BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146568.5 - phase: 0
(297 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC14167 similar to UP|O48642 (O48642) Class III acidic endochiti... 298 1e-81
TC19619 similar to UP|CHIA_CICAR (P36908) Acidic endochitinase p... 149 4e-37
TC15376 weakly similar to UP|Q7SYH2 (Q7SYH2) Cystatin domain fet... 89 1e-18
TC18525 weakly similar to UP|O48642 (O48642) Class III acidic en... 39 8e-04
TC8167 similar to PIR|JC7519|JC7519 subtilisin-like serine prote... 28 2.4
TC16982 weakly similar to GB|AAO64893.1|29029028|BT005958 At1g54... 28 2.4
BI419351 27 4.1
TC10323 weakly similar to PIR|T04762|T04762 chitinase homolog T1... 27 5.4
TC10986 similar to GB|AAL56097.1|18028661|AF334030 ORF91 {Helico... 26 7.1
BE122534 26 7.1
BP052027 26 9.2
>TC14167 similar to UP|O48642 (O48642) Class III acidic endochitinase
precursor , partial (88%)
Length = 1336
Score = 298 bits (762), Expect = 1e-81
Identities = 157/301 (52%), Positives = 202/301 (66%), Gaps = 9/301 (2%)
Frame = +1
Query: 6 KSTISFTFFSLLVLALANGSNA-GKIAIYWGQNGNEGTLAEACATGNYEYVIIAFLPTFG 64
+ SF SLL L+ + + A G IA+YWGQNG EGTL+ AC TGNYE V++AFL FG
Sbjct: 55 RQAFSFIILSLLALSFTSINAAKGGIAVYWGQNGGEGTLSTACDTGNYEIVLLAFLNQFG 234
Query: 65 DGQTPMINLAGHCDPYSNGCTGLSSDIKSCQAKGIKVLLSIGGGAGSYSIASTKDANSVA 124
G+TP N AGHC +S CT L DI++CQ KG+KV LSIGG G YS+ S +DA+ VA
Sbjct: 235 CGRTPSWNFAGHCGDWS-PCTKLQPDIENCQRKGVKVFLSIGGAIGPYSLCSEQDAHEVA 411
Query: 125 TYLWNNFLGGKSSSRPLGPAVLDGIDFDIEGGSSQHWGDLARYLKGF---NKKVYITAAP 181
YL+ NFL G+ PLG LDGIDFDIEGG++ +W LAR+L NK ++AAP
Sbjct: 412 NYLYKNFLSGQYG--PLGSVTLDGIDFDIEGGTNLYWDALARHLDALRHKNKYFSLSAAP 585
Query: 182 QCPFPDAWIGNALTTGLFDYVWVQFYNNPPCQYNP---DAFMNFEDAWKQWTSGI-PANK 237
QC PD ++ A+ TGLFDY+++QFYNNPPCQY+ DA + + +W WTS + P N
Sbjct: 586 QCFMPDYYLDTAIKTGLFDYLFIQFYNNPPCQYDTSRGDATLLLQ-SWAHWTSYVHPNNT 762
Query: 238 IFLGLPASPTAAGS-GFISADDLTSTVLPVIKGSSKYGGVMLWSRYDDVQSGYSSSIKSH 296
+++GLPAS AA S G+I DL S VLP IK + YGG+MLW R++DVQ+ YS+ K
Sbjct: 763 VYMGLPASTAAAPSGGYIPPQDLISKVLPYIKPTPNYGGIMLWDRFNDVQNNYSNQFKDL 942
Query: 297 V 297
V
Sbjct: 943 V 945
>TC19619 similar to UP|CHIA_CICAR (P36908) Acidic endochitinase precursor ,
partial (41%)
Length = 541
Score = 149 bits (377), Expect = 4e-37
Identities = 75/133 (56%), Positives = 93/133 (69%), Gaps = 1/133 (0%)
Frame = +1
Query: 166 RYLKGFNKKVYITAAPQCPFPDAWIGNALTTGLFDYVWVQFYNNPPCQYNPDAFMNFEDA 225
R LK N V + AAPQCP+PDA + +A+ TGLFD+VWVQFYNNP CQY+ N ++
Sbjct: 1 RALKQHN--VILAAAPQCPYPDAHLDSAIKTGLFDHVWVQFYNNPQCQYSRGNTNNLLNS 174
Query: 226 WKQWTSGIPANKIFLGLPASPTAAGS-GFISADDLTSTVLPVIKGSSKYGGVMLWSRYDD 284
WKQWT ++FLGLPAS AA S GF+ AD L S VLP +K S KYGGVMLW R++D
Sbjct: 175 WKQWTDVSEIKQVFLGLPASEGAAPSGGFVPADVLNSQVLPSVKSSPKYGGVMLWDRFND 354
Query: 285 VQSGYSSSIKSHV 297
+GYS++IK V
Sbjct: 355 GPNGYSTAIKGSV 393
>TC15376 weakly similar to UP|Q7SYH2 (Q7SYH2) Cystatin domain fetuin-like
protein, partial (6%)
Length = 582
Score = 88.6 bits (218), Expect = 1e-18
Identities = 62/161 (38%), Positives = 77/161 (47%), Gaps = 32/161 (19%)
Frame = +3
Query: 22 ANGSNAGKIAIYWGQNGNE-GTLAEACATGNYEYVIIAFLPTFGDGQTPMINLAGHC-DP 79
A+ G +++YWGQ NE GTL EAC TGNY VII L + G TP +NL HC
Sbjct: 93 ADDGGGGLVSVYWGQASNEEGTLLEACETGNYGIVIIEGLIVYNSGNTPTLNLRNHCGSD 272
Query: 80 YSNGCTGLSSDIKSCQAKGIKVLLSI-------------------------GGGAGSYSI 114
+ C+ L DI+ CQ K +KV+LSI + S S
Sbjct: 273 QQHLCSALQKDIEYCQQKNVKVILSILADDSVTPAPPPPPPPLSPPSPTPSPSPSPSPSS 452
Query: 115 AST--KDAN---SVATYLWNNFLGGKSSSRPLGPAVLDGID 150
+ST D N +A YL N+F GK PLG LDGID
Sbjct: 453 SSTLKDDVNVEKELADYLLNHFFSGKPG--PLGNVSLDGID 569
>TC18525 weakly similar to UP|O48642 (O48642) Class III acidic endochitinase
precursor , partial (9%)
Length = 324
Score = 39.3 bits (90), Expect = 8e-04
Identities = 17/28 (60%), Positives = 20/28 (70%)
Frame = -2
Query: 270 SSKYGGVMLWSRYDDVQSGYSSSIKSHV 297
+S YGGVM+W RY D SGYS+ IK V
Sbjct: 323 ASNYGGVMIWDRYYDKISGYSAEIKDSV 240
>TC8167 similar to PIR|JC7519|JC7519 subtilisin-like serine proteinase -
Arabidopsis thaliana {Arabidopsis
thaliana;} , partial (65%)
Length = 2760
Score = 27.7 bits (60), Expect = 2.4
Identities = 21/79 (26%), Positives = 38/79 (47%), Gaps = 7/79 (8%)
Frame = +3
Query: 41 GTLAEACATGNY--EYVIIAFLPTFGDGQTPMINLAGHCDPYSNGCTGLSSDIKSCQAKG 98
GT + A G++ ++ + G P +A + +S+GC +SDI + +
Sbjct: 738 GTHTASTAAGSHVGNASLLGYASGTARGMAPQARVATYKVCWSDGC--FASDILAGMDRA 911
Query: 99 IK-----VLLSIGGGAGSY 112
I+ + LS+GGG+G Y
Sbjct: 912 IRDGVDVLSLSLGGGSGPY 968
>TC16982 weakly similar to GB|AAO64893.1|29029028|BT005958 At1g54570
{Arabidopsis thaliana;}, partial (6%)
Length = 878
Score = 27.7 bits (60), Expect = 2.4
Identities = 18/54 (33%), Positives = 26/54 (47%)
Frame = -1
Query: 230 TSGIPANKIFLGLPASPTAAGSGFISADDLTSTVLPVIKGSSKYGGVMLWSRYD 283
+S IP+ + F L A+P S FISA S + + G +LW+R D
Sbjct: 365 SSSIPSQRFF-SLCAAPLLLSSRFISAGLPFSAIAETRSTDTAKRGFVLWNRTD 207
>BI419351
Length = 322
Score = 26.9 bits (58), Expect = 4.1
Identities = 14/49 (28%), Positives = 22/49 (44%)
Frame = +3
Query: 79 PYSNGCTGLSSDIKSCQAKGIKVLLSIGGGAGSYSIASTKDANSVATYL 127
P+S G TG + + G + GG A + T+D S+ TY+
Sbjct: 159 PFSTGSTGNDTSSSPTMSSG*GTWIKPGGTAAVCKWSKTRDIKSLDTYV 305
>TC10323 weakly similar to PIR|T04762|T04762 chitinase homolog T16H5.170 -
Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(17%)
Length = 703
Score = 26.6 bits (57), Expect = 5.4
Identities = 21/94 (22%), Positives = 35/94 (36%), Gaps = 21/94 (22%)
Frame = +2
Query: 215 NPDAFMNFEDAWKQWTS-GIPANKIFLGLP----------------ASPTAAGSGFISAD 257
NP + ++ +W G+P +K+ LGLP +P G G +
Sbjct: 47 NPRGQFSADEGVTKWIQLGVPQSKLALGLPFYGFKWSLSDPNQHRLFAPATKGLGQVKYK 226
Query: 258 DLTSTVLPVIKGSSKYGGVML----WSRYDDVQS 287
D+ + ++ S+ W YDD QS
Sbjct: 227 DIKNAGAQIVYNSTFVTNYCFKGTDWFGYDDTQS 328
>TC10986 similar to GB|AAL56097.1|18028661|AF334030 ORF91 {Helicoverpa zea
single nucleocapsid nucleopolyhedrovirus;}, partial
(15%)
Length = 655
Score = 26.2 bits (56), Expect = 7.1
Identities = 25/86 (29%), Positives = 33/86 (38%), Gaps = 3/86 (3%)
Frame = -2
Query: 79 PYSNGCTGLSSDIKSCQAKGIKVLLSIGGGAGSYSIASTKDANSVATYLWNNFLGGKSSS 138
PY N C S D +AK G GAG+ + A + N V + + G SS
Sbjct: 423 PYENACCAASEDGCGAEAK--------GPGAGASTGAPSPVPNPVCKF--SVVRGNTGSS 274
Query: 139 RPLGPAVLDGI---DFDIEGGSSQHW 161
GP + + EGG S W
Sbjct: 273 FL*GPKTWENLS*AQEAKEGGESDFW 196
>BE122534
Length = 358
Score = 26.2 bits (56), Expect = 7.1
Identities = 11/33 (33%), Positives = 22/33 (66%), Gaps = 2/33 (6%)
Frame = +1
Query: 88 SSDIKSCQA--KGIKVLLSIGGGAGSYSIASTK 118
S+ ++ C+ KG++ L+ +GGG G+++ A K
Sbjct: 37 SAVMEKCKGVFKGLETLVDVGGGTGTFAKAIAK 135
>BP052027
Length = 472
Score = 25.8 bits (55), Expect = 9.2
Identities = 15/50 (30%), Positives = 25/50 (50%), Gaps = 4/50 (8%)
Frame = -2
Query: 162 GDLAR----YLKGFNKKVYITAAPQCPFPDAWIGNALTTGLFDYVWVQFY 207
GDL R ++ F + + +T+ CP P +G +T+ F VW+ Y
Sbjct: 303 GDLTRNALLWIS*FGRYLSLTSNLICPSPLNLVGFQVTSTPFPRVWILSY 154
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.317 0.136 0.426
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,867,095
Number of Sequences: 28460
Number of extensions: 87778
Number of successful extensions: 384
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 376
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 378
length of query: 297
length of database: 4,897,600
effective HSP length: 90
effective length of query: 207
effective length of database: 2,336,200
effective search space: 483593400
effective search space used: 483593400
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 55 (25.8 bits)
Medicago: description of AC146568.5