TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC146588.9 + phase: 0 
         (373 letters)

Database: LJGI 
           28,460 sequences; 14,692,800 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC8957 weakly similar to UP|Q9ZUJ5 (Q9ZUJ5) T2K10.2 protein, par...   311  1e-85
TC16638 weakly similar to UP|MEP1_SOYBN (P29136) Metalloendoprot...   200  4e-52
TC14335 similar to UP|MEP1_SOYBN (P29136) Metalloendoproteinase ...   166  6e-42
BP078780                                                               88  2e-18
TC12158 homologue to UP|143A_SOYBN (Q96450) 14-3-3-like protein ...    30  0.50
AV417355                                                               27  0.66
TC15064 similar to UP|Q948P0 (Q948P0) Aspartic proteinase alpha,...    29  1.1
TC15364 weakly similar to UP|Q39048 (Q39048) CER2 gene, partial ...    29  1.4
TC15420                                                                27  4.2
BP041397                                                               27  5.5
BI420376                                                               27  7.2
TC9823 similar to UP|O22911 (O22911) At2g47370 protein, partial ...    26  9.4
AV415003                                                               26  9.4

>TC8957 weakly similar to UP|Q9ZUJ5 (Q9ZUJ5) T2K10.2 protein, partial (20%)
          Length = 724

 Score =  311 bits (796), Expect = 1e-85
 Identities = 152/215 (70%), Positives = 171/215 (78%), Gaps = 6/215 (2%)
 Frame = +1

Query: 1   MKKQQ-----LIFPLTISFSLTLLIVSARLFPDVPSWIPPGTLPVGPWDAFRNFTGCRQG 55
           MKK Q     +I  L   FS    +VSARLFPDVPS+IP G  P G WDAFRNF+GC +G
Sbjct: 79  MKKHQQPQLHIILTLVHLFSFGTAVVSARLFPDVPSFIPDGVPPSGAWDAFRNFSGCHRG 258

Query: 56  ENYNGLSNLKNYFQHFGYIPRSPKSNFSDDFDDDLQEAIKTYQKNFNLNVTGELDDMTLR 115
           ENY+GLSNLK+YF+ FGYIP +P SNFSDDFDD L+ AIKTYQKNFNLN+TG+LDD TLR
Sbjct: 259 ENYDGLSNLKSYFKRFGYIPHAPPSNFSDDFDDALEAAIKTYQKNFNLNITGDLDDKTLR 438

Query: 116 QVMLPRCGVADIINGTTTMN-AGKDTETTSNSDSKLRFHTVSHFTVFPGQPRWPEGKQEL 174
           Q+MLPRCGVADIINGTTTMN AG   ET S+ DS L FHTVSH+ +FPGQPR P GKQEL
Sbjct: 439 QIMLPRCGVADIINGTTTMNAAGNQDETASHGDSNLHFHTVSHYALFPGQPRGPAGKQEL 618

Query: 175 TYAFFPGNELTETVKSVFATAFARWSEVTTLKFTE 209
           TYAF+PGN L++ VKSVF  AFARWSEVTTL F E
Sbjct: 619 TYAFYPGNSLSDAVKSVFTGAFARWSEVTTLTFRE 723


>TC16638 weakly similar to UP|MEP1_SOYBN (P29136) Metalloendoproteinase 1
           precursor  (SMEP1) , partial (29%)
          Length = 621

 Score =  200 bits (508), Expect = 4e-52
 Identities = 105/138 (76%), Positives = 114/138 (82%), Gaps = 5/138 (3%)
 Frame = +2

Query: 241 AHAFSPRNGRFHLDAAEDWVVSGDVSKSSLPTAVDLESVAVHEIGHLLGLGHSSEEEAIM 300
           AHAFSP NGRFHLDAAEDWVVSGDVSKS+L TAVDLESVAVHEIGHLLGLGHSS EEAIM
Sbjct: 2   AHAFSPTNGRFHLDAAEDWVVSGDVSKSALATAVDLESVAVHEIGHLLGLGHSSVEEAIM 181

Query: 301 FPTISSRMKKVVLADDDVRGIQYLYGTNPSFNGSTVIS-SPERNIGNGG----CSLTSLW 355
           FPTISSR KKVVLA+DD++GIQYLYGTNPSFNGS+  + SPER+  +GG    C L S W
Sbjct: 182 FPTISSRTKKVVLAEDDIKGIQYLYGTNPSFNGSSATTFSPERDASDGGRRLSCFLASPW 361

Query: 356 SPWRLFSLLTFVLSHFLL 373
             W LFSLLT    H +L
Sbjct: 362 --WVLFSLLTSAFLHLVL 409


>TC14335 similar to UP|MEP1_SOYBN (P29136) Metalloendoproteinase 1 precursor
            (SMEP1) , partial (9%)
          Length = 1574

 Score =  166 bits (420), Expect = 6e-42
 Identities = 114/321 (35%), Positives = 156/321 (48%), Gaps = 3/321 (0%)
 Frame = +3

Query: 55  GENYNGLSNLKNYFQHFGYIPR---SPKSNFSDDFDDDLQEAIKTYQKNFNLNVTGELDD 111
           G++  GL N+K Y    GYI     +  SN +D F +DL+ AI  YQKNFNL VTG +D 
Sbjct: 249 GDSVKGLENIKKYLDALGYIKDLVLALNSNDTDKFTEDLKPAIMEYQKNFNLPVTGVIDQ 428

Query: 112 MTLRQVMLPRCGVADIINGTTTMNAGKDTETTSNSDSKLRFHTVSHFTVFPGQPRWPEGK 171
                ++ PRCGV D     TT+N G                     T    +P W   K
Sbjct: 429 NLYNTLIQPRCGVPDF----TTLNVG---------------------TTPAFKPWWKSEK 533

Query: 172 QELTYAFFPGNELTETVKSVFATAFARWSEVTTLKFTETTLYSGADIKIGFFNGDHGDGE 231
           +EL+YAF P N +++ +++VF  AF RWS  T+L FTET  +  +DIKI F   D G G 
Sbjct: 534 KELSYAFHPQNNVSDDIRTVFREAFDRWSNETSLNFTETATFDASDIKIAFVKWD-GKG- 707

Query: 232 PFDGSLGTLAHAFSPRNGRFHLDAAEDWVVSGDVSKSSLPTAVDLESVAVHEIGHLLGLG 291
              G +G     +S   G   LD  E +V               L +V +HEIGHLLGLG
Sbjct: 708 ---GRVGAAYTNYSMHAGGMFLDIDEQYV---------------LLNVVMHEIGHLLGLG 833

Query: 292 HSSEEEAIMFPTISSRMKKVVLADDDVRGIQYLYGTNPSFNGSTVISSPERNIGNGGCSL 351
           HS E+E+IM+P +    +K+   DDD + IQ + G N +  G++  SS      + G   
Sbjct: 834 HSYEKESIMYPIV--MREKIDFTDDDRKRIQKVTG-NSNNGGTSASSSGTTTDSSSGGGH 1004

Query: 352 TSLWSPWRLFSLLTFVLSHFL 372
            S+W    +      V   FL
Sbjct: 1005DSVWQRNLILGFAFMVFGIFL 1067


>BP078780 
          Length = 516

 Score = 88.2 bits (217), Expect = 2e-18
 Identities = 44/103 (42%), Positives = 68/103 (65%), Gaps = 3/103 (2%)
 Frame = +3

Query: 43  WDAFRN-FTG--CRQGENYNGLSNLKNYFQHFGYIPRSPKSNFSDDFDDDLQEAIKTYQK 99
           W+  ++ F G   R G+  +GLS +++Y   FGY+  +  SNF++++ ++ + AI+ +QK
Sbjct: 201 WNKLKSLFNGPKARIGDIVDGLSQVQDYLDLFGYLNSTLHSNFTNEYTENAKSAIEEFQK 380

Query: 100 NFNLNVTGELDDMTLRQVMLPRCGVADIINGTTTMNAGKDTET 142
           N+NL VTG+LD+     +  PRCGV DIINGTTTMN+ K   T
Sbjct: 381 NYNLQVTGQLDNNLYHVLSQPRCGVPDIINGTTTMNSRKANTT 509


>TC12158 homologue to UP|143A_SOYBN (Q96450) 14-3-3-like protein A (SGF14A),
           partial (54%)
          Length = 562

 Score = 30.4 bits (67), Expect = 0.50
 Identities = 27/98 (27%), Positives = 47/98 (47%), Gaps = 2/98 (2%)
 Frame = -2

Query: 264 DVSKSSLPTAVDLESVAVHEIGHLLGLGHSSEEEAIMFPTISS--RMKKVVLADDDVRGI 321
           D  +  +P+ + L S ++  +  L+    SS      FP +SS   M++++L +  +  I
Sbjct: 468 DSRRFKIPSQILLSSASIFPLYSLITETWSS------FPLLSSFCSMEEMILQEALLAPI 307

Query: 322 QYLYGTNPSFNGSTVISSPERNIGNGGCSLTSLWSPWR 359
            +LY T   F  STV SS   ++     S+ S  S +R
Sbjct: 306 TFLYATESKFLSSTVNSSASTDLAT--FSMNSTISSYR 199


>AV417355 
          Length = 380

 Score = 27.3 bits (59), Expect(2) = 0.66
 Identities = 16/44 (36%), Positives = 22/44 (49%)
 Frame = +2

Query: 131 TTTMNAGKDTETTSNSDSKLRFHTVSHFTVFPGQPRWPEGKQEL 174
           TTT N+   T TT+ + S     ++S F+V  G P    G Q L
Sbjct: 137 TTTTNSSYTTTTTTTTSSGSGSSSISKFSVRSGDPAAGPGGQIL 268



 Score = 21.2 bits (43), Expect(2) = 0.66
 Identities = 7/13 (53%), Positives = 11/13 (83%)
 Frame = +1

Query: 197 ARWSEVTTLKFTE 209
           +RWS++T LKF +
Sbjct: 250 SRWSDLTYLKFED 288


>TC15064 similar to UP|Q948P0 (Q948P0) Aspartic proteinase alpha, partial
           (77%)
          Length = 1444

 Score = 29.3 bits (64), Expect = 1.1
 Identities = 18/52 (34%), Positives = 26/52 (49%)
 Frame = -1

Query: 79  KSNFSDDFDDDLQEAIKTYQKNFNLNVTGELDDMTLRQVMLPRCGVADIING 130
           K++FS  FD+ L   +     NFN+ +T E  D +     L RC + DI  G
Sbjct: 205 KASFSGSFDEILM--LDNSTTNFNMILTEETRDCSRSIGYLARCSIFDICFG 56


>TC15364 weakly similar to UP|Q39048 (Q39048) CER2 gene, partial (7%)
          Length = 835

 Score = 28.9 bits (63), Expect = 1.4
 Identities = 17/47 (36%), Positives = 21/47 (44%), Gaps = 5/47 (10%)
 Frame = -2

Query: 2   KKQQLIFPLTISFSLTLLIVSA-----RLFPDVPSWIPPGTLPVGPW 43
           K    I P T+S  L+  I S+       FP +P WI     P GPW
Sbjct: 360 KSPSSIIPFTLSSDLSFTISSSGNIMITNFPSIPFWIE----PFGPW 232


>TC15420 
          Length = 1023

 Score = 27.3 bits (59), Expect = 4.2
 Identities = 15/38 (39%), Positives = 20/38 (52%), Gaps = 4/38 (10%)
 Frame = +2

Query: 132 TTMNAGKDT----ETTSNSDSKLRFHTVSHFTVFPGQP 165
           T++N  + T     T S   + L  H+ SHFTVFP  P
Sbjct: 26  TSLNIVRTTPLCVSTKSIFSNLLLSHSTSHFTVFPSNP 139


>BP041397 
          Length = 397

 Score = 26.9 bits (58), Expect = 5.5
 Identities = 15/32 (46%), Positives = 20/32 (61%), Gaps = 1/32 (3%)
 Frame = -2

Query: 343 NIGNG-GCSLTSLWSPWRLFSLLTFVLSHFLL 373
           NIG+G   S   LW+  R+F  L F+ SH+LL
Sbjct: 228 NIGHGLPSSWLFLWAGMRIFLSLIFL*SHYLL 133


>BI420376 
          Length = 562

 Score = 26.6 bits (57), Expect = 7.2
 Identities = 11/21 (52%), Positives = 12/21 (56%)
 Frame = +1

Query: 165 PRWPEGKQELTYAFFPGNELT 185
           PRWPE +    YA  P  ELT
Sbjct: 487 PRWPESRDWAWYANVPHKELT 549


>TC9823 similar to UP|O22911 (O22911) At2g47370 protein, partial (23%)
          Length = 741

 Score = 26.2 bits (56), Expect = 9.4
 Identities = 29/116 (25%), Positives = 53/116 (45%), Gaps = 5/116 (4%)
 Frame = +3

Query: 190 SVFATAFARWSEVTTLKFTETTLYSGADIKIGFFNGDHGDGEPFDGSLGTLAHAFSPRNG 249
           ++F    A+  EV  +K     LY G  +++               S   L  A SP +G
Sbjct: 270 NLFKIDLAKGEEVKIIKVEGGPLYFGDGLELL--------------SPNKLVVAGSP-SG 404

Query: 250 RFHLDAAEDWVVSGDVS-----KSSLPTAVDLESVAVHEIGHLLGLGHSSEEEAIM 300
           R  +++A+ W  +  V+     K  L TA  ++   V+ + H+LG+G+  ++ AI+
Sbjct: 405 RL-VESADGWDTASVVATFSGPKHRLATAATVKDGKVY-LNHMLGMGYPKKKHAIV 566


>AV415003 
          Length = 265

 Score = 26.2 bits (56), Expect = 9.4
 Identities = 11/16 (68%), Positives = 11/16 (68%)
 Frame = -2

Query: 221 GFFNGDHGDGEPFDGS 236
           G FNG  G GE FDGS
Sbjct: 168 GCFNGGGGGGESFDGS 121


  Database: LJGI
    Posted date:  Jul 30, 2004 11:16 AM
  Number of letters in database: 14,692,800
  Number of sequences in database:  28,460
  
Lambda     K      H
   0.319    0.137    0.418 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,611,954
Number of Sequences: 28460
Number of extensions: 89691
Number of successful extensions: 451
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 443
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 445
length of query: 373
length of database: 4,897,600
effective HSP length: 92
effective length of query: 281
effective length of database: 2,279,280
effective search space: 640477680
effective search space used: 640477680
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 56 (26.2 bits)