BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146585.12 + phase: 0 /pseudo
(942 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC16249 similar to UP|O63067 (O63067) Aspartokinase-homoserine d... 207 5e-54
AV418814 181 5e-46
TC9572 homologue to UP|O63067 (O63067) Aspartokinase-homoserine ... 174 8e-44
TC17748 similar to UP|O63067 (O63067) Aspartokinase-homoserine d... 119 3e-27
AV409108 118 5e-27
BP056396 93 8e-25
TC14781 homologue to UP|Q9XHC5 (Q9XHC5) Precursor monofunctional... 94 1e-19
TC10849 similar to UP|Q9XHC5 (Q9XHC5) Precursor monofunctional a... 83 2e-16
AV779391 77 1e-14
BP054054 59 4e-10
TC17093 weakly similar to UP|PYRH_LACPL (Q88VJ6) Uridylate kinas... 39 0.003
TC10932 homologue to UP|Q39889 (Q39889) Heat shock protein , par... 35 0.072
BP055392 30 1.4
TC12043 weakly similar to UP|Q9SND9 (Q9SND9) Anthranilate N-hydr... 28 5.2
TC15287 similar to UP|Q9FMY5 (Q9FMY5) U2 snRNP auxiliary factor,... 28 5.2
TC8944 similar to UP|Q9SIR2 (Q9SIR2) At2g25310 protein, partial ... 28 5.2
TC14147 similar to UP|ROC1_NICSY (Q08935) 29 kDa ribonucleoprote... 28 8.8
>TC16249 similar to UP|O63067 (O63067) Aspartokinase-homoserine
dehydrogenase (HDH) (Aspartate kinase) , partial (13%)
Length = 590
Score = 207 bits (528), Expect = 5e-54
Identities = 101/114 (88%), Positives = 110/114 (95%)
Frame = +3
Query: 829 PLRACASAQEFMQQLPKFDQEFAKKQEDADNAGEVLRYVGVVDVTNKKGVVELRKYKKDH 888
PLR CASAQ+F++QLPKFD+E KKQEDA++AGEVLRYVGVVDV N+KGVVELR+YKKDH
Sbjct: 3 PLRVCASAQDFIRQLPKFDKELTKKQEDAESAGEVLRYVGVVDVRNEKGVVELRRYKKDH 182
Query: 889 PFAQLSGSDNIIAFTTRRYKNQPLIVRGPGAGAQVTAGGIFSDILRLASYLGAP 942
PFAQLSGSDNIIAFTTRRY+NQPLIVRGPGAGAQVTAGGIFSDILRLASYLGAP
Sbjct: 183 PFAQLSGSDNIIAFTTRRYQNQPLIVRGPGAGAQVTAGGIFSDILRLASYLGAP 344
>AV418814
Length = 420
Score = 181 bits (459), Expect = 5e-46
Identities = 106/166 (63%), Positives = 118/166 (70%)
Frame = +3
Query: 497 KMASTPGVSATLFNALAKANINVRAIAQGCSEYNITVVIKREDSIKALRAVHSRFYLSRT 556
KMASTPGVSATLFNALAKANINVRAIAQGCSEYNITVV+KRED +KALRAVHSRFYLSRT
Sbjct: 3 KMASTPGVSATLFNALAKANINVRAIAQGCSEYNITVVLKREDCVKALRAVHSRFYLSRT 182
Query: 557 TIAMGIIGPGLIGSTLLDQLRDQAKHSHLYISYLLHTRRLLEICGKLMQQFIQCRLQF*K 616
TIAMGIIGPGLIGSTLLDQLRDQA + L R++ I G
Sbjct: 183 TIAMGIIGPGLIGSTLLDQLRDQASILKEEFNIDL---RVMGILG--------------S 311
Query: 617 KNLTLICV*WA*LAQSQCFSVMSKWKELREERGEVANLEKFAQHVH 662
K++ L S +++W+ELR+E GEVANLEKF QHVH
Sbjct: 312 KSMLL----------SDWGIDLARWRELRDESGEVANLEKFVQHVH 419
>TC9572 homologue to UP|O63067 (O63067) Aspartokinase-homoserine
dehydrogenase (HDH) (Aspartate kinase) , partial (11%)
Length = 510
Score = 174 bits (440), Expect = 8e-44
Identities = 87/97 (89%), Positives = 92/97 (94%)
Frame = +1
Query: 846 FDQEFAKKQEDADNAGEVLRYVGVVDVTNKKGVVELRKYKKDHPFAQLSGSDNIIAFTTR 905
FDQ FAK E+A+ AGEVLRYVGVVDVT+KKGVVELR+YKKDHPFA LSGSDNIIAFTTR
Sbjct: 1 FDQMFAKTLEEAEYAGEVLRYVGVVDVTSKKGVVELRRYKKDHPFAPLSGSDNIIAFTTR 180
Query: 906 RYKNQPLIVRGPGAGAQVTAGGIFSDILRLASYLGAP 942
RYK+QPLIVRGPGAGAQVTAGGIFSDILRLASYLGAP
Sbjct: 181 RYKDQPLIVRGPGAGAQVTAGGIFSDILRLASYLGAP 291
>TC17748 similar to UP|O63067 (O63067) Aspartokinase-homoserine
dehydrogenase (HDH) (Aspartate kinase) , partial (8%)
Length = 658
Score = 119 bits (297), Expect = 3e-27
Identities = 59/84 (70%), Positives = 70/84 (83%)
Frame = +2
Query: 331 LKTLSYQEAWEMSYFGANVLHPRTIIPVMRYGIPILIRNIFNLSAPGTKICHPVVSDYED 390
L+ L+++ SYFGANVLHP TII VM+YGIPILIRNIFNLSA GTKI HP V+D ED
Sbjct: 56 LRRLTFKLPAVQSYFGANVLHPHTIIHVMQYGIPILIRNIFNLSASGTKIRHPSVNDSED 235
Query: 391 KSNLQNYVKGFATIDNLALVNVEG 414
NL+++V+GFATIDNLAL+NVEG
Sbjct: 236 MMNLKSFVEGFATIDNLALINVEG 307
>AV409108
Length = 359
Score = 118 bits (295), Expect = 5e-27
Identities = 68/109 (62%), Positives = 81/109 (73%), Gaps = 1/109 (0%)
Frame = +3
Query: 1 MASSLSSSLSHFSRISVTSLQHDYNNKIPADSQCRHFLLSRRFHSLRKGITL-PRRRESP 59
MAS +SSL HFS IS ++ D KI + QC FL S R HSLRK ++L PR +SP
Sbjct: 39 MASLSASSLYHFSTISPSNNTPDIT-KI-SQCQCLPFLPSHRSHSLRKALSLLPRGNQSP 212
Query: 60 SSGICASLTDVSVNVAVEEKELSKGDSWSVHKFGGTCMGSSQRIKNVGD 108
S+ I ASLTDVS +V VEE++L KG++WSVHKFGGTCMGSSQRIKNVGD
Sbjct: 213 STKISASLTDVSPSVLVEEQQLQKGETWSVHKFGGTCMGSSQRIKNVGD 359
>BP056396
Length = 368
Score = 92.8 bits (229), Expect(2) = 8e-25
Identities = 44/55 (80%), Positives = 49/55 (89%)
Frame = -2
Query: 871 DVTNKKGVVELRKYKKDHPFAQLSGSDNIIAFTTRRYKNQPLIVRGPGAGAQVTA 925
DV N+KGVVELR+Y+KDHPFA LSGSD IIAF TRRY+N PLIVRGPGAGA VT+
Sbjct: 367 DVRNEKGVVELRRYQKDHPFAPLSGSDKIIAFPTRRYQNPPLIVRGPGAGAPVTS 203
Score = 38.9 bits (89), Expect(2) = 8e-25
Identities = 18/21 (85%), Positives = 19/21 (89%)
Frame = -3
Query: 922 QVTAGGIFSDILRLASYLGAP 942
Q AGGIFSD+LRLASYLGAP
Sbjct: 213 QSPAGGIFSDLLRLASYLGAP 151
>TC14781 homologue to UP|Q9XHC5 (Q9XHC5) Precursor monofunctional
aspartokinase (Aspartate kinase) , partial (40%)
Length = 1084
Score = 94.0 bits (232), Expect = 1e-19
Identities = 61/222 (27%), Positives = 120/222 (53%), Gaps = 1/222 (0%)
Frame = +3
Query: 334 LSYQEAWEMSYFGANVLHPRTIIPVMRYGIPILIRNIFNLSAPGTKICHPVVSDYEDKSN 393
L++ EA E++YFGA VLHP+++ P IP+ ++N +N APGT ++S D S
Sbjct: 36 LTFDEAAELAYFGAQVLHPQSMRPAGERDIPVRVKNFYNPKAPGT-----LISKSRDMS- 197
Query: 394 LQNYVKGFATIDNLALVNVEGTGMAGVPGTASAIFAAVKDVGANVIMISQASSEHSVCFA 453
+ + N+ ++++ T M G G + +F+ +D+G +V ++ A+SE SV
Sbjct: 198 -KAVLTSIVLKRNVTMLDIVSTRMLGQYGFLAKVFSIFEDLGISVDVV--ATSEVSVSLT 368
Query: 454 VPEKEVKAVAEALQSRFRQALDN-GRLSQVAVIPNCSILAAVGQKMASTPGVSATLFNAL 512
+ ++ + +Q ++ +++ V ++ N SI++ +G S+ + F L
Sbjct: 369 LDPSKL-WTRDLIQQELDHVVEELEKIAVVNLLQNXSIISLIGNVQRSSL-ILEKAFRVL 542
Query: 513 AKANINVRAIAQGCSEYNITVVIKREDSIKALRAVHSRFYLS 554
+ V+ I+QG S+ NI++V+ ++ + +RA+HS F+ S
Sbjct: 543 RSLGVTVQMISQGASKVNISLVVNDNEAEQCVRALHSAFFES 668
>TC10849 similar to UP|Q9XHC5 (Q9XHC5) Precursor monofunctional
aspartokinase (Aspartate kinase) , partial (43%)
Length = 902
Score = 82.8 bits (203), Expect = 2e-16
Identities = 60/219 (27%), Positives = 111/219 (50%), Gaps = 7/219 (3%)
Frame = +1
Query: 89 VHKFGGTCMGSSQRIKNVGDIVLNDDSERKLVVVSAMSKVTDMMYDLINKAQS---RDES 145
V KFGG+ + S++R+K + +VL+ ER +VV+SAM K T+ + KA S + S
Sbjct: 244 VMKFGGSSVASAERMKEIASLVLSFPEERPIVVLSAMGKTTNKLLLAGEKAVSCGVSNAS 423
Query: 146 YISSLDAVLEKHSATAHDILDGETLAIFLSKLHEDISNLKAMLRAIYIAGHVTESFTDFV 205
I L + + H T + L + S + +++ L+ +L+ I + +T+ D++
Sbjct: 424 TIDELSFIKDLHLRTV------DQLGLDRSVIAKNLEELEQLLKGIAMMRELTKRTQDYL 585
Query: 206 VGHGELWSAQMLSLVIRKNGIDCKWMDTREVLIVNP---TSSNQVDPDYLESERRLEKWY 262
V GE S ++ + + K G+ + D E+ + T+++ ++ Y +RL +
Sbjct: 586 VSFGECMSTRIFAAYLNKTGVKARQYDAFEIGFITTDDFTNADILEATYPAVAKRLHGDW 765
Query: 263 SLNPCKVIIATGFIASTPENIP-TTLKRDGSDFSAAIMG 300
+P + I TGF+ ++ TTL R GSD +A +G
Sbjct: 766 LSDPA-IPIVTGFLGKARKSCAVTTLGRGGSDLTATTIG 879
>AV779391
Length = 498
Score = 77.0 bits (188), Expect = 1e-14
Identities = 48/159 (30%), Positives = 83/159 (52%), Gaps = 4/159 (2%)
Frame = +2
Query: 197 VTESFTDFVVGHGELWSAQMLSLVIRKNGIDCKWMDTREVLIVNP---TSSNQVDPDYLE 253
+T D++V GE S ++ + + K G+ + D E+ + T+++ ++ Y
Sbjct: 23 LTPRTQDYLVSFGECMSTRIFAAYLNKLGVKARQYDAFEIGFITTDDFTNADILEATYPA 202
Query: 254 SERRLEKWYSLNPCKVIIATGFIASTPENIP-TTLKRDGSDFSAAIMGSLFRARQVTIWT 312
+RL + +P II TGF+ ++ T+L R GSD +A +G ++ +W
Sbjct: 203 VAKRLHSDWVSDPAIPII-TGFLGKARKSCAVTSLGRGGSDLTATTIGKALG*PEIQVWK 379
Query: 313 DVDGVYSADPRKVSEAVILKTLSYQEAWEMSYFGANVLH 351
DVDGV + DP +A + L++ EA E++YFGA VLH
Sbjct: 380 DVDGVLTCDPYICPQAEPVPYLTFDEAAELAYFGAQVLH 496
>BP054054
Length = 360
Score = 58.5 bits (140), Expect(2) = 4e-10
Identities = 28/32 (87%), Positives = 29/32 (90%)
Frame = -3
Query: 911 PLIVRGPGAGAQVTAGGIFSDILRLASYLGAP 942
PLIVRGPGA A VT GGIFSD+LRLASYLGAP
Sbjct: 295 PLIVRGPGARAPVTTGGIFSDLLRLASYLGAP 200
Score = 23.5 bits (49), Expect(2) = 4e-10
Identities = 9/13 (69%), Positives = 12/13 (92%)
Frame = -2
Query: 897 DNIIAFTTRRYKN 909
D +IAFTTRRY++
Sbjct: 338 DYLIAFTTRRYQD 300
>TC17093 weakly similar to UP|PYRH_LACPL (Q88VJ6) Uridylate kinase (UK)
(Uridine monophosphate kinase) (UMP kinase) , partial
(22%)
Length = 594
Score = 39.3 bits (90), Expect = 0.003
Identities = 24/66 (36%), Positives = 36/66 (54%)
Frame = +3
Query: 312 TDVDGVYSADPRKVSEAVILKTLSYQEAWEMSYFGANVLHPRTIIPVMRYGIPILIRNIF 371
T+VDGV+ DP++ EA +L TL+YQE +V+ I IP++ +F
Sbjct: 9 TNVDGVFDDDPKRNPEARLLDTLTYQEVITKD---LSVMDLTAITLCQENNIPVV---VF 170
Query: 372 NLSAPG 377
NL+ PG
Sbjct: 171 NLNKPG 188
>TC10932 homologue to UP|Q39889 (Q39889) Heat shock protein , partial (28%)
Length = 1001
Score = 34.7 bits (78), Expect = 0.072
Identities = 50/230 (21%), Positives = 89/230 (37%), Gaps = 16/230 (6%)
Frame = +3
Query: 721 YTHYFYEATVGAGLPIVST----LRGLLETGDKILQIEGIFSGTLSYIFNNFKDGRAFSE 776
+TH EA GA +S+ + L I GIF +S + RA
Sbjct: 216 FTHKTNEALAGAHELAMSSGHAQMTPLHLASTLISDPNGIFFQAISNSSGE-ESARAVER 392
Query: 777 VVGEAKEAGYTEPDPRDDLSGTDVARKVIILARESGLKLELSNIPIESLVPEPLR----- 831
V+ +A + ++ P D++ + K I A+ + +++ ++ L+ L
Sbjct: 393 VLNQALKKLPSQSPPPDEIPASTTLIKAIRRAQAAQKSRGDTHLAVDQLILGILEDSQIG 572
Query: 832 -----ACASAQEFMQQLPKFDQEFAKKQEDA--DNAGEVLRYVGVVDVTNKKGVVELRKY 884
A +A + +L K + KK E A D + L+ G D+ + G
Sbjct: 573 DLLKEAGVAAAKVKSELDKLRGKVGKKVESASGDTTFQALKTYGR-DLVEQAG------- 728
Query: 885 KKDHPFAQLSGSDNIIAFTTRRYKNQPLIVRGPGAGAQVTAGGIFSDILR 934
K D + ++ +RR KN P+++ PG G G+ I+R
Sbjct: 729 KLDPVIGRDEEIRRVVRILSRRTKNNPVLIGEPGVGKTAVVEGLAQRIVR 878
>BP055392
Length = 521
Score = 30.4 bits (67), Expect = 1.4
Identities = 17/44 (38%), Positives = 23/44 (51%), Gaps = 2/44 (4%)
Frame = -1
Query: 669 NTALVDCTADSIIAGH--YYEWLCKGIHVITPNKKANSGPLEQY 710
NT C+ + I GH Y W+C + P KKA+SG EQ+
Sbjct: 323 NTNSEKCSLED*IIGHPCYIIWICGSDTIGHPMKKASSGDDEQH 192
>TC12043 weakly similar to UP|Q9SND9 (Q9SND9) Anthranilate
N-hydroxycinnamoyl/benzoyltransferase-like protein,
partial (11%)
Length = 421
Score = 28.5 bits (62), Expect = 5.2
Identities = 15/34 (44%), Positives = 18/34 (52%), Gaps = 1/34 (2%)
Frame = -1
Query: 694 HVITPNKKANSGPLE-QYLRLRALQRQSYTHYFY 726
H P K N G L+ QY L+ LQ Q + HY Y
Sbjct: 241 HFSWPPKSQNEGTLQDQYYLLQPLQMQ*FYHYIY 140
>TC15287 similar to UP|Q9FMY5 (Q9FMY5) U2 snRNP auxiliary factor, small
subunit, partial (81%)
Length = 1263
Score = 28.5 bits (62), Expect = 5.2
Identities = 15/31 (48%), Positives = 19/31 (60%), Gaps = 1/31 (3%)
Frame = +2
Query: 530 NITVVIKRED-SIKALRAVHSRFYLSRTTIA 559
N+ V + ED + KAL A+H RFY R IA
Sbjct: 410 NVYVQFREEDEAAKALAALHGRFYSGRPIIA 502
>TC8944 similar to UP|Q9SIR2 (Q9SIR2) At2g25310 protein, partial (69%)
Length = 1150
Score = 28.5 bits (62), Expect = 5.2
Identities = 15/44 (34%), Positives = 24/44 (54%)
Frame = -1
Query: 364 PILIRNIFNLSAPGTKICHPVVSDYEDKSNLQNYVKGFATIDNL 407
PI++ +I ++ A G +I H +S Y LQN V G ++ L
Sbjct: 796 PIMVWDISSVPA*GNQIVHGFLSTYTPSCFLQNQVTGLLKMELL 665
>TC14147 similar to UP|ROC1_NICSY (Q08935) 29 kDa ribonucleoprotein A,
chloroplast precursor (CP29A), partial (63%)
Length = 1602
Score = 27.7 bits (60), Expect = 8.8
Identities = 16/39 (41%), Positives = 22/39 (56%), Gaps = 1/39 (2%)
Frame = -2
Query: 903 TTRRYKNQPLIVRGPGAGAQVTAGGI-FSDILRLASYLG 940
T R+ ++ L+VRG GAG + A G+ F LR Y G
Sbjct: 377 TERKVSDEDLVVRGEGAGLCIVAEGVLFLVELRGDGYTG 261
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.321 0.135 0.393
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,401,685
Number of Sequences: 28460
Number of extensions: 185714
Number of successful extensions: 835
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 828
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 832
length of query: 942
length of database: 4,897,600
effective HSP length: 99
effective length of query: 843
effective length of database: 2,080,060
effective search space: 1753490580
effective search space used: 1753490580
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 60 (27.7 bits)
Medicago: description of AC146585.12