BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146590.2 + phase: 0
(313 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV767342 185 1e-47
TC14408 weakly similar to UP|Q8RWR7 (Q8RWR7) Anther-specific pro... 163 7e-42
TC19826 similar to UP|Q43680 (Q43680) Mung bean seed albumin, pa... 157 3e-39
AV767504 100 4e-22
BP038854 92 1e-19
AV419417 41 3e-04
BP042122 27 3.4
TC19523 27 4.5
TC17703 homologue to UP|CAC19183 (CAC19183) Expansin, partial (82%) 27 4.5
TC18556 similar to UP|Q9XIN0 (Q9XIN0) At2g27230 protein (BHLH tr... 27 5.8
TC10318 similar to UP|Q9LP91 (Q9LP91) T32E20.29, partial (53%) 26 7.6
TC15474 UP|Q7Y1B9 (Q7Y1B9) Ammonium transporter, complete 26 9.9
>AV767342
Length = 490
Score = 185 bits (469), Expect = 1e-47
Identities = 91/157 (57%), Positives = 119/157 (74%), Gaps = 1/157 (0%)
Frame = +1
Query: 63 PIYINAAFRASANNEAYLFMNNEYVLINYARGSTNDYIINGPLYICDGYPSLARTPFGEH 122
P +NAAFR+S NNEAY+F+NNEY+L +YA GS++D I+ GPL I DG+PSL TPFG+H
Sbjct: 16 PGSVNAAFRSSKNNEAYIFINNEYILEDYAPGSSHDKILKGPLLISDGFPSLKGTPFGDH 195
Query: 123 GIDCAFDTDKTQAYIFSANLCALIDYA-PGTTNDKILSGPMKITDMFPFFKDTVFERGLD 181
GI+CAFDTD T A+IFS LCAL+DYA PG + SGP I +MFP K T FE+ ++
Sbjct: 196 GIECAFDTDGTDAFIFSGELCALVDYAKPGDEYMMLESGP--IYEMFPRLKGTAFEKRIN 369
Query: 182 AAFRAHSSNEAYLFRGGHYALINYSSKRLIHIETIRH 218
AAFR+ SNEAY+F G YA INY+SK++I+ + I++
Sbjct: 370 AAFRSSLSNEAYIFTGDQYARINYASKKVIYTDAIKN 480
Score = 97.8 bits (242), Expect = 2e-21
Identities = 59/148 (39%), Positives = 85/148 (56%), Gaps = 6/148 (4%)
Frame = +1
Query: 117 TPFGEHGIDCAFDTDKT-QAYIFSANLCALIDYAPGTTNDKILSGPMKITDMFPFFKDTV 175
+P ++ AF + K +AYIF N L DYAPG+++DKIL GP+ I+D FP K T
Sbjct: 4 SPIAPGSVNAAFRSSKNNEAYIFINNEYILEDYAPGSSHDKILKGPLLISDGFPSLKGTP 183
Query: 176 F-ERGLDAAFRAHSSNEAYLFRGGHYALINYSSK----RLIHIETIRHGFPSLIGTVFEN 230
F + G++ AF + +A++F G AL++Y+ ++ I FP L GT FE
Sbjct: 184 FGDHGIECAFDTDGT-DAFIFSGELCALVDYAKPGDEYMMLESGPIYEMFPRLKGTAFEK 360
Query: 231 GLEAAFASHGTKEAYLFKGEYYANIYYA 258
+ AAF S + EAY+F G+ YA I YA
Sbjct: 361 RINAAFRSSLSNEAYIFTGDQYARINYA 444
Score = 37.4 bits (85), Expect = 0.003
Identities = 22/51 (43%), Positives = 29/51 (56%)
Frame = +1
Query: 232 LEAAFASHGTKEAYLFKGEYYANIYYAPGSTDDYLIGGRVKLILSNWPSLK 282
+ AAF S EAY+F Y YAPGS+ D ++ G + LI +PSLK
Sbjct: 25 VNAAFRSSKNNEAYIFINNEYILEDYAPGSSHDKILKGPL-LISDGFPSLK 174
>TC14408 weakly similar to UP|Q8RWR7 (Q8RWR7) Anther-specific protein,
partial (49%)
Length = 1040
Score = 163 bits (412), Expect(2) = 7e-42
Identities = 97/199 (48%), Positives = 125/199 (62%), Gaps = 4/199 (2%)
Frame = +2
Query: 95 STNDYIINGPLYICDGYPSLAR-TPFGEH-GIDCAFDTDKTQAYIFSAN-LCALID-YAP 150
+T+D +NGP I R TP E+ + CAF FS +CA I
Sbjct: 65 TTDDTTLNGPYPISAALHVTVRGTPV*ENMAVKCAFGRWIATRPTFSPETMCAYIY*VLH 244
Query: 151 GTTNDKILSGPMKITDMFPFFKDTVFERGLDAAFRAHSSNEAYLFRGGHYALINYSSKRL 210
+++DKIL GPM I DMFP K TVFE+ +DAAFR+ SNEAY+F G YA INY+S +
Sbjct: 245 ISSDDKILRGPMSIPDMFPCLKGTVFEKRIDAAFRSSVSNEAYIFTGDQYARINYASISV 424
Query: 211 IHIETIRHGFPSLIGTVFENGLEAAFASHGTKEAYLFKGEYYANIYYAPGSTDDYLIGGR 270
+ +TI++ +P L GT+FE+G+EAAFASH T EAYLFKGE YA I + PG +DYL+GG
Sbjct: 425 SYPKTIKNEWPCLAGTIFESGIEAAFASHITNEAYLFKGEQYARIKFTPGKNEDYLMGG- 601
Query: 271 VKLILSNWPSLKKILPRNN 289
V+ ILS WP L +L N
Sbjct: 602 VRPILSYWPGLGGLLASKN 658
Score = 64.7 bits (156), Expect = 2e-11
Identities = 41/105 (39%), Positives = 59/105 (56%), Gaps = 1/105 (0%)
Frame = +2
Query: 66 INAAFRASANNEAYLFMNNEYVLINYARGSTNDYIINGPLYICDGYPSLARTPFGEHGID 125
I+AAFR+S +NEAY+F ++Y INYA S ++ P I + +P LA T F E GI+
Sbjct: 332 IDAAFRSSVSNEAYIFTGDQYARINYASIS-----VSYPKTIKNEWPCLAGTIF-ESGIE 493
Query: 126 CAFDTDKT-QAYIFSANLCALIDYAPGTTNDKILSGPMKITDMFP 169
AF + T +AY+F A I + PG D ++ G I +P
Sbjct: 494 AAFASHITNEAYLFKGEQYARIKFTPGKNEDYLMGGVRPILSYWP 628
Score = 40.4 bits (93), Expect = 4e-04
Identities = 19/49 (38%), Positives = 25/49 (50%)
Frame = +2
Query: 66 INAAFRASANNEAYLFMNNEYVLINYARGSTNDYIINGPLYICDGYPSL 114
I AAF + NEAYLF +Y I + G DY++ G I +P L
Sbjct: 488 IEAAFASHITNEAYLFKGEQYARIKFTPGKNEDYLMGGVRPILSYWPGL 634
Score = 28.1 bits (61), Expect = 2.0
Identities = 22/70 (31%), Positives = 34/70 (48%), Gaps = 4/70 (5%)
Frame = +1
Query: 85 EYVLINYARGST--NDYIINGPLYICDGYPSLARTPFGEHGIDCAFDT-DKTQAYIFSAN 141
+Y+ ++YA G+ +++ + C T GEHG T D+ +AYIFS N
Sbjct: 34 QYISLDYAPGNH**HNFEWAISYFCCFACHCKRHTSVGEHGSKMRLWTLDRNEAYIFSGN 213
Query: 142 -LCALIDYAP 150
+C I AP
Sbjct: 214 HVCLHILSAP 243
Score = 23.9 bits (50), Expect(2) = 7e-42
Identities = 10/14 (71%), Positives = 11/14 (78%)
Frame = +3
Query: 76 NEAYLFMNNEYVLI 89
NEAY+FMNN V I
Sbjct: 3 NEAYIFMNNVSVYI 44
>TC19826 similar to UP|Q43680 (Q43680) Mung bean seed albumin, partial (21%)
Length = 413
Score = 157 bits (396), Expect = 3e-39
Identities = 76/120 (63%), Positives = 94/120 (78%), Gaps = 3/120 (2%)
Frame = +3
Query: 63 PIYINAAFRASANNEAYLFMNNEYVLINYARGSTNDYIINGP--LYICDGYPSLARTPFG 120
P+ INAAFR+S +N AY+F+N+EYVL++Y G+T D ++NG + I DG+PSL TPFG
Sbjct: 54 PVSINAAFRSSRHNAAYIFINDEYVLLDYGPGTTTDKVLNGGRVIRISDGFPSLKGTPFG 233
Query: 121 EHGIDCAFDTDKTQAYIFSANLCALIDYAPGTTND-KILSGPMKITDMFPFFKDTVFERG 179
EHGIDCAFDT+ +AYIFS +CA IDYAPGTT D KIL GP+KI DMFP K TVFE+G
Sbjct: 234 EHGIDCAFDTNGDKAYIFSGEMCAYIDYAPGTTGDEKILKGPLKIGDMFPCLKCTVFEKG 413
Score = 65.9 bits (159), Expect = 9e-12
Identities = 41/103 (39%), Positives = 66/103 (63%), Gaps = 9/103 (8%)
Frame = +3
Query: 180 LDAAFRAHSSNEAYLFRGGHYALINY----SSKRLIH---IETIRHGFPSLIGTVF-ENG 231
++AAFR+ N AY+F Y L++Y ++ ++++ + I GFPSL GT F E+G
Sbjct: 63 INAAFRSSRHNAAYIFINDEYVLLDYGPGTTTDKVLNGGRVIRISDGFPSLKGTPFGEHG 242
Query: 232 LEAAFASHGTKEAYLFKGEYYANIYYAPGST-DDYLIGGRVKL 273
++ AF ++G K AY+F GE A I YAPG+T D+ ++ G +K+
Sbjct: 243 IDCAFDTNGDK-AYIFSGEMCAYIDYAPGTTGDEKILKGPLKI 368
Score = 39.7 bits (91), Expect = 7e-04
Identities = 22/52 (42%), Positives = 30/52 (57%), Gaps = 1/52 (1%)
Frame = +3
Query: 232 LEAAFASHGTKEAYLFKGEYYANIYYAPGSTDDYLI-GGRVKLILSNWPSLK 282
+ AAF S AY+F + Y + Y PG+T D ++ GGRV I +PSLK
Sbjct: 63 INAAFRSSRHNAAYIFINDEYVLLDYGPGTTTDKVLNGGRVIRISDGFPSLK 218
>AV767504
Length = 579
Score = 100 bits (248), Expect = 4e-22
Identities = 51/103 (49%), Positives = 71/103 (68%), Gaps = 1/103 (0%)
Frame = -1
Query: 189 SNEAYLFRGGHYALINYSSKRLIHIETIRHGFPSLIGTVFENGLEAAFASHGTKEAYLFK 248
S++AY+F+GG YA ++Y S L + TIRHG+ SL GT+FE+G+EAAFA+H AY+FK
Sbjct: 579 SSDAYIFKGGQYAHLDYGSNEL-YTNTIRHGWRSLAGTIFESGVEAAFATHVPNRAYVFK 403
Query: 249 GEYYANIYYAPGSTD-DYLIGGRVKLILSNWPSLKKILPRNNG 290
G+ YA I + P ++D DY+I LI NWP + +L R NG
Sbjct: 402 GDQYALINFIPNTSDGDYMINH--GLISQNWPGIGGVLARKNG 280
Score = 47.4 bits (111), Expect = 3e-06
Identities = 31/90 (34%), Positives = 43/90 (47%)
Frame = -1
Query: 133 TQAYIFSANLCALIDYAPGTTNDKILSGPMKITDMFPFFKDTVFERGLDAAFRAHSSNEA 192
+ AYIF A +DY +N+ + I + T+FE G++AAF H N A
Sbjct: 576 SDAYIFKGGQYAHLDYG---SNELYTN---TIRHGWRSLAGTIFESGVEAAFATHVPNRA 415
Query: 193 YLFRGGHYALINYSSKRLIHIETIRHGFPS 222
Y+F+G YALIN+ I HG S
Sbjct: 414 YVFKGDQYALINFIPNTSDGDYMINHGLIS 325
Score = 40.8 bits (94), Expect = 3e-04
Identities = 29/96 (30%), Positives = 51/96 (52%), Gaps = 1/96 (1%)
Frame = -1
Query: 75 NNEAYLFMNNEYVLINYARGSTNDYIINGPLYICDGYPSLARTPFGEHGIDCAFDTD-KT 133
+++AY+F +Y ++Y +N+ N I G+ SLA T F E G++ AF T
Sbjct: 579 SSDAYIFKGGQYAHLDYG---SNELYTNT---IRHGWRSLAGTIF-ESGVEAAFATHVPN 421
Query: 134 QAYIFSANLCALIDYAPGTTNDKILSGPMKITDMFP 169
+AY+F + ALI++ P T++ + I+ +P
Sbjct: 420 RAYVFKGDQYALINFIPNTSDGDYMINHGLISQNWP 313
Score = 34.3 bits (77), Expect = 0.028
Identities = 19/50 (38%), Positives = 27/50 (54%), Gaps = 1/50 (2%)
Frame = -1
Query: 66 INAAFRASANNEAYLFMNNEYVLINY-ARGSTNDYIINGPLYICDGYPSL 114
+ AAF N AY+F ++Y LIN+ S DY+IN L I +P +
Sbjct: 453 VEAAFATHVPNRAYVFKGDQYALINFIPNTSDGDYMINHGL-ISQNWPGI 307
>BP038854
Length = 450
Score = 92.0 bits (227), Expect = 1e-19
Identities = 45/85 (52%), Positives = 62/85 (72%)
Frame = -1
Query: 180 LDAAFRAHSSNEAYLFRGGHYALINYSSKRLIHIETIRHGFPSLIGTVFENGLEAAFASH 239
+DAAFR+ S+EAY+F G YA INY+S + + +T+ + +P L GT+FE+G+EAAFASH
Sbjct: 444 IDAAFRSSVSHEAYIFTGDPYARINYASISVSYPQTL*NEWPCLAGTIFESGVEAAFASH 265
Query: 240 GTKEAYLFKGEYYANIYYAPGSTDD 264
T EAYLFKGE YA I + G ++
Sbjct: 264 ITHEAYLFKGEPYARIKFTSGQNEE 190
Score = 55.8 bits (133), Expect = 9e-09
Identities = 33/85 (38%), Positives = 53/85 (61%), Gaps = 1/85 (1%)
Frame = -1
Query: 124 IDCAFDTDKT-QAYIFSANLCALIDYAPGTTNDKILSGPMKITDMFPFFKDTVFERGLDA 182
ID AF + + +AYIF+ + A I+YA + +S P + + +P T+FE G++A
Sbjct: 444 IDAAFRSSVSHEAYIFTGDPYARINYASIS-----VSYPQTL*NEWPCLAGTIFESGVEA 280
Query: 183 AFRAHSSNEAYLFRGGHYALINYSS 207
AF +H ++EAYLF+G YA I ++S
Sbjct: 279 AFASHITHEAYLFKGEPYARIKFTS 205
Score = 49.7 bits (117), Expect = 6e-07
Identities = 33/91 (36%), Positives = 51/91 (55%), Gaps = 1/91 (1%)
Frame = -1
Query: 66 INAAFRASANNEAYLFMNNEYVLINYARGSTNDYIINGPLYICDGYPSLARTPFGEHGID 125
I+AAFR+S ++EAY+F + Y INYA S ++ P + + +P LA T F E G++
Sbjct: 444 IDAAFRSSVSHEAYIFTGDPYARINYASIS-----VSYPQTL*NEWPCLAGTIF-ESGVE 283
Query: 126 CAFDTDKT-QAYIFSANLCALIDYAPGTTND 155
AF + T +AY+F A I + G +
Sbjct: 282 AAFASHITHEAYLFKGEPYARIKFTSGQNEE 190
>AV419417
Length = 405
Score = 40.8 bits (94), Expect = 3e-04
Identities = 19/43 (44%), Positives = 26/43 (60%)
Frame = -2
Query: 247 FKGEYYANIYYAPGSTDDYLIGGRVKLILSNWPSLKKILPRNN 289
FKG+ YA I + PG +DY I +VK I +W +L +L R N
Sbjct: 401 FKGDQYARIKFTPGKNEDYDIIDKVKPIKPSWNALGDLLERKN 273
>BP042122
Length = 505
Score = 27.3 bits (59), Expect = 3.4
Identities = 11/38 (28%), Positives = 20/38 (51%)
Frame = -1
Query: 276 SNWPSLKKILPRNNGRLDLHTHKDHPDEDNGSYDHIEL 313
++WP++K+ + N H H P E +G+ D + L
Sbjct: 487 ADWPTVKRYISCLNDTEGEHVHPHGPSEGDGNMDPMNL 374
>TC19523
Length = 524
Score = 26.9 bits (58), Expect = 4.5
Identities = 10/23 (43%), Positives = 17/23 (73%)
Frame = -2
Query: 43 SSSQDNEEVELHSYGAVENIPIY 65
+++++NE++ELHSY N P Y
Sbjct: 436 NATKNNEKLELHSYSPFTN*PTY 368
>TC17703 homologue to UP|CAC19183 (CAC19183) Expansin, partial (82%)
Length = 687
Score = 26.9 bits (58), Expect = 4.5
Identities = 11/23 (47%), Positives = 12/23 (51%)
Frame = -3
Query: 288 NNGRLDLHTHKDHPDEDNGSYDH 310
N G LD HTHK HP + H
Sbjct: 175 NLG*LDFHTHKPHPLSQKHHFHH 107
>TC18556 similar to UP|Q9XIN0 (Q9XIN0) At2g27230 protein (BHLH transcription
factor), partial (24%)
Length = 710
Score = 26.6 bits (57), Expect = 5.8
Identities = 9/29 (31%), Positives = 16/29 (55%)
Frame = -3
Query: 202 LINYSSKRLIHIETIRHGFPSLIGTVFEN 230
++ S ++H +IRH FP L T+ +
Sbjct: 348 MVRSRSASMLHFASIRHNFPQLFHTILNH 262
>TC10318 similar to UP|Q9LP91 (Q9LP91) T32E20.29, partial (53%)
Length = 910
Score = 26.2 bits (56), Expect = 7.6
Identities = 11/23 (47%), Positives = 16/23 (68%)
Frame = -2
Query: 84 NEYVLINYARGSTNDYIINGPLY 106
+ +VLIN RGS+N Y+ N L+
Sbjct: 891 SHWVLINRIRGSSNQYLHNWTLF 823
>TC15474 UP|Q7Y1B9 (Q7Y1B9) Ammonium transporter, complete
Length = 1950
Score = 25.8 bits (55), Expect = 9.9
Identities = 12/27 (44%), Positives = 16/27 (58%)
Frame = +3
Query: 248 KGEYYANIYYAPGSTDDYLIGGRVKLI 274
KGEY A +Y + G L+GG KL+
Sbjct: 1251 KGEYVAEVYGSAGRPYGLLMGGGGKLL 1331
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.319 0.139 0.418
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,141,080
Number of Sequences: 28460
Number of extensions: 68248
Number of successful extensions: 319
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 287
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 302
length of query: 313
length of database: 4,897,600
effective HSP length: 90
effective length of query: 223
effective length of database: 2,336,200
effective search space: 520972600
effective search space used: 520972600
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)
Medicago: description of AC146590.2