BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146632.1 + phase: 0
(432 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC11835 similar to UP|Q9LG00 (Q9LG00) F20N2.10, partial (35%) 236 5e-63
AW720424 160 4e-40
AV771243 155 1e-38
AV419310 129 1e-30
TC19522 similar to UP|Q94AB4 (Q94AB4) AT3g13340/MDC11_13, partia... 126 8e-30
AV424516 116 6e-27
BP043927 112 9e-26
BP077941 98 2e-21
TC9102 similar to UP|Q94JT6 (Q94JT6) At1g78070/F28K19_28, partia... 94 6e-20
TC11756 weakly similar to GB|AAH01494.1|16306637|BC001494 U5 snR... 44 4e-05
TC19491 similar to GB|AAP37692.1|30725340|BT008333 At4g29830 {Ar... 44 5e-05
TC14937 similar to UP|Q9FN19 (Q9FN19) Genomic DNA, chromosome 5,... 41 3e-04
TC16076 weakly similar to GB|AAG32022.1|11141605|AF277458 LEUNIG... 38 0.003
TC13023 similar to UP|Q8L830 (Q8L830) WD40-repeat protein (Fragm... 38 0.004
BP062627 37 0.008
BP038344 37 0.008
TC12572 similar to PIR|T46032|T46032 WD-40 repeat regulatory pro... 35 0.031
TC10816 homologue to UP|CAE45585 (CAE45585) Coatomer alpha subun... 33 0.091
TC8529 homologue to UP|Q94KS2 (Q94KS2) TGF-beta receptor-interac... 33 0.12
AU089179 32 0.16
>TC11835 similar to UP|Q9LG00 (Q9LG00) F20N2.10, partial (35%)
Length = 769
Score = 236 bits (602), Expect = 5e-63
Identities = 104/156 (66%), Positives = 129/156 (82%)
Frame = +3
Query: 275 KLMTVVGDNLEGLLVDPQNGKTVATLSGHQDYSFASAWHPNGLAFATGNQDKTCRVWDAR 334
KL+ +VGDN E +LVD Q GKT+ +L GH D+SFASAWHP+G+ FATGNQDKTCR+WD R
Sbjct: 12 KLLLIVGDNPESMLVDSQTGKTIISLCGHLDFSFASAWHPDGMTFATGNQDKTCRIWDMR 191
Query: 335 NVSSPIAILKNNLGASRSIRFSSDGQFMVVAEPADFVHVYSTTANYEKSQEIDFFGEISG 394
N+S +A+LK NLGA RSIR++SDGQ++ +AEPADFVHVY + YE+ QEIDFFGEISG
Sbjct: 192 NLSKSVAVLKGNLGAIRSIRYTSDGQYLAMAEPADFVHVYDVKSGYEREQEIDFFGEISG 371
Query: 395 VSVSPDDECMYIGIWDRTYASLLQYNRRHSYHYLDS 430
+S SPD E ++IG+WDRTY SLL+Y RR +Y YLDS
Sbjct: 372 ISFSPDTESLFIGVWDRTYGSLLEYGRRQNYSYLDS 479
>AW720424
Length = 532
Score = 160 bits (405), Expect = 4e-40
Identities = 80/129 (62%), Positives = 96/129 (74%)
Frame = +2
Query: 4 DDFFDDVDEQEEFDGAAEDVPLDEYEMQLTKVTDTSAAHARKGKDIQGIPWEMLNITRES 63
+D DDV E D D DEY+ +KV DT+AAHAR+G+DIQGIPW+ L+ITRE
Sbjct: 152 EDDMDDVIVNRETDDLESDG--DEYDYSSSKVVDTTAAHARRGQDIQGIPWDSLSITRER 325
Query: 64 YRLTRLEQYRNFENILTSGETADKDCKQVQKGDKYYEFFYNTRTVKPTILHFQLRNLVWA 123
YR TRLEQY+N+ENI SGE + KDC +KG +YEF N+R+VK TILHFQLRNLVWA
Sbjct: 326 YRQTRLEQYKNYENIPHSGEKSGKDCINTKKGFSFYEFQKNSRSVKSTILHFQLRNLVWA 505
Query: 124 TSKHDVYLV 132
TSKHDVYL+
Sbjct: 506 TSKHDVYLM 532
>AV771243
Length = 520
Score = 155 bits (392), Expect = 1e-38
Identities = 79/132 (59%), Positives = 96/132 (71%)
Frame = +1
Query: 4 DDFFDDVDEQEEFDGAAEDVPLDEYEMQLTKVTDTSAAHARKGKDIQGIPWEMLNITRES 63
+D DDV E D D DEY+ +KV DT+AAHAR+G+DIQGIPW+ L+ITRE
Sbjct: 130 EDDMDDVIVNRETDDLESDG--DEYDYSSSKVVDTTAAHARRGQDIQGIPWDSLSITRER 303
Query: 64 YRLTRLEQYRNFENILTSGETADKDCKQVQKGDKYYEFFYNTRTVKPTILHFQLRNLVWA 123
YR TRLEQY+N+ENI SGE + KDC +KG +YEF N+R+VK TILHFQLRN V A
Sbjct: 304 YRQTRLEQYKNYENIPHSGEKSGKDCINTKKGFSFYEFQKNSRSVKSTILHFQLRNSVRA 483
Query: 124 TSKHDVYLVSNY 135
TSKHDVYL+S +
Sbjct: 484 TSKHDVYLMSQF 519
>AV419310
Length = 412
Score = 129 bits (323), Expect = 1e-30
Identities = 60/120 (50%), Positives = 85/120 (70%)
Frame = +3
Query: 114 HFQLRNLVWATSKHDVYLVSNYSIKHWSSLSGNLSEIINFLGHVAPTEVLQCAGNQMEGF 173
+ LRNL+WATSKHDVYL+ NYS+ HWSSL E++N + P L+ G +
Sbjct: 48 YLMLRNLLWATSKHDVYLMQNYSVMHWSSLLRKGKEVLNVAKPIIPN--LKRTGFLAQPV 221
Query: 174 SQTQISTLAVKDNFLVAGGFQGELTCKRLDKKGVSFCTRTTHDDNAITNAVEIYESLSGA 233
S+ Q+ST+AVK+N +VAGGF GE+ CK L++ GV+FC++ T DDNAITNAV+++ + G+
Sbjct: 222 SRVQVSTMAVKENLMVAGGFHGEVICKNLNQSGVAFCSKITTDDNAITNAVDVFRNPGGS 401
>TC19522 similar to UP|Q94AB4 (Q94AB4) AT3g13340/MDC11_13, partial (18%)
Length = 527
Score = 126 bits (316), Expect = 8e-30
Identities = 62/120 (51%), Positives = 85/120 (70%), Gaps = 5/120 (4%)
Frame = +3
Query: 2 EEDDFFDDVDEQEEFDG-----AAEDVPLDEYEMQLTKVTDTSAAHARKGKDIQGIPWEM 56
++++ ++V++ F G + D DEYE ++TDT+AA ARKGKDIQGIP +
Sbjct: 168 DDNEMAEEVEDDAYFRGQVFVDSESDDDDDEYESLENRITDTTAAEARKGKDIQGIPRDR 347
Query: 57 LNITRESYRLTRLEQYRNFENILTSGETADKDCKQVQKGDKYYEFFYNTRTVKPTILHFQ 116
L+I+RE YR TRLEQY+N+ENI SGE ++K+CK KG YY+F+ NTR+VK TILHFQ
Sbjct: 348 LSISREKYRQTRLEQYKNYENIPQSGEQSEKECKPTDKGGAYYDFWQNTRSVKSTILHFQ 527
>AV424516
Length = 256
Score = 116 bits (291), Expect = 6e-27
Identities = 53/85 (62%), Positives = 65/85 (76%)
Frame = +1
Query: 225 EIYESLSGATHFIAANNDSGVREYDIEKFQLLNQLHFPWPVNHTSISPDRKLMTVVGDNL 284
+IY+ SGA HF+A+NND VR++D+E+FQL F WPVNHTS+SPD KL+ +VGDN
Sbjct: 1 QIYDHPSGAVHFMASNNDCVVRDFDLERFQLSKHFFFSWPVNHTSLSPDGKLLVIVGDNP 180
Query: 285 EGLLVDPQNGKTVATLSGHQDYSFA 309
EG+LVD Q GKTV L GH DYSFA
Sbjct: 181 EGMLVDSQTGKTVMPLCGHLDYSFA 255
>BP043927
Length = 513
Score = 112 bits (281), Expect = 9e-26
Identities = 51/83 (61%), Positives = 65/83 (77%)
Frame = +1
Query: 36 TDTSAAHARKGKDIQGIPWEMLNITRESYRLTRLEQYRNFENILTSGETADKDCKQVQKG 95
TDTSA+ AR GKDIQGIPWE LN +R+ YR+TRL+QY N+EN+ S E K+C +VQKG
Sbjct: 244 TDTSASEARNGKDIQGIPWEKLNYSRDEYRVTRLKQYMNYENLSRSREDLHKECLEVQKG 423
Query: 96 DKYYEFFYNTRTVKPTILHFQLR 118
+Y+F +NTR VK TILHFQ++
Sbjct: 424 KSFYDFQFNTRLVKSTILHFQVQ 492
>BP077941
Length = 368
Score = 98.2 bits (243), Expect = 2e-21
Identities = 45/63 (71%), Positives = 50/63 (78%)
Frame = -2
Query: 368 ADFVHVYSTTANYEKSQEIDFFGEISGVSVSPDDECMYIGIWDRTYASLLQYNRRHSYHY 427
ADFVHVY ++K QEIDFFGEISGVS SPD E ++IG+WDRTY SLLQYNRR S Y
Sbjct: 367 ADFVHVYDAKNGFQKEQEIDFFGEISGVSFSPDTESLFIGVWDRTYGSLLQYNRRRSCMY 188
Query: 428 LDS 430
LDS
Sbjct: 187 LDS 179
>TC9102 similar to UP|Q94JT6 (Q94JT6) At1g78070/F28K19_28, partial (15%)
Length = 630
Score = 93.6 bits (231), Expect = 6e-20
Identities = 38/71 (53%), Positives = 56/71 (78%)
Frame = +1
Query: 362 MVVAEPADFVHVYSTTANYEKSQEIDFFGEISGVSVSPDDECMYIGIWDRTYASLLQYNR 421
+ +AEPADF+HV+ + + YE+ QEID FGEI+GVS SPD E +++G+ DRTY SLL++ R
Sbjct: 1 LAMAEPADFIHVFDSHSGYEQCQEIDIFGEIAGVSFSPDPEALFVGVADRTYGSLLEFTR 180
Query: 422 RHSYHYLDSYF 432
++HY+D+ F
Sbjct: 181 NRNHHYVDTMF 213
>TC11756 weakly similar to GB|AAH01494.1|16306637|BC001494 U5 snRNP-specific
40 kDa protein (hPrp8-binding) {Homo sapiens;} , partial
(43%)
Length = 719
Score = 44.3 bits (103), Expect = 4e-05
Identities = 26/128 (20%), Positives = 53/128 (41%)
Frame = +1
Query: 297 VATLSGHQDYSFASAWHPNGLAFATGNQDKTCRVWDARNVSSPIAILKNNLGASRSIRFS 356
+ L+GHQ + ++P G A+G+ D+ +W+ +LK + A + ++
Sbjct: 283 IMLLTGHQSVIYTMKFNPAGTVIASGSHDREIFLWNVHGECKNFMVLKGHKNAVLDLHWT 462
Query: 357 SDGQFMVVAEPADFVHVYSTTANYEKSQEIDFFGEISGVSVSPDDECMYIGIWDRTYASL 416
+DG +V A P V V+ + + ++ ++ S + + D A L
Sbjct: 463 TDGTQIVSASPDKTVRVWDVETGKQVKKMVEHLSYVNSCCPSRRGPPLVVSGSDDGTAKL 642
Query: 417 LQYNRRHS 424
+R S
Sbjct: 643 WDMRQRGS 666
Score = 30.8 bits (68), Expect = 0.45
Identities = 13/38 (34%), Positives = 18/38 (47%)
Frame = +1
Query: 295 KTVATLSGHQDYSFASAWHPNGLAFATGNQDKTCRVWD 332
K L GH++ W +G + + DKT RVWD
Sbjct: 406 KNFMVLKGHKNAVLDLHWTTDGTQIVSASPDKTVRVWD 519
>TC19491 similar to GB|AAP37692.1|30725340|BT008333 At4g29830 {Arabidopsis
thaliana;}, partial (39%)
Length = 527
Score = 43.9 bits (102), Expect = 5e-05
Identities = 26/74 (35%), Positives = 37/74 (49%), Gaps = 1/74 (1%)
Frame = +1
Query: 260 HFPWPVNHTSISP-DRKLMTVVGDNLEGLLVDPQNGKTVATLSGHQDYSFASAWHPNGLA 318
HF PV SP D +L+ D+ + D + V T+SGH + P+G A
Sbjct: 13 HF-MPVRSLVYSPYDPRLLFTASDDGNVHMYDAEGKALVGTMSGHASWVLCVDVSPDGGA 189
Query: 319 FATGNQDKTCRVWD 332
ATG+ D+T R+WD
Sbjct: 190 IATGSSDRTVRLWD 231
>TC14937 similar to UP|Q9FN19 (Q9FN19) Genomic DNA, chromosome 5, TAC
clone:K8K14 (AT5g67320/K8K14_4), partial (19%)
Length = 1089
Score = 41.2 bits (95), Expect = 3e-04
Identities = 21/72 (29%), Positives = 40/72 (55%), Gaps = 1/72 (1%)
Frame = +1
Query: 264 PVNHTSISPDRKLMTVVGD-NLEGLLVDPQNGKTVATLSGHQDYSFASAWHPNGLAFATG 322
P + +P++KL+ + L D + GK + +L+GH+D ++ A+ PNG A+G
Sbjct: 13 PTGPGTSNPNKKLVLASASFDSTVKLWDVEVGKLIYSLNGHRDGVYSVAFSPNGEYLASG 192
Query: 323 NQDKTCRVWDAR 334
+ DK+ +W +
Sbjct: 193 SPDKSIHIWSLK 228
>TC16076 weakly similar to GB|AAG32022.1|11141605|AF277458 LEUNIG
{Arabidopsis thaliana;} , partial (24%)
Length = 1048
Score = 38.1 bits (87), Expect = 0.003
Identities = 23/85 (27%), Positives = 40/85 (47%)
Frame = +3
Query: 271 SPDRKLMTVVGDNLEGLLVDPQNGKTVATLSGHQDYSFASAWHPNGLAFATGNQDKTCRV 330
S D K++ GD+ + +L + +T +T H+ + PN AT + DK+ R+
Sbjct: 21 SSDGKMLASAGDDKKVVLWNMDTLQTESTPEQHKSVISDVRFRPNSSQLATASIDKSVRL 200
Query: 331 WDARNVSSPIAILKNNLGASRSIRF 355
WDA N + + + A S+ F
Sbjct: 201 WDAANPTYCVQEYNGHSSAIMSLDF 275
>TC13023 similar to UP|Q8L830 (Q8L830) WD40-repeat protein (Fragment),
partial (18%)
Length = 591
Score = 37.7 bits (86), Expect = 0.004
Identities = 21/68 (30%), Positives = 32/68 (46%)
Frame = +1
Query: 265 VNHTSISPDRKLMTVVGDNLEGLLVDPQNGKTVATLSGHQDYSFASAWHPNGLAFATGNQ 324
V ++SPD L+ G + + + D K V + GH+ + HP+G ATG
Sbjct: 292 VTALALSPDDNLLFSSGHSRQIRVWDLSTLKCVRSWKGHEGPVMCMSCHPSGGLLATGGA 471
Query: 325 DKTCRVWD 332
D+ VWD
Sbjct: 472 DRKVLVWD 495
>BP062627
Length = 564
Score = 36.6 bits (83), Expect = 0.008
Identities = 17/59 (28%), Positives = 33/59 (55%), Gaps = 10/59 (16%)
Frame = +1
Query: 314 PNGLAFATGNQDKTCRVWDARNVSSP----------IAILKNNLGASRSIRFSSDGQFM 362
P GL FATG D R+W+ ++VS+ +A L+++ G+ +R++ G+++
Sbjct: 349 PGGLRFATGGGDHKVRIWNMKSVSTNMENDDASQRLLATLRDHFGSVNCVRWAKHGRYV 525
>BP038344
Length = 525
Score = 36.6 bits (83), Expect = 0.008
Identities = 23/89 (25%), Positives = 39/89 (42%)
Frame = +2
Query: 271 SPDRKLMTVVGDNLEGLLVDPQNGKTVATLSGHQDYSFASAWHPNGLAFATGNQDKTCRV 330
S D + D+ + D G V TL GH F ++P +G+ D+T RV
Sbjct: 251 SSDSHYICSASDDRTLRIWDATGGDCVKTLRGHTHAVFCVNFNPQSNYIVSGSFDETVRV 430
Query: 331 WDARNVSSPIAILKNNLGASRSIRFSSDG 359
W+ + I+ + + + S+ F+ DG
Sbjct: 431 WEVKTGRCIHTIIAHTMPVT-SVHFNRDG 514
Score = 30.8 bits (68), Expect = 0.45
Identities = 17/75 (22%), Positives = 33/75 (43%)
Frame = +2
Query: 300 LSGHQDYSFASAWHPNGLAFATGNQDKTCRVWDARNVSSPIAILKNNLGASRSIRFSSDG 359
L+GH + AW + + + D+T R+WDA + L+ + A + F+
Sbjct: 212 LTGHSEGISDLAWSSDSHYICSASDDRTLRIWDATG-GDCVKTLRGHTHAVFCVNFNPQS 388
Query: 360 QFMVVAEPADFVHVY 374
++V + V V+
Sbjct: 389 NYIVSGSFDETVRVW 433
>TC12572 similar to PIR|T46032|T46032 WD-40 repeat regulatory protein tup1
homolog - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (51%)
Length = 770
Score = 34.7 bits (78), Expect = 0.031
Identities = 17/73 (23%), Positives = 35/73 (47%)
Frame = +3
Query: 292 QNGKTVATLSGHQDYSFASAWHPNGLAFATGNQDKTCRVWDARNVSSPIAILKNNLGASR 351
++GK + L H D A ++ +G + + D CR+WDA ++ +
Sbjct: 3 KSGKCLKVLPAHSDPVTAVDFNRDGSLIVSSSYDGLCRIWDASTGHCMKTLIDDENPPVS 182
Query: 352 SIRFSSDGQFMVV 364
++FS + +F++V
Sbjct: 183 FVKFSPNAKFILV 221
Score = 29.3 bits (64), Expect = 1.3
Identities = 15/42 (35%), Positives = 20/42 (46%), Gaps = 2/42 (4%)
Frame = +3
Query: 292 QNGKTVATLSGHQDYSFASAWHPNGLAFATG--NQDKTCRVW 331
Q K V L GH D + + HP A+G DKT ++W
Sbjct: 393 QTRKIVQKLEGHSDTVVSVSCHPTENMIASGALGNDKTVKIW 518
>TC10816 homologue to UP|CAE45585 (CAE45585) Coatomer alpha subunit-like
protein, partial (20%)
Length = 979
Score = 33.1 bits (74), Expect = 0.091
Identities = 13/41 (31%), Positives = 22/41 (52%)
Frame = +1
Query: 292 QNGKTVATLSGHQDYSFASAWHPNGLAFATGNQDKTCRVWD 332
Q+ ++ L+GH Y + +HP + + D+T RVWD
Sbjct: 607 QSRTCISVLTGHNHYVMCALFHPREDLVVSASLDQTVRVWD 729
>TC8529 homologue to UP|Q94KS2 (Q94KS2) TGF-beta receptor-interacting
protein 1, partial (44%)
Length = 650
Score = 32.7 bits (73), Expect = 0.12
Identities = 26/122 (21%), Positives = 47/122 (38%), Gaps = 14/122 (11%)
Frame = +1
Query: 223 AVEIYESLSGATHFIAANNDSGVREYDIEKFQLLNQLHFPWPVNHTSISP---------- 272
A+ + +HF+ + D R +D L+ PVN ++SP
Sbjct: 40 AITSLSKSADGSHFVTGSLDKSARLWDTRTLTLIKTYVTERPVNAVAMSPLLDHVVLGGG 219
Query: 273 -DRKLMTVV---GDNLEGLLVDPQNGKTVATLSGHQDYSFASAWHPNGLAFATGNQDKTC 328
D +T E D + + + GH A A++P+G +F++G +D
Sbjct: 220 QDASAVTTTDHRAGKFEAKFYDKILQEEIGGVKGHFGPINALAFNPDGKSFSSGGEDGYV 399
Query: 329 RV 330
R+
Sbjct: 400 RL 405
Score = 30.8 bits (68), Expect = 0.45
Identities = 13/37 (35%), Positives = 22/37 (59%)
Frame = +1
Query: 301 SGHQDYSFASAWHPNGLAFATGNQDKTCRVWDARNVS 337
SGH+ + + +G F TG+ DK+ R+WD R ++
Sbjct: 25 SGHKKAITSLSKSADGSHFVTGSLDKSARLWDTRTLT 135
>AU089179
Length = 340
Score = 32.3 bits (72), Expect = 0.16
Identities = 22/66 (33%), Positives = 37/66 (55%), Gaps = 5/66 (7%)
Frame = +3
Query: 303 HQDYSFASAWHPNGLAFATGNQDKTCRV--WDARNVSSPIAILKNNLGASRSIR---FSS 357
H+ A A++ +G A ATG +D + RV W V IL+++ S S++ FSS
Sbjct: 3 HEGQQLALAFNNDGTALATGGEDGSLRVFKWPXMKV-----ILEHSTAHSSSVKDLHFSS 167
Query: 358 DGQFMV 363
+G+++V
Sbjct: 168 NGKWIV 185
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.317 0.133 0.399
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,127,931
Number of Sequences: 28460
Number of extensions: 92837
Number of successful extensions: 530
Number of sequences better than 10.0: 66
Number of HSP's better than 10.0 without gapping: 514
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 528
length of query: 432
length of database: 4,897,600
effective HSP length: 93
effective length of query: 339
effective length of database: 2,250,820
effective search space: 763027980
effective search space used: 763027980
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 57 (26.6 bits)
Medicago: description of AC146632.1