BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146570.7 - phase: 0
(915 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC11041 weakly similar to UP|Q93ZL7 (Q93ZL7) At1g48280/F11A17_25... 181 6e-46
TC9442 similar to GB|BAC55960.1|28071265|AB087408 actin binding ... 172 2e-43
TC19330 similar to UP|Q96258 (Q96258) AR791, partial (11%) 135 4e-32
TC12050 weakly similar to GB|BAC55960.1|28071265|AB087408 actin ... 106 2e-23
TC12380 similar to GB|BAC55960.1|28071265|AB087408 actin binding... 87 9e-18
BP030165 83 2e-16
AV409602 61 7e-10
BP039505 53 2e-07
CB827128 38 0.006
BF177554 37 0.011
TC14338 similar to UP|Q9MAH5 (Q9MAH5) F12M16.16, partial (33%) 37 0.014
TC12500 similar to GB|AAH45354.1|28279536|BC045354 FNBP4 protein... 37 0.014
TC19458 weakly similar to UP|Q9STE8 (Q9STE8) Chloroplast import-... 36 0.031
AV419591 36 0.031
TC17867 similar to GB|AAM26654.1|20856224|AY101533 At1g14810/F10... 36 0.031
BP043764 35 0.041
TC14758 similar to UP|O24601 (O24601) Glycine-rich RNA binding p... 35 0.041
AV408970 35 0.053
BI420016 35 0.053
TC10343 similar to UP|Q9SZZ1 (Q9SZZ1) Fibrillarin-like protein (... 35 0.053
>TC11041 weakly similar to UP|Q93ZL7 (Q93ZL7) At1g48280/F11A17_25, partial
(86%)
Length = 920
Score = 181 bits (458), Expect = 6e-46
Identities = 82/171 (47%), Positives = 126/171 (72%)
Frame = +1
Query: 726 ELSFLSDERAVLKHFDWPEGKSDALREASFEYQDLMKLEKQVSNFTDDPKLPCEDALQKM 785
ELS L+DERAVLKHF WPE K+DA+REA+ EY++L LE+++S+F DD +PC +L+KM
Sbjct: 1 ELSTLADERAVLKHFKWPEKKADAMREAAVEYRELKMLEQEISSFKDDLDIPCGASLRKM 180
Query: 786 YSLLEKLEQSVYALLRTRDFAISRYKEFGVPVNWLLDSGVVGKIKLSSVQLANKYMKRIA 845
LL+K E+S+ L++ R AI Y+ + +P W+LDSG++ KIK +S+ L YMKR+
Sbjct: 181 ACLLDKSERSIQRLIKLRSSAIRSYQVYNIPTAWMLDSGMMSKIKQASMTLVKMYMKRLT 360
Query: 846 SEIDTLSGPENEPTREFLILQGVRFSFRVHQFAGGFDTESMKAFEELRNNI 896
E++++ + E +++ L+LQGV F+++ HQFAGG D+E++ AFEE+R +
Sbjct: 361 IELESIRNSDRESSQDSLLLQGVHFAYKAHQFAGGLDSETLCAFEEIRQRV 513
>TC9442 similar to GB|BAC55960.1|28071265|AB087408 actin binding protein
{Arabidopsis thaliana;} , partial (9%)
Length = 601
Score = 172 bits (436), Expect = 2e-43
Identities = 87/113 (76%), Positives = 97/113 (84%), Gaps = 1/113 (0%)
Frame = +3
Query: 804 DFAISRYKEFGVPVNWLLDSGVVGKIKLSSVQLANKYMKRIASEIDTLSGPENEPTREFL 863
D AISRYKEFG+PVNWLLDSGVVGKIKLSSVQLA KYMKR+ SE+D LSG E EP REFL
Sbjct: 3 DLAISRYKEFGIPVNWLLDSGVVGKIKLSSVQLAKKYMKRVTSELDALSGSEKEPAREFL 182
Query: 864 ILQGVRFSFRVHQFAGGFDTESMKAFEELRNNIHV-QAGEYNNKPEMATKDDQ 915
LQGVRF+FRVHQFAGGFD +SMKAFE+LRN I QAGE +NKPE A +D++
Sbjct: 183 TLQGVRFAFRVHQFAGGFDADSMKAFEDLRNRIQTPQAGE-DNKPETAGEDNE 338
>TC19330 similar to UP|Q96258 (Q96258) AR791, partial (11%)
Length = 507
Score = 135 bits (339), Expect = 4e-32
Identities = 81/183 (44%), Positives = 115/183 (62%), Gaps = 1/183 (0%)
Frame = +2
Query: 138 EVARNKIKELQRQIKIIANQTKGQLLLLKQKVSGLQAKEEEVVKKDAEIENNLKTVNDFE 197
+ A+ K+K L+R+I+ A Q K Q++ LKQ+VS LQ E + T +D E
Sbjct: 2 DAAKTKVKFLKRKIRHEAEQNKEQIINLKQRVSKLQDLESQA------------TASDQE 145
Query: 198 IEKKLKTVNDLEIEAVGLKRRNKELQHEKRELTVKLNAAESRITELSSVTENEMIADAKS 257
IE KL+ + DLE EA L++ N LQ + +L +L++ + L++ + ADA
Sbjct: 146 IETKLRRLKDLEAEAEQLRKTNLRLQMDNSDLARRLDSTQI----LANAVLEDPEADALR 313
Query: 258 ETG-RLRHANEDLQKQVEGLQMNRFSEVEELVYLRWVNACLRYELRNYKAPSGKSLARDL 316
E G RLR NE L K+VE LQ +R S+VEELVYLRW+NACLR+E+RNY+ P GK++ARDL
Sbjct: 314 EEGERLRLENEGLTKEVEQLQADRCSDVEELVYLRWINACLRHEMRNYQPPPGKTVARDL 493
Query: 317 NNS 319
+ S
Sbjct: 494 SKS 502
>TC12050 weakly similar to GB|BAC55960.1|28071265|AB087408 actin binding
protein {Arabidopsis thaliana;} , partial (7%)
Length = 492
Score = 106 bits (264), Expect = 2e-23
Identities = 45/76 (59%), Positives = 62/76 (81%)
Frame = +3
Query: 819 WLLDSGVVGKIKLSSVQLANKYMKRIASEIDTLSGPENEPTREFLILQGVRFSFRVHQFA 878
W+LD+G++GKIK+ SV+LA YMKR+A E+ S + +P ++++LQGVRF+FR+HQFA
Sbjct: 6 WMLDNGIIGKIKIGSVKLAXNYMKRVAMELQAKSALDKDPAMDYMLLQGVRFAFRIHQFA 185
Query: 879 GGFDTESMKAFEELRN 894
GGFD E+M AFEELRN
Sbjct: 186 GGFDAETMHAFEELRN 233
>TC12380 similar to GB|BAC55960.1|28071265|AB087408 actin binding protein
{Arabidopsis thaliana;} , partial (9%)
Length = 800
Score = 87.4 bits (215), Expect = 9e-18
Identities = 49/88 (55%), Positives = 63/88 (70%)
Frame = +1
Query: 1 MGSIMNLANDFDDEILSEFESLLSEQTDFPLLSEKTDDSKKHGGNETEMAKNGSDSEIEW 60
+ SI+N ANDF+D+IL EFE LLS + DFP + + D+ K ETEMA N + E+
Sbjct: 541 ISSIINRANDFEDDILPEFEDLLSGEIDFPFPTAEKDE--KDMVYETEMANNAN--ELGR 708
Query: 61 LRNVVEELEEREMKLQSELLEYYSLKEQ 88
LR +V+ELEERE+KL+ ELLEYY LKEQ
Sbjct: 709 LRQLVQELEEREVKLEGELLEYYGLKEQ 792
>BP030165
Length = 438
Score = 82.8 bits (203), Expect = 2e-16
Identities = 42/78 (53%), Positives = 58/78 (73%), Gaps = 3/78 (3%)
Frame = -2
Query: 832 SSVQLANKYMKRIASEIDTLSGPENEPTREFLILQGVRFSFRVHQFAGGFDTESMKAFEE 891
+SV+LA KYMKR+++E++T+ G P E LI+QGV+F+FRVHQFA GFD E+M+AF+E
Sbjct: 437 ASVKLAMKYMKRVSAELETVGGG---PEEEELIVQGVKFAFRVHQFASGFDVETMRAFQE 267
Query: 892 LR---NNIHVQAGEYNNK 906
LR N+ HVQ + K
Sbjct: 266 LRDKANSCHVQCHSHQQK 213
>AV409602
Length = 148
Score = 61.2 bits (147), Expect = 7e-10
Identities = 31/45 (68%), Positives = 36/45 (79%)
Frame = +1
Query: 92 IEEFQRQLRIKSVEIDMLHMTIKSLQEENNKLQEELIHEASAKRE 136
I E QRQL+IK+VEIDML++TI SLQ E KLQEEL H SAK+E
Sbjct: 13 IVELQRQLKIKTVEIDMLNITINSLQAERKKLQEELTHGGSAKKE 147
>BP039505
Length = 518
Score = 53.1 bits (126), Expect = 2e-07
Identities = 26/35 (74%), Positives = 27/35 (76%)
Frame = -2
Query: 794 QSVYALLRTRDFAISRYKEFGVPVNWLLDSGVVGK 828
QSVYALLR D ISRYKEFG PV LD+GVVGK
Sbjct: 490 QSVYALLRRSDLTISRYKEFGTPVKLQLDAGVVGK 386
>CB827128
Length = 556
Score = 38.1 bits (87), Expect = 0.006
Identities = 36/124 (29%), Positives = 59/124 (47%)
Frame = +1
Query: 118 EENNKLQEELIHEASAKRELEVARNKIKELQRQIKIIANQTKGQLLLLKQKVSGLQAKEE 177
E ++LQE L A + E + K+ L+ QIK+ Q + + + + SGL EE
Sbjct: 148 EVESQLQEALQRHAEKEAEAKDLNEKLNALEGQIKLHEEQAREAVAVSETHKSGL---EE 318
Query: 178 EVVKKDAEIENNLKTVNDFEIEKKLKTVNDLEIEAVGLKRRNKELQHEKRELTVKLNAAE 237
++K +L+TV D E++ KL E E GL N +L E KL+ +
Sbjct: 319 SLLKL-----KHLETVVD-ELQNKLL---HHEKETAGLNEENTKLNQEIAIYESKLSDLQ 471
Query: 238 SRIT 241
S+++
Sbjct: 472 SKLS 483
>BF177554
Length = 246
Score = 37.4 bits (85), Expect = 0.011
Identities = 18/46 (39%), Positives = 23/46 (49%)
Frame = -3
Query: 580 KIEKRPPRLPRPPPKPSDGAPVSNSLNEIPYAPSVPSPPPPPGSLP 625
K K PP PPP P P + L+ + + +P PPPPP LP
Sbjct: 196 KCHKAPPAKKNPPPPP----PQAEHLDHLAHHLPLPPPPPPPLPLP 71
>TC14338 similar to UP|Q9MAH5 (Q9MAH5) F12M16.16, partial (33%)
Length = 1751
Score = 37.0 bits (84), Expect = 0.014
Identities = 19/48 (39%), Positives = 25/48 (51%), Gaps = 8/48 (16%)
Frame = +3
Query: 585 PPRLPRPPPKPSDGAPVSN--------SLNEIPYAPSVPSPPPPPGSL 624
PP LP PP S A V + + + P APS P+PPPPP ++
Sbjct: 114 PPPLPPSPPSLSSAATVPSPPYPSPPAATSSPPPAPSPPAPPPPPSTI 257
>TC12500 similar to GB|AAH45354.1|28279536|BC045354 FNBP4 protein {Danio
rerio;} , partial (5%)
Length = 678
Score = 37.0 bits (84), Expect = 0.014
Identities = 23/55 (41%), Positives = 29/55 (51%), Gaps = 14/55 (25%)
Frame = +1
Query: 585 PPR----LPRPPPKPSDG--------APVSNSLNEI--PYAPSVPSPPPPPGSLP 625
PP+ LP PPP PS + VS S+ ++ P +PS P P PPP SLP
Sbjct: 61 PPKSLKALPPPPPPPSAPFIAGSAIISKVSKSVGDVSLPCSPSPPPPQPPPPSLP 225
Score = 32.3 bits (72), Expect = 0.35
Identities = 24/74 (32%), Positives = 32/74 (42%), Gaps = 20/74 (27%)
Frame = +1
Query: 572 TITEMKLFKIEKRPPRLPRPPPK------PSDGAPVSN--------------SLNEIPYA 611
TIT+ L PP+ P PPPK P P+S+ SL+ P
Sbjct: 466 TITQTPL----PSPPQPPSPPPKYGVATIPPPPLPMSSIDRTPLPLLSTPLTSLDAPPPT 633
Query: 612 PSVPSPPPPPGSLP 625
P++ PPPPP + P
Sbjct: 634 PTLSPPPPPPPTPP 675
Score = 31.2 bits (69), Expect = 0.77
Identities = 16/41 (39%), Positives = 21/41 (51%), Gaps = 4/41 (9%)
Frame = +1
Query: 585 PPRLPRPPPKPSD---GAPVSNSLNEIPYA-PSVPSPPPPP 621
PP LP P P+ G+ + + P + PS P PPPPP
Sbjct: 211 PPSLPPTTPPPASVIAGSAIMSGEASFPCSLPSPPQPPPPP 333
>TC19458 weakly similar to UP|Q9STE8 (Q9STE8) Chloroplast import-associated
channel homolog (AT3g46740/T6H20_230), partial (10%)
Length = 469
Score = 35.8 bits (81), Expect = 0.031
Identities = 17/41 (41%), Positives = 22/41 (53%)
Frame = -1
Query: 581 IEKRPPRLPRPPPKPSDGAPVSNSLNEIPYAPSVPSPPPPP 621
++++PP P PPP P+ P P P VP PPPPP
Sbjct: 310 LDQKPP-FPSPPPPPNQPPP--------PPPPVVPPPPPPP 215
>AV419591
Length = 276
Score = 35.8 bits (81), Expect = 0.031
Identities = 19/41 (46%), Positives = 21/41 (50%)
Frame = +2
Query: 584 RPPRLPRPPPKPSDGAPVSNSLNEIPYAPSVPSPPPPPGSL 624
+PP +PRPPP P PV S P VP PPP P L
Sbjct: 128 QPPVVPRPPPLPQPRLPVRCS-----QPPVVPRPPPLPQPL 235
>TC17867 similar to GB|AAM26654.1|20856224|AY101533 At1g14810/F10B6_6
{Arabidopsis thaliana;}, partial (13%)
Length = 331
Score = 35.8 bits (81), Expect = 0.031
Identities = 16/49 (32%), Positives = 24/49 (48%), Gaps = 4/49 (8%)
Frame = +2
Query: 591 PPPKPSDGAPVSNSLNEIPYAPSVPSPPPP----PGSLPRGAVGDDKVK 635
PPP PS +P ++ + PY+P+ SP P P S P A + +
Sbjct: 176 PPPSPSSASPAPSAKSSSPYSPTATSPTAPSRCSPPSAPPAAASHSRTR 322
>BP043764
Length = 479
Score = 35.4 bits (80), Expect = 0.041
Identities = 25/77 (32%), Positives = 29/77 (37%), Gaps = 18/77 (23%)
Frame = +3
Query: 567 DFDNQTITEMKLFKIEKRPPRLPRPPPKPSDG-APVSNSLNEIPYA-------------- 611
D +N + K PP P+P PKP AP SN L P+
Sbjct: 129 DSNNSAAPFRRPLKTTTTPPSNPKPTPKPKPKPAPTSNPLKN-PFKSSHALTNKLWLTSK 305
Query: 612 ---PSVPSPPPPPGSLP 625
P P PPPPP LP
Sbjct: 306 LSPPPPPPPPPPPPPLP 356
>TC14758 similar to UP|O24601 (O24601) Glycine-rich RNA binding protein 2,
partial (93%)
Length = 725
Score = 35.4 bits (80), Expect = 0.041
Identities = 25/89 (28%), Positives = 34/89 (38%), Gaps = 2/89 (2%)
Frame = -1
Query: 585 PPRLPRPPPKPSDGAPVSNSLNEIPYAPSVPSP--PPPPGSLPRGAVGDDKVKRAPELVE 642
PP P PPP P D P + P P P P PPPP PR + R +
Sbjct: 449 PPYPPPPPP*PRDPPPPPYPPSRRPPPPPYPPPPRPPPPPPPPRAWASFTVMLRPSSSLP 270
Query: 643 FYQSLMKREAKKDASLLTSSTSNAADTRS 671
F S+ A + + + + +RS
Sbjct: 269 FIPSIALLIASSEVKVTNPNPLDLPVSRS 183
Score = 28.9 bits (63), Expect = 3.8
Identities = 19/45 (42%), Positives = 19/45 (42%), Gaps = 4/45 (8%)
Frame = -1
Query: 585 PPRLP--RPPPKPSDGAPVSNSLNEIPYAPSV--PSPPPPPGSLP 625
PP LP PPP P PY PS P PPPPP P
Sbjct: 533 PPPLP*REPPPSP-------------PYPPSRRPP*PPPPPPPYP 438
>AV408970
Length = 195
Score = 35.0 bits (79), Expect = 0.053
Identities = 19/37 (51%), Positives = 22/37 (59%)
Frame = +2
Query: 589 PRPPPKPSDGAPVSNSLNEIPYAPSVPSPPPPPGSLP 625
P PPP PS A S+ P APS P+PPPPP + P
Sbjct: 98 PSPPP-PSPPATSSSP----PSAPSPPAPPPPPTTTP 193
>BI420016
Length = 559
Score = 35.0 bits (79), Expect = 0.053
Identities = 25/98 (25%), Positives = 41/98 (41%)
Frame = +2
Query: 589 PRPPPKPSDGAPVSNSLNEIPYAPSVPSPPPPPGSLPRGAVGDDKVKRAPELVEFYQSLM 648
P PP P+ AP+S S P P+ PSPPP P P + +P + + +
Sbjct: 272 PPSPPSPTPNAPLSPS--SPPSPPTPPSPPPSPAPNPTPSATGSTTPSSP--LTPTSASL 439
Query: 649 KREAKKDASLLTSSTSNAADTRSNVIAEIENRSSFLLA 686
+ + TS S A T + + + + +S L +
Sbjct: 440 SSPSSLFSPASTSPASTTATTTTTTMIRLRSPASKLFS 553
Score = 33.1 bits (74), Expect = 0.20
Identities = 16/39 (41%), Positives = 19/39 (48%)
Frame = +2
Query: 585 PPRLPRPPPKPSDGAPVSNSLNEIPYAPSVPSPPPPPGS 623
PP P P PS +P N+ PS P+PP PP S
Sbjct: 245 PPSSPTLTPPPSPPSPTPNAPLSPSSPPSPPTPPSPPPS 361
>TC10343 similar to UP|Q9SZZ1 (Q9SZZ1) Fibrillarin-like protein (Fibrillarin
2) (AtFib2), partial (85%)
Length = 1116
Score = 35.0 bits (79), Expect = 0.053
Identities = 20/59 (33%), Positives = 25/59 (41%), Gaps = 14/59 (23%)
Frame = -2
Query: 585 PPRLPRPPPKPSDGAPVSNSLNEIPYAPSVP--------------SPPPPPGSLPRGAV 629
PPR P PPP P P +L +P P +P +PPPPP L G +
Sbjct: 197 PPRPPLPPPPP---LPPPRALKGVPLPPPLPPPSPLPLPLSPLPLNPPPPPRPLGGGTI 30
Score = 34.7 bits (78), Expect = 0.070
Identities = 19/45 (42%), Positives = 22/45 (48%), Gaps = 4/45 (8%)
Frame = -2
Query: 585 PPRLPRPPPK----PSDGAPVSNSLNEIPYAPSVPSPPPPPGSLP 625
PP PRPPP+ P P +L +P P P PPP P LP
Sbjct: 218 PPLPPRPPPRPPLPPPPPLPPPRALKGVPLPP--PLPPPSPLPLP 90
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.310 0.128 0.345
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,288,719
Number of Sequences: 28460
Number of extensions: 174185
Number of successful extensions: 3609
Number of sequences better than 10.0: 304
Number of HSP's better than 10.0 without gapping: 2122
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3036
length of query: 915
length of database: 4,897,600
effective HSP length: 99
effective length of query: 816
effective length of database: 2,080,060
effective search space: 1697328960
effective search space used: 1697328960
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 60 (27.7 bits)
Medicago: description of AC146570.7