BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146568.7 - phase: 0
(703 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AW719682 301 2e-82
TC10578 similar to UP|CAC87839 (CAC87839) Cullin 3B (Fragment), ... 257 1e-71
TC9828 similar to UP|Q7Y1Y8 (Q7Y1Y8) Cullin 3a (Fragment), parti... 230 7e-61
AV779136 97 9e-21
TC19035 UP|Q84LL5 (Q84LL5) Cullin 4, partial (14%) 89 2e-18
TC15368 homologue to UP|Q84LL5 (Q84LL5) Cullin 4, partial (11%) 86 3e-17
AV425591 74 8e-14
TC15275 similar to UP|CAC87837 (CAC87837) Cullin 1C (Fragment), ... 40 0.001
TC10686 homologue to UP|Q8LP18 (Q8LP18) Cullin-like protein1, pa... 40 0.002
BP072051 31 0.59
TC15254 28 3.8
TC8006 similar to UP|Q9LVE9 (Q9LVE9) Genomic DNA, chromosome 3, ... 28 6.5
TC9581 UP|Q9SCA1 (Q9SCA1) Calcium-binding protein, complete 28 6.5
TC8429 weakly similar to UP|BAD03306 (BAD03306) MAP3K-like prote... 27 8.5
>AW719682
Length = 480
Score = 301 bits (771), Expect = 2e-82
Identities = 151/159 (94%), Positives = 154/159 (95%)
Frame = +1
Query: 277 EVMTLHIRESGKQLVTDPERLKDPVEFVQRLLDEKDKYDKIINQAFNNDKSFQNALNSSF 336
EVMT HIRESGKQLVTDPERLKDPVEFVQRLL EKDKYDKIIN AFNNDK FQNAL+SSF
Sbjct: 4 EVMTSHIRESGKQLVTDPERLKDPVEFVQRLLHEKDKYDKIINLAFNNDKLFQNALSSSF 183
Query: 337 EYFINLNPRSPEFISLFVDDKLRKGLKGVNEDDVEVTLDKVMMLFRYLQEKDVFEKYYKQ 396
EYFINLNPRSPEFISLFVDDKLRKGLKGV+EDD+E TLDKVMMLFRYLQEKDVFEKYYKQ
Sbjct: 184 EYFINLNPRSPEFISLFVDDKLRKGLKGVSEDDIETTLDKVMMLFRYLQEKDVFEKYYKQ 363
Query: 397 HLAKRLLSGKTVSDDAERSLIVKLKTECGYQFTSKLEGM 435
HLAKRLLSGKTVSDDAERSLIVKLKTECGYQFTSKLEGM
Sbjct: 364 HLAKRLLSGKTVSDDAERSLIVKLKTECGYQFTSKLEGM 480
>TC10578 similar to UP|CAC87839 (CAC87839) Cullin 3B (Fragment), partial
(29%)
Length = 590
Score = 257 bits (657), Expect(2) = 1e-71
Identities = 130/162 (80%), Positives = 144/162 (88%)
Frame = +3
Query: 542 FNNADKLSYKEIEQATEIPAPDLKRCLQSLALVKGRNVLRKEPMSKDVGEDDAFSVNDKF 601
FNNAD+L+ KEIEQAT IP DLKRCLQSLA VKG+NVLRKEPMSKD+ EDDAF ND+F
Sbjct: 42 FNNADRLTCKEIEQATAIPMSDLKRCLQSLACVKGKNVLRKEPMSKDIAEDDAFLFNDQF 221
Query: 602 SSKLYKVKIGTVVAQKESEPEKQETRQRVEEDRKPQIEAAIVRIMKSRRLLDHNNLIAEV 661
+SK +KVKIGTVVAQ+ESEPE ETRQRVEEDRKPQIEAAIVRIMKSRR LDHN+++AEV
Sbjct: 222 TSKFFKVKIGTVVAQRESEPENLETRQRVEEDRKPQIEAAIVRIMKSRRTLDHNHVVAEV 401
Query: 662 TKQLQLRFLANPTEVKKRIESLIERDFLERDDNDRKMYRYLA 703
TKQLQ RFL NP +KKRIESLIER+FLERD DR+MYRYLA
Sbjct: 402 TKQLQSRFLPNPVVIKKRIESLIEREFLERDKVDRQMYRYLA 527
Score = 30.4 bits (67), Expect(2) = 1e-71
Identities = 13/14 (92%), Positives = 14/14 (99%)
Frame = +1
Query: 528 ELNVSTYQMCVLML 541
ELNVST+QMCVLML
Sbjct: 1 ELNVSTFQMCVLML 42
>TC9828 similar to UP|Q7Y1Y8 (Q7Y1Y8) Cullin 3a (Fragment), partial (39%)
Length = 551
Score = 230 bits (586), Expect = 7e-61
Identities = 118/132 (89%), Positives = 125/132 (94%)
Frame = +3
Query: 572 ALVKGRNVLRKEPMSKDVGEDDAFSVNDKFSSKLYKVKIGTVVAQKESEPEKQETRQRVE 631
ALVKGRNVLRKEPM+KD+GEDDAF VNDKFSSKLYKVKIGTVVA KESEPEK ETR RVE
Sbjct: 3 ALVKGRNVLRKEPMTKDLGEDDAFFVNDKFSSKLYKVKIGTVVAPKESEPEKLETRPRVE 182
Query: 632 EDRKPQIEAAIVRIMKSRRLLDHNNLIAEVTKQLQLRFLANPTEVKKRIESLIERDFLER 691
EDRKPQIEAA+VRIMKSR+ LDHN+LIAEVTKQLQ RFLANPTEVKKRIESLIER+FLER
Sbjct: 183 EDRKPQIEAALVRIMKSRKQLDHNHLIAEVTKQLQSRFLANPTEVKKRIESLIEREFLER 362
Query: 692 DDNDRKMYRYLA 703
DD DRK+YRYLA
Sbjct: 363 DDVDRKLYRYLA 398
>AV779136
Length = 513
Score = 97.1 bits (240), Expect = 9e-21
Identities = 59/174 (33%), Positives = 98/174 (55%)
Frame = -1
Query: 504 RRLSWQTNMGFADLKATFGKGQKHELNVSTYQMCVLMLFNNADKLSYKEIEQATEIPAPD 563
R+L+W ++G ++ F + EL V+TYQ L+L N++D+LSY EI + D
Sbjct: 513 RKLTWIYSLGTCNVVGKFDP-KTVELVVTTYQASALLLCNSSDRLSYSEIMTQLNLTDDD 337
Query: 564 LKRCLQSLALVKGRNVLRKEPMSKDVGEDDAFSVNDKFSSKLYKVKIGTVVAQKESEPEK 623
+ R L SL+ K + +L +EP + + D F N KF+ KL ++KI EK
Sbjct: 336 VIRLLHSLSCAKYK-ILNQEPNTNTISNTDYFECNSKFTDKLRRIKIPLPPVD-----EK 175
Query: 624 QETRQRVEEDRKPQIEAAIVRIMKSRRLLDHNNLIAEVTKQLQLRFLANPTEVK 677
++ + V++DR+ I+A++VRIMKSR++L + L+ E +QL F + +K
Sbjct: 174 KKVIENVDKDRRYAIDASLVRIMKSRKVLGYRQLVMECVEQLGRMFKPDVKAIK 13
>TC19035 UP|Q84LL5 (Q84LL5) Cullin 4, partial (14%)
Length = 305
Score = 89.4 bits (220), Expect = 2e-18
Identities = 48/101 (47%), Positives = 65/101 (63%), Gaps = 3/101 (2%)
Frame = +2
Query: 399 AKRLLSGKTVSDDAERSLIVKLKTECGYQFTSKLEGMFTDMKTSQDTMQGFYAS---HPD 455
AKRLL GK+ S DAE+S+I KLKTECG QFT+KLEGMF D++ S++ + F S
Sbjct: 2 AKRLLLGKSASIDAEKSMISKLKTECGSQFTNKLEGMFKDIELSKEINESFKQSSQARTK 181
Query: 456 LGDGPTLTVQVLTTGSWPTQSSITCNLPVEISALCEKFRSY 496
L G ++V VLTTG WPT + LP E++ + F+ +
Sbjct: 182 LPSGIEMSVHVLTTGYWPTYPPMDVRLPHELNVYQDIFKEF 304
>TC15368 homologue to UP|Q84LL5 (Q84LL5) Cullin 4, partial (11%)
Length = 517
Score = 85.5 bits (210), Expect = 3e-17
Identities = 44/87 (50%), Positives = 64/87 (72%)
Frame = +1
Query: 617 KESEPEKQETRQRVEEDRKPQIEAAIVRIMKSRRLLDHNNLIAEVTKQLQLRFLANPTEV 676
KE+ T +RV +DR+ Q++AAIVRIMK+R++L H LI E+ +QL +F P ++
Sbjct: 4 KETVEVNTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQL--KFPIKPADL 177
Query: 677 KKRIESLIERDFLERDDNDRKMYRYLA 703
KKRIESLI+R++LERD N+ ++Y YLA
Sbjct: 178 KKRIESLIDREYLERDKNNPQIYNYLA 258
>AV425591
Length = 247
Score = 73.9 bits (180), Expect = 8e-14
Identities = 32/74 (43%), Positives = 52/74 (70%)
Frame = +3
Query: 376 KVMMLFRYLQEKDVFEKYYKQHLAKRLLSGKTVSDDAERSLIVKLKTECGYQFTSKLEGM 435
+V+ L Y+ +KD+F ++Y++ LA+RLL K+ +DD ERS++ KLK +CG QFT K+EGM
Sbjct: 3 EVVKLLAYIGDKDLFAEFYRKKLARRLLFDKSANDDHERSILSKLKQQCGQQFTVKMEGM 182
Query: 436 FTDMKTSQDTMQGF 449
D+ +++ F
Sbjct: 183 VNDLTMAKENQTNF 224
>TC15275 similar to UP|CAC87837 (CAC87837) Cullin 1C (Fragment), partial
(8%)
Length = 562
Score = 40.0 bits (92), Expect = 0.001
Identities = 17/28 (60%), Positives = 23/28 (81%)
Frame = +1
Query: 676 VKKRIESLIERDFLERDDNDRKMYRYLA 703
+KKRIE LI RD+LER+ ++ M+RYLA
Sbjct: 19 IKKRIEDLISRDYLEREKDNPNMFRYLA 102
>TC10686 homologue to UP|Q8LP18 (Q8LP18) Cullin-like protein1, partial (5%)
Length = 656
Score = 39.7 bits (91), Expect = 0.002
Identities = 16/28 (57%), Positives = 23/28 (82%)
Frame = +3
Query: 676 VKKRIESLIERDFLERDDNDRKMYRYLA 703
+KKRIE LI RD+LERD ++ +++YLA
Sbjct: 33 IKKRIEDLISRDYLERDKDNANLFKYLA 116
>BP072051
Length = 441
Score = 31.2 bits (69), Expect = 0.59
Identities = 16/37 (43%), Positives = 23/37 (61%)
Frame = +3
Query: 505 RLSWQTNMGFADLKATFGKGQKHELNVSTYQMCVLML 541
+ SW TN+G+ +KAT + + L +TY MCVL L
Sbjct: 270 KFSWVTNLGYKLVKATNRERPPYRLEPATY-MCVLRL 377
>TC15254
Length = 581
Score = 28.5 bits (62), Expect = 3.8
Identities = 19/76 (25%), Positives = 34/76 (44%), Gaps = 9/76 (11%)
Frame = +2
Query: 628 QRVEEDRKPQIEAAIVRIMKSRRLLDHNNLIAEVT---------KQLQLRFLANPTEVKK 678
Q+ +ED KP+++ A VR R L N + + T K+ + EV+K
Sbjct: 92 QKPDEDEKPKVQVATVRGQAKARSLTPNKAVVKQTSPRSVATKPKEENRKQAETAKEVRK 271
Query: 679 RIESLIERDFLERDDN 694
L +++ + DD+
Sbjct: 272 VFGGLFKQETIYIDDD 319
>TC8006 similar to UP|Q9LVE9 (Q9LVE9) Genomic DNA, chromosome 3, P1 clone:
MIL23, partial (27%)
Length = 1225
Score = 27.7 bits (60), Expect = 6.5
Identities = 17/50 (34%), Positives = 24/50 (48%)
Frame = +3
Query: 457 GDGPTLTVQVLTTGSWPTQSSITCNLPVEISALCEKFRSYYLGTHTGRRL 506
G PT + +GSW +S +TC L + C + R +L GRRL
Sbjct: 468 GLDPTRKWFLSRSGSWMARSGLTCTLDRRLR--CRRRRGRFLCRLFGRRL 611
>TC9581 UP|Q9SCA1 (Q9SCA1) Calcium-binding protein, complete
Length = 1049
Score = 27.7 bits (60), Expect = 6.5
Identities = 21/103 (20%), Positives = 53/103 (51%), Gaps = 3/103 (2%)
Frame = +3
Query: 182 ESQRFIECCDCGDYLKKAERRLNEEMDRVGHYMDPETEKKINKVVETQMIENHMLRLIHM 241
E +R + D + ++ LN+ ++ +G ++ +K++ +++E I+ + + +
Sbjct: 348 ELKRVFQMFDRNGDGRITKKELNDSLENLGIFIP---DKELTQMIER--IDVNGDGCVDI 512
Query: 242 ENSG-LVNMLCD--DKYEDLGRMYNLFRRVADGLLKIREVMTL 281
+ G L + D D+ ED+ +N+F + DG + + E+ T+
Sbjct: 513 DEFGELYQSIMDERDEEEDMREAFNVFDQNGDGFITVEELRTV 641
>TC8429 weakly similar to UP|BAD03306 (BAD03306) MAP3K-like protein kinase,
partial (55%)
Length = 1298
Score = 27.3 bits (59), Expect = 8.5
Identities = 16/41 (39%), Positives = 23/41 (56%)
Frame = +3
Query: 327 SFQNALNSSFEYFINLNPRSPEFISLFVDDKLRKGLKGVNE 367
+FQ ALN E + L E I++F+DD + G GVN+
Sbjct: 495 TFQPALNVLKEVRVFLVTHPTEIITIFIDDHVTSG-NGVNK 614
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.320 0.136 0.389
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,897,173
Number of Sequences: 28460
Number of extensions: 117007
Number of successful extensions: 545
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 540
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 542
length of query: 703
length of database: 4,897,600
effective HSP length: 97
effective length of query: 606
effective length of database: 2,136,980
effective search space: 1295009880
effective search space used: 1295009880
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 58 (26.9 bits)
Medicago: description of AC146568.7