BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146566.1 + phase: 0
(155 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC18461 similar to UP|BAC99019 (BAC99019) Makorin ring-zinc-fing... 59 2e-11
AV773558 34 0.013
TC16950 similar to UP|RGP1_HUMAN (P46060) Ran GTPase-activating ... 32 0.037
TC11009 similar to GB|AAP21377.1|30102918|BT006569 At1g47970 {Ar... 30 0.24
TC11195 UP|Q9FJH4 (Q9FJH4) Similarity to ring finger protein, pa... 29 0.32
BP034655 29 0.41
AU251519 29 0.41
TC13318 similar to UP|CAA20314 (CAA20314) SPBC30B4.01c protein (... 29 0.41
TC17157 similar to UP|CAD27462 (CAD27462) Nucleosome assembly pr... 28 0.54
TC12685 weakly similar to UP|Q7QA92 (Q7QA92) AgCP13961 (Fragment... 28 0.54
AV416122 28 0.70
BP054131 28 0.70
BP038231 28 0.70
TC10643 similar to UP|BAC84847 (BAC84847) Acidic nuclear phospho... 28 0.70
TC12959 similar to UP|AAS52042 (AAS52042) ADR122Cp, partial (6%) 27 1.2
TC17687 weakly similar to GB|AAH05628.1|13542865|BC005628 acidic... 27 1.6
CB826779 27 1.6
BI420041 27 1.6
TC18955 similar to UP|Q9LU67 (Q9LU67) Phosphatidylserine decarbo... 27 1.6
TC8507 similar to UP|Q8LJS2 (Q8LJS2) Nucleolar histone deacetyla... 27 1.6
>TC18461 similar to UP|BAC99019 (BAC99019) Makorin ring-zinc-finger protein
(Fragment), partial (6%)
Length = 655
Score = 58.5 bits (140), Expect(2) = 2e-11
Identities = 39/107 (36%), Positives = 52/107 (48%), Gaps = 2/107 (1%)
Frame = +2
Query: 23 RDSDFFEGMDPFEMMALSDSLAGGSGPCLGPFDSDDDDDDGFRVSRMAAMQEAMAIGMDA 82
RDS+F + MD EMMALS P +G + SD++D
Sbjct: 2 RDSEFVDEMDACEMMALS--------PSMGLYHSDEED---------------------- 91
Query: 83 FGPDDDEEEFNVFRMAAMQEAMVSGIDDFGPDDFPG--IDPMDAALI 127
E+N F+MAA EAM SG+DDFG +DF +PM+AAL+
Sbjct: 92 --------EYNFFQMAAFSEAMASGLDDFGHEDFENEEFNPMEAALL 208
Score = 24.6 bits (52), Expect(2) = 2e-11
Identities = 7/15 (46%), Positives = 14/15 (92%)
Frame = +1
Query: 134 HMDDEEDDEDYEDEE 148
HM++EE+D+D +D++
Sbjct: 229 HMEEEEEDDDDDDDD 273
>AV773558
Length = 469
Score = 33.9 bits (76), Expect = 0.013
Identities = 11/19 (57%), Positives = 18/19 (93%)
Frame = -2
Query: 136 DDEEDDEDYEDEEEYTDDE 154
DD++DD+D +D+++YTDDE
Sbjct: 363 DDDDDDDDDDDDDDYTDDE 307
Score = 26.6 bits (57), Expect = 2.0
Identities = 8/14 (57%), Positives = 13/14 (92%)
Frame = -2
Query: 136 DDEEDDEDYEDEEE 149
DD++DD+DY D+E+
Sbjct: 345 DDDDDDDDYTDDED 304
>TC16950 similar to UP|RGP1_HUMAN (P46060) Ran GTPase-activating protein 1,
partial (3%)
Length = 643
Score = 32.3 bits (72), Expect = 0.037
Identities = 16/42 (38%), Positives = 22/42 (52%)
Frame = +1
Query: 113 PDDFPGIDPMDAALISMMMHSHMDDEEDDEDYEDEEEYTDDE 154
PD FP PM L + +DEE++E E+EEE D+
Sbjct: 262 PDIFPQFSPMKTPLPPPIADPPEEDEEEEEKKEEEEEEDPDK 387
>TC11009 similar to GB|AAP21377.1|30102918|BT006569 At1g47970 {Arabidopsis
thaliana;}, partial (44%)
Length = 700
Score = 29.6 bits (65), Expect = 0.24
Identities = 29/101 (28%), Positives = 38/101 (36%)
Frame = +2
Query: 55 DSDDDDDDGFRVSRMAAMQEAMAIGMDAFGPDDDEEEFNVFRMAAMQEAMVSGIDDFGPD 114
D D+DDDD +A G D +D+EEE V E G D D
Sbjct: 59 DGDEDDDD-----------DAPGGGDDDDDEEDEEEEGGV-------EGGRGGGGDPDDD 184
Query: 115 DFPGIDPMDAALISMMMHSHMDDEEDDEDYEDEEEYTDDEY 155
D DD++DD++ E+EEE EY
Sbjct: 185 D--------------------DDDDDDDEEEEEEEDLGTEY 247
Score = 27.7 bits (60), Expect = 0.92
Identities = 35/120 (29%), Positives = 43/120 (35%), Gaps = 16/120 (13%)
Frame = +2
Query: 45 GGSGPCLGPFDSDDDDDDGFRVSRMAAMQEAMAIGMDAFGPDDDEE--------EFNVFR 96
GG G P D DDDDDD DD+EE E+ V R
Sbjct: 149 GGRGGGGDPDDDDDDDDD-----------------------DDEEEEEEEDLGTEYLVRR 259
Query: 97 MAAMQEAMVSGIDDFGP-----DDFPGIDPMDAALISMMMHSHMD---DEEDDEDYEDEE 148
A E + DF P DD D A + S S D D++ D+ ED+E
Sbjct: 260 TVAAAEDEEAS-SDFEPEEDEGDDNDNDDGEKAGVPSKRKRSDKDGSGDDDSDDGGEDDE 436
Score = 25.0 bits (53), Expect = 5.9
Identities = 9/18 (50%), Positives = 14/18 (77%)
Frame = +2
Query: 137 DEEDDEDYEDEEEYTDDE 154
DE+DD+D ED++ DD+
Sbjct: 26 DEDDDDDGEDDDGDEDDD 79
>TC11195 UP|Q9FJH4 (Q9FJH4) Similarity to ring finger protein, partial (7%)
Length = 594
Score = 29.3 bits (64), Expect = 0.32
Identities = 19/54 (35%), Positives = 29/54 (53%)
Frame = +3
Query: 38 ALSDSLAGGSGPCLGPFDSDDDDDDGFRVSRMAAMQEAMAIGMDAFGPDDDEEE 91
+L+DS+ G G LG F DDD DD F + + + +A + F D D+E+
Sbjct: 300 SLTDSVFDGLGLDLG-FAPDDDTDDFFPAAGSGTLDD-LAAQIAGFVSDSDDED 455
>BP034655
Length = 517
Score = 28.9 bits (63), Expect = 0.41
Identities = 10/20 (50%), Positives = 17/20 (85%)
Frame = +1
Query: 136 DDEEDDEDYEDEEEYTDDEY 155
++EE++ED ED+EE D++Y
Sbjct: 199 EEEEEEEDEEDDEEDEDEDY 258
Score = 27.3 bits (59), Expect = 1.2
Identities = 10/19 (52%), Positives = 16/19 (83%)
Frame = +1
Query: 136 DDEEDDEDYEDEEEYTDDE 154
DDEE++E+ E+EEE ++E
Sbjct: 139 DDEEEEEEEEEEEEEEEEE 195
Score = 26.2 bits (56), Expect = 2.7
Identities = 9/19 (47%), Positives = 16/19 (83%)
Frame = +1
Query: 136 DDEEDDEDYEDEEEYTDDE 154
DD+E++E+ E+EEE ++E
Sbjct: 136 DDDEEEEEEEEEEEEEEEE 192
Score = 25.8 bits (55), Expect = 3.5
Identities = 9/19 (47%), Positives = 16/19 (83%)
Frame = +1
Query: 136 DDEEDDEDYEDEEEYTDDE 154
D+EE++E+ E+EEE ++E
Sbjct: 142 DEEEEEEEEEEEEEEEEEE 198
Score = 25.8 bits (55), Expect = 3.5
Identities = 9/19 (47%), Positives = 16/19 (83%)
Frame = +1
Query: 136 DDEEDDEDYEDEEEYTDDE 154
++EE++E+ EDEE+ +DE
Sbjct: 190 EEEEEEEEEEDEEDDEEDE 246
Score = 25.8 bits (55), Expect = 3.5
Identities = 9/19 (47%), Positives = 16/19 (83%)
Frame = +1
Query: 136 DDEEDDEDYEDEEEYTDDE 154
++EE++E+ E+EEE D+E
Sbjct: 172 EEEEEEEEEEEEEEEEDEE 228
Score = 25.8 bits (55), Expect = 3.5
Identities = 9/19 (47%), Positives = 16/19 (83%)
Frame = +1
Query: 136 DDEEDDEDYEDEEEYTDDE 154
++EE++E+ E+EEE +DE
Sbjct: 169 EEEEEEEEEEEEEEEEEDE 225
>AU251519
Length = 412
Score = 28.9 bits (63), Expect = 0.41
Identities = 15/43 (34%), Positives = 21/43 (47%)
Frame = +2
Query: 45 GGSGPCLGPFDSDDDDDDGFRVSRMAAMQEAMAIGMDAFGPDD 87
GGS G D D DD+ +R + +AMQ + +D DD
Sbjct: 260 GGSDSDSGGDDDDSDDERSYRAGKHSAMQVPLKKRLDLTERDD 388
>TC13318 similar to UP|CAA20314 (CAA20314) SPBC30B4.01c protein (SPBC3D6.14C
protein) (Fragment), partial (15%)
Length = 543
Score = 28.9 bits (63), Expect = 0.41
Identities = 10/19 (52%), Positives = 16/19 (83%)
Frame = +3
Query: 136 DDEEDDEDYEDEEEYTDDE 154
D+EEDDED +D+++ DD+
Sbjct: 270 DEEEDDEDEDDDDDDDDDD 326
Score = 28.5 bits (62), Expect = 0.54
Identities = 10/19 (52%), Positives = 16/19 (83%)
Frame = +3
Query: 136 DDEEDDEDYEDEEEYTDDE 154
DD++DD+D E+EE+ DD+
Sbjct: 306 DDDDDDDDGEEEEDDDDDD 362
Score = 27.7 bits (60), Expect = 0.92
Identities = 10/19 (52%), Positives = 15/19 (78%)
Frame = +3
Query: 136 DDEEDDEDYEDEEEYTDDE 154
DD++DD+D +D EE DD+
Sbjct: 297 DDDDDDDDDDDGEEEEDDD 353
Score = 27.7 bits (60), Expect = 0.92
Identities = 21/93 (22%), Positives = 44/93 (46%), Gaps = 4/93 (4%)
Frame = +3
Query: 66 VSRMAAMQEAMAIGMDAFGPDD----DEEEFNVFRMAAMQEAMVSGIDDFGPDDFPGIDP 121
++ +AAM+ A I F DD + ++R+ + + + + P+ +P P
Sbjct: 63 LASVAAMKSAPII---TFSSDDVVVKSPNDLRLYRLIELHNGLQAWLVH-DPEIYPDGPP 230
Query: 122 MDAALISMMMHSHMDDEEDDEDYEDEEEYTDDE 154
+ + ++EEDD++ ED+E+ DD+
Sbjct: 231 KP-------VQTDNEEEEDDDEEEDDEDEDDDD 308
Score = 27.7 bits (60), Expect = 0.92
Identities = 10/19 (52%), Positives = 15/19 (78%)
Frame = +3
Query: 136 DDEEDDEDYEDEEEYTDDE 154
DD++DD+D + EEE DD+
Sbjct: 300 DDDDDDDDDDGEEEEDDDD 356
Score = 26.9 bits (58), Expect = 1.6
Identities = 10/19 (52%), Positives = 15/19 (78%)
Frame = +3
Query: 136 DDEEDDEDYEDEEEYTDDE 154
DD++DD+D +EEE DD+
Sbjct: 303 DDDDDDDDDGEEEEDDDDD 359
Score = 26.9 bits (58), Expect = 1.6
Identities = 9/19 (47%), Positives = 16/19 (83%)
Frame = +3
Query: 136 DDEEDDEDYEDEEEYTDDE 154
DDEE+D++ ED+++ DD+
Sbjct: 267 DDEEEDDEDEDDDDDDDDD 323
Score = 26.2 bits (56), Expect = 2.7
Identities = 9/19 (47%), Positives = 15/19 (78%)
Frame = +3
Query: 136 DDEEDDEDYEDEEEYTDDE 154
DD++DD+ E+E++ DDE
Sbjct: 309 DDDDDDDGEEEEDDDDDDE 365
Score = 25.4 bits (54), Expect = 4.6
Identities = 8/19 (42%), Positives = 16/19 (84%)
Frame = +3
Query: 136 DDEEDDEDYEDEEEYTDDE 154
+D++++ED EDE++ DD+
Sbjct: 261 EDDDEEEDDEDEDDDDDDD 317
Score = 25.0 bits (53), Expect = 5.9
Identities = 20/68 (29%), Positives = 28/68 (40%)
Frame = +3
Query: 86 DDDEEEFNVFRMAAMQEAMVSGIDDFGPDDFPGIDPMDAALISMMMHSHMDDEEDDEDYE 145
DDDEEE DD DD D D ++EEDD+D +
Sbjct: 264 DDDEEE-----------------DDEDEDDDDDDDDDDDG----------EEEEDDDDDD 362
Query: 146 DEEEYTDD 153
+EEE +++
Sbjct: 363 EEEEGSEE 386
>TC17157 similar to UP|CAD27462 (CAD27462) Nucleosome assembly protein
1-like protein 3, partial (81%)
Length = 1298
Score = 28.5 bits (62), Expect = 0.54
Identities = 16/53 (30%), Positives = 26/53 (48%)
Frame = +2
Query: 102 EAMVSGIDDFGPDDFPGIDPMDAALISMMMHSHMDDEEDDEDYEDEEEYTDDE 154
EA S +D DD G + D DD++D+E+ +D+++ DDE
Sbjct: 743 EAEQSDFEDIEEDDEDGDEDEDE-----------DDDDDEEEEDDDDDDEDDE 868
>TC12685 weakly similar to UP|Q7QA92 (Q7QA92) AgCP13961 (Fragment), partial
(9%)
Length = 547
Score = 28.5 bits (62), Expect = 0.54
Identities = 10/16 (62%), Positives = 14/16 (87%)
Frame = +2
Query: 137 DEEDDEDYEDEEEYTD 152
DEEDDE++ED +E+ D
Sbjct: 170 DEEDDEEFEDAKEFDD 217
>AV416122
Length = 414
Score = 28.1 bits (61), Expect = 0.70
Identities = 10/19 (52%), Positives = 16/19 (83%)
Frame = +3
Query: 136 DDEEDDEDYEDEEEYTDDE 154
++E+D+ED E+E+E DDE
Sbjct: 306 EEEDDEEDKEEEDEEEDDE 362
Score = 26.9 bits (58), Expect = 1.6
Identities = 10/19 (52%), Positives = 15/19 (78%)
Frame = +3
Query: 136 DDEEDDEDYEDEEEYTDDE 154
DDEED E+ ++EE+ DD+
Sbjct: 315 DDEEDKEEEDEEEDDEDDD 371
Score = 26.2 bits (56), Expect = 2.7
Identities = 9/19 (47%), Positives = 16/19 (83%)
Frame = +3
Query: 136 DDEEDDEDYEDEEEYTDDE 154
+D+E+D++ EDEEE +D+
Sbjct: 312 EDDEEDKEEEDEEEDDEDD 368
Score = 24.6 bits (52), Expect = 7.8
Identities = 8/19 (42%), Positives = 16/19 (84%)
Frame = +3
Query: 136 DDEEDDEDYEDEEEYTDDE 154
++EEDDE+ ++EE+ +D+
Sbjct: 303 EEEEDDEEDKEEEDEEEDD 359
>BP054131
Length = 500
Score = 28.1 bits (61), Expect = 0.70
Identities = 12/37 (32%), Positives = 20/37 (53%)
Frame = -2
Query: 118 GIDPMDAALISMMMHSHMDDEEDDEDYEDEEEYTDDE 154
G+ + A M D+ E+DE+ +D+ YT+DE
Sbjct: 304 GLKDQEKAKSKSKMDEDEDEVEEDEEMDDDLVYTEDE 194
>BP038231
Length = 513
Score = 28.1 bits (61), Expect = 0.70
Identities = 12/20 (60%), Positives = 14/20 (70%)
Frame = -3
Query: 136 DDEEDDEDYEDEEEYTDDEY 155
DD E D +D+EEY DDEY
Sbjct: 439 DDIEMDGTEDDDEEYFDDEY 380
>TC10643 similar to UP|BAC84847 (BAC84847) Acidic nuclear
phosphoprotein-like protein, partial (5%)
Length = 789
Score = 28.1 bits (61), Expect = 0.70
Identities = 11/28 (39%), Positives = 19/28 (67%)
Frame = +2
Query: 127 ISMMMHSHMDDEEDDEDYEDEEEYTDDE 154
+ MM +++ED+E+ ED EE +DD+
Sbjct: 11 LRMMKLGSGEEDEDEEEEEDSEESSDDD 94
>TC12959 similar to UP|AAS52042 (AAS52042) ADR122Cp, partial (6%)
Length = 517
Score = 27.3 bits (59), Expect = 1.2
Identities = 13/27 (48%), Positives = 18/27 (66%), Gaps = 4/27 (14%)
Frame = +1
Query: 133 SHMDDEEDDEDYEDEE----EYTDDEY 155
S DD++DD+D EDE+ E DDE+
Sbjct: 121 SSSDDDDDDDDSEDEDDDDNEDDDDEW 201
Score = 24.6 bits (52), Expect = 7.8
Identities = 17/48 (35%), Positives = 22/48 (45%), Gaps = 6/48 (12%)
Frame = +1
Query: 21 IMRDSDFFEGMDPFEMMA------LSDSLAGGSGPCLGPFDSDDDDDD 62
++R DF + PF + DSL+ GS D DDDDDD
Sbjct: 25 LLRFWDFNDATKPFTKIEDCPSSRFWDSLSDGSSS-----DDDDDDDD 153
>TC17687 weakly similar to GB|AAH05628.1|13542865|BC005628 acidic nuclear
phosphoprotein 32 family, member B {Mus musculus;},
partial (9%)
Length = 702
Score = 26.9 bits (58), Expect = 1.6
Identities = 10/21 (47%), Positives = 17/21 (80%), Gaps = 2/21 (9%)
Frame = +1
Query: 136 DDEEDDEDYED--EEEYTDDE 154
D++ +DE+Y+D E EY++DE
Sbjct: 322 DEDNEDEEYDDDYESEYSEDE 384
>CB826779
Length = 469
Score = 26.9 bits (58), Expect = 1.6
Identities = 11/27 (40%), Positives = 18/27 (65%)
Frame = +1
Query: 123 DAALISMMMHSHMDDEEDDEDYEDEEE 149
DA ++ + +DE+DDED ED+E+
Sbjct: 190 DAPVVEDVHDEADEDEDDDEDDEDDED 270
Score = 24.6 bits (52), Expect = 7.8
Identities = 10/18 (55%), Positives = 13/18 (71%)
Frame = +1
Query: 136 DDEEDDEDYEDEEEYTDD 153
DD+EDDED ED + T +
Sbjct: 238 DDDEDDEDDEDGAQGTTE 291
>BI420041
Length = 452
Score = 26.9 bits (58), Expect = 1.6
Identities = 18/50 (36%), Positives = 26/50 (52%), Gaps = 9/50 (18%)
Frame = +2
Query: 114 DDFPGIDPMDAALISMMMHSHMDDEEDDE-DY--------EDEEEYTDDE 154
D P ++P +++ MD+EEDDE DY ED +EY DD+
Sbjct: 200 DSEPVLEP------TIVQEVSMDEEEDDEFDYEEPLNDEDEDGDEYYDDD 331
Score = 26.2 bits (56), Expect = 2.7
Identities = 8/14 (57%), Positives = 14/14 (99%)
Frame = +2
Query: 136 DDEEDDEDYEDEEE 149
DDE+++E++EDE+E
Sbjct: 326 DDEQEEEEFEDEDE 367
>TC18955 similar to UP|Q9LU67 (Q9LU67) Phosphatidylserine decarboxylase,
partial (6%)
Length = 523
Score = 26.9 bits (58), Expect = 1.6
Identities = 10/11 (90%), Positives = 10/11 (90%)
Frame = -3
Query: 53 PFDSDDDDDDG 63
PF SDDDDDDG
Sbjct: 53 PFPSDDDDDDG 21
>TC8507 similar to UP|Q8LJS2 (Q8LJS2) Nucleolar histone deacetylase
HD2-P39, partial (40%)
Length = 620
Score = 26.9 bits (58), Expect = 1.6
Identities = 11/18 (61%), Positives = 13/18 (72%)
Frame = +3
Query: 137 DEEDDEDYEDEEEYTDDE 154
DEEDD D ED+E+ DE
Sbjct: 3 DEEDDSDDEDDEDDDSDE 56
Score = 25.8 bits (55), Expect = 3.5
Identities = 8/19 (42%), Positives = 16/19 (84%)
Frame = +3
Query: 136 DDEEDDEDYEDEEEYTDDE 154
D+E+D +D +DE++ +D+E
Sbjct: 3 DEEDDSDDEDDEDDDSDEE 59
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.317 0.138 0.402
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,861,235
Number of Sequences: 28460
Number of extensions: 21208
Number of successful extensions: 767
Number of sequences better than 10.0: 80
Number of HSP's better than 10.0 without gapping: 340
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 591
length of query: 155
length of database: 4,897,600
effective HSP length: 83
effective length of query: 72
effective length of database: 2,535,420
effective search space: 182550240
effective search space used: 182550240
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 51 (24.3 bits)
Medicago: description of AC146566.1