSHEPIYSPGFFSRRAQPLINRDFNERAFTVGIGGPVGTGKTALMLALCQNLRDRYSLAA 104
HSHEPIYSPGFF+RRA PLINRDFNERAFT+GIGGPVGTGKTALMLALC+ LRD YSLAA
Sbjct: 182 HSHEPIYSPGFFTRRAPPLINRDFNERAFTIGIGGPVGTGKTALMLALCEFLRDNYSLAA 361
Query: 105 VTNDIFTKEDGEFLVKHKALPEERIRAVETGGCPHAAIREDISINLGPLEELSNLYKADL 164
VTNDIFTKEDGEFLV++KALPEERIRAVETGGCPHAAIREDISINLGPLEELSNLYK D+
Sbjct: 362 VTNDIFTKEDGEFLVRNKALPEERIRAVETGGCPHAAIREDISINLGPLEELSNLYKTDI 541
Query: 165 LLCESGGDNLAANFSRELADYIIYIIDVSGGDKIPRKGGPGITQADLLVINKTDLAQAIG 224
LLCESGGDNLAANFSRELADYIIYIIDVSGGDKIPRKGGPGITQADLLVINKTDLAQAIG
Sbjct: 542 LLCESGGDNLAANFSRELADYIIYIIDVSGGDKIPRKGGPGITQADLLVINKTDLAQAIG 721
Query: 225 ADLGVMERDALRMRDGGPFVFAQVKHNVGVEEIVNHVLQAWEAATGKKRH 274
ADLGVMERD+LRMRDGGPFVFAQVKH VGVEEIVNHV+QAWEAATGKKRH
Sbjct: 722 ADLGVMERDSLRMRDGGPFVFAQVKHKVGVEEIVNHVIQAWEAATGKKRH 871
>BP047504
Length = 486
Score = 58.9 bits (141), Expect = 9e-10
Identities = 26/28 (92%), Positives = 27/28 (95%)
Frame = -1
Query: 247 QVKHNVGVEEIVNHVLQAWEAATGKKRH 274
QVKH VGVEEIVNHV+QAWEAATGKKRH
Sbjct: 324 QVKHKVGVEEIVNHVIQAWEAATGKKRH 241
>AV766647
Length = 349
Score = 41.2 bits (95), Expect = 2e-04
Identities = 14/28 (50%), Positives = 15/28 (53%)
Frame = -3
Query: 7 HTHVHDHDHHHHDHDHKHGDSFIGADGK 34
H H H H HHHH H H+HG D K
Sbjct: 341 HQHPHPHHHHHHHHHHEHGGGCCSGDAK 258
>TC15331 similar to GB|AAM16226.1|20334730|AY093965 At1g15730/F7H2_7
{Arabidopsis thaliana;}, partial (25%)
Length = 657
Score = 38.9 bits (89), Expect = 0.001
Identities = 19/43 (44%), Positives = 22/43 (50%), Gaps = 4/43 (9%)
Frame = +1
Query: 11 HDHDHHHH----DHDHKHGDSFIGADGKVYHSHDGLAPHSHEP 49
HDH HHHH DH H H D +HSHD H+H+P
Sbjct: 1 HDHHHHHHEDSLDHKHDHHD---------HHSHD----HTHDP 90
Score = 34.7 bits (78), Expect = 0.018
Identities = 16/45 (35%), Positives = 20/45 (43%)
Frame = +1
Query: 13 HDHHHHDHDHKHGDSFIGADGKVYHSHDGLAPHSHEPIYSPGFFS 57
HDHHHH H+ + H HD HSH+ + PG S
Sbjct: 1 HDHHHHHHEDS-----------LDHKHDHHDHHSHDHTHDPGVSS 102
Score = 34.3 bits (77), Expect = 0.023
Identities = 14/24 (58%), Positives = 15/24 (62%)
Frame = +1
Query: 6 DHTHVHDHDHHHHDHDHKHGDSFI 29
DH H H HDHH HDH H G S +
Sbjct: 37 DHKHDH-HDHHSHDHTHDPGVSSV 105
>TC10944
Length = 512
Score = 37.4 bits (85), Expect = 0.003
Identities = 18/45 (40%), Positives = 25/45 (55%), Gaps = 4/45 (8%)
Frame = +1
Query: 2 ASGGDHTHVHDHD----HHHHDHDHKHGDSFIGADGKVYHSHDGL 42
++G DH H+H H + HH H HG ++ A G + HSH GL
Sbjct: 67 SNGHDHDHLHHHSHGHGYDHHGDAHDHGAGYMDAYG-LGHSHHGL 198
Score = 32.0 bits (71), Expect = 0.12
Identities = 13/30 (43%), Positives = 15/30 (49%)
Frame = -2
Query: 11 HDHDHHHHDHDHKHGDSFIGADGKVYHSHD 40
H +HHH DH H H DG+ H HD
Sbjct: 148 HGREHHHGDHTHDH-----DCDGEDDHDHD 74
>BG662335
Length = 336
Score = 35.4 bits (80), Expect = 0.011
Identities = 11/18 (61%), Positives = 11/18 (61%)
Frame = +1
Query: 7 HTHVHDHDHHHHDHDHKH 24
H H H H HHHH H H H
Sbjct: 283 HQHQHPHPHHHHHHHHHH 336
Score = 30.8 bits (68), Expect = 0.26
Identities = 10/20 (50%), Positives = 10/20 (50%)
Frame = +1
Query: 5 GDHTHVHDHDHHHHDHDHKH 24
G H H H H HH H H H
Sbjct: 271 GKQLHQHQHPHPHHHHHHHH 330
>TC9068 similar to UP|SCWA_YEAST (Q04951) Probable family 17 glucosidase
SCW10 precursor (Soluble cell wall protein 10) ,
partial (6%)
Length = 1209
Score = 34.7 bits (78), Expect = 0.018
Identities = 11/18 (61%), Positives = 11/18 (61%)
Frame = -2
Query: 7 HTHVHDHDHHHHDHDHKH 24
H H H H HHHH H H H
Sbjct: 146 HHHHHHHHHHHHHHHHHH 93
Score = 34.7 bits (78), Expect = 0.018
Identities = 11/18 (61%), Positives = 11/18 (61%)
Frame = -2
Query: 7 HTHVHDHDHHHHDHDHKH 24
H H H H HHHH H H H
Sbjct: 149 HHHHHHHHHHHHHHHHHH 96
Score = 34.7 bits (78), Expect = 0.018
Identities = 11/18 (61%), Positives = 11/18 (61%)
Frame = -2
Query: 7 HTHVHDHDHHHHDHDHKH 24
H H H H HHHH H H H
Sbjct: 140 HHHHHHHHHHHHHHHHHH 87
Score = 34.7 bits (78), Expect = 0.018
Identities = 11/18 (61%), Positives = 11/18 (61%)
Frame = -2
Query: 7 HTHVHDHDHHHHDHDHKH 24
H H H H HHHH H H H
Sbjct: 137 HHHHHHHHHHHHHHHHHH 84
Score = 34.7 bits (78), Expect = 0.018
Identities = 11/18 (61%), Positives = 11/18 (61%)
Frame = -2
Query: 7 HTHVHDHDHHHHDHDHKH 24
H H H H HHHH H H H
Sbjct: 143 HHHHHHHHHHHHHHHHHH 90
Score = 33.5 bits (75), Expect = 0.040
Identities = 11/20 (55%), Positives = 12/20 (60%)
Frame = -2
Query: 7 HTHVHDHDHHHHDHDHKHGD 26
H H H H HHHH H H+ D
Sbjct: 131 HHHHHHHHHHHHHHHHRAWD 72
>BP080689
Length = 326
Score = 33.9 bits (76), Expect = 0.031
Identities = 11/18 (61%), Positives = 12/18 (66%)
Frame = +1
Query: 13 HDHHHHDHDHKHGDSFIG 30
H HHHH H H H +FIG
Sbjct: 4 HHHHHHHHHHHHAHNFIG 57
Score = 29.3 bits (64), Expect = 0.76
Identities = 9/15 (60%), Positives = 10/15 (66%)
Frame = +1
Query: 7 HTHVHDHDHHHHDHD 21
H H H H HHHH H+
Sbjct: 4 HHHHHHHHHHHHAHN 48
>TC14758 similar to UP|O24601 (O24601) Glycine-rich RNA binding protein 2,
partial (93%)
Length = 725
Score = 33.9 bits (76), Expect = 0.031
Identities = 15/41 (36%), Positives = 17/41 (40%)
Frame = -2
Query: 7 HTHVHDHDHHHHDHDHKHGDSFIGADGKVYHSHDGLAPHSH 47
H H HHHH H H H D +H+H L H H
Sbjct: 532 HRRCHSGSHHHHHHIHLHDD---------HHNHRRLLLHIH 437
Score = 26.2 bits (56), Expect = 6.4
Identities = 13/29 (44%), Positives = 15/29 (50%), Gaps = 7/29 (24%)
Frame = -2
Query: 3 SGGDHTHVHDH---DHHHHD----HDHKH 24
SG H H H H DHH+H H H+H
Sbjct: 517 SGSHHHHHHIHLHDDHHNHRRLLLHIHRH 431
>TC11490 similar to UP|O48566 (O48566) CLP protease regulatory subunit CLPX
precursor, partial (4%)
Length = 528
Score = 33.5 bits (75), Expect = 0.040
Identities = 14/42 (33%), Positives = 20/42 (47%), Gaps = 5/42 (11%)
Frame = -3
Query: 5 GDHTHVHDHDHHHHDHD-----HKHGDSFIGADGKVYHSHDG 41
G + H HHHH H H+H S++G + + Y S G
Sbjct: 394 GPEEYAQPHHHHHHHHHRLEGFHRHPTSYLGREVQCYSSCSG 269
>BP040113
Length = 439
Score = 32.3 bits (72), Expect = 0.089
Identities = 15/38 (39%), Positives = 23/38 (60%), Gaps = 1/38 (2%)
Frame = +2
Query: 77 IGGPVGTGKTALMLALCQNLRDRYSLA-AVTNDIFTKE 113
+ GP GTGKTAL L +CQ L + V +++++ E
Sbjct: 293 LAGPPGTGKTALALGICQELGTKVPFCPMVGSEVYSSE 406
>TC13343 similar to UP|Q9FMR9 (Q9FMR9) Ruv DNA-helicase-like protein,
partial (21%)
Length = 518
Score = 32.3 bits (72), Expect = 0.089
Identities = 15/38 (39%), Positives = 23/38 (60%), Gaps = 1/38 (2%)
Frame = +1
Query: 77 IGGPVGTGKTALMLALCQNLRDRYSLA-AVTNDIFTKE 113
+ GP GTGKTAL L +CQ L + V +++++ E
Sbjct: 373 LAGPPGTGKTALALGICQELGTKVPFCPMVGSEVYSSE 486
>AW164001
Length = 447
Score = 32.0 bits (71), Expect = 0.12
Identities = 10/18 (55%), Positives = 11/18 (60%)
Frame = -3
Query: 7 HTHVHDHDHHHHDHDHKH 24
H H H H HHHH + H H
Sbjct: 127 HHHHHHHHHHHHQNPHLH 74
Score = 28.9 bits (63), Expect = 0.99
Identities = 9/15 (60%), Positives = 9/15 (60%)
Frame = -3
Query: 10 VHDHDHHHHDHDHKH 24
V H HHHH H H H
Sbjct: 136 VRHHHHHHHHHHHHH 92
Score = 28.1 bits (61), Expect = 1.7
Identities = 10/18 (55%), Positives = 11/18 (60%)
Frame = -3
Query: 7 HTHVHDHDHHHHDHDHKH 24
H H H H HHHH H + H
Sbjct: 130 HHHHHHH-HHHHHHQNPH 80
>TC15376 weakly similar to UP|Q7SYH2 (Q7SYH2) Cystatin domain fetuin-like
protein, partial (6%)
Length = 582
Score = 31.6 bits (70), Expect = 0.15
Identities = 10/18 (55%), Positives = 11/18 (60%)
Frame = +1
Query: 7 HTHVHDHDHHHHDHDHKH 24
H H H H HH H H H+H
Sbjct: 385 HHHRHYHHHHRHRHHHRH 438
Score = 29.3 bits (64), Expect = 0.76
Identities = 9/18 (50%), Positives = 9/18 (50%)
Frame = +1
Query: 7 HTHVHDHDHHHHDHDHKH 24
H H H H H H H H H
Sbjct: 391 HRHYHHHHRHRHHHRHHH 444
Score = 28.1 bits (61), Expect = 1.7
Identities = 9/18 (50%), Positives = 9/18 (50%)
Frame = +1
Query: 7 HTHVHDHDHHHHDHDHKH 24
H H H HHH H H H
Sbjct: 397 HYHHHHRHRHHHRHHHHH 450
>TC11976 similar to GB|AAP12856.1|30017245|BT006207 At3g02340 {Arabidopsis
thaliana;}, partial (11%)
Length = 530
Score = 31.6 bits (70), Expect = 0.15
Identities = 13/36 (36%), Positives = 15/36 (41%)
Frame = -3
Query: 11 HDHDHHHHDHDHKHGDSFIGADGKVYHSHDGLAPHS 46
H H HHHH H H G I ++ H HS
Sbjct: 219 HHHHHHHHHHHHFTGSVQISEPSYAHNQHHQ*ETHS 112
Score = 28.1 bits (61), Expect = 1.7
Identities = 9/17 (52%), Positives = 10/17 (57%)
Frame = -3
Query: 15 HHHHDHDHKHGDSFIGA 31
HHHH H H H F G+
Sbjct: 222 HHHHHHHHHHHHHFTGS 172
>TC8582 similar to UP|Q8LJS2 (Q8LJS2) Nucleolar histone deacetylase
HD2-P39, partial (65%)
Length = 1341
Score = 31.2 bits (69), Expect = 0.20
Identities = 9/16 (56%), Positives = 10/16 (62%)
Frame = -3
Query: 7 HTHVHDHDHHHHDHDH 22
H H H H HHH+ H H
Sbjct: 652 HHHFHHHSHHHYPHQH 605
Score = 27.3 bits (59), Expect = 2.9
Identities = 9/20 (45%), Positives = 11/20 (55%)
Frame = -3
Query: 5 GDHTHVHDHDHHHHDHDHKH 24
G H H H H HH + H+H
Sbjct: 664 GSLPHHHFHHHSHHHYPHQH 605
>TC8330 weakly similar to GB|AAM61257.1|21536925|AY084696 calmodulin-like
protein {Arabidopsis thaliana;} , partial (72%)
Length = 1174
Score = 30.8 bits (68), Expect = 0.26
Identities = 11/33 (33%), Positives = 21/33 (63%)
Frame = -3
Query: 8 THVHDHDHHHHDHDHKHGDSFIGADGKVYHSHD 40
+H++ ++H+HDHDH S I ++H+H+
Sbjct: 815 SHIYIWNYHYHDHDHHMASSSI-----IHHNHN 732
>TC14791 similar to UP|Q9LJ04 (Q9LJ04) ESTs AU082210(C53655), partial (37%)
Length = 1779
Score = 30.8 bits (68), Expect = 0.26
Identities = 23/92 (25%), Positives = 34/92 (36%), Gaps = 11/92 (11%)
Frame = +2
Query: 3 SGGDHTHVHDHDHHHHDHDHK-----HGDSFIGADGKVYHSHDGLAPHSHEP---IYSPG 54
S G H + H H H HH H H+ H + G H G P Y+PG
Sbjct: 1142 SKGYHQNQHQHQHQHHSHHHQPHYQPHKKHKYNSGGGPSHGGGGHLMAPSNPGMGGYNPG 1321
Query: 55 FFSRRAQPLINRD---FNERAFTVGIGGPVGT 83
+ P + + + +G+G +GT
Sbjct: 1322 VQGQGLNPALGQALALLANQGAGLGLGNLLGT 1417
Score = 25.8 bits (55), Expect = 8.4
Identities = 11/38 (28%), Positives = 14/38 (35%)
Frame = +2
Query: 4 GGDHTHVHDHDHHHHDHDHKHGDSFIGADGKVYHSHDG 41
G H + H H H H H H + Y+S G
Sbjct: 1139 GSKGYHQNQHQHQHQHHSHHHQPHYQPHKKHKYNSGGG 1252
>AV773558
Length = 469
Score = 30.4 bits (67), Expect = 0.34
Identities = 9/14 (64%), Positives = 9/14 (64%)
Frame = +3
Query: 11 HDHDHHHHDHDHKH 24
H H HHHH H H H
Sbjct: 327 HHHHHHHHHHHHPH 368
Score = 30.0 bits (66), Expect = 0.44
Identities = 9/16 (56%), Positives = 10/16 (62%)
Frame = +3
Query: 9 HVHDHDHHHHDHDHKH 24
H+ H HHHH H H H
Sbjct: 312 HLCSHHHHHHHHHHHH 359
Score = 29.6 bits (65), Expect = 0.58
Identities = 9/14 (64%), Positives = 9/14 (64%)
Frame = +3
Query: 9 HVHDHDHHHHDHDH 22
H H H HHHH H H
Sbjct: 327 HHHHHHHHHHHHPH 368
Score = 29.3 bits (64), Expect = 0.76
Identities = 9/14 (64%), Positives = 9/14 (64%)
Frame = +3
Query: 7 HTHVHDHDHHHHDH 20
H H H H HHHH H
Sbjct: 327 HHHHHHHHHHHHPH 368
Score = 28.5 bits (62), Expect = 1.3
Identities = 9/16 (56%), Positives = 9/16 (56%)
Frame = +3
Query: 7 HTHVHDHDHHHHDHDH 22
H H H HHHH H H
Sbjct: 312 HLCSHHHHHHHHHHHH 359
Score = 26.6 bits (57), Expect = 4.9
Identities = 9/18 (50%), Positives = 9/18 (50%)
Frame = +3
Query: 7 HTHVHDHDHHHHDHDHKH 24
H H HHHH H H H
Sbjct: 303 HPHHLCSHHHHHHHHHHH 356
>TC17661 weakly similar to UP|Q09085 (Q09085) Hydroxyproline-rich
glycoprotein (HRGP) (Fragment), partial (8%)
Length = 417
Score = 30.4 bits (67), Expect = 0.34
Identities = 14/48 (29%), Positives = 22/48 (45%)
Frame = +2
Query: 3 SGGDHTHVHDHDHHHHDHDHKHGDSFIGADGKVYHSHDGLAPHSHEPI 50
SGG ++H HHHH H H + + ++H+H +H I
Sbjct: 2 SGGGTATANNHRHHHHHHHHAILFTHLHPLQLLHHTHMDHRESTHSTI 145
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.319 0.139 0.420
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,006,699
Number of Sequences: 28460
Number of extensions: 87324
Number of successful extensions: 2408
Number of sequences better than 10.0: 157
Number of HSP's better than 10.0 without gapping: 1080
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1821
length of query: 274
length of database: 4,897,600
effective HSP length: 89
effective length of query: 185
effective length of database: 2,364,660
effective search space: 437462100
effective search space used: 437462100
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)
Medicago: description of AC146651.13