BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146590.14 - phase: 1 /pseudo
(299 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BG662253 193 3e-50
TC14411 similar to UP|O65848 (O65848) Annexin, complete 138 1e-33
TC9761 similar to UP|O65848 (O65848) Annexin, partial (55%) 106 5e-24
TC9306 similar to UP|Q42922 (Q42922) Annexin (Fragment), partial... 102 1e-22
TC16388 similar to UP|O82090 (O82090) Fiber annexin, partial (48%) 92 1e-19
TC13742 similar to UP|Q9ZR53 (Q9ZR53) Annexin-like protein, part... 66 8e-12
TC9709 similar to PIR|S56674|S56674 annexin homolog RJ4 (clone R... 65 2e-11
TC15575 similar to UP|O82090 (O82090) Fiber annexin, partial (45%) 62 2e-10
BF177689 59 1e-09
TC16151 similar to UP|Q9ZR53 (Q9ZR53) Annexin-like protein, part... 59 1e-09
BP073675 52 9e-08
TC10305 homologue to UP|Q42922 (Q42922) Annexin (Fragment), part... 50 4e-07
CB829084 37 0.004
BF177679 26 7.1
TC12601 similar to UP|Q9LTX6 (Q9LTX6) Genomic DNA, chromosome 5,... 26 7.1
TC15604 similar to UP|PROC_PEA (Q04708) Pyrroline-5-carboxylate ... 26 7.1
>BG662253
Length = 342
Score = 193 bits (490), Expect = 3e-50
Identities = 96/108 (88%), Positives = 102/108 (93%)
Frame = +2
Query: 65 SMHPWERDARLAKEALKKGSISYGVLIEIACTRSSEELLGARKAYHSLFDHSIEEDVASH 124
+MHPWERDARL KEALKKG +YGVLIEIACTRSSEELLGARKAYHSLFDHSIEEDVASH
Sbjct: 17 TMHPWERDARLVKEALKKGPNAYGVLIEIACTRSSEELLGARKAYHSLFDHSIEEDVASH 196
Query: 125 IHGNDRKLLVALVSAYRYEGTKVKDDTAKSEAKTLSNAIKNAQNKPIV 172
IHG +RKLLVALVSAYRYE +KVKDDTAKSEAKT+SNAIKNAQ KPI+
Sbjct: 197 IHGIERKLLVALVSAYRYERSKVKDDTAKSEAKTISNAIKNAQKKPII 340
>TC14411 similar to UP|O65848 (O65848) Annexin, complete
Length = 1306
Score = 138 bits (348), Expect = 1e-33
Identities = 102/308 (33%), Positives = 159/308 (51%), Gaps = 11/308 (3%)
Frame = +3
Query: 2 GVDEKSLIAVLGKWDPLERETYRKKTSHFFIEDHERQFQRWNDHCVRLLKHEFVR-FKNA 60
G DE +I++LG + +R+ R+ + ED V+ L+ E + A
Sbjct: 126 GTDENLVISILGHRNVHQRQAIRRAYEEIYQED-----------LVKRLESEIKGDLEKA 272
Query: 61 VVLWSMHPWERDARLAKEALKKGSISYGVLIEIACTRSSEELLGARKAYHSLFDHSIEED 120
V W++ +RDA L +K G +Y V++EI+ S EELL R+AY + + HS+EED
Sbjct: 273 VYRWNLEHADRDAVLINVVIKSGK-NYHVIVEISSVLSPEELLAVRRAYLNRYKHSLEED 449
Query: 121 VASHIHGNDRKLLVALVSAYRYEGTKVKDDTAKSEAKTLSNAIKNAQNKPIVEDDEVIRI 180
VA+H G+ R+LLV LV+++RY G ++ A+SEA+ L A+K + +E IRI
Sbjct: 450 VAAHTSGHLRQLLVGLVTSFRYVGEEINAKLAQSEAEILHEAVKEKKG----SHEEAIRI 617
Query: 181 LATRSKLXLQAVYTAL*RDFWEES*RGIINK*TLFKET----------VQCLCTPQVYFS 230
L TRSK L A + + E I K L +++ ++C Y+
Sbjct: 618 LTTRSKTQLIATFNR-----YREIHGTSITKKLLDEKSDDFQKGLYTAIRCFNDHIKYYE 782
Query: 231 KVLDAALKNDVNKNIKKSLTRVIVTRADIDMKEIKAEYNNLYGVSLPQKIEETAKGNYKD 290
KV+ A+K + + +LTRVIV+RA+ D+K I Y V L + + G+YK
Sbjct: 783 KVVRDAIKK--SGTDEDALTRVIVSRAEKDLKLISDVYYKRNSVHLEDAVAKEISGDYKK 956
Query: 291 FLLTLIAR 298
FLLTL+ +
Sbjct: 957 FLLTLLGK 980
>TC9761 similar to UP|O65848 (O65848) Annexin, partial (55%)
Length = 558
Score = 106 bits (264), Expect = 5e-24
Identities = 62/159 (38%), Positives = 90/159 (55%), Gaps = 1/159 (0%)
Frame = +1
Query: 2 GVDEKSLIAVLGKWDPLERETYRKKTSHFFIEDHERQFQRWNDHCVRLLKHEFVR-FKNA 60
G DEK++IA+LG +R+ RK + ED ++ L+ E + A
Sbjct: 115 GTDEKTVIAILGHRSVHQRQQIRKAYEELYQED-----------IIKRLESELSGDIEKA 261
Query: 61 VVLWSMHPWERDARLAKEALKKGSISYGVLIEIACTRSSEELLGARKAYHSLFDHSIEED 120
V W + P +RDA LA A+K G Y V++EIA S EE+L R+AYH+ + S+EED
Sbjct: 262 VYRWMLEPTDRDAVLANVAIKSGGKGYNVIVEIATVLSPEEVLAVRRAYHNRYKRSLEED 441
Query: 121 VASHIHGNDRKLLVALVSAYRYEGTKVKDDTAKSEAKTL 159
VA+H G+ R+L V LVS++RY G ++ AK+EA L
Sbjct: 442 VAAHTTGDLRQLWVGLVSSFRYGGDEINARLAKTEADIL 558
>TC9306 similar to UP|Q42922 (Q42922) Annexin (Fragment), partial (59%)
Length = 662
Score = 102 bits (253), Expect = 1e-22
Identities = 66/163 (40%), Positives = 92/163 (55%), Gaps = 1/163 (0%)
Frame = +1
Query: 2 GVDEKSLIAVLGKWDPLERETYRKKTSHFFIEDHERQFQRWNDHCVRLLKHEFVR-FKNA 60
G +E +I++L + +R+ HE Q + + + L E F+ A
Sbjct: 175 GTNEDLIISILAHRNAAQRKLI-----------HETYSQTYGEDLLTDLDKELSSDFERA 321
Query: 61 VVLWSMHPWERDARLAKEALKKGSISYGVLIEIACTRSSEELLGARKAYHSLFDHSIEED 120
VVLW++ P ERDA L EA K+ + + +L+EIA TRSS +L A++AY + F SIEED
Sbjct: 322 VVLWTLGPAERDAFLVNEATKRLTKNNWILMEIASTRSSLDLFKAKQAYQARFKRSIEED 501
Query: 121 VASHIHGNDRKLLVALVSAYRYEGTKVKDDTAKSEAKTLSNAI 163
VA H G+ RKLLV LV +RY+G +V AKSEAK L I
Sbjct: 502 VAYHTSGDIRKLLVPLVGTFRYDGDEVNMILAKSEAKLLHEKI 630
>TC16388 similar to UP|O82090 (O82090) Fiber annexin, partial (48%)
Length = 574
Score = 92.0 bits (227), Expect = 1e-19
Identities = 55/136 (40%), Positives = 80/136 (58%), Gaps = 1/136 (0%)
Frame = +1
Query: 2 GVDEKSLIAVLGKWDPLERETYRKKTSHFFIEDHERQFQRWNDHCVRLLKHEFVR-FKNA 60
G +E +I++LG + +R+ R+ + + ED ++ L E F+
Sbjct: 199 GTNEDLIISILGHRNAAQRKLIRETYAETYGED-----------LLKALDKELTSDFERL 345
Query: 61 VVLWSMHPWERDARLAKEALKKGSISYGVLIEIACTRSSEELLGARKAYHSLFDHSIEED 120
V LW++ ERDA LA EA K+ + S VL+EIACTRSSE++ RKAYH+L+ S+EED
Sbjct: 346 VHLWALDSAERDAFLANEATKRWTSSNQVLVEIACTRSSEQMFAVRKAYHALYKKSLEED 525
Query: 121 VASHIHGNDRKLLVAL 136
VA H G+ RKLL+ L
Sbjct: 526 VAHHTTGDFRKLLLPL 573
>TC13742 similar to UP|Q9ZR53 (Q9ZR53) Annexin-like protein, partial (53%)
Length = 612
Score = 65.9 bits (159), Expect = 8e-12
Identities = 42/140 (30%), Positives = 69/140 (49%)
Frame = +2
Query: 23 YRKKTSHFFIEDHERQFQRWNDHCVRLLKHEFVRFKNAVVLWSMHPWERDARLAKEALKK 82
+R T +I+ R D RL + + AV+LW P RDA + K++L
Sbjct: 194 HRDATQRAYIQQEYRTMYS-GDLLKRLSSELSGKLETAVLLWMHDPAGRDAIILKQSLTV 370
Query: 83 GSISYGVLIEIACTRSSEELLGARKAYHSLFDHSIEEDVASHIHGNDRKLLVALVSAYRY 142
+ E+ C+R+ +L R+ YHS F +E D+ + G+ +K+L+A VS R+
Sbjct: 371 AK-NLETATEVICSRTPSQLQYLRQIYHSKFGTYLEHDIERNTSGDHKKILLAYVSTPRH 547
Query: 143 EGTKVKDDTAKSEAKTLSNA 162
EG +V + A+ +A L A
Sbjct: 548 EGPEVNREMAQKDANVLYKA 607
>TC9709 similar to PIR|S56674|S56674 annexin homolog RJ4 (clone RJ4) -
garden strawberry (fragment) {Fragaria x
ananassa;}, partial (48%)
Length = 577
Score = 64.7 bits (156), Expect = 2e-11
Identities = 44/130 (33%), Positives = 67/130 (50%), Gaps = 5/130 (3%)
Frame = +2
Query: 174 DDEVIRILATRSKLXLQAVYTAL*RDFWEES*RGIINK*T-----LFKETVQCLCTPQVY 228
++E IRIL TRSK L A + D + +++ + ++C+ Q Y
Sbjct: 2 NEEAIRILTTRSKTQLVATFNRYRDDHGISITKKLLDNASDDFHKALHTAIRCINDHQKY 181
Query: 229 FSKVLDAALKNDVNKNIKKSLTRVIVTRADIDMKEIKAEYNNLYGVSLPQKIEETAKGNY 288
+ KVL ALK + + LTRV+VTRA+ D+KEIK Y V L + + G+Y
Sbjct: 182 YEKVLRNALKRVGSD--EDGLTRVVVTRAEKDLKEIKELYYKRNSVHLEDAVAKELSGDY 355
Query: 289 KDFLLTLIAR 298
K F+LTL+ +
Sbjct: 356 KKFILTLLGK 385
>TC15575 similar to UP|O82090 (O82090) Fiber annexin, partial (45%)
Length = 747
Score = 61.6 bits (148), Expect = 2e-10
Identities = 43/133 (32%), Positives = 67/133 (50%), Gaps = 10/133 (7%)
Frame = +1
Query: 174 DDEVIRILATRSKLXLQAVYTAL*RDFWEES*RGIINK*----------TLFKETVQCLC 223
DD++IRILATRS+ + A F ++ INK +L + TV+CL
Sbjct: 40 DDDLIRILATRSRAQINATLNHYKDAFGKD-----INKDLKAEPKDEYLSLLRATVKCLV 204
Query: 224 TPQVYFSKVLDAALKNDVNKNIKKSLTRVIVTRADIDMKEIKAEYNNLYGVSLPQKIEET 283
P+ YF K + ++ + +LTRV+ RA+ID+K I EY + L + I +
Sbjct: 205 RPEKYFEKFIRLSINK--RGTDEGALTRVVAPRAEIDLKIIANEYQRRSSIPLDRAIIKD 378
Query: 284 AKGNYKDFLLTLI 296
G+Y+ LL L+
Sbjct: 379 TNGDYEKMLLALL 417
>BF177689
Length = 509
Score = 58.9 bits (141), Expect = 1e-09
Identities = 40/156 (25%), Positives = 75/156 (47%)
Frame = +3
Query: 2 GVDEKSLIAVLGKWDPLERETYRKKTSHFFIEDHERQFQRWNDHCVRLLKHEFVRFKNAV 61
G D ++I +L D +R +++ + E+ + RL+ + + AV
Sbjct: 72 GCDTTAVINILAHRDATQRAYLQQEYKATYAEELSK----------RLISELSGKLETAV 221
Query: 62 VLWSMHPWERDARLAKEALKKGSISYGVLIEIACTRSSEELLGARKAYHSLFDHSIEEDV 121
+LW P RDA + ++ L +++ E+ C+R+ +L ++ YHS F +E D+
Sbjct: 222 LLWMHDPAGRDATIIRQCLAV-DMNFEGATEVICSRTPSQLQYLKQIYHSKFGVYLEHDI 398
Query: 122 ASHIHGNDRKLLVALVSAYRYEGTKVKDDTAKSEAK 157
+ G+ +K+L+A VS R EG +V + A K
Sbjct: 399 EATTSGDLKKILLAYVSTPRPEGPEVNREIAXKGCK 506
>TC16151 similar to UP|Q9ZR53 (Q9ZR53) Annexin-like protein, partial (31%)
Length = 587
Score = 58.5 bits (140), Expect = 1e-09
Identities = 33/77 (42%), Positives = 44/77 (56%)
Frame = +2
Query: 220 QCLCTPQVYFSKVLDAALKNDVNKNIKKSLTRVIVTRADIDMKEIKAEYNNLYGVSLPQK 279
QC P YF+KVL A+K + +L RVIVTR +IDM+ IKAEY Y +L
Sbjct: 86 QCAVNPAKYFAKVLHKAMKGLGTNDT--TLIRVIVTRTEIDMQYIKAEYLKKYKKTLNDA 259
Query: 280 IEETAKGNYKDFLLTLI 296
+ G+Y+ FLL L+
Sbjct: 260 VHSETSGHYRAFLLALL 310
>BP073675
Length = 402
Score = 52.4 bits (124), Expect = 9e-08
Identities = 30/69 (43%), Positives = 40/69 (57%)
Frame = -3
Query: 228 YFSKVLDAALKNDVNKNIKKSLTRVIVTRADIDMKEIKAEYNNLYGVSLPQKIEETAKGN 287
YF+KVL A+K + K L RVIV R +ID+ IKAEY Y +L + GN
Sbjct: 400 YFAKVLHKAMKGLGTDDTK--LIRVIVIRTEIDLHNIKAEYLKKYKKTLNDAVHSETSGN 227
Query: 288 YKDFLLTLI 296
Y+ FLL+L+
Sbjct: 226 YRAFLLSLL 200
>TC10305 homologue to UP|Q42922 (Q42922) Annexin (Fragment), partial (32%)
Length = 498
Score = 50.4 bits (119), Expect = 4e-07
Identities = 27/83 (32%), Positives = 46/83 (54%)
Frame = +3
Query: 214 LFKETVQCLCTPQVYFSKVLDAALKNDVNKNIKKSLTRVIVTRADIDMKEIKAEYNNLYG 273
L + T++ L P+ YF ++L A+ + +LTRV+ TRA++D+++I EY
Sbjct: 51 LLRATIKSLTYPEKYFEELLRLAINKTGTD--EWALTRVVTTRAEVDLQKIAEEYQKRNS 224
Query: 274 VSLPQKIEETAKGNYKDFLLTLI 296
V L + I G+Y+ LL L+
Sbjct: 225 VPLDRAIANDTSGDYQKILLALM 293
>CB829084
Length = 565
Score = 37.0 bits (84), Expect = 0.004
Identities = 21/55 (38%), Positives = 32/55 (58%)
Frame = +2
Query: 131 KLLVALVSAYRYEGTKVKDDTAKSEAKTLSNAIKNAQNKPIVEDDEVIRILATRS 185
+LLV LV +RY+G +V AKSEAK L I + ++++R++ TRS
Sbjct: 413 QLLVPLVGTFRYDGDEVNMILAKSEAKLLHEKIA----EKAYNHEDLLRVITTRS 565
Score = 26.9 bits (58), Expect = 4.2
Identities = 16/26 (61%), Positives = 17/26 (64%)
Frame = +3
Query: 113 FDHSIEEDVASHIHGNDRKLLVALVS 138
F SIEEDVA H G+ RK VAL S
Sbjct: 6 FKRSIEEDVAYHTSGDIRK--VALTS 77
>BF177679
Length = 331
Score = 26.2 bits (56), Expect = 7.1
Identities = 11/28 (39%), Positives = 16/28 (56%)
Frame = -3
Query: 15 WDPLERETYRKKTSHFFIEDHERQFQRW 42
WD LE YR+KT+H + + Q+W
Sbjct: 155 WDTLEGNIYRRKTTHH--DKVSQMHQQW 78
>TC12601 similar to UP|Q9LTX6 (Q9LTX6) Genomic DNA, chromosome 5, TAC
clone:K9P8, partial (10%)
Length = 524
Score = 26.2 bits (56), Expect = 7.1
Identities = 13/40 (32%), Positives = 22/40 (54%)
Frame = -2
Query: 27 TSHFFIEDHERQFQRWNDHCVRLLKHEFVRFKNAVVLWSM 66
T +F HE+ Q W+D+C+ +L F K ++LW +
Sbjct: 340 TFYFTRAGHEK-LQEWHDNCILVLNQIFT--KAELLLWQV 230
>TC15604 similar to UP|PROC_PEA (Q04708) Pyrroline-5-carboxylate reductase
(P5CR) (P5C reductase) , complete
Length = 1124
Score = 26.2 bits (56), Expect = 7.1
Identities = 16/51 (31%), Positives = 28/51 (54%), Gaps = 2/51 (3%)
Frame = +2
Query: 75 LAKEALKKGSISYGVLIEIACTRSSEELLGARKAYHSLFDH--SIEEDVAS 123
LA EAL G ++ G+ ++A + +S+ +LGA H +++DV S
Sbjct: 551 LAVEALADGGVAAGLPRDLALSLASQTVLGAASMVTPTGKHPGQLKDDVTS 703
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.324 0.138 0.407
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,547,727
Number of Sequences: 28460
Number of extensions: 55160
Number of successful extensions: 345
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 332
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 333
length of query: 299
length of database: 4,897,600
effective HSP length: 90
effective length of query: 209
effective length of database: 2,336,200
effective search space: 488265800
effective search space used: 488265800
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 55 (25.8 bits)
Medicago: description of AC146590.14