BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146574.12 - phase: 0 /pseudo
(951 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP057010 258 2e-69
AV778252 234 4e-62
TC10326 similar to AAQ89612 (AAQ89612) At5g26850, partial (21%) 204 7e-53
TC19601 similar to UP|Q94KD1 (Q94KD1) At1g05960/T21E18_20, parti... 190 1e-48
AV777219 134 9e-32
TC8072 106 2e-23
TC17407 98 5e-21
TC17942 weakly similar to UP|Q94KD1 (Q94KD1) At1g05960/T21E18_20... 87 2e-17
CN825467 70 2e-13
TC11743 similar to UP|Q94KD1 (Q94KD1) At1g05960/T21E18_20, parti... 39 0.005
TC16867 33 0.28
TC19981 weakly similar to UP|AAP20228 (AAP20228) Resistance prot... 31 1.1
TC16108 weakly similar to UP|Q7XHN9 (Q7XHN9) Tetratricopeptide r... 31 1.1
TC8424 weakly similar to GB|BAC22265.1|24414015|AP003803 arm rep... 30 2.3
BI417934 29 4.0
TC10253 similar to UP|Q9LLM2 (Q9LLM2) MTD2, partial (28%) 29 4.0
TC15399 homologue to UP|Q39828 (Q39828) SDL5A, partial (28%) 29 4.0
TC8917 similar to UP|AAH64262 (AAH64262) MGC76273 protein, parti... 28 5.2
TC14163 28 6.8
TC12407 similar to UP|Q7XA68 (Q7XA68) At1g01640, partial (83%) 28 8.9
>BP057010
Length = 573
Score = 258 bits (660), Expect = 2e-69
Identities = 124/152 (81%), Positives = 136/152 (88%)
Frame = +2
Query: 1 MGVMSRRVVPACGNLCIFCPSLRARSRQPVKRYKKLIAEILPRNKVAELNDRKIGKLCEY 60
MGVMSRRVVP CGNLC CPSLRA SRQPVKRYKKL+A+I PRN+ AE NDRK+GKLC+Y
Sbjct: 116 MGVMSRRVVPVCGNLCCMCPSLRASSRQPVKRYKKLLADIFPRNQEAEPNDRKMGKLCDY 295
Query: 61 ASKNPLRIPKITENLEQRCYKDLRNESFGSVKVILCIYRKLLSSCREQIPLFASSLLGII 120
ASKNPLRIPK+T+NLEQ CYKDLRNE FGSVKV+LCIYRK LSSC+E +PLFA SLL II
Sbjct: 296 ASKNPLRIPKVTDNLEQICYKDLRNEVFGSVKVVLCIYRKFLSSCKEHMPLFAGSLLEII 475
Query: 121 RTLLEQTRADEVRILGCNTLVDFIIFQTDGTY 152
RTLLEQTR DE+RILGCNTL+DFI QTDGTY
Sbjct: 476 RTLLEQTRTDEIRILGCNTLIDFIDRQTDGTY 571
>AV778252
Length = 522
Score = 234 bits (598), Expect = 4e-62
Identities = 115/154 (74%), Positives = 135/154 (86%)
Frame = -2
Query: 346 DHKNVAKQPILQIDIINITTQVAQNVKQQASVAVIGAISDLIKHLRRCLQNSAEATDIGN 405
DHKNVAKQPILQI+IIN TT +A N KQQ SVA+ GAISDLIKHLR+CLQ S+EA+ IGN
Sbjct: 521 DHKNVAKQPILQINIINTTTPLAPNPKQQTSVAITGAISDLIKHLRKCLQYSSEASSIGN 342
Query: 406 DAHTLNTKLQSSIEMCILQLSNKVGDAGPIFDLMAVVLENVSSSTIVARTTISAVYQTAK 465
+ + LN +LQS++EMCILQLSNK+GD GPI DLMA VLEN+S++T +ARTTISA+YQTAK
Sbjct: 341 NGYKLNAELQSALEMCILQLSNKLGDVGPIPDLMAAVLENISNTTTIARTTISAIYQTAK 162
Query: 466 LITSVPNVLYHNKAFPDALFHQLLLAMAHPDRET 499
LITS+PNV Y KAFPDALFHQLL+AMAHPD +T
Sbjct: 161 LITSIPNVSYQKKAFPDALFHQLLMAMAHPDHDT 60
Score = 32.3 bits (72), Expect = 0.36
Identities = 21/57 (36%), Positives = 31/57 (53%), Gaps = 7/57 (12%)
Frame = -3
Query: 469 SVPNVLYHNK-------AFPDALFHQLLLAMAHPDRETQIGAHSILSMVLMPSVVSP 518
S P +++H K F + + L+L M +IGAHS+ S+VLMPS+ SP
Sbjct: 157 SHPFLMFHIKRRLSLMPCFINCSWQWLILTMT-----PRIGAHSVFSIVLMPSLFSP 2
>TC10326 similar to AAQ89612 (AAQ89612) At5g26850, partial (21%)
Length = 746
Score = 204 bits (518), Expect = 7e-53
Identities = 100/205 (48%), Positives = 141/205 (68%)
Frame = +2
Query: 1 MGVMSRRVVPACGNLCIFCPSLRARSRQPVKRYKKLIAEILPRNKVAELNDRKIGKLCEY 60
MG++SR++ PACGN+C+ CP+LR+ SRQPVKRYKKL+A+I P++ ++RKI KLCEY
Sbjct: 131 MGIISRKIFPACGNMCVCCPALRSSSRQPVKRYKKLLADIFPKSPDELPSERKIVKLCEY 310
Query: 61 ASKNPLRIPKITENLEQRCYKDLRNESFGSVKVILCIYRKLLSSCREQIPLFASSLLGII 120
A+KNP RIPKI + LE+RCYK+LR+ V +I + KLLS C+ QI FA +L +I
Sbjct: 311 AAKNPFRIPKIAKYLEERCYKELRSGHIKLVTIITESFNKLLSICKVQIAYFALDVLNVI 490
Query: 121 RTLLEQTRADEVRILGCNTLVDFIIFQTDGTYMFNLEGFIPKLCQLAQEVGDDERALLLR 180
LL ++ + ++ LGC +L FI Q D TY N+E + K+C L++E G+ LR
Sbjct: 491 TELLGYSKDETIQTLGCQSLTRFIFCQVDTTYTHNIEKLVRKVCILSREHGETHEKRCLR 670
Query: 181 SAGLQTLSSMVKFMGEHSHLSMDFD 205
++ LQ LS+MV FM E S++ DFD
Sbjct: 671 ASSLQCLSAMVWFMAEFSNIFADFD 745
>TC19601 similar to UP|Q94KD1 (Q94KD1) At1g05960/T21E18_20, partial (15%)
Length = 547
Score = 190 bits (482), Expect = 1e-48
Identities = 89/109 (81%), Positives = 98/109 (89%)
Frame = +2
Query: 1 MGVMSRRVVPACGNLCIFCPSLRARSRQPVKRYKKLIAEILPRNKVAELNDRKIGKLCEY 60
MGVMSRRVVP CGNLC CPSLRA SRQPVKRYKKL+A+I PRN+ AE NDRKIGKLC+Y
Sbjct: 221 MGVMSRRVVPVCGNLCCMCPSLRASSRQPVKRYKKLLADIFPRNQEAEPNDRKIGKLCDY 400
Query: 61 ASKNPLRIPKITENLEQRCYKDLRNESFGSVKVILCIYRKLLSSCREQI 109
ASKNPLRIPK+T+NLEQ CYKDLRNE FGSVKV+LCIYRK LSSC+E +
Sbjct: 401 ASKNPLRIPKVTDNLEQICYKDLRNEVFGSVKVVLCIYRKFLSSCKEHM 547
>AV777219
Length = 291
Score = 134 bits (336), Expect = 9e-32
Identities = 60/91 (65%), Positives = 74/91 (80%)
Frame = -2
Query: 1 MGVMSRRVVPACGNLCIFCPSLRARSRQPVKRYKKLIAEILPRNKVAELNDRKIGKLCEY 60
M V+SR + P CG+LC FCP+LR RSR P+KRYK L+A+I PR + E NDRKI KLCEY
Sbjct: 275 MSVISRNIWPVCGSLCFFCPALRERSRHPIKRYKHLLADIFPRTQEEEPNDRKISKLCEY 96
Query: 61 ASKNPLRIPKITENLEQRCYKDLRNESFGSV 91
ASK+PLR+PKIT LEQRCY++LRNE++ SV
Sbjct: 95 ASKDPLRVPKITSYLEQRCYRELRNENYQSV 3
>TC8072
Length = 723
Score = 106 bits (264), Expect = 2e-23
Identities = 63/101 (62%), Positives = 73/101 (71%), Gaps = 5/101 (4%)
Frame = +1
Query: 856 NSWNRLETGKQQKMLTIRSFKNQQ----ETKAIVLSSENE-EVSRQPVKALEYSKGDLKL 910
N L GK+ KM I SF++QQ ET+AIVLSSENE EVS P+K LEYS+GD+KL
Sbjct: 25 NQCEALVMGKRPKMSVIHSFRHQQQQQQETRAIVLSSENEIEVSPLPIKTLEYSEGDMKL 204
Query: 911 VTQEQFQAQDQIRFRSQDTRKQHSLRLPPSSPYDKFLKAAG 951
V QEQFQA R S D R+Q+S RLPP+SPYDKFLKAAG
Sbjct: 205 VCQEQFQA----RPCSYDYRQQNSFRLPPASPYDKFLKAAG 315
>TC17407
Length = 517
Score = 98.2 bits (243), Expect = 5e-21
Identities = 54/84 (64%), Positives = 64/84 (75%), Gaps = 1/84 (1%)
Frame = +3
Query: 869 MLTIRSFKNQQETKAIVLSSENE-EVSRQPVKALEYSKGDLKLVTQEQFQAQDQIRFRSQ 927
M I SFK+QQ+TKAIVLSS++E EV P KALEYSKGDLKLV+Q+ + QD + S
Sbjct: 3 MSVIHSFKHQQKTKAIVLSSKSEVEVPPLPFKALEYSKGDLKLVSQKPLKVQDPVPLLSH 182
Query: 928 DTRKQHSLRLPPSSPYDKFLKAAG 951
D + +SL LPPSSPYDKFLKAAG
Sbjct: 183 DHVQPNSLILPPSSPYDKFLKAAG 254
>TC17942 weakly similar to UP|Q94KD1 (Q94KD1) At1g05960/T21E18_20, partial
(8%)
Length = 523
Score = 86.7 bits (213), Expect = 2e-17
Identities = 40/68 (58%), Positives = 55/68 (80%), Gaps = 1/68 (1%)
Frame = +2
Query: 50 NDRKIGKLCEYASKNPLRIPKITENLEQRCYKDLRNESFGSVKVILCIYRKLLSSCREQI 109
NDRKIGKLC+YAS + L IP++ LEQRCYK+LRNE+ S K+++CIY+K L SC+EQ+
Sbjct: 2 NDRKIGKLCDYASPHLLPIPQLVNALEQRCYKELRNENLHSTKIVMCIYKKFLFSCKEQM 181
Query: 110 -PLFASSL 116
L+A++L
Sbjct: 182 FSLWANTL 205
>CN825467
Length = 740
Score = 69.7 bits (169), Expect(2) = 2e-13
Identities = 55/173 (31%), Positives = 87/173 (49%), Gaps = 1/173 (0%)
Frame = +1
Query: 636 TLIALLYYLLVLSYMALVRCFQLAFSLRSISLDQEGG-LPPSRRRSLLTLASHMLIFSAR 694
TLI L L ++R FQL SL ++ LD G LPP+ +RS+ L++ MLIF+ +
Sbjct: 181 TLIVLRMKNLKDRENLVIRFFQLPLSLWTLLLDPSNGTLPPACQRSIFVLSACMLIFACK 360
Query: 695 AADFSDLIPKVKASLTEAPVDPFLELVDDNLLRAVCIKSDKVVFGSVEDEVAAMKSLSAV 754
DL V SL + VDPFL + DD+ + A I D +GS D AM L +
Sbjct: 361 IYQIHDL-NDVFMSLAVSEVDPFLGISDDHQVYAK-IHMDVRDYGSAADNQLAMSILPEL 534
Query: 755 QLDDRQLKETVISYFMTKFSKLPEDELSSIKNQLLQGFSPDDAYPSGPPLFME 807
+ + +T+ + + + E + ++ QL + F PD+ + GP L ++
Sbjct: 535 RNRICKYHQTIKDVLVHNLAMIIELDAENLALQLSETFQPDEEFMFGPQLTLD 693
Score = 23.5 bits (49), Expect(2) = 2e-13
Identities = 10/21 (47%), Positives = 14/21 (66%)
Frame = +2
Query: 612 SCFLQSGFRQHLLKMALQIMR 632
SCFL SGF+ + LQI++
Sbjct: 95 SCFLPSGFKPIFQIICLQILK 157
>TC11743 similar to UP|Q94KD1 (Q94KD1) At1g05960/T21E18_20, partial (3%)
Length = 416
Score = 38.5 bits (88), Expect = 0.005
Identities = 14/19 (73%), Positives = 18/19 (94%)
Frame = +1
Query: 2 GVMSRRVVPACGNLCIFCP 20
GV+SR+V+PACG+LC FCP
Sbjct: 358 GVISRQVLPACGSLCFFCP 414
>TC16867
Length = 517
Score = 32.7 bits (73), Expect = 0.28
Identities = 17/50 (34%), Positives = 29/50 (58%)
Frame = +3
Query: 902 EYSKGDLKLVTQEQFQAQDQIRFRSQDTRKQHSLRLPPSSPYDKFLKAAG 951
++ L+ V + A + + +D R +++LPP+SP+D FLKAAG
Sbjct: 141 DHRNSALEKVANDDGYAPNPVAALPRDPRL--AVKLPPASPFDHFLKAAG 284
>TC19981 weakly similar to UP|AAP20228 (AAP20228) Resistance protein SlVe1
precursor, partial (9%)
Length = 403
Score = 30.8 bits (68), Expect = 1.1
Identities = 22/66 (33%), Positives = 31/66 (46%)
Frame = +1
Query: 733 SDKVVFGSVEDEVAAMKSLSAVQLDDRQLKETVISYFMTKFSKLPEDELSSIKNQLLQGF 792
S +FG ++ +A +KSLS +QL L TV F FS L L N L G+
Sbjct: 43 SSNNLFGPIDSSLADLKSLSILQLSHNNLSSTVPDSF-ANFSNLTTLHLI---NCSLNGY 210
Query: 793 SPDDAY 798
P + +
Sbjct: 211 FPKEIF 228
>TC16108 weakly similar to UP|Q7XHN9 (Q7XHN9) Tetratricopeptide
repeat(TPR)-containing protein-like, partial (3%)
Length = 597
Score = 30.8 bits (68), Expect = 1.1
Identities = 22/74 (29%), Positives = 32/74 (42%), Gaps = 2/74 (2%)
Frame = +3
Query: 511 LMPS--VVSPWLDQKKISKKVESDGLSIQHESLSGEDPLNGKPVEEKPIHHSLISMILVE 568
L PS V + W D I+ + G I S S +D + +H +S++
Sbjct: 102 LYPSLKVTTSWKDYPDIASTFTTTGSVIAALSSSDDD*IRS--------NHGQVSVVA** 257
Query: 569 LLCWWAFSNQLVKC 582
L C W+F N L KC
Sbjct: 258 LYCCWSFINMLEKC 299
>TC8424 weakly similar to GB|BAC22265.1|24414015|AP003803 arm repeat
containing protein homolog-like {Oryza sativa (japonica
cultivar-group);} , partial (49%)
Length = 1586
Score = 29.6 bits (65), Expect = 2.3
Identities = 30/108 (27%), Positives = 42/108 (38%), Gaps = 2/108 (1%)
Frame = -2
Query: 647 LSYMALVRCFQLAFSLRSISLDQEGGLPPSRRRS--LLTLASHMLIFSARAADFSDLIPK 704
L Y V LA + + GG+PPS RS LTL++H A F P
Sbjct: 1060 LKYRRRVSALCLARGVPDCERETRGGIPPSLTRSPLFLTLSTHSCRSVKTANSFPFTDPS 881
Query: 705 VKASLTEAPVDPFLELVDDNLLRAVCIKSDKVVFGSVEDEVAAMKSLS 752
AS T A + P + A + S + F + +A +S S
Sbjct: 880 SMAS-TRAAIPPSSTIASFPS*TAAKLLSTTIAFSARPVPCSATRSTS 740
>BI417934
Length = 502
Score = 28.9 bits (63), Expect = 4.0
Identities = 16/65 (24%), Positives = 32/65 (48%)
Frame = -2
Query: 406 DAHTLNTKLQSSIEMCILQLSNKVGDAGPIFDLMAVVLENVSSSTIVARTTISAVYQTAK 465
D H + +++ IL + ++GD GP+F L S+ + TT ++ +T+
Sbjct: 225 DRHGEQEDFLNGLDLAILHQTTELGDRGPLFLLSLFASARTRSTRSI--TTFASASETST 52
Query: 466 LITSV 470
L+ S+
Sbjct: 51 LLFSL 37
>TC10253 similar to UP|Q9LLM2 (Q9LLM2) MTD2, partial (28%)
Length = 531
Score = 28.9 bits (63), Expect = 4.0
Identities = 10/18 (55%), Positives = 14/18 (77%)
Frame = +1
Query: 567 VELLCWWAFSNQLVKCCY 584
V+LL W FS ++VKCC+
Sbjct: 244 VKLLIWGQFSKKIVKCCF 297
>TC15399 homologue to UP|Q39828 (Q39828) SDL5A, partial (28%)
Length = 801
Score = 28.9 bits (63), Expect = 4.0
Identities = 13/36 (36%), Positives = 20/36 (55%)
Frame = +3
Query: 752 SAVQLDDRQLKETVISYFMTKFSKLPEDELSSIKNQ 787
S V R+ K T++ YF T+ K+ LSS+ N+
Sbjct: 309 SIVYCQVREAKRTLLDYFFTELGKMDMKRLSSLLNE 416
>TC8917 similar to UP|AAH64262 (AAH64262) MGC76273 protein, partial (3%)
Length = 676
Score = 28.5 bits (62), Expect = 5.2
Identities = 18/48 (37%), Positives = 23/48 (47%)
Frame = +3
Query: 20 PSLRARSRQPVKRYKKLIAEILPRNKVAELNDRKIGKLCEYASKNPLR 67
P L+ R R+P+ + L A R K + N RK KL K PLR
Sbjct: 228 PMLKRRQRKPLMKITSL*APCSMRMKRRQRNLRKKYKLSPRKKKQPLR 371
>TC14163
Length = 1712
Score = 28.1 bits (61), Expect = 6.8
Identities = 21/71 (29%), Positives = 33/71 (45%), Gaps = 7/71 (9%)
Frame = +1
Query: 190 MVKFMGEHSHLSMDFDKIISAILENYVDLQSK-------SNLAKVEKLNSQSQNQLVQEF 242
+V F G + L DF+++ Y+DL ++A +EKLN S N L E
Sbjct: 1288 LVGFWGSGNKLKFDFERLKFGERFKYLDLSHNLVFGKVPKSVAGLEKLN-VSYNHLCGEI 1464
Query: 243 PKEEAHVSSML 253
PK + S+ +
Sbjct: 1465 PKTKFPASAFV 1497
>TC12407 similar to UP|Q7XA68 (Q7XA68) At1g01640, partial (83%)
Length = 943
Score = 27.7 bits (60), Expect = 8.9
Identities = 11/29 (37%), Positives = 19/29 (64%)
Frame = +3
Query: 26 SRQPVKRYKKLIAEILPRNKVAELNDRKI 54
++ P + +K I +LPRNK+ + DRK+
Sbjct: 426 AKDPTEVIRKAILRMLPRNKLRDDRDRKL 512
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.323 0.137 0.395
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,257,755
Number of Sequences: 28460
Number of extensions: 201462
Number of successful extensions: 1448
Number of sequences better than 10.0: 41
Number of HSP's better than 10.0 without gapping: 1432
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1444
length of query: 951
length of database: 4,897,600
effective HSP length: 99
effective length of query: 852
effective length of database: 2,080,060
effective search space: 1772211120
effective search space used: 1772211120
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 60 (27.7 bits)
Medicago: description of AC146574.12