BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146587.12 - phase: 0 /pseudo
(215 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC14231 123 1e-36
TC10837 105 4e-28
AV767701 111 1e-25
AV427493 107 2e-24
TC10139 similar to UP|Q9SVU5 (Q9SVU5) Proline-rich APG-like prot... 89 1e-22
TC14230 96 6e-21
TC12378 90 3e-19
TC11616 similar to UP|Q9SVU5 (Q9SVU5) Proline-rich APG-like prot... 72 7e-14
BP040561 70 3e-13
AV419166 55 1e-08
AU089279 53 5e-08
BP080481 52 1e-07
BP041040 51 2e-07
TC11748 weakly similar to UP|O82076 (O82076) Lipase homolog (Fra... 50 2e-07
AW720418 47 3e-06
BG662032 44 2e-05
TC12983 44 2e-05
TC8381 43 4e-05
TC16911 43 5e-05
CB827176 40 2e-04
>TC14231
Length = 803
Score = 123 bits (309), Expect(2) = 1e-36
Identities = 54/89 (60%), Positives = 72/89 (80%)
Frame = +2
Query: 72 RKLLERLYDIGARRVLVTGTGPLGCVPAEMAMRGTDGGCSAELQRAATLYNPQLQHMVQG 131
R++L ++YD+GARRVLVTGTGP+GCVP +AMR G C EL RAA+LYNPQL +M++G
Sbjct: 5 REVLRKMYDLGARRVLVTGTGPMGCVPPXLAMRSRTGDCDVELXRAASLYNPQLVNMIKG 184
Query: 132 LNKKIGKDVFIATNTALIHSDFVTNPKAY 160
LN+++G DVFIA N +H DF++NP+AY
Sbjct: 185 LNQELGADVFIAANAYQMHMDFISNPRAY 271
Score = 45.1 bits (105), Expect(2) = 1e-36
Identities = 21/44 (47%), Positives = 26/44 (58%)
Frame = +3
Query: 167 KEPTTGLVCAHHSLIYAQIETCMHFGMHSIHLKRQTKLLWSEFC 210
K T GL CAH AQ ET HFG+HSIH + Q + L++ C
Sbjct: 306 KAHTMGLGCAHRPQTCAQTETFTHFGIHSIHQREQVESLFNRSC 437
>TC10837
Length = 667
Score = 105 bits (261), Expect(2) = 4e-28
Identities = 47/73 (64%), Positives = 58/73 (79%)
Frame = +2
Query: 88 VTGTGPLGCVPAEMAMRGTDGGCSAELQRAATLYNPQLQHMVQGLNKKIGKDVFIATNTA 147
VTGTG +GCVPAE+A+R +G C ELQRAA LYNPQL M++GLNK+IG DVF+A N
Sbjct: 2 VTGTGSMGCVPAELALRSRNGECDVELQRAAALYNPQLVEMIKGLNKEIGADVFVAANAY 181
Query: 148 LIHSDFVTNPKAY 160
+H DFV+NP+AY
Sbjct: 182 QMHMDFVSNPQAY 220
Score = 35.0 bits (79), Expect(2) = 4e-28
Identities = 19/38 (50%), Positives = 20/38 (52%)
Frame = +3
Query: 163 LVVDKEPTTGLVCAHHSLIYAQIETCMHFGMHSIHLKR 200
L V K T GL A H IET HFGMHS H K+
Sbjct: 243 LAVGKARTMGLDSALHFPTCVTIETYTHFGMHSTHQKK 356
>AV767701
Length = 622
Score = 111 bits (277), Expect = 1e-25
Identities = 52/63 (82%), Positives = 57/63 (89%)
Frame = +3
Query: 1 MYRQLDYFEEYQHRVASMIGAARAEKLVNKALVLITVGGNDFVNNYYLVPYSARSREYSL 60
MYRQLDYFEEYQHR+AS IG + + LV+KALVLITVGGNDFVNNYYLVPYSARSR+YSL
Sbjct: 432 MYRQLDYFEEYQHRLASQIGVTKTKALVDKALVLITVGGNDFVNNYYLVPYSARSRQYSL 611
Query: 61 QDY 63
DY
Sbjct: 612 PDY 620
>AV427493
Length = 217
Score = 107 bits (267), Expect = 2e-24
Identities = 49/71 (69%), Positives = 62/71 (87%)
Frame = +2
Query: 47 YLVPYSARSREYSLQDYVKFLIIEYRKLLERLYDIGARRVLVTGTGPLGCVPAEMAMRGT 106
+LVP+SARSR+++L DYV +LI EYRK+L RLY++GARRV+VTGTGPLGCVPAE+A R
Sbjct: 5 FLVPFSARSRQFALPDYVVYLISEYRKILARLYELGARRVMVTGTGPLGCVPAELAQRSR 184
Query: 107 DGGCSAELQRA 117
+G CS ELQ+A
Sbjct: 185 NGECSPELQQA 217
>TC10139 similar to UP|Q9SVU5 (Q9SVU5) Proline-rich APG-like protein,
partial (40%)
Length = 595
Score = 88.6 bits (218), Expect(2) = 1e-22
Identities = 39/71 (54%), Positives = 51/71 (70%)
Frame = +2
Query: 90 GTGPLGCVPAEMAMRGTDGGCSAELQRAATLYNPQLQHMVQGLNKKIGKDVFIATNTALI 149
GTGPLGCVPAE+A+ +G C+ ELQRA L+NPQL ++ LN +IG VFI+ N +
Sbjct: 2 GTGPLGCVPAELALHSRNGECAVELQRAVNLFNPQLVQLINQLNTEIGSHVFISANAFAM 181
Query: 150 HSDFVTNPKAY 160
H DFV +P+AY
Sbjct: 182 HLDFVNDPQAY 214
Score = 33.5 bits (75), Expect(2) = 1e-22
Identities = 24/53 (45%), Positives = 27/53 (50%), Gaps = 5/53 (9%)
Frame = +3
Query: 157 PKAYELL-----VVDKEPTTGLVCAHHSLIYAQIETCMHFGMHSIHLKRQTKL 204
PK +LL VV KE T L A LI AQ +HFG SIHL+ Q L
Sbjct: 204 PKHMDLLHQK*HVVVKERTMELDSALQPLICAQTGIYLHFGTPSIHLREQIDL 362
>TC14230
Length = 740
Score = 95.5 bits (236), Expect = 6e-21
Identities = 53/99 (53%), Positives = 67/99 (67%), Gaps = 2/99 (2%)
Frame = +1
Query: 3 RQLDYFEEYQHRVASMIGAARAEKLVNKALVLITVGGNDFVNNYYLVPYSARSREYSLQD 62
+QL F++YQ RV++ IG LVN+AL LIT+GGNDFVNNYYLVPYSARSR+YSL D
Sbjct: 442 KQLKLFQQYQKRVSAHIGREGTRNLVNRALFLITLGGNDFVNNYYLVPYSARSRQYSLPD 621
Query: 63 YVKFLIIEYRKLLERLYDI-GARRVLVTGT-GPLGCVPA 99
YV+++I E + E I R+ G G GCVP+
Sbjct: 622 YVRYIISELPQSSEEDVMIWELRKGSGNGVQGQWGCVPS 738
>TC12378
Length = 464
Score = 89.7 bits (221), Expect = 3e-19
Identities = 40/54 (74%), Positives = 44/54 (81%)
Frame = +2
Query: 161 ELLVVDKEPTTGLVCAHHSLIYAQIETCMHFGMHSIHLKRQTKLLWSEFCQVLK 214
+LLVV K+PT GL CAHHSL YA E CMHFG+HSIHLKRQT LLW + CQVLK
Sbjct: 134 KLLVVGKDPTMGLGCAHHSLTYAHTEICMHFGIHSIHLKRQTNLLWRQLCQVLK 295
Score = 65.5 bits (158), Expect = 7e-12
Identities = 32/72 (44%), Positives = 44/72 (60%), Gaps = 13/72 (18%)
Frame = +1
Query: 122 NPQLQHMVQGLNKKIGKDVFIATNTALIHSDFVTNPKAY-----ELLVVDKEPTTGL--- 173
NPQL HM+Q LNKK G++VF+A NT +H+DF+TNPKAY E+ + P G+
Sbjct: 1 NPQLLHMIQALNKKFGREVFVAANTGQMHNDFITNPKAYGFTTSEIACCGQGPYNGIGLC 180
Query: 174 -----VCAHHSL 180
+C H +L
Sbjct: 181 TPLSNLCPHRNL 216
>TC11616 similar to UP|Q9SVU5 (Q9SVU5) Proline-rich APG-like protein,
partial (41%)
Length = 569
Score = 72.0 bits (175), Expect = 7e-14
Identities = 31/47 (65%), Positives = 41/47 (86%)
Frame = +2
Query: 1 MYRQLDYFEEYQHRVASMIGAARAEKLVNKALVLITVGGNDFVNNYY 47
++RQL+YF+EYQ RV+++IG ++LVN ALVLIT+GGNDFVNNYY
Sbjct: 428 IFRQLEYFDEYQQRVSALIGPEETQRLVNGALVLITLGGNDFVNNYY 568
>BP040561
Length = 515
Score = 70.1 bits (170), Expect = 3e-13
Identities = 32/48 (66%), Positives = 39/48 (80%)
Frame = +1
Query: 1 MYRQLDYFEEYQHRVASMIGAARAEKLVNKALVLITVGGNDFVNNYYL 48
MY+QL F+ YQ R+++ IG +KLVNKALVLIT+GGNDFVNNYYL
Sbjct: 370 MYKQLKLFQHYQQRLSAQIGPEGTKKLVNKALVLITLGGNDFVNNYYL 513
>AV419166
Length = 270
Score = 54.7 bits (130), Expect = 1e-08
Identities = 29/93 (31%), Positives = 46/93 (49%)
Frame = +3
Query: 30 KALVLITVGGNDFVNNYYLVPYSARSREYSLQDYVKFLIIEYRKLLERLYDIGARRVLVT 89
+ L + +G ND+ NY+L SR Y+ + Y K L + L+ L+ +GAR+ +V
Sbjct: 3 QCLYYVYIGTNDYEQNYFLPDLFNTSRTYTPEQYAKVLTHQLSHYLKALHHVGARKTVVV 182
Query: 90 GTGPLGCVPAEMAMRGTDGGCSAELQRAATLYN 122
LGC+P +G C + AA L+N
Sbjct: 183 SLDRLGCIPKVF----VNGSCIEKQNAAAFLFN 269
>AU089279
Length = 169
Score = 52.8 bits (125), Expect = 5e-08
Identities = 25/46 (54%), Positives = 33/46 (71%)
Frame = +3
Query: 113 ELQRAATLYNPQLQHMVQGLNKKIGKDVFIATNTALIHSDFVTNPK 158
+LQRAA+LYNPQL +M+ GL G DVFIA N + DF++NP+
Sbjct: 6 QLQRAASLYNPQLVNMIXGL*VDTGPDVFIAANAYQMLMDFISNPR 143
>BP080481
Length = 489
Score = 51.6 bits (122), Expect = 1e-07
Identities = 24/68 (35%), Positives = 42/68 (61%)
Frame = -3
Query: 1 MYRQLDYFEEYQHRVASMIGAARAEKLVNKALVLITVGGNDFVNNYYLVPYSARSREYSL 60
+Y+Q++ F+ Q + S IG AEK +A ++ +G NDF+NN YL+P + S Y+
Sbjct: 316 LYKQIELFQGTQKLITSKIGKEEAEKFFQEARYVVALGSNDFINN-YLMPVYSDSWTYND 140
Query: 61 QDYVKFLI 68
Q ++ +L+
Sbjct: 139 QTFMDYLV 116
>BP041040
Length = 550
Score = 50.8 bits (120), Expect = 2e-07
Identities = 16/50 (32%), Positives = 38/50 (76%)
Frame = +3
Query: 1 MYRQLDYFEEYQHRVASMIGAARAEKLVNKALVLITVGGNDFVNNYYLVP 50
++++++Y+++Y+ ++ + +G +A ++V +AL L+++G NDF+ NYY P
Sbjct: 384 LWKEVEYYKDYRQKLVAYLGDEKANEIVKEALYLVSIGTNDFLENYYTFP 533
>TC11748 weakly similar to UP|O82076 (O82076) Lipase homolog (Fragment),
partial (48%)
Length = 679
Score = 50.4 bits (119), Expect = 2e-07
Identities = 29/95 (30%), Positives = 43/95 (44%), Gaps = 4/95 (4%)
Frame = +3
Query: 70 EYRKLLERLYDIGARRVLVTGTGPLGCVPAEMAMRGTDG----GCSAELQRAATLYNPQL 125
E ++ LY+ GAR+ V TGPLGC+P +A+ GC + AA L+N L
Sbjct: 6 EIENAVKSLYNEGARKFWVHNTGPLGCLPKILALAQKKDLDLFGCLSSYNSAARLFNEAL 185
Query: 126 QHMVQGLNKKIGKDVFIATNTALIHSDFVTNPKAY 160
H Q L ++ + + I D + N Y
Sbjct: 186 YHSSQKLRTRLKDATLVYVDIYAIKHDLIANATKY 290
>AW720418
Length = 500
Score = 46.6 bits (109), Expect = 3e-06
Identities = 34/137 (24%), Positives = 59/137 (42%), Gaps = 7/137 (5%)
Frame = +3
Query: 29 NKALVLITVGGNDFVNNYYLVPYSARSREYSLQDYVKFLIIEYRKLLERLYDIGARRVLV 88
++AL + +G ND + S E L+ + ++ ++ + +E+LY GAR +
Sbjct: 81 SRALYMFDIGQNDLSYGFQY------SSEEQLRASLPNILSQFSQAVEQLYXEGARVFWI 242
Query: 89 TGTGPLGCVPAEMAMRGTD-------GGCSAELQRAATLYNPQLQHMVQGLNKKIGKDVF 141
TGP+GC+P G GC A +N QL++ V L KK+
Sbjct: 243 HNTGPIGCLPLNFLAYGKSKKGNVDANGCVISQNGIAHEFNLQLKNQVLQLRKKLPLAKL 422
Query: 142 IATNTALIHSDFVTNPK 158
I + + V+N +
Sbjct: 423 IYVDVYKAKYELVSNAR 473
>BG662032
Length = 421
Score = 44.3 bits (103), Expect = 2e-05
Identities = 23/91 (25%), Positives = 43/91 (46%), Gaps = 1/91 (1%)
Frame = +1
Query: 71 YRKLLERLYDIGARRVLVTGTGPLGCVPAEMAMRG-TDGGCSAELQRAATLYNPQLQHMV 129
+R L RLY + AR+ ++ GP+GC+P + + + C + A YN +L+ ++
Sbjct: 19 FRAQLTRLYRLDARKFVIGNVGPIGCIPYQKTINQLNENECVDLPNKLALQYNARLKDLM 198
Query: 130 QGLNKKIGKDVFIATNTALIHSDFVTNPKAY 160
LN + F+ N + + + N Y
Sbjct: 199 AELNDNLPGATFVVANVYDLVMELIKNYDKY 291
>TC12983
Length = 515
Score = 43.9 bits (102), Expect = 2e-05
Identities = 20/33 (60%), Positives = 26/33 (78%)
Frame = +3
Query: 4 QLDYFEEYQHRVASMIGAARAEKLVNKALVLIT 36
QL YF +YQ RV+++IG A+ LVN+ALVLIT
Sbjct: 417 QLGYFRQYQQRVSALIGEKEAQNLVNQALVLIT 515
>TC8381
Length = 844
Score = 43.1 bits (100), Expect = 4e-05
Identities = 28/72 (38%), Positives = 40/72 (54%), Gaps = 3/72 (4%)
Frame = +3
Query: 82 GARRVLVTGTGPLGCVPAEMAMRGTDGGCSAELQRAATLYNPQLQHMVQGLNKKI---GK 138
GAR+ ++ G LGC+P A +G C + AA L+N QL+ +V N KI K
Sbjct: 9 GARKTIMVGMDRLGCIPK--ARLTNNGSCIEKENVAAFLFNDQLKALVDRYNHKILPDSK 182
Query: 139 DVFIATNTALIH 150
+FI +TA+IH
Sbjct: 183 FIFI-NSTAIIH 215
>TC16911
Length = 629
Score = 42.7 bits (99), Expect = 5e-05
Identities = 29/102 (28%), Positives = 51/102 (49%)
Frame = +3
Query: 60 LQDYVKFLIIEYRKLLERLYDIGARRVLVTGTGPLGCVPAEMAMRGTDGGCSAELQRAAT 119
++ + + LI + L+R+ +G +V V P+GC+P + + C L +
Sbjct: 3 IKSFTESLIKQLTLNLKRVQSLGIGKVAVGLLQPVGCLPG-LTVVSFHLSCIDPLNLVSK 179
Query: 120 LYNPQLQHMVQGLNKKIGKDVFIATNTALIHSDFVTNPKAYE 161
+N L VQ LNK++GK VFI T +++ F+T K +
Sbjct: 180 THNQMLLQTVQELNKEMGKPVFI---TLDLYNSFLTTIKTMQ 296
>CB827176
Length = 503
Score = 40.4 bits (93), Expect = 2e-04
Identities = 26/99 (26%), Positives = 46/99 (46%)
Frame = +1
Query: 1 MYRQLDYFEEYQHRVASMIGAARAEKLVNKALVLITVGGNDFVNNYYLVPYSARSREYSL 60
M Q+D+FE+ A L N +L L++ GND+ + Y +
Sbjct: 145 MTTQIDFFEQLIEE-----NVYNASDL-NNSLALLSALGNDYFD------YLKHGSIWGF 288
Query: 61 QDYVKFLIIEYRKLLERLYDIGARRVLVTGTGPLGCVPA 99
+ +V L+ + L R+ +G ++++VT P GC+PA
Sbjct: 289 KSFVDSLVNQMTTNLIRIQKLGVKKIVVTSLPPFGCIPA 405
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.324 0.138 0.410
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,515,983
Number of Sequences: 28460
Number of extensions: 43800
Number of successful extensions: 235
Number of sequences better than 10.0: 58
Number of HSP's better than 10.0 without gapping: 231
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 232
length of query: 215
length of database: 4,897,600
effective HSP length: 86
effective length of query: 129
effective length of database: 2,450,040
effective search space: 316055160
effective search space used: 316055160
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 53 (25.0 bits)
Medicago: description of AC146587.12