BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146631.6 - phase: 0 /pseudo
(586 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC15936 similar to UP|BAD05166 (BAD05166) Acid phosphatase, part... 128 2e-30
AV422369 113 8e-26
TC17734 similar to UP|Q84KZ2 (Q84KZ2) Purple acid phosphatase, p... 108 2e-24
AV427288 73 1e-13
TC12436 similar to UP|Q93XG4 (Q93XG4) Phytase, partial (34%) 59 3e-09
AW719640 55 4e-08
TC18698 45 3e-05
TC16271 similar to GB|AAM15909.1|20257479|AF492660 purple acid p... 33 0.13
AV416789 33 0.13
BP038458 33 0.17
TC9054 31 0.64
TC10120 similar to UP|Q9LTA2 (Q9LTA2) Similarity to AT-hook DNA-... 30 1.1
TC20218 29 1.9
TC14503 28 3.2
CN825496 28 3.2
CB827350 28 4.1
TC12473 similar to UP|Q9M068 (Q9M068) Protein kinase-like protei... 28 5.4
TC17166 homologue to UP|Q84L15 (Q84L15) Cytosolic glutamine synt... 28 5.4
TC12483 weakly similar to UP|Q40552 (Q40552) Pistil extensin lik... 28 5.4
AV417488 27 7.1
>TC15936 similar to UP|BAD05166 (BAD05166) Acid phosphatase, partial (88%)
Length = 1324
Score = 128 bits (322), Expect = 2e-30
Identities = 97/312 (31%), Positives = 147/312 (47%), Gaps = 13/312 (4%)
Frame = +2
Query: 228 GYIHSALMTGLKPSSAYSYRYGSNSADWSEQTKFSTPPAGGSDE-LKFISFGDMGKTPLD 286
GYIH L+ GL+ + Y YR GS D S + F TPP D KF GD+G+T
Sbjct: 455 GYIHHCLIEGLQYKTKYYYRIGSG--DSSREFWFETPPKVEPDAPYKFGIIGDLGQTFNS 628
Query: 287 ASE-EHYIQPGALSVIKAIANEVNSNNVNSVFHIGDISYA-----TGFLAEWDFFLNLIS 340
S +HYIQ GA +V+ +GD+SYA T WD + +
Sbjct: 629 LSTLDHYIQSGAQAVL----------------FLGDLSYADRYQYTDVGLRWDTWGRFVE 760
Query: 341 PVASRVSYMTAIGNHERDYIDSGSVYVTPDSGGECGVPYETYF-----PMPTAAKDKP-W 394
+ ++ + GNHE DY+ Y+ GE VP++ Y P + P W
Sbjct: 761 KSTAYQPWIWSAGNHEIDYMP----YM-----GEV-VPFKNYLHRYTTPYLASKSSSPLW 910
Query: 395 YSIEQGSVHFTVISTEHDWSENSEQYNWIKKDLASVNRQHTPWLIFMGHRPMYTSNNGFS 454
Y+I + S H V+S+ + + + Q+ W++++L V+R+ TPWLI + H P+Y SN
Sbjct: 911 YAIRRASAHVIVVSSYSPFVKYTPQWKWLEEELKRVDREKTPWLIVLMHVPLYNSNEAHY 1090
Query: 455 SKDQKFINAVEPLLLQNKVDLVLFGHVHNYERTCSVYQNKCKAIPIKDQKGVDTYDNRNY 514
+ + E ++ KVD++ GHVH YER+ + I G + Y +
Sbjct: 1091MEGESMRAVFESWFIKYKVDVIFAGHVHAYERSYRI-----SNIEYNITSG-NRYPVPDK 1252
Query: 515 SAPVHAVIGMAG 526
SAPV+ +G G
Sbjct: 1253SAPVYITVGDGG 1288
>AV422369
Length = 483
Score = 113 bits (283), Expect = 8e-26
Identities = 58/145 (40%), Positives = 86/145 (59%), Gaps = 6/145 (4%)
Frame = +1
Query: 208 TFSQEDMCSDPAKDFGWHDPGYIHSALMTGLKPSSAYSYRYG----SNSADWSEQTKFST 263
TF + MC PA+ GW DPGYIH++ + L P+ Y Y+ G + + WS++ +F
Sbjct: 52 TFDRNTMCGAPARTVGWRDPGYIHTSFLKDLWPNREYLYKLGHRLFNGTIIWSKEYQFKA 231
Query: 264 PPAGGSDEL-KFISFGDMGKTPLDASEEH-YIQPGALSVIKAIANEVNSNNVNSVFHIGD 321
P G + + + + FGDMGK D S E+ QPG+L K I ++ ++++ VFHIGD
Sbjct: 232 SPYPGQNSVQRVVIFGDMGKAEADGSNEYNNFQPGSLITTKQIIKDL--DDIDIVFHIGD 405
Query: 322 ISYATGFLAEWDFFLNLISPVASRV 346
I YA G+L++WD F I P+AS V
Sbjct: 406 ICYANGYLSQWDQFTAQIEPIASTV 480
>TC17734 similar to UP|Q84KZ2 (Q84KZ2) Purple acid phosphatase, partial
(49%)
Length = 694
Score = 108 bits (271), Expect = 2e-24
Identities = 73/233 (31%), Positives = 109/233 (46%), Gaps = 13/233 (5%)
Frame = +2
Query: 307 EVNSNNVNSVFHIGDISYATGFLAE----WDFFLNLISPVASRVSYMTAIGNHERDYIDS 362
E N N +V +GD+SYA + WD + + + ++ GNHE D+
Sbjct: 26 ETNPKNGQTVLFVGDLSYADNYPNHDNNRWDSWGRFVERSVAYQPWIWTAGNHELDF--- 196
Query: 363 GSVYVTPDSGGECGV-PYETYFPMPTAAKDKP---WYSIEQGSVHFTVISTEHDWSENSE 418
P+ G PY +P+P A WYSI++ S + V+S+ + + +
Sbjct: 197 -----APEIGETKPFKPYTRRYPVPYKASGSTQPFWYSIKRASAYIIVLSSYSAYGKYTP 361
Query: 419 QYNWIKKDLASVNRQHTPWLIFMGHRPMYTSNNGFSSKDQKFINAVEPLLLQNKVDLVLF 478
QY W++++L VNR TPWLI + H P Y S N + + EP ++ KVD+V
Sbjct: 362 QYKWLEQELPKVNRTETPWLIVLVHSPWYNSYNYHFLEGETMRVMFEPWFVKYKVDVVFA 541
Query: 479 GHVHNYERT---CSVYQNKCKAI--PIKDQKGVDTYDNRNYSAPVHAVIGMAG 526
GHVH YER+ ++ N I P+KDQ SAPV+ IG G
Sbjct: 542 GHVHAYERSERVSNIAYNVVNGICTPVKDQ-----------SAPVYITIGDGG 667
>AV427288
Length = 223
Score = 72.8 bits (177), Expect = 1e-13
Identities = 37/73 (50%), Positives = 51/73 (69%), Gaps = 4/73 (5%)
Frame = +2
Query: 9 KHV----FILLFFFLSNPSMSSTFPSSTLDLTVTHTKALHQNFTALSDFRLINRRILNDC 64
KHV +L FF S+ + + SS LDL V +T +H+NFTA+S+FRLINRRIL DC
Sbjct: 2 KHVPIVLLVLTFFLNSSTYATPSSSSSLLDLVVANTTTMHRNFTAVSNFRLINRRILKDC 181
Query: 65 SHLSPYLKLNITS 77
S SP++++N+TS
Sbjct: 182SASSPFVQVNVTS 220
>TC12436 similar to UP|Q93XG4 (Q93XG4) Phytase, partial (34%)
Length = 817
Score = 58.5 bits (140), Expect = 3e-09
Identities = 29/68 (42%), Positives = 42/68 (61%)
Frame = +3
Query: 424 KKDLASVNRQHTPWLIFMGHRPMYTSNNGFSSKDQKFINAVEPLLLQNKVDLVLFGHVHN 483
+KDLASV+R+ TPWL+ H P Y++ + + +E LL + +VD+V GHVH
Sbjct: 12 EKDLASVDREVTPWLVATWHPPWYSTYKAHYKEAECMRVEMEDLLYKYRVDIVFNGHVHA 191
Query: 484 YERTCSVY 491
YER+ VY
Sbjct: 192 YERSNRVY 215
>AW719640
Length = 516
Score = 54.7 bits (130), Expect = 4e-08
Identities = 21/42 (50%), Positives = 27/42 (64%)
Frame = +2
Query: 208 TFSQEDMCSDPAKDFGWHDPGYIHSALMTGLKPSSAYSYRYG 249
TF + MC PA GW DPG+IH++ + L P+S YSYR G
Sbjct: 386 TFGRSSMCGSPASTVGWRDPGFIHTSFLKNLWPNSVYSYRLG 511
>TC18698
Length = 808
Score = 45.1 bits (105), Expect = 3e-05
Identities = 24/62 (38%), Positives = 29/62 (46%)
Frame = +2
Query: 481 VHNYERTCSVYQNKCKAIPIKDQKGVDTYDNRNYSAPVHAVIGMAGFALDKFSNNVTNFP 540
VHNYERTC VYQNKC G+ + +H V G AG L +FS +
Sbjct: 2 VHNYERTCPVYQNKCVNTEKSHYSGI-------VNGTIHVVAGGAGSHLSRFSEKTPEWS 160
Query: 541 FV 542
V
Sbjct: 161 LV 166
>TC16271 similar to GB|AAM15909.1|20257479|AF492660 purple acid phosphatase
{Arabidopsis thaliana;} , partial (32%)
Length = 653
Score = 33.1 bits (74), Expect = 0.13
Identities = 17/53 (32%), Positives = 28/53 (52%)
Frame = +1
Query: 437 WLIFMGHRPMYTSNNGFSSKDQKFINAVEPLLLQNKVDLVLFGHVHNYERTCS 489
W I +GH P+ + + +K+ I + P+L +N VD+ + GH H E S
Sbjct: 76 WKIVVGHHPIRSIGHHGDTKE--LIRQLLPILEENNVDMYINGHDHCLEHITS 228
>AV416789
Length = 163
Score = 33.1 bits (74), Expect = 0.13
Identities = 20/41 (48%), Positives = 21/41 (50%), Gaps = 6/41 (14%)
Frame = +2
Query: 352 IGNHERDYI------DSGSVYVTPDSGGECGVPYETYFPMP 386
IGNHE D+ D S D GGECGVPY F MP
Sbjct: 11 IGNHEYDWPLQPWKPDWASY--GKDGGGECGVPYSLRFNMP 127
>BP038458
Length = 427
Score = 32.7 bits (73), Expect = 0.17
Identities = 20/59 (33%), Positives = 27/59 (44%)
Frame = -1
Query: 282 KTPLDASEEHYIQPGALSVIKAIANEVNSNNVNSVFHIGDISYATGFLAEWDFFLNLIS 340
+T + +HYI P LS +A NS IGD + + GFL+ W LIS
Sbjct: 379 ETIMQMKPKHYIHPPLLSTFSLVA-------ANSSCWIGDTNRSIGFLSFWGATFGLIS 224
>TC9054
Length = 697
Score = 30.8 bits (68), Expect = 0.64
Identities = 26/80 (32%), Positives = 40/80 (49%), Gaps = 10/80 (12%)
Frame = +3
Query: 9 KHVFILLFFFLSNPSMSSTFPSSTLDL---TVTHTKAL-HQNFTALSDFRLINRRIL--- 61
+H F LLF PS+S TF S+T L T+ A+ +Q+ T + + + NRRI+
Sbjct: 12 EHNFSLLFSLKKTPSLSLTFISNTQTLFQTTLFFNMAIENQDNTTVREIKPKNRRIMGAG 191
Query: 62 ---NDCSHLSPYLKLNITSN 78
D + P+LK + N
Sbjct: 192GPEEDDNRWPPWLKPLLKEN 251
>TC10120 similar to UP|Q9LTA2 (Q9LTA2) Similarity to AT-hook DNA-binding
protein, partial (40%)
Length = 847
Score = 30.0 bits (66), Expect = 1.1
Identities = 11/27 (40%), Positives = 15/27 (54%)
Frame = -1
Query: 369 PDSGGECGVPYETYFPMPTAAKDKPWY 395
PD+GG+C P E FP ++ WY
Sbjct: 424 PDTGGDCTSPAEAEFPASSSGGGSLWY 344
>TC20218
Length = 559
Score = 29.3 bits (64), Expect = 1.9
Identities = 31/126 (24%), Positives = 49/126 (38%), Gaps = 1/126 (0%)
Frame = +2
Query: 204 NIVTTFSQEDMCSDPAKDFGWHDPGYIHSALMTGLKPSSAYSYRYGSNSADWSEQTKFST 263
N + T E +C DP H A T + ++ Y Y S S++ F +
Sbjct: 140 NGLATQGIESICMDPQ-----------HGAEATKQEVANFNGYNYLSMCELPSQKGSFMS 286
Query: 264 PPAGGSDELKFISFGDMGKTPLDASE-EHYIQPGALSVIKAIANEVNSNNVNSVFHIGDI 322
P S FGDM K S + +Q LS + E +++N +F+ +
Sbjct: 287 PSNSCSSNFNTPQFGDMDKVSSCVSMLKGTLQRRRLS--SQLEKEAAEDDLNGIFYPQEP 460
Query: 323 SYATGF 328
+ TGF
Sbjct: 461 LFQTGF 478
>TC14503
Length = 1110
Score = 28.5 bits (62), Expect = 3.2
Identities = 21/74 (28%), Positives = 32/74 (42%), Gaps = 5/74 (6%)
Frame = -2
Query: 50 LSDFRLINRRILNDCSHLSPYLKLNITSNSKLLDEEFVTVTV-----TGVSKPRDGDWVA 104
++ FR+ + L S LSP + SNS L + TV T R D+ +
Sbjct: 335 VTKFRIFHHHHLFSFSLLSPLSNVKSPSNSNLTQSTILMCTVQTSAGTKKEMSR*RDFSS 156
Query: 105 MISPSNSKLQNFLY 118
++ NSK QN +
Sbjct: 155 IVIHENSKTQNLFF 114
>CN825496
Length = 616
Score = 28.5 bits (62), Expect = 3.2
Identities = 17/58 (29%), Positives = 25/58 (42%), Gaps = 8/58 (13%)
Frame = +1
Query: 364 SVYVTPDSGGECGVPYET--------YFPMPTAAKDKPWYSIEQGSVHFTVISTEHDW 413
++ V DS GVP T F P ++PWY IEQ +T++ + W
Sbjct: 244 NILVMCDSYTPSGVPLPTNKRHAAAKIFSHPDVVAEEPWYGIEQ---EYTLLQKDIQW 408
>CB827350
Length = 517
Score = 28.1 bits (61), Expect = 4.1
Identities = 14/46 (30%), Positives = 23/46 (49%)
Frame = +2
Query: 3 SSNWVLKHVFILLFFFLSNPSMSSTFPSSTLDLTVTHTKALHQNFT 48
S+N L H+ L+FFF + + P S + + + +H NFT
Sbjct: 101 SNNQYLSHLLFLIFFFYFSFDLECIPP*SCICMPIFILILIHSNFT 238
>TC12473 similar to UP|Q9M068 (Q9M068) Protein kinase-like protein, partial
(42%)
Length = 946
Score = 27.7 bits (60), Expect = 5.4
Identities = 13/35 (37%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = +2
Query: 120 AITLSRLHELQKERMQERTEWEMQCDNL-*WFNKV 153
A+ H + + +Q T WE+QC N WFNK+
Sbjct: 824 ALLXENSHIQRFQGLQYTT*WELQCKNFXFWFNKI 928
>TC17166 homologue to UP|Q84L15 (Q84L15) Cytosolic glutamine synthetase,
partial (49%)
Length = 597
Score = 27.7 bits (60), Expect = 5.4
Identities = 11/31 (35%), Positives = 17/31 (54%)
Frame = +3
Query: 383 FPMPTAAKDKPWYSIEQGSVHFTVISTEHDW 413
F P A + PWY IEQ +T++ + +W
Sbjct: 411 FSQPEVAAEVPWYGIEQ---EYTLLQKDINW 494
>TC12483 weakly similar to UP|Q40552 (Q40552) Pistil extensin like protein
(Fragment), partial (6%)
Length = 534
Score = 27.7 bits (60), Expect = 5.4
Identities = 14/33 (42%), Positives = 21/33 (63%), Gaps = 5/33 (15%)
Frame = +2
Query: 15 LFFFLSNPSMSSTF-----PSSTLDLTVTHTKA 42
LFFFLS+PS + F PS++ +++ HT A
Sbjct: 20 LFFFLSSPSPKTDFFIL*SPSASSSISLPHTAA 118
>AV417488
Length = 289
Score = 27.3 bits (59), Expect = 7.1
Identities = 12/24 (50%), Positives = 17/24 (70%)
Frame = -2
Query: 24 MSSTFPSSTLDLTVTHTKALHQNF 47
+S +PSST DL V+ +LHQ+F
Sbjct: 288 LSPLYPSSTSDLHVSIATSLHQSF 217
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.325 0.138 0.444
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,524,959
Number of Sequences: 28460
Number of extensions: 176627
Number of successful extensions: 1227
Number of sequences better than 10.0: 44
Number of HSP's better than 10.0 without gapping: 1215
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1222
length of query: 586
length of database: 4,897,600
effective HSP length: 95
effective length of query: 491
effective length of database: 2,193,900
effective search space: 1077204900
effective search space used: 1077204900
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 58 (26.9 bits)
Medicago: description of AC146631.6