BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146632.7 + phase: 0
(339 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BI418251 91 4e-19
TC12546 weakly similar to UP|Q9FJ30 (Q9FJ30) Similarity to heat ... 79 1e-15
CB829128 70 4e-13
TC15781 64 5e-11
AU088858 60 4e-10
AV780048 57 3e-09
AV428270 52 2e-07
TC11299 52 2e-07
AU251749 33 2e-05
BP063614 41 3e-04
BI420296 40 7e-04
BP071245 40 7e-04
AV419392 37 0.005
AV423751 36 0.011
BP050969 35 0.018
TC17934 similar to PIR|T06667|T06667 argininosuccinate synthase ... 35 0.018
TC14800 weakly similar to UP|Q9FHP3 (Q9FHP3) Genomic DNA, chromo... 34 0.040
TC14802 weakly similar to UP|CAD56661 (CAD56661) S locus F-box (... 34 0.040
BG662160 33 0.052
AW720409 33 0.068
>BI418251
Length = 546
Score = 90.5 bits (223), Expect = 4e-19
Identities = 60/178 (33%), Positives = 97/178 (53%), Gaps = 6/178 (3%)
Frame = +1
Query: 39 PNADRISILPDELLCRILSLLPAKQIMVTSLLSRRWRSLRPRMTEINIDDTSYIHDR--D 96
P ADRIS LPDE+LC+ILS LP + + TS+L +RW SL + ++ DD Y+ +
Sbjct: 28 PMADRISKLPDEVLCQILSFLPTEDAVATSVLCKRWSSLWLSVPTLDFDDYRYLKGKKLK 207
Query: 97 AYDRYYHVIALFLFEMKIHHPIMK-TVTILSASPFFNPLPLWLGCL---QVQHLDVTSSA 152
+ + + + +H PI T++++S + + +WL QV++LD+ S
Sbjct: 208 LQSSFINFVYAIILSRALHQPIKNFTLSVISEECPYPDVKVWLNAAMQRQVENLDICLSL 387
Query: 153 TLCLCVPYKVLNSTALVVLKLNALTIDYVHRSSTNLPSLKILHLTQVHFLKLKFLIKI 210
+ +P +L+ T LVVLKL+ + + S +LPSLK LHL V L + L+ +
Sbjct: 388 S---TLPCSILSCTTLVVLKLS--DVKFHVFSCVDLPSLKTLHLELVVILNPQSLMDL 546
>TC12546 weakly similar to UP|Q9FJ30 (Q9FJ30) Similarity to heat shock
transcription factor HSF30, partial (9%)
Length = 755
Score = 78.6 bits (192), Expect = 1e-15
Identities = 65/204 (31%), Positives = 100/204 (48%), Gaps = 21/204 (10%)
Frame = +2
Query: 38 MPNADRISILPDELLCRILSLLPAKQIMVTSLLSRRWRSLRPRMTEINIDDTSYIHD--- 94
M D IS LPD LLC ILS L +K+ + TS+LSRRW L + ++ D +Y D
Sbjct: 137 MKMEDGISTLPDALLCHILSFLTSKEAVATSVLSRRWIPLWRSVPTLHFKDANYHTDIGH 316
Query: 95 ------RDAYDRYYHVIALFLFEMKIHHPIMKTVTILS--ASPFFNP--LPLWLGCL--- 141
+D R+ + + PI K L+ PF++P + +W+ +
Sbjct: 317 ADHDIVKDVRSRHVQSVYAVILSRDFQLPIKKFYLRLNDVCQPFYDPANVSVWVNAVVQR 496
Query: 142 QVQHLDVTSSATLCLCVPYKVLNS----TALVVLKLNALTIDYVHRSSTNLPSLKILHLT 197
Q++HLD++ + L P L+S LVVLKL ++ S + P LK+LHL
Sbjct: 497 QLEHLDISLPYPM-LSTPRANLSSIFSCRTLVVLKLRG-GLELKRFPSVHFPCLKVLHLQ 670
Query: 198 QVHFL-KLKFLIKILSMSPLLEDL 220
L + +L ++LS P+LE+L
Sbjct: 671 GALLLHDVPYLAELLSGCPVLENL 742
>CB829128
Length = 463
Score = 70.5 bits (171), Expect = 4e-13
Identities = 52/143 (36%), Positives = 80/143 (55%), Gaps = 3/143 (2%)
Frame = +3
Query: 134 LPLWLGCL---QVQHLDVTSSATLCLCVPYKVLNSTALVVLKLNALTIDYVHRSSTNLPS 190
L +WL QV++L++ + + +P +L T LV+LKL+++T D +S LPS
Sbjct: 24 LKVWLNAAIQRQVENLEIELTDSQ---MPCSILRCTTLVILKLDSVTFDAF--TSVYLPS 188
Query: 191 LKILHLTQVHFLKLKFLIKILSMSPLLEDLLLKDLQVTDNTLAHDDAAALKPFPKLLRAD 250
LK LHL +V +K + LI +L P+LEDL + + D + +K KL+RAD
Sbjct: 189 LKTLHLFEVDLVKAQCLIDLLYGCPILEDLKTQ-IYFRDGSF----EGKVKTLSKLVRAD 353
Query: 251 VSESSISAFLLPLKLFYNVQFLR 273
V F++P+K F NV+FLR
Sbjct: 354 VFLD--GHFIIPVKAFQNVEFLR 416
>TC15781
Length = 621
Score = 63.5 bits (153), Expect = 5e-11
Identities = 43/143 (30%), Positives = 68/143 (47%), Gaps = 7/143 (4%)
Frame = +2
Query: 137 WLGCLQVQHLDVTSSATLCLCVP-------YKVLNSTALVVLKLNALTIDYVHRSSTNLP 189
WL + + ++ L +C+P K+ + LVVLKL + S LP
Sbjct: 131 WLNTVMPRRIE-----NLEICIPPLSRGIGLKIYSCRTLVVLKLKGYPLGVYVSSHVELP 295
Query: 190 SLKILHLTQVHFLKLKFLIKILSMSPLLEDLLLKDLQVTDNTLAHDDAAALKPFPKLLRA 249
SLK LHL +VHF+ + +K++ PLLEDL + D + D + PKL+
Sbjct: 296 SLKSLHLEKVHFVDFQSFLKLICGCPLLEDLEAGTVSYLDTSF--DQEFRFRSLPKLVSV 469
Query: 250 DVSESSISAFLLPLKLFYNVQFL 272
+++ + F PLK+ N +FL
Sbjct: 470 VINDYN---FRFPLKIISNAEFL 529
>AU088858
Length = 765
Score = 60.5 bits (145), Expect = 4e-10
Identities = 44/142 (30%), Positives = 73/142 (50%), Gaps = 1/142 (0%)
Frame = +3
Query: 184 SSTNLPSLKILHLTQVHFLKLKFLIKILSMSPLLEDLLLKDLQVTDNTLAHDDAAALKPF 243
S +LPSLK L L + F + ++++++LS P+LE + L ++ D + +K
Sbjct: 9 SFVHLPSLKTLQLYCLIFPEPQYVMELLSGCPILEYMHLSRMRYAD--CSSPSNTNVKSL 182
Query: 244 PKLLRADVSESSISAFLLPLKLFYNVQFLRSQVLLQTLEQDFSTTQFLNLTHMDLIFHD- 302
KL+ AD+ S F +PLK+ NV+FLR + + F NLTH++L+
Sbjct: 183 TKLVSADI---SFIGFEIPLKVICNVEFLRID------KYPYEIPVFPNLTHLELMMSGI 335
Query: 303 GYYWISLMKFICACPSLQTLTI 324
G W ++ + CP LQ L +
Sbjct: 336 GMNWHLVLAMLKKCPKLQKLLL 401
>AV780048
Length = 501
Score = 57.4 bits (137), Expect = 3e-09
Identities = 29/83 (34%), Positives = 44/83 (52%)
Frame = +2
Query: 38 MPNADRISILPDELLCRILSLLPAKQIMVTSLLSRRWRSLRPRMTEINIDDTSYIHDRDA 97
M DR S LPD +LC ILSLL K+ + TS+LS+RW L + ++ DD+++ +
Sbjct: 239 MKMEDRFSSLPDPILCHILSLLTTKEAVTTSILSKRWIPLWRSVPTLDFDDSNWYGGDEV 418
Query: 98 YDRYYHVIALFLFEMKIHHPIMK 120
Y R + + + PI K
Sbjct: 419 YARLVEAVYKVILARDFNQPIKK 487
Score = 47.8 bits (112), Expect = 3e-06
Identities = 21/41 (51%), Positives = 28/41 (68%)
Frame = +2
Query: 2 SGLPDELLCEILSFLPSEEFPSTSLLSKRWRRVWLGMPNAD 42
S LPD +LC ILS L ++E +TS+LSKRW +W +P D
Sbjct: 260 SSLPDPILCHILSLLTTKEAVTTSILSKRWIPLWRSVPTLD 382
>AV428270
Length = 428
Score = 51.6 bits (122), Expect = 2e-07
Identities = 38/131 (29%), Positives = 66/131 (50%), Gaps = 1/131 (0%)
Frame = +1
Query: 209 KILSMSPLLEDLLLKDLQVT-DNTLAHDDAAALKPFPKLLRADVSESSISAFLLPLKLFY 267
+ L+ P+LE+L + + +T +++ A PKL+RAD+ S + L+ FY
Sbjct: 1 EFLAGCPILENLKARRIWITGESSFYRGGELADLGLPKLIRADIVRSRLPM----LQPFY 168
Query: 268 NVQFLRSQVLLQTLEQDFSTTQFLNLTHMDLIFHDGYYWISLMKFICACPSLQTLTIHNI 327
NV FLR + E ++ F NLTH++L+ + Y + L++ + CP+LQ+L +
Sbjct: 169 NVAFLRVDIE----EVHYAFPYFHNLTHLELVI-ELYNSLVLVEMLKHCPNLQSLVFERL 333
Query: 328 GGDYDDHNNNW 338
NW
Sbjct: 334 DEAQRCDPENW 366
>TC11299
Length = 498
Score = 51.6 bits (122), Expect = 2e-07
Identities = 22/46 (47%), Positives = 32/46 (68%)
Frame = +1
Query: 1 MSGLPDELLCEILSFLPSEEFPSTSLLSKRWRRVWLGMPNADRISI 46
+S LPDE++C ILSFLP+E +T++LSKRW +W + D S+
Sbjct: 91 ISALPDEIICHILSFLPTENAVATAVLSKRWTHLWRSVSALDFSSV 228
Score = 49.7 bits (117), Expect = 7e-07
Identities = 21/39 (53%), Positives = 28/39 (70%)
Frame = +1
Query: 39 PNADRISILPDELLCRILSLLPAKQIMVTSLLSRRWRSL 77
P DRIS LPDE++C ILS LP + + T++LS+RW L
Sbjct: 76 PMVDRISALPDEIICHILSFLPTENAVATAVLSKRWTHL 192
>AU251749
Length = 362
Score = 32.7 bits (73), Expect(2) = 2e-05
Identities = 18/31 (58%), Positives = 21/31 (67%)
Frame = +2
Query: 42 DRISILPDELLCRILSLLPAKQIMVTSLLSR 72
D IS LPD +L ILS LP KQ++ TS SR
Sbjct: 137 DWISALPDTILNFILSFLPTKQVVATSGGSR 229
Score = 31.2 bits (69), Expect(2) = 2e-05
Identities = 19/50 (38%), Positives = 27/50 (54%), Gaps = 1/50 (2%)
Frame = +1
Query: 72 RRWRSLRPRMTEINIDDTSYIHDRDAYDRYYH-VIALFLFEMKIHHPIMK 120
+RW+ L + ++DD + DR+AY R V ALF +HHPI K
Sbjct: 214 KRWKPLWRSVPTRDLDDGRFWRDREAYARLVQGVNALF---RDLHHPIPK 354
Score = 28.1 bits (61), Expect = 2.2
Identities = 13/25 (52%), Positives = 19/25 (76%)
Frame = +2
Query: 1 MSGLPDELLCEILSFLPSEEFPSTS 25
+S LPD +L ILSFLP+++ +TS
Sbjct: 143 ISALPDTILNFILSFLPTKQVVATS 217
>BP063614
Length = 508
Score = 40.8 bits (94), Expect = 3e-04
Identities = 20/45 (44%), Positives = 28/45 (61%)
Frame = +2
Query: 40 NADRISILPDELLCRILSLLPAKQIMVTSLLSRRWRSLRPRMTEI 84
N DR+S LPD +L ILS + AK + T +LS RW+ L R+ +
Sbjct: 161 NKDRLSDLPDCILLHILSFVKAKAAVQTCILSTRWKDLWKRLPSL 295
Score = 37.4 bits (85), Expect = 0.004
Identities = 16/40 (40%), Positives = 26/40 (65%)
Frame = +2
Query: 1 MSGLPDELLCEILSFLPSEEFPSTSLLSKRWRRVWLGMPN 40
+S LPD +L ILSF+ ++ T +LS RW+ +W +P+
Sbjct: 173 LSDLPDCILLHILSFVKAKAAVQTCILSTRWKDLWKRLPS 292
>BI420296
Length = 592
Score = 39.7 bits (91), Expect = 7e-04
Identities = 18/55 (32%), Positives = 31/55 (55%)
Frame = +2
Query: 37 GMPNADRISILPDELLCRILSLLPAKQIMVTSLLSRRWRSLRPRMTEINIDDTSY 91
G DR+S LPD +L +L + KQ + T +LS RW+ L +T + ++ + +
Sbjct: 179 GKEERDRLSDLPDLVLLHVLKFMSTKQAVQTCVLSTRWKDLWKGVTTLALNSSDF 343
Score = 36.2 bits (82), Expect = 0.008
Identities = 14/38 (36%), Positives = 25/38 (64%)
Frame = +2
Query: 1 MSGLPDELLCEILSFLPSEEFPSTSLLSKRWRRVWLGM 38
+S LPD +L +L F+ +++ T +LS RW+ +W G+
Sbjct: 200 LSDLPDLVLLHVLKFMSTKQAVQTCVLSTRWKDLWKGV 313
>BP071245
Length = 552
Score = 39.7 bits (91), Expect = 7e-04
Identities = 20/52 (38%), Positives = 30/52 (57%)
Frame = +1
Query: 40 NADRISILPDELLCRILSLLPAKQIMVTSLLSRRWRSLRPRMTEINIDDTSY 91
N DR+S LPD +L ILS + AK + T LS RW+ L R+ + + + +
Sbjct: 376 NKDRLSDLPDCVLLHILSFVNAKYAVQTCTLSTRWKDLWKRLPSLILHSSDF 531
Score = 35.0 bits (79), Expect = 0.018
Identities = 16/40 (40%), Positives = 25/40 (62%)
Frame = +1
Query: 1 MSGLPDELLCEILSFLPSEEFPSTSLLSKRWRRVWLGMPN 40
+S LPD +L ILSF+ ++ T LS RW+ +W +P+
Sbjct: 388 LSDLPDCVLLHILSFVNAKYAVQTCTLSTRWKDLWKRLPS 507
>AV419392
Length = 318
Score = 37.0 bits (84), Expect = 0.005
Identities = 19/51 (37%), Positives = 27/51 (52%)
Frame = +3
Query: 55 ILSLLPAKQIMVTSLLSRRWRSLRPRMTEINIDDTSYIHDRDAYDRYYHVI 105
ILS + K M S+LS+RWRSL + +N D S+ H + HV+
Sbjct: 6 ILSYVETKDAMQCSVLSKRWRSLWRALPVLNFDSASFTHYSSFANFVLHVL 158
Score = 32.0 bits (71), Expect = 0.15
Identities = 12/28 (42%), Positives = 20/28 (70%)
Frame = +3
Query: 12 ILSFLPSEEFPSTSLLSKRWRRVWLGMP 39
ILS++ +++ S+LSKRWR +W +P
Sbjct: 6 ILSYVETKDAMQCSVLSKRWRSLWRALP 89
>AV423751
Length = 254
Score = 35.8 bits (81), Expect = 0.011
Identities = 25/59 (42%), Positives = 33/59 (55%)
Frame = +2
Query: 162 VLNSTALVVLKLNALTIDYVHRSSTNLPSLKILHLTQVHFLKLKFLIKILSMSPLLEDL 220
+ + T LVVLKL L + + SS +LP LK+LHL + L +ILS LEDL
Sbjct: 77 MFSCTTLVVLKLEGLELK-ANFSSVDLPFLKVLHLQDLLLQDEGCLAEILSGCLALEDL 250
>BP050969
Length = 557
Score = 35.0 bits (79), Expect = 0.018
Identities = 19/57 (33%), Positives = 32/57 (55%), Gaps = 7/57 (12%)
Frame = -3
Query: 290 FLNLTHMDLIFHD-GYYWISLMKFICACPSLQTLTI-----HNIG-GDYDDHNNNWP 339
F NLTH+++++ + W +++F+ CP LQTL I H G G+Y+ +P
Sbjct: 300 FHNLTHVEIVYANFNEDWFEVVEFLTHCPKLQTLVISQPKFHEYGLGEYEVGFGQYP 130
>TC17934 similar to PIR|T06667|T06667 argininosuccinate synthase -
Arabidopsis thaliana {Arabidopsis
thaliana;} , partial (11%)
Length = 564
Score = 35.0 bits (79), Expect = 0.018
Identities = 16/32 (50%), Positives = 21/32 (65%)
Frame = -1
Query: 4 LPDELLCEILSFLPSEEFPSTSLLSKRWRRVW 35
LPD + ILS LP E TS+LS++WR +W
Sbjct: 555 LPDGIPGAILSKLPINEAAKTSILSRKWRYMW 460
Score = 32.3 bits (72), Expect = 0.12
Identities = 16/32 (50%), Positives = 21/32 (65%)
Frame = -1
Query: 44 ISILPDELLCRILSLLPAKQIMVTSLLSRRWR 75
I+ LPD + ILS LP + TS+LSR+WR
Sbjct: 564 INQLPDGIPGAILSKLPINEAAKTSILSRKWR 469
>TC14800 weakly similar to UP|Q9FHP3 (Q9FHP3) Genomic DNA, chromosome 5,
TAC clone:K14B20, partial (7%)
Length = 782
Score = 33.9 bits (76), Expect = 0.040
Identities = 14/30 (46%), Positives = 21/30 (69%)
Frame = +3
Query: 47 LPDELLCRILSLLPAKQIMVTSLLSRRWRS 76
LPDEL+ ILS LP K ++ ++S+ W+S
Sbjct: 48 LPDELIIEILSWLPVKSLLQFRVVSKTWKS 137
Score = 32.3 bits (72), Expect = 0.12
Identities = 14/29 (48%), Positives = 20/29 (68%)
Frame = +3
Query: 4 LPDELLCEILSFLPSEEFPSTSLLSKRWR 32
LPDEL+ EILS+LP + ++SK W+
Sbjct: 48 LPDELIIEILSWLPVKSLLQFRVVSKTWK 134
>TC14802 weakly similar to UP|CAD56661 (CAD56661) S locus F-box (SLF)-S4
protein, partial (10%)
Length = 551
Score = 33.9 bits (76), Expect = 0.040
Identities = 14/30 (46%), Positives = 21/30 (69%)
Frame = +3
Query: 47 LPDELLCRILSLLPAKQIMVTSLLSRRWRS 76
LPDEL+ ILS LP K ++ ++S+ W+S
Sbjct: 21 LPDELIIEILSWLPVKSLLQFRVVSKTWKS 110
Score = 32.3 bits (72), Expect = 0.12
Identities = 14/29 (48%), Positives = 20/29 (68%)
Frame = +3
Query: 4 LPDELLCEILSFLPSEEFPSTSLLSKRWR 32
LPDEL+ EILS+LP + ++SK W+
Sbjct: 21 LPDELIIEILSWLPVKSLLQFRVVSKTWK 107
>BG662160
Length = 445
Score = 33.5 bits (75), Expect = 0.052
Identities = 15/38 (39%), Positives = 23/38 (60%)
Frame = +2
Query: 40 NADRISILPDELLCRILSLLPAKQIMVTSLLSRRWRSL 77
N+ R LP+EL ILS LP K ++ +S+ W+S+
Sbjct: 2 NSARAQFLPEELRLEILSWLPVKSLVRFRCVSKSWKSI 115
Score = 29.3 bits (64), Expect = 0.99
Identities = 13/31 (41%), Positives = 19/31 (60%)
Frame = +2
Query: 4 LPDELLCEILSFLPSEEFPSTSLLSKRWRRV 34
LP+EL EILS+LP + +SK W+ +
Sbjct: 23 LPEELRLEILSWLPVKSLVRFRCVSKSWKSI 115
>AW720409
Length = 575
Score = 33.1 bits (74), Expect = 0.068
Identities = 16/32 (50%), Positives = 21/32 (65%)
Frame = +3
Query: 46 ILPDELLCRILSLLPAKQIMVTSLLSRRWRSL 77
+LP EL ILS LP K +M S +S+ W+SL
Sbjct: 90 VLPFELWIEILSWLPVKTLMRFSCVSKSWKSL 185
Score = 28.1 bits (61), Expect = 2.2
Identities = 14/29 (48%), Positives = 18/29 (61%)
Frame = +3
Query: 4 LPDELLCEILSFLPSEEFPSTSLLSKRWR 32
LP EL EILS+LP + S +SK W+
Sbjct: 93 LPFELWIEILSWLPVKTLMRFSCVSKSWK 179
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.326 0.140 0.428
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,039,842
Number of Sequences: 28460
Number of extensions: 111016
Number of successful extensions: 990
Number of sequences better than 10.0: 75
Number of HSP's better than 10.0 without gapping: 940
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 984
length of query: 339
length of database: 4,897,600
effective HSP length: 91
effective length of query: 248
effective length of database: 2,307,740
effective search space: 572319520
effective search space used: 572319520
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 55 (25.8 bits)
Medicago: description of AC146632.7