BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146567.12 + phase: 0 /pseudo
(307 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AU252093 140 2e-34
TC10994 similar to UP|BAD08034 (BAD08034) Hydrolase, alpha/beta ... 76 8e-15
BP054751 70 6e-13
AV406816 42 1e-04
TC17520 similar to GB|AAO63388.1|28950929|BT005324 At5g19850 {Ar... 40 6e-04
TC16751 weakly similar to UP|Q9ZP87 (Q9ZP87) Epoxide hydrolase ... 36 0.007
BP067888 33 0.061
TC18837 weakly similar to UP|Q39856 (Q39856) Epoxide hydrolase ... 33 0.079
TC11862 similar to UP|Q39856 (Q39856) Epoxide hydrolase , parti... 32 0.10
TC9176 32 0.14
TC16547 similar to UP|O49857 (O49857) Epoxide hydrolase , parti... 32 0.18
TC9030 similar to GB|AAM63479.1|21554372|AY086477 phospholipase-... 31 0.23
TC7942 similar to UP|Q8LPE6 (Q8LPE6) Epoxide hydrolase (Fragmen... 30 0.39
TC7941 similar to UP|O49857 (O49857) Epoxide hydrolase , partia... 30 0.39
AV425969 28 2.5
BP057672 27 3.3
TC15968 27 5.7
AW719523 26 9.7
BP054939 26 9.7
AV413504 26 9.7
>AU252093
Length = 350
Score = 140 bits (354), Expect = 2e-34
Identities = 68/91 (74%), Positives = 78/91 (84%)
Frame = +1
Query: 12 FKVVAETDADSFPSFLPKDVNRIKDPFARTLATRIQRLPVSVKFSENPIMSSCVKPLVQN 71
F++VA+ FPSFLP++V RI+DPFAR LA RIQRLPV V F+ENPIMSSCVKPLVQ+
Sbjct: 85 FRIVADA---GFPSFLPREVERIEDPFARKLAMRIQRLPVPVSFAENPIMSSCVKPLVQS 255
Query: 72 KENPIVLLHGFDSSCLEWRYTYPLLEEAGIE 102
KE PIVLLHGFDSSCLEWRY +PLLEE+G E
Sbjct: 256 KEPPIVLLHGFDSSCLEWRYGHPLLEESGFE 348
>TC10994 similar to UP|BAD08034 (BAD08034) Hydrolase, alpha/beta fold
family-like, partial (34%)
Length = 644
Score = 75.9 bits (185), Expect = 8e-15
Identities = 41/108 (37%), Positives = 66/108 (60%)
Frame = +2
Query: 68 LVQNKENPIVLLHGFDSSCLEWRYTYPLLEEAGIETWAIDILGWGFSDLEKLPSCDVVSK 127
+VQ + +PIVL+HGF +S WRY P L + + +AID+LG+G+S+ + L D +
Sbjct: 299 VVQGEGSPIVLIHGFGASLFHWRYNIPELAKKH-KVYAIDLLGFGWSE-KALVDYDAMVW 472
Query: 128 REHFYQFWKSYIKKPMILVGPSLGSAVAIDFAINYPEAVEKLVLIDAS 175
++ F K +K+P +LVG SLG A+ A PE V +VL++++
Sbjct: 473 KDQVVDFMKEIVKEPAVLVGNSLGGFTALISATGLPELVNGVVLLNSA 616
>BP054751
Length = 553
Score = 69.7 bits (169), Expect = 6e-13
Identities = 39/63 (61%), Positives = 46/63 (72%)
Frame = -2
Query: 245 GGRTTA*SAISLLCNCTVNYLMQLYAKYLIVVTYLMLKDQILL*NSLRNLFRRIPRQ*IN 304
G + TA SAISL CN TVNYLMQ YAKY IV +L+ K+QIL N L NL R+ PR *+N
Sbjct: 552 GVKMTASSAISLQCNYTVNYLMQFYAKYPIVGIFLIWKNQILPSN*LLNL*RKRPRS*VN 373
Query: 305 MFH 307
+FH
Sbjct: 372 VFH 364
>AV406816
Length = 417
Score = 42.4 bits (98), Expect = 1e-04
Identities = 30/95 (31%), Positives = 38/95 (39%), Gaps = 1/95 (1%)
Frame = +1
Query: 73 ENPIVLLHGFDSSCLEWRYTYPLLEEAGIETWAIDILGWGFSDLEKLPSCDVVS-KREHF 131
+N +V LHGF WR+ L AG A D G+G SD P S
Sbjct: 103 QNVVVFLHGFPEIWYSWRHQMIALANAGFRAIAPDFRGYGLSDPPPEPENATFSVLLSDL 282
Query: 132 YQFWKSYIKKPMILVGPSLGSAVAIDFAINYPEAV 166
+ + LVG GS A F+I +PE V
Sbjct: 283 LAILDALALSKVFLVGKDFGSRPAYLFSILHPERV 387
>TC17520 similar to GB|AAO63388.1|28950929|BT005324 At5g19850 {Arabidopsis
thaliana;}, partial (43%)
Length = 688
Score = 39.7 bits (91), Expect = 6e-04
Identities = 28/111 (25%), Positives = 52/111 (46%), Gaps = 10/111 (9%)
Frame = +1
Query: 76 IVLLHGFDSSCLEWRYTYPLLEEAGIETWAIDILGWGFSDLEKLPSCDVVSKREHFYQF- 134
+VL+HGF ++ WR P L ++ ++ID++G+G+SD + FY F
Sbjct: 292 LVLVHGFGANSDHWRKNIPALAKSH-RVYSIDLIGYGYSDKPNPRQFG----DDSFYTFE 456
Query: 135 -WKS--------YIKKPMILVGPSLGSAVAIDFAINYPEAVEKLVLIDASV 176
W + IK + S+G V + A+ P+ ++L++ S+
Sbjct: 457 TWATQLNEFCVDVIKDEAFFICNSIGGVVGLQAAVLEPQICRGILLLNISL 609
>TC16751 weakly similar to UP|Q9ZP87 (Q9ZP87) Epoxide hydrolase , partial
(55%)
Length = 638
Score = 36.2 bits (82), Expect = 0.007
Identities = 29/104 (27%), Positives = 41/104 (38%), Gaps = 4/104 (3%)
Frame = +2
Query: 73 ENPIVLLHGFDSSCLEWRYTYPLLEEAGIETWAIDILGWGFS----DLEKLPSCDVVSKR 128
E +V LHGF WR+ + AG A D G+G S + EK D+V +
Sbjct: 92 EKTVVFLHGFPEIWYTWRHQMIAVANAGYRAIAFDFRGYGLSEHPAEPEKANLMDLVGET 271
Query: 129 EHFYQFWKSYIKKPMILVGPSLGSAVAIDFAINYPEAVEKLVLI 172
S ILVG G+ A +PE V ++ +
Sbjct: 272 VGLLD---SLGINKAILVGKDFGAIPGYLVAALHPEKVASVITL 394
>BP067888
Length = 534
Score = 33.1 bits (74), Expect = 0.061
Identities = 23/76 (30%), Positives = 34/76 (44%), Gaps = 7/76 (9%)
Frame = +3
Query: 47 QRLPVSVKFSENPIMSSCVKP------LVQNKENPIVL-LHGFDSSCLEWRYTYPLLEEA 99
Q P S + S PI + V+ + + + P+VL LHGF WR+ L
Sbjct: 24 QSSPHSTQSSMEPIQHTTVEANGIKMHVAEKGQGPVVLFLHGFPELWYTWRHQILSLSAL 203
Query: 100 GIETWAIDILGWGFSD 115
G A D+ G+G +D
Sbjct: 204 GYRAVAPDLRGYGDTD 251
>TC18837 weakly similar to UP|Q39856 (Q39856) Epoxide hydrolase , partial
(89%)
Length = 1100
Score = 32.7 bits (73), Expect = 0.079
Identities = 28/96 (29%), Positives = 39/96 (40%), Gaps = 1/96 (1%)
Frame = +1
Query: 76 IVLLHGFDSSCLEWRYTYPLLEEAGIETWAIDILGWGFSDL-EKLPSCDVVSKREHFYQF 134
I+ LHGF WR+ L G A D+ G+G +D S +
Sbjct: 70 ILFLHGFPELWYSWRHQLLSLSALGYRAIAPDLRGYGDTDAPTDSSSYSALHIVGDLVGL 249
Query: 135 WKSYIKKPMILVGPSLGSAVAIDFAINYPEAVEKLV 170
+ + LVG G+A+A FA+ PE V LV
Sbjct: 250 LDALAVDRVFLVGHDWGAAMAWYFALFKPERVIALV 357
>TC11862 similar to UP|Q39856 (Q39856) Epoxide hydrolase , partial (50%)
Length = 564
Score = 32.3 bits (72), Expect = 0.10
Identities = 20/55 (36%), Positives = 26/55 (46%), Gaps = 1/55 (1%)
Frame = +1
Query: 68 LVQNKENPIVL-LHGFDSSCLEWRYTYPLLEEAGIETWAIDILGWGFSDLEKLPS 121
+ + E P+VL LHGF WR+ L G A D+ G+G D E PS
Sbjct: 58 VAEKGEGPVVLFLHGFPELWYSWRHQILALSSQGYRAVAPDLRGYG--DTEAPPS 216
>TC9176
Length = 671
Score = 32.0 bits (71), Expect = 0.14
Identities = 27/113 (23%), Positives = 47/113 (40%), Gaps = 5/113 (4%)
Frame = +1
Query: 68 LVQNKENPIVL-LHGFDSSCLEWRYTYPLLEEAGIETWAIDILGWGFSDL----EKLPSC 122
+ + + P+VL +HGF WR+ L G A D+ G+G +D+ S
Sbjct: 103 IAEKGQGPLVLFIHGFPDLSYSWRHQITALASLGYRCVAPDLRGYGDTDVPAAQTSYTSL 282
Query: 123 DVVSKREHFYQFWKSYIKKPMILVGPSLGSAVAIDFAINYPEAVEKLVLIDAS 175
VV +K + +VG G+ A + PE V+ +V++ +
Sbjct: 283 HVVGDLIRLLDVIAPDQEK-VFVVGHDWGALTAWSLSQYRPERVKAMVILSVA 438
>TC16547 similar to UP|O49857 (O49857) Epoxide hydrolase , partial (50%)
Length = 550
Score = 31.6 bits (70), Expect = 0.18
Identities = 16/49 (32%), Positives = 24/49 (48%), Gaps = 1/49 (2%)
Frame = +1
Query: 68 LVQNKENPIVL-LHGFDSSCLEWRYTYPLLEEAGIETWAIDILGWGFSD 115
+ + + P+VL LHGF WR+ L G A D+ G+G +D
Sbjct: 94 IAEKGQGPVVLFLHGFPELWYSWRHQILTLSSRGYHAVAPDLRGYGDTD 240
>TC9030 similar to GB|AAM63479.1|21554372|AY086477 phospholipase-like
protein {Arabidopsis thaliana;}, partial (53%)
Length = 596
Score = 31.2 bits (69), Expect = 0.23
Identities = 28/88 (31%), Positives = 41/88 (45%), Gaps = 7/88 (7%)
Frame = +3
Query: 76 IVLLHGFDSSCLEW--RYTYPLLEEAGIETWAIDILGWGFSD--LEKLPSCD-VVSKREH 130
+ ++HGF W + T +AG T AID G GFSD + +P + VV
Sbjct: 231 LAVVHGFTGES-SWFIQLTAVHFAKAGFATCAIDHQGHGFSDGLVAHIPDVNPVVDDGIS 407
Query: 131 FYQFWKSYI--KKPMILVGPSLGSAVAI 156
F+ ++S P L SLG A+A+
Sbjct: 408 FFNSFRSRFDPSLPSFLYSESLGGAIAL 491
>TC7942 similar to UP|Q8LPE6 (Q8LPE6) Epoxide hydrolase (Fragment) ,
partial (91%)
Length = 1288
Score = 30.4 bits (67), Expect = 0.39
Identities = 16/46 (34%), Positives = 22/46 (47%), Gaps = 1/46 (2%)
Frame = +3
Query: 68 LVQNKENPIVL-LHGFDSSCLEWRYTYPLLEEAGIETWAIDILGWG 112
+ + E P+VL LHGF WR+ L G A D+ G+G
Sbjct: 48 VAEKGEGPVVLFLHGFPELWYSWRHQILSLSSRGYRAVAPDLRGYG 185
>TC7941 similar to UP|O49857 (O49857) Epoxide hydrolase , partial (48%)
Length = 610
Score = 30.4 bits (67), Expect = 0.39
Identities = 16/46 (34%), Positives = 22/46 (47%), Gaps = 1/46 (2%)
Frame = +2
Query: 68 LVQNKENPIVL-LHGFDSSCLEWRYTYPLLEEAGIETWAIDILGWG 112
+ + E P+VL LHGF WR+ L G A D+ G+G
Sbjct: 164 VAEKGEGPVVLFLHGFPELWYSWRHQILSLSSRGYRAVAPDLRGYG 301
>AV425969
Length = 426
Score = 27.7 bits (60), Expect = 2.5
Identities = 21/92 (22%), Positives = 39/92 (41%), Gaps = 8/92 (8%)
Frame = +3
Query: 89 WRYTYPLLEEAGIETWAIDILGWGFSDLEKLPSC--------DVVSKREHFYQFWKSYIK 140
WR+ L + G A D+ G+G SD P+ D++ +HF +
Sbjct: 18 WRHQLNYLAQNGYHAVAPDLRGYGDSDSPLDPNSYTVQHIVGDLIGLLDHFGE------- 176
Query: 141 KPMILVGPSLGSAVAIDFAINYPEAVEKLVLI 172
+ +VG LG+ + + + P+ V+ V +
Sbjct: 177 QQAFVVGSDLGANIGWNLCLFRPDRVKGFVAL 272
>BP057672
Length = 439
Score = 27.3 bits (59), Expect = 3.3
Identities = 17/40 (42%), Positives = 23/40 (57%)
Frame = +3
Query: 65 VKPLVQNKENPIVLLHGFDSSCLEWRYTYPLLEEAGIETW 104
VKP + KE+ + +HG D L + TY LLE G +TW
Sbjct: 15 VKP--KQKED*VYSIHGLD---L*YIITYFLLENCGHDTW 119
>TC15968
Length = 689
Score = 26.6 bits (57), Expect = 5.7
Identities = 25/104 (24%), Positives = 46/104 (44%), Gaps = 4/104 (3%)
Frame = +2
Query: 76 IVLLHGFDSSCLEWRYTYPLLEEAGIETWAIDILGWGFSDLEKLPSCDVVSKREHFYQFW 135
IVL HGF + W++ P L D +G G ++ E + S E +
Sbjct: 140 IVLAHGFGTDQSLWKHFVPYLTN-DFRVILYDNMGAGTTNPEYF-DFERHSSLEGYAYDL 313
Query: 136 KSYIKK----PMILVGPSLGSAVAIDFAINYPEAVEKLVLIDAS 175
S +++ I VG S+ + + +I+ P+ K++++ AS
Sbjct: 314 LSILEELRVDSCIFVGHSVSAMIGAVASISRPDLFSKIIMVGAS 445
>AW719523
Length = 543
Score = 25.8 bits (55), Expect = 9.7
Identities = 11/23 (47%), Positives = 16/23 (68%)
Frame = +3
Query: 26 FLPKDVNRIKDPFARTLATRIQR 48
F P+D+N++K RTL+T I R
Sbjct: 384 FPPRDLNQLKISILRTLSTGINR 452
>BP054939
Length = 514
Score = 25.8 bits (55), Expect = 9.7
Identities = 15/43 (34%), Positives = 23/43 (52%), Gaps = 4/43 (9%)
Frame = +2
Query: 245 GGRTTA*SAISLL----CNCTVNYLMQLYAKYLIVVTYLMLKD 283
GGR A A + C+C +NY ++ AKY +V ++L D
Sbjct: 221 GGRVLACDA*EVTTVQCCHCQINYWERVAAKYWLVEGKIVLFD 349
>AV413504
Length = 314
Score = 25.8 bits (55), Expect = 9.7
Identities = 11/31 (35%), Positives = 14/31 (44%)
Frame = +2
Query: 111 WGFSDLEKLPSCDVVSKREHFYQFWKSYIKK 141
W +S L SCD R F W S++ K
Sbjct: 116 WKWSTLRYAKSCDAYCTRSCFIARWVSFVPK 208
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.335 0.145 0.460
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,203,394
Number of Sequences: 28460
Number of extensions: 73281
Number of successful extensions: 575
Number of sequences better than 10.0: 40
Number of HSP's better than 10.0 without gapping: 569
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 569
length of query: 307
length of database: 4,897,600
effective HSP length: 90
effective length of query: 217
effective length of database: 2,336,200
effective search space: 506955400
effective search space used: 506955400
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.6 bits)
S2: 55 (25.8 bits)
Medicago: description of AC146567.12