BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146569.8 - phase: 0
(166 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC15540 similar to UP|Q9NGS5 (Q9NGS5) Prespore protein MF12 (Fra... 35 0.009
AI967777 32 0.073
AV408295 31 0.096
AV418552 31 0.12
TC13350 weakly similar to UP|Q9LPJ0 (Q9LPJ0) F6N18.12, partial (... 30 0.16
AV418628 30 0.21
TC12569 UP|Q40221 (Q40221) Protein containing C-terminal RING-fi... 30 0.28
AV411760 30 0.28
TC14212 similar to UP|Q9FN61 (Q9FN61) Gb|AAF07369.1, partial (10%) 30 0.28
BP077155 29 0.36
CB827141 29 0.36
TC14994 similar to UP|O82469 (O82469) Protein phosphatase-2C , p... 29 0.47
BP031757 29 0.47
TC15418 weakly similar to UP|Q8GT65 (Q8GT65) Serpin-like protein... 28 0.62
AV772111 28 0.62
TC7970 similar to UP|ADK2_ARATH (Q9LZG0) Adenosine kinase 2 (AK... 28 0.62
BP085186 28 0.81
TC16402 similar to UP|RS7_AVIMR (Q9ZNS1) 40S ribosomal protein S... 28 0.81
AV424971 28 0.81
BP034655 28 1.1
>TC15540 similar to UP|Q9NGS5 (Q9NGS5) Prespore protein MF12 (Fragment),
partial (6%)
Length = 806
Score = 34.7 bits (78), Expect = 0.009
Identities = 29/84 (34%), Positives = 33/84 (38%), Gaps = 11/84 (13%)
Frame = -3
Query: 82 GSC-KCNNFNCHGKCNSCTKCESSKC--DGTNCVTI----CSFKCGNSKC----DGNKCN 130
GSC CN NC G C CES C G+ C I C + CG+ C C
Sbjct: 225 GSC*CCNCCNC-GSCCCGCSCESCCC*S*GSCCCCIWESCCCWTCGSCCCCCCWSWVSCG 49
Query: 131 GCTPKVTSPCFQRCPQWCTCSKCC 154
GC C C C+C CC
Sbjct: 48 GCC------C--SCCCCCSCGSCC 1
>AI967777
Length = 461
Score = 31.6 bits (70), Expect = 0.073
Identities = 17/43 (39%), Positives = 20/43 (45%), Gaps = 4/43 (9%)
Frame = -2
Query: 81 HGSCKCNNF----NCHGKCNSCTKCESSKCDGTNCVTICSFKC 119
HGSC C+NF + H C C +S DG C C F C
Sbjct: 226 HGSCSCSNFYTTCSLHCTCYFSKCCYASNKDGPQC--NCWFYC 104
>AV408295
Length = 426
Score = 31.2 bits (69), Expect = 0.096
Identities = 13/24 (54%), Positives = 20/24 (83%)
Frame = +3
Query: 51 EEVKKKTEAEKKKEKEEEKKKMIE 74
EE +KKTE EKK +EE+K++++E
Sbjct: 195 EEEEKKTEEEKKPAEEEKKEEVVE 266
Score = 30.0 bits (66), Expect = 0.21
Identities = 15/25 (60%), Positives = 17/25 (68%)
Frame = +3
Query: 50 TEEVKKKTEAEKKKEKEEEKKKMIE 74
TEE KK E EKK+E EE+KK E
Sbjct: 213 TEEEKKPAEEEKKEEVVEEEKKPAE 287
>AV418552
Length = 386
Score = 30.8 bits (68), Expect = 0.12
Identities = 19/64 (29%), Positives = 25/64 (38%)
Frame = -3
Query: 91 CHGKCNSCTKCESSKCDGTNCVTICSFKCGNSKCDGNKCNGCTPKVTSPCFQRCPQWCTC 150
C G C +C C S C +CV C C + C + + C S C C+C
Sbjct: 354 C*GSC*NCC-CGKSCC--WSCVC*CCVSCSCASCCWSCASCCCWSCVSCCCWSSVS-CSC 187
Query: 151 SKCC 154
CC
Sbjct: 186 ESCC 175
Score = 30.8 bits (68), Expect = 0.12
Identities = 21/95 (22%), Positives = 29/95 (30%), Gaps = 22/95 (23%)
Frame = -3
Query: 83 SCKCNNFNCHG-KCNSCTKCESSKCDGTNCVTICSFKCGNSKCDGN---KCNGCTPKVTS 138
+C C C C C C + C + C + C + C + C + C C S
Sbjct: 336 NCCCGKSCCWSCVC*CCVSCSCASCCWS-CASCCCWSCVSCCCWSSVSCSCESCCWSCAS 160
Query: 139 PCFQRCPQ------------------WCTCSKCCA 155
C C WC C CC+
Sbjct: 159 CCCWSCCCSCK*GRSCCWG*KYDSICWCCCISCCS 55
>TC13350 weakly similar to UP|Q9LPJ0 (Q9LPJ0) F6N18.12, partial (17%)
Length = 489
Score = 30.4 bits (67), Expect = 0.16
Identities = 13/36 (36%), Positives = 18/36 (49%), Gaps = 2/36 (5%)
Frame = +3
Query: 128 KCNGCTPKVTSPCFQRCPQWCTCSKCCAPYQ--PYC 161
KC GC +V S C C C++C A ++ P C
Sbjct: 57 KCRGCERRVASVVVLPCRHMCICAECDAHFRACPVC 164
>AV418628
Length = 309
Score = 30.0 bits (66), Expect = 0.21
Identities = 15/56 (26%), Positives = 23/56 (40%), Gaps = 6/56 (10%)
Frame = -2
Query: 84 CKCNNFNCHGKCNSC------TKCESSKCDGTNCVTICSFKCGNSKCDGNKCNGCT 133
C C C +C+SC + C S + G C C C C ++C+ C+
Sbjct: 161 CCCCQSCCPSRCSSCSSCCRPSSCRSGRRQGGRCRCRC---CCCQSCCPSRCSSCS 3
>TC12569 UP|Q40221 (Q40221) Protein containing C-terminal RING-finger,
complete
Length = 2092
Score = 29.6 bits (65), Expect = 0.28
Identities = 16/51 (31%), Positives = 27/51 (52%)
Frame = -2
Query: 23 CVTGDNVDIVCLANQLKKKFNNVTILTTEEVKKKTEAEKKKEKEEEKKKMI 73
C T CL N+ + VTIL T++ E E +EK+++KK+++
Sbjct: 210 CTTDQQHKETCLVNR*ESDDG*VTILNTQD----DERESYREKQKQKKRVV 70
>AV411760
Length = 420
Score = 29.6 bits (65), Expect = 0.28
Identities = 12/25 (48%), Positives = 20/25 (80%)
Frame = +1
Query: 50 TEEVKKKTEAEKKKEKEEEKKKMIE 74
++EVKKK ++++EK+E KKK+ E
Sbjct: 238 SKEVKKKKNVKEEEEKQETKKKVAE 312
>TC14212 similar to UP|Q9FN61 (Q9FN61) Gb|AAF07369.1, partial (10%)
Length = 613
Score = 29.6 bits (65), Expect = 0.28
Identities = 14/43 (32%), Positives = 22/43 (50%)
Frame = +3
Query: 65 KEEEKKKMIEACHAVLHGSCKCNNFNCHGKCNSCTKCESSKCD 107
+E E + ++ C + G KC+ C G N KC+ SKC+
Sbjct: 267 EETEAELKLKKCSGIGQGRVKCSLSKCEGSPN--*KCKMSKCE 389
Score = 27.7 bits (60), Expect = 1.1
Identities = 16/57 (28%), Positives = 23/57 (40%)
Frame = +3
Query: 69 KKKMIEACHAVLHGSCKCNNFNCHGKCNSCTKCESSKCDGTNCVTICSFKCGNSKCD 125
+ K +E C + C G KC SKC+G+ + KC SKC+
Sbjct: 234 ESKCLEFCFCTEETEAELKLKKCSGIGQGRVKCSLSKCEGSP-----N*KCKMSKCE 389
>BP077155
Length = 432
Score = 29.3 bits (64), Expect = 0.36
Identities = 12/36 (33%), Positives = 18/36 (49%), Gaps = 2/36 (5%)
Frame = -2
Query: 128 KCNGCTPKVTSPCFQRCPQWCTCSKCCAPYQ--PYC 161
+C GC +V S C C C++C A ++ P C
Sbjct: 278 ECKGCWKRVASVVVLPCRHLCVCTECDAQFRACPVC 171
>CB827141
Length = 513
Score = 29.3 bits (64), Expect = 0.36
Identities = 11/30 (36%), Positives = 18/30 (59%)
Frame = -3
Query: 79 VLHGSCKCNNFNCHGKCNSCTKCESSKCDG 108
V+HG + NC G+ + C + E+SK +G
Sbjct: 313 VMHGFSESGQMNCFGRSSWCLRSEASKPNG 224
>TC14994 similar to UP|O82469 (O82469) Protein phosphatase-2C , partial
(89%)
Length = 1672
Score = 28.9 bits (63), Expect = 0.47
Identities = 15/45 (33%), Positives = 24/45 (53%)
Frame = +3
Query: 44 NVTILTTEEVKKKTEAEKKKEKEEEKKKMIEACHAVLHGSCKCNN 88
N L E+ KKK + +KKK+K+++KKK + V C +
Sbjct: 138 NFNQLLQEKKKKKLKKKKKKKKKKKKKKKKKQRKMVAEAEIVCQH 272
Score = 26.2 bits (56), Expect = 3.1
Identities = 12/30 (40%), Positives = 21/30 (70%)
Frame = +3
Query: 42 FNNVTILTTEEVKKKTEAEKKKEKEEEKKK 71
FN +L ++ KK + +KKK+K+++KKK
Sbjct: 135 FNFNQLLQEKKKKKLKKKKKKKKKKKKKKK 224
Score = 24.6 bits (52), Expect = 9.0
Identities = 12/38 (31%), Positives = 22/38 (57%)
Frame = +3
Query: 34 LANQLKKKFNNVTILTTEEVKKKTEAEKKKEKEEEKKK 71
+ L FN + ++ KK + +KKK+K+++KKK
Sbjct: 117 ICRTLDFNFNQLLQEKKKKKLKKKKKKKKKKKKKKKKK 230
>BP031757
Length = 507
Score = 28.9 bits (63), Expect = 0.47
Identities = 12/21 (57%), Positives = 18/21 (85%)
Frame = +2
Query: 51 EEVKKKTEAEKKKEKEEEKKK 71
EE KKK ++ KKKE+EEE+++
Sbjct: 209 EEKKKKKKSRKKKEEEEEEEQ 271
Score = 26.2 bits (56), Expect = 3.1
Identities = 11/24 (45%), Positives = 18/24 (74%)
Frame = +2
Query: 51 EEVKKKTEAEKKKEKEEEKKKMIE 74
EE KKK + +KK++EEE+++ E
Sbjct: 206 EEEKKKKKKSRKKKEEEEEEEQEE 277
Score = 25.4 bits (54), Expect = 5.2
Identities = 11/25 (44%), Positives = 19/25 (76%)
Frame = +2
Query: 51 EEVKKKTEAEKKKEKEEEKKKMIEA 75
E+ KKK +KK+E+EEE+++ E+
Sbjct: 212 EKKKKKKSRKKKEEEEEEEQEEPES 286
>TC15418 weakly similar to UP|Q8GT65 (Q8GT65) Serpin-like protein
(Fragment), partial (44%)
Length = 602
Score = 28.5 bits (62), Expect = 0.62
Identities = 12/32 (37%), Positives = 17/32 (52%)
Frame = +2
Query: 127 NKCNGCTPKVTSPCFQRCPQWCTCSKCCAPYQ 158
N C+ P+ +PCF CP + +CS P Q
Sbjct: 62 NSCSRNIPRTRTPCFLPCP-FTSCSASSPPVQ 154
>AV772111
Length = 423
Score = 28.5 bits (62), Expect = 0.62
Identities = 21/82 (25%), Positives = 30/82 (35%), Gaps = 1/82 (1%)
Frame = -3
Query: 83 SCKCNNFNCHGKCNSCTKCESSKCDGTNCVTICSFKCGNSKCDGNKCNGCTPKVTSPCFQ 142
SC+C + C C+ C T+ CG C C+G P C +
Sbjct: 412 SCRCGSLKQDVPCYQDLTCDRK------CQTMRD--CGRHACKRRCCDGDCPPCAEICGR 257
Query: 143 RCPQWCTCSKCCAP-YQPYCKP 163
R C KC +P ++ C P
Sbjct: 256 RLR--CKSHKCPSPCHRGACAP 197
>TC7970 similar to UP|ADK2_ARATH (Q9LZG0) Adenosine kinase 2 (AK 2)
(Adenosine 5'-phosphotransferase 2) , partial (97%)
Length = 1482
Score = 28.5 bits (62), Expect = 0.62
Identities = 21/69 (30%), Positives = 25/69 (35%), Gaps = 4/69 (5%)
Frame = +1
Query: 92 HGKCNSCTKCESSKCDGTNCVTICSFKCGNSKCDGNKCN----GCTPKVTSPCFQRCPQW 147
H +C SC C K C TI + G K +C CT + W
Sbjct: 850 HTRCRSCVCC*GWKSHIVPCNTIA*REIG*HKWS-RRCICWRISCTVG*GEAY*RMRESW 1026
Query: 148 CTCSKCCAP 156
CSKCC P
Sbjct: 1027MLCSKCCHP 1053
>BP085186
Length = 388
Score = 28.1 bits (61), Expect = 0.81
Identities = 11/21 (52%), Positives = 19/21 (90%)
Frame = -2
Query: 51 EEVKKKTEAEKKKEKEEEKKK 71
+E+KKK + +KKK+K+++KKK
Sbjct: 267 KEMKKKKKKKKKKKKKKKKKK 205
Score = 27.3 bits (59), Expect = 1.4
Identities = 12/27 (44%), Positives = 20/27 (73%)
Frame = -2
Query: 45 VTILTTEEVKKKTEAEKKKEKEEEKKK 71
+T + E KKK + +KKK+K+++KKK
Sbjct: 282 ITRMGKEMKKKKKKKKKKKKKKKKKKK 202
Score = 25.8 bits (55), Expect = 4.0
Identities = 13/44 (29%), Positives = 26/44 (58%)
Frame = -2
Query: 31 IVCLANQLKKKFNNVTILTTEEVKKKTEAEKKKEKEEEKKKMIE 74
I + ++KKK ++ KKK + +KKK+K+ ++KK ++
Sbjct: 282 ITRMGKEMKKK-------KKKKKKKKKKKKKKKKKQNQRKKRMK 172
>TC16402 similar to UP|RS7_AVIMR (Q9ZNS1) 40S ribosomal protein S7, partial
(97%)
Length = 1044
Score = 28.1 bits (61), Expect = 0.81
Identities = 13/44 (29%), Positives = 17/44 (38%)
Frame = +2
Query: 115 CSFKCGNSKCDGNKCNGCTPKVTSPCFQRCPQWCTCSKCCAPYQ 158
C C C +K GC +P F CP C CC ++
Sbjct: 359 CCLYCH*KDCTTSKERGCCSATPNPHFNCCP-*CYAGGCCVSFR 487
>AV424971
Length = 416
Score = 28.1 bits (61), Expect = 0.81
Identities = 15/48 (31%), Positives = 17/48 (35%)
Frame = +3
Query: 108 GTNCVTICSFKCGNSKCDGNKCNGCTPKVTSPCFQRCPQWCTCSKCCA 155
G C +F S N + V CF C CT S CCA
Sbjct: 18 GCGCYKGRTFLFDGSFSKANVQSAYVASVRGSCFSNCLFSCTTSSCCA 161
>BP034655
Length = 517
Score = 27.7 bits (60), Expect = 1.1
Identities = 12/23 (52%), Positives = 19/23 (82%)
Frame = +2
Query: 49 TTEEVKKKTEAEKKKEKEEEKKK 71
TT + KKK + +KKK+K+++KKK
Sbjct: 137 TTMKRKKKKKKKKKKKKKKKKKK 205
Score = 26.9 bits (58), Expect = 1.8
Identities = 11/26 (42%), Positives = 20/26 (76%)
Frame = +2
Query: 46 TILTTEEVKKKTEAEKKKEKEEEKKK 71
T + ++ KKK + +KKK+K+++KKK
Sbjct: 137 TTMKRKKKKKKKKKKKKKKKKKKKKK 214
Score = 26.2 bits (56), Expect = 3.1
Identities = 11/26 (42%), Positives = 20/26 (76%)
Frame = +2
Query: 46 TILTTEEVKKKTEAEKKKEKEEEKKK 71
T+ ++ KKK + +KKK+K+++KKK
Sbjct: 140 TMKRKKKKKKKKKKKKKKKKKKKKKK 217
Score = 25.4 bits (54), Expect = 5.2
Identities = 10/18 (55%), Positives = 16/18 (88%)
Frame = +2
Query: 54 KKKTEAEKKKEKEEEKKK 71
KKK + +KKK+K+++KKK
Sbjct: 167 KKKKKKKKKKKKKKKKKK 220
Score = 24.6 bits (52), Expect = 9.0
Identities = 9/21 (42%), Positives = 18/21 (84%)
Frame = +2
Query: 54 KKKTEAEKKKEKEEEKKKMIE 74
KKK + +KKK+K+++ K+M++
Sbjct: 176 KKKKKKKKKKKKKKKTKRMMK 238
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.320 0.134 0.459
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,775,021
Number of Sequences: 28460
Number of extensions: 105552
Number of successful extensions: 1005
Number of sequences better than 10.0: 125
Number of HSP's better than 10.0 without gapping: 872
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 942
length of query: 166
length of database: 4,897,600
effective HSP length: 83
effective length of query: 83
effective length of database: 2,535,420
effective search space: 210439860
effective search space used: 210439860
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)
Medicago: description of AC146569.8