BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146567.1 - phase: 0
(178 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC14068 homologue to UP|Q9SLR7 (Q9SLR7) Thiamin biosynthetic enz... 29 0.53
TC8676 similar to UP|O48587 (O48587) DREG-2 like protein, partia... 28 0.69
TC15207 similar to UP|Q9FGP9 (Q9FGP9) Gb|AAF31706.1 (AT5g22790/K... 27 1.5
TC7815 similar to UP|Q9AVB5 (Q9AVB5) Plasma membrane intrinsic p... 27 2.6
BP085152 26 3.4
AV767344 26 3.4
TC18875 similar to UP|CTNS_ARATH (P57758) Cystinosin homolog, pa... 26 3.4
AV412250 26 4.5
CN825784 25 5.9
TC14281 similar to UP|Q9XHC6 (Q9XHC6) Cytochrome P450 monooxygen... 25 5.9
AW163904 25 7.7
TC17839 25 7.7
TC14429 weakly similar to UP|Q8LEJ6 (Q8LEJ6) Arabinogalactan pro... 25 7.7
>TC14068 homologue to UP|Q9SLR7 (Q9SLR7) Thiamin biosynthetic enzyme,
partial (85%)
Length = 1494
Score = 28.9 bits (63), Expect = 0.53
Identities = 31/114 (27%), Positives = 54/114 (47%), Gaps = 2/114 (1%)
Frame = -3
Query: 21 VSLINLAISLFALVAIESSS--QSLGRTYAFLLFCSILLDISWFILFTHEIWNISSDDYA 78
+S+ + +SLF VA + S Q L T A + S+ +SWF+ ++ +++
Sbjct: 838 LSIKPMLLSLFTPVAPKGPSWPQELTTTLASMTLGSMQDWVSWFMETKAQL--VTTPPTL 665
Query: 79 KFFIFSVKLTLAMQIIGFIVRLSSSLLWIQIYRLGASYVDTASRAADFDLRSSF 132
FF ++K + A + F + L+SSLL I + A + T + SSF
Sbjct: 664 PFF--TIKSSAATALKSFTLGLASSLLMIVEVKRAACLITT*LSCSS*STPSSF 509
>TC8676 similar to UP|O48587 (O48587) DREG-2 like protein, partial (95%)
Length = 1346
Score = 28.5 bits (62), Expect = 0.69
Identities = 16/42 (38%), Positives = 22/42 (52%)
Frame = -2
Query: 59 ISWFILFTHEIWNISSDDYAKFFIFSVKLTLAMQIIGFIVRL 100
+SW I+F H I N+ S FF F++ L +I F V L
Sbjct: 901 LSWLIVFPHAIANMQS-----FFRFNISSPLKCNLINFWVWL 791
>TC15207 similar to UP|Q9FGP9 (Q9FGP9) Gb|AAF31706.1 (AT5g22790/K8E10_2),
partial (55%)
Length = 1195
Score = 27.3 bits (59), Expect = 1.5
Identities = 12/23 (52%), Positives = 16/23 (69%)
Frame = +3
Query: 42 SLGRTYAFLLFCSILLDISWFIL 64
+L RTY +LLFC +LL F+L
Sbjct: 507 NLKRTYLYLLFCKVLLSGVSFLL 575
>TC7815 similar to UP|Q9AVB5 (Q9AVB5) Plasma membrane intrinsic protein
2-1, complete
Length = 1450
Score = 26.6 bits (57), Expect = 2.6
Identities = 12/36 (33%), Positives = 21/36 (58%)
Frame = -2
Query: 45 RTYAFLLFCSILLDISWFILFTHEIWNISSDDYAKF 80
R + L SIL + WF+L+TH + +++ A+F
Sbjct: 105 RVFTLFLNLSILRHL-WFVLYTHSLGTVAARARAEF 1
>BP085152
Length = 401
Score = 26.2 bits (56), Expect = 3.4
Identities = 12/20 (60%), Positives = 13/20 (65%)
Frame = -3
Query: 121 SRAADFDLRSSFLSPVTPAV 140
S A F LRSS LSP+ P V
Sbjct: 87 SAAGSFPLRSSSLSPINPTV 28
>AV767344
Length = 485
Score = 26.2 bits (56), Expect = 3.4
Identities = 7/20 (35%), Positives = 15/20 (75%)
Frame = -1
Query: 49 FLLFCSILLDISWFILFTHE 68
F+L C+++L + W ++F H+
Sbjct: 461 FVLCCNLVLSLIWVVVFAHD 402
>TC18875 similar to UP|CTNS_ARATH (P57758) Cystinosin homolog, partial (58%)
Length = 567
Score = 26.2 bits (56), Expect = 3.4
Identities = 23/63 (36%), Positives = 29/63 (45%), Gaps = 10/63 (15%)
Frame = +1
Query: 27 AISLFALVAIESSSQ-----SLGRTY-----AFLLFCSILLDISWFILFTHEIWNISSDD 76
AI+LF +V E SQ S+G + A + F L D SW L + N SS D
Sbjct: 364 AITLFQIVIYERGSQKVSKISIGIVFVAWLTAAICFFIALSDHSWLWLLSIFNSNSSSYD 543
Query: 77 YAK 79
Y K
Sbjct: 544 YCK 552
>AV412250
Length = 403
Score = 25.8 bits (55), Expect = 4.5
Identities = 20/58 (34%), Positives = 26/58 (44%)
Frame = +3
Query: 107 IQIYRLGASYVDTASRAADFDLRSSFLSPVTPAVARQISNSNEILGGSIYDPAYYSSL 164
I +YR S T S A F+ + P IS N +LGGS+Y Y+ SL
Sbjct: 108 ITVYRQIISAAFTLSLAFIFERNNM---PNLTFKLLSISFLNGLLGGSLYQNLYFESL 272
>CN825784
Length = 548
Score = 25.4 bits (54), Expect = 5.9
Identities = 12/44 (27%), Positives = 22/44 (49%)
Frame = -3
Query: 23 LINLAISLFALVAIESSSQSLGRTYAFLLFCSILLDISWFILFT 66
L+++ + + A I + + + +LL C LL + W LFT
Sbjct: 534 LVDVVVMVVAEFCIHAVATGTWYHFIWLLQCCFLL*LGWIALFT 403
>TC14281 similar to UP|Q9XHC6 (Q9XHC6) Cytochrome P450 monooxygenaseCYP93D1,
partial (97%)
Length = 1768
Score = 25.4 bits (54), Expect = 5.9
Identities = 12/38 (31%), Positives = 22/38 (57%)
Frame = +2
Query: 46 TYAFLLFCSILLDISWFILFTHEIWNISSDDYAKFFIF 83
T F+ ++LD+ ++LFTH + NI + +F+F
Sbjct: 1655 TVFFIRLWFVILDV--YLLFTHCLRNIQTTIKKSYFVF 1762
>AW163904
Length = 416
Score = 25.0 bits (53), Expect = 7.7
Identities = 11/21 (52%), Positives = 15/21 (71%)
Frame = -3
Query: 36 IESSSQSLGRTYAFLLFCSIL 56
+ S+S S+ R+ FLL CSIL
Sbjct: 192 LRSTSSSMARSLTFLL*CSIL 130
>TC17839
Length = 615
Score = 25.0 bits (53), Expect = 7.7
Identities = 19/46 (41%), Positives = 21/46 (45%)
Frame = -3
Query: 22 SLINLAISLFALVAIESSSQSLGRTYAFLLFCSILLDISWFILFTH 67
+L +LAI F L S S T F CS LLD S F F H
Sbjct: 577 AL*DLAIGQFPLFNHSFPSASCFDTSPF*EECSFLLDDSEFFCFFH 440
>TC14429 weakly similar to UP|Q8LEJ6 (Q8LEJ6) Arabinogalactan protein-like,
partial (19%)
Length = 530
Score = 25.0 bits (53), Expect = 7.7
Identities = 19/68 (27%), Positives = 33/68 (47%)
Frame = +2
Query: 14 LGASYNGVSLINLAISLFALVAIESSSQSLGRTYAFLLFCSILLDISWFILFTHEIWNIS 73
LGA+++ S+I L+ +LF+ ++ L F ILL + WF F H ++
Sbjct: 329 LGAAFSVFSVI-LSCTLFS--------------FSVLFFIYILLSVCWF--FVHWLYEKD 457
Query: 74 SDDYAKFF 81
+ Y F+
Sbjct: 458 LNPYLCFY 481
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.324 0.137 0.397
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,186,947
Number of Sequences: 28460
Number of extensions: 43150
Number of successful extensions: 302
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 302
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 302
length of query: 178
length of database: 4,897,600
effective HSP length: 84
effective length of query: 94
effective length of database: 2,506,960
effective search space: 235654240
effective search space used: 235654240
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 52 (24.6 bits)
Medicago: description of AC146567.1