BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146563.9 + phase: 0
(620 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC18807 weakly similar to UP|Q93ZM0 (Q93ZM0) AT3g18370/MYF24_8, ... 233 9e-62
AV425891 72 2e-13
AV779131 39 0.002
CB827472 38 0.004
TC20154 37 0.012
BI418773 36 0.021
BP052407 35 0.047
AW719889 33 0.10
TC14274 weakly similar to GB|AAF79559.1|8778551|AC022464 F22G5.3... 32 0.30
AV766999 30 0.88
AW720487 29 2.0
AW164114 29 2.6
AV777873 28 4.4
TC9277 similar to GB|AAP13413.1|30023760|BT006305 At3g55530 {Ara... 28 5.7
TC11265 weakly similar to UP|Q9FY55 (Q9FY55) CLB1-like protein, ... 27 7.4
>TC18807 weakly similar to UP|Q93ZM0 (Q93ZM0) AT3g18370/MYF24_8, partial
(12%)
Length = 744
Score = 233 bits (593), Expect = 9e-62
Identities = 114/138 (82%), Positives = 123/138 (88%), Gaps = 1/138 (0%)
Frame = +3
Query: 1 MSRKKRVFS-IDSIEEVAVDFFNYVLQEKPKIPFFIPVILIACAVEKWVFSFSTWVPLAL 59
MS KKRV S + SIE+ AVDFFN++LQEKP+I FFIP+ILIA VE+WVF FSTWVP+AL
Sbjct: 330 MSSKKRVISFVSSIEDAAVDFFNHLLQEKPQISFFIPLILIAWVVERWVFPFSTWVPIAL 509
Query: 60 AVWATIQYGRYQRKLLVEDLDKKWKRIILNNSPITPLEHCEWLNKLLTEIWPNYFNPKLS 119
AVW TIQYGRYQRKLLVEDLDKKWKRIILN SPITPLEHCEWLNKLLTEIW NYFN KLS
Sbjct: 510 AVWTTIQYGRYQRKLLVEDLDKKWKRIILNVSPITPLEHCEWLNKLLTEIWSNYFNQKLS 689
Query: 120 SRLSAIVEARLKLRKPRF 137
+RLS IVE RLKLRKPRF
Sbjct: 690 NRLSDIVEKRLKLRKPRF 743
>AV425891
Length = 398
Score = 72.4 bits (176), Expect = 2e-13
Identities = 49/153 (32%), Positives = 75/153 (48%), Gaps = 1/153 (0%)
Frame = +1
Query: 435 EGTNSGELKVSIVVKEWQFSDGTHSLNNLRNNSQQSLNGSSNIQLRTGKKLKITVVEGKD 494
E SGEL++ + +R + Q+ GS N +++ ++EG+D
Sbjct: 4 ERVRSGELRLQVEA--------------VRVDDQEGSTGSGN------GWIELVLIEGRD 123
Query: 495 LAAAKEKTGKFDPYIKLQYGKVMQKTKTSH-TPNPVWNQTIEFDEVGGGEYLKLKVFTEE 553
L AA + G DPY+++ YG + +KTK H T NP WNQT+EF + G L +K
Sbjct: 124 LVAA-DLRGTSDPYVRVHYGNIKKKTKIIHKTLNPQWNQTLEFPDDGSPLVLYVKDH-NA 297
Query: 554 LFGDENIGSAQVNLEGLVDGSVRDVWIPLERVR 586
L +IG V + L + D WIPL+ V+
Sbjct: 298 LLPTSSIGECVVEYQRLPPNQMADKWIPLQGVK 396
Score = 46.2 bits (108), Expect = 2e-05
Identities = 20/25 (80%), Positives = 25/25 (100%)
Frame = +1
Query: 582 LERVRSGEIRLKIEAIKVDDQEGST 606
LERVRSGE+RL++EA++VDDQEGST
Sbjct: 1 LERVRSGELRLQVEAVRVDDQEGST 75
>AV779131
Length = 530
Score = 39.3 bits (90), Expect = 0.002
Identities = 31/112 (27%), Positives = 59/112 (52%), Gaps = 5/112 (4%)
Frame = -2
Query: 489 VVEGKDLAAAKEKTGKFDPYIKLQYGKVMQKTKTS---HTPNPVWNQTIEF-DEVGGGEY 544
V+ ++L A + GK DP++ L K +T + NPVWNQT +F E G +
Sbjct: 529 VISAEELPAV-DFMGKSDPFLVLTLRKADTDNQTRVVYDSLNPVWNQTFDFVVEDGLHDM 353
Query: 545 LKLKVFTEELFGDENIGSAQVNLEGLV-DGSVRDVWIPLERVRSGEIRLKIE 595
L ++V+ + FG + +G + L ++ +G ++ + L+ +SG + L ++
Sbjct: 352 LIVEVWDRDTFGKDYMGRCILTLTRVILEGEYKERF-ELDGAKSGYLNLHLK 200
>CB827472
Length = 544
Score = 38.1 bits (87), Expect = 0.004
Identities = 32/117 (27%), Positives = 57/117 (48%), Gaps = 6/117 (5%)
Frame = +1
Query: 482 GKKLKITVVEGKDLAAAKEKTGKFDPYIKLQYG--KVMQKTKTSHTPNPVWNQTIEFDEV 539
G+ L++TVV L E + DPY+ ++YG K +T T NPV+ + F +
Sbjct: 34 GQPLEVTVVSCSKLKDT-EWISRQDPYVCVEYGNTKFRTRTCTDGGRNPVFQEKFTFPLI 210
Query: 540 GGGEYLKLKVF-TEELFGDENIGSAQVNLEGLVDGSVRDVWIPLERV---RSGEIRL 592
G + + V+ + L D+ IG+ ++ L ++ D PL+ +GE++L
Sbjct: 211 EGLRDINVIVWNSNTLSFDDFIGTGKIQLHKVLSQGYDDSTWPLQSKTGRHAGEVKL 381
>TC20154
Length = 607
Score = 36.6 bits (83), Expect = 0.012
Identities = 17/55 (30%), Positives = 31/55 (55%), Gaps = 1/55 (1%)
Frame = +3
Query: 484 KLKITVVEGKDLAAAKEKTGKFDPYIKLQYGKVMQKTKTSHTP-NPVWNQTIEFD 537
KL + V++ DL K+ G P++++ + + Q+T+T H NP WN+ F+
Sbjct: 183 KLVVEVLDASDLMP-KDGEGSASPFVEVDFDEQQQRTQTKHKDLNPYWNEKFVFN 344
>BI418773
Length = 561
Score = 35.8 bits (81), Expect = 0.021
Identities = 24/91 (26%), Positives = 42/91 (45%), Gaps = 3/91 (3%)
Frame = +3
Query: 485 LKITVVEGKDLAAAKEKTGKFDPYIKLQYGKVMQKTKTSHTP--NPVWNQTIEFDEVGGG 542
L++ +V K L + E DPY+ L Y K+ NP WN+T F
Sbjct: 201 LEVVLVSAKGLDDS-EFLSNIDPYVILAYKAQEHKSTVQEGAGSNPQWNETFLFTVSDSA 377
Query: 543 EYLKLKVFTEELF-GDENIGSAQVNLEGLVD 572
L L++ ++ + D+++G A + L+ L +
Sbjct: 378 SELTLRIMEKDNYNNDDSLGEAIIPLDALFE 470
>BP052407
Length = 469
Score = 34.7 bits (78), Expect = 0.047
Identities = 16/79 (20%), Positives = 37/79 (46%), Gaps = 2/79 (2%)
Frame = -1
Query: 506 DPYIKLQYGK--VMQKTKTSHTPNPVWNQTIEFDEVGGGEYLKLKVFTEELFGDENIGSA 563
DPY+ + + V + + P+WN++ L+ ++ +++FG E +G+
Sbjct: 457 DPYVTVSVPQATVARTRVLKNASKPIWNESFNIPLAHPVVDLEFRIKDDDVFGAETMGTV 278
Query: 564 QVNLEGLVDGSVRDVWIPL 582
++ + ++ G W PL
Sbjct: 277 KIPAKQILSGEEISGWFPL 221
>AW719889
Length = 623
Score = 33.5 bits (75), Expect = 0.10
Identities = 20/83 (24%), Positives = 40/83 (48%), Gaps = 2/83 (2%)
Frame = +2
Query: 97 EHCEWLNKLLTEIWPNYFNPKLSSRLSAIVEARLKLRKPRF-LERVELQEFSLGSCPPSL 155
E +W+NK L ++WP Y + + + ++ ++ +E + SLG+ PP++
Sbjct: 365 ERVDWMNKFLLDMWP-YLEKAICGMIRTTAQPIFAEYIGKYQIKAIEFDKLSLGTLPPTI 541
Query: 156 ALQGMR-WSTIGDQRVMQLGFDW 177
G++ T + VM+ G W
Sbjct: 542 X--GIKVLETNEKELVMEQGIKW 604
>TC14274 weakly similar to GB|AAF79559.1|8778551|AC022464 F22G5.35
{Arabidopsis thaliana;} , partial (16%)
Length = 1208
Score = 32.0 bits (71), Expect = 0.30
Identities = 20/71 (28%), Positives = 32/71 (44%), Gaps = 5/71 (7%)
Frame = +2
Query: 485 LKITVVEGKDLAAAKEKTGKFDPYIKLQYG---KVMQKTKTSHTPNPVWNQ--TIEFDEV 539
L +T+V K L KTG PY+ ++ K+ S +PVWN+ T+
Sbjct: 77 LDLTIVSAKHLKNVNWKTGDLKPYVVFWVDPDRRLATKSDDSGNTSPVWNERFTLPLSHP 256
Query: 540 GGGEYLKLKVF 550
+L L++F
Sbjct: 257 ITDSFLTLEIF 289
>AV766999
Length = 551
Score = 30.4 bits (67), Expect = 0.88
Identities = 20/46 (43%), Positives = 25/46 (53%), Gaps = 3/46 (6%)
Frame = +2
Query: 81 KKW---KRIILNNSPITPLEHCEWLNKLLTEIWPNYFNPKLSSRLS 123
K+W K IL++S P CE + K + I NYFN KLS LS
Sbjct: 38 KRWT*SKGKILDSSNSPPTSLCEEIKKNTS*IG*NYFNDKLSLSLS 175
>AW720487
Length = 575
Score = 29.3 bits (64), Expect = 2.0
Identities = 19/47 (40%), Positives = 26/47 (54%), Gaps = 3/47 (6%)
Frame = +2
Query: 103 NKLLTEIWPNYFNPKLSSRL-SAIVEARLKLRK--PRFLERVELQEF 146
NKL+T WPN N S L ++ E K+RK P+FL+ LQ +
Sbjct: 371 NKLVTAWWPNSVNKVFPSSLQTSSKEESKKMRKMLPQFLKPQALQRY 511
>AW164114
Length = 297
Score = 28.9 bits (63), Expect = 2.6
Identities = 14/30 (46%), Positives = 17/30 (56%), Gaps = 3/30 (10%)
Frame = -1
Query: 37 VILIACAVEKWVFSFSTWVPLA---LAVWA 63
V+ +A WVFSF+ W P A LA WA
Sbjct: 225 VLSLAAWAVCWVFSFAAWAPWAVFSLAAWA 136
>AV777873
Length = 568
Score = 28.1 bits (61), Expect = 4.4
Identities = 15/30 (50%), Positives = 17/30 (56%), Gaps = 3/30 (10%)
Frame = +1
Query: 451 WQFSDGTHSLNNLR---NNSQQSLNGSSNI 477
WQFSDGT + R N S LNGS N+
Sbjct: 250 WQFSDGTKESFSFRYRYNTSCCGLNGSHNV 339
>TC9277 similar to GB|AAP13413.1|30023760|BT006305 At3g55530 {Arabidopsis
thaliana;}, partial (79%)
Length = 1297
Score = 27.7 bits (60), Expect = 5.7
Identities = 13/32 (40%), Positives = 19/32 (58%)
Frame = -3
Query: 557 DENIGSAQVNLEGLVDGSVRDVWIPLERVRSG 588
DE QVNL + G+ +VWIP+E ++ G
Sbjct: 143 DEEEEEQQVNL--IATGTANNVWIPIEEMKVG 54
>TC11265 weakly similar to UP|Q9FY55 (Q9FY55) CLB1-like protein, partial
(16%)
Length = 668
Score = 27.3 bits (59), Expect = 7.4
Identities = 34/127 (26%), Positives = 50/127 (38%), Gaps = 3/127 (2%)
Frame = +2
Query: 292 GIIYVRVISANKLSSSSFKASRRQQSGSTNGSSEDVSDDKDLHTFVEVEIEELTRRTDVR 351
G++YV VISA L +S F + T ++ + +I+ TR +
Sbjct: 74 GVLYVTVISAENLPASHFMGNPDPFVVLTLRKAKS-----------KAKIKNKTRVVNKN 220
Query: 352 LGSTPRWDAPFNMVLHDNTGTLRFNLYECIPNNVKCDYLGSCEIKLRHV--EDDSTIMW- 408
L P WD F + D + + N DY+G C + L V E D T +
Sbjct: 221 L--NPNWDQTFGFDVEDGLHDMVI-VEVWDKNTFGKDYIGRCILSLTKVILEGDYTESFE 391
Query: 409 AVGPDSG 415
VG SG
Sbjct: 392 LVGAKSG 412
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.319 0.137 0.407
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,857,995
Number of Sequences: 28460
Number of extensions: 152999
Number of successful extensions: 733
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 726
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 730
length of query: 620
length of database: 4,897,600
effective HSP length: 96
effective length of query: 524
effective length of database: 2,165,440
effective search space: 1134690560
effective search space used: 1134690560
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 58 (26.9 bits)
Medicago: description of AC146563.9