BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146563.12 - phase: 0
(336 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AW719644 155 1e-38
TC11825 137 3e-33
BP059172 120 3e-28
TC17045 similar to UP|Q99685 (Q99685) Lysophospholipase homolog ... 101 2e-22
TC15035 100 3e-22
TC15260 similar to GB|AAM63479.1|21554372|AY086477 phospholipase... 90 6e-19
TC8405 weakly similar to UP|O04083 (O04083) Lysophospholipase is... 79 1e-15
TC9030 similar to GB|AAM63479.1|21554372|AY086477 phospholipase-... 75 1e-14
AV414308 64 4e-11
TC16763 similar to UP|Q8LEE3 (Q8LEE3) Lysophospholipase-like pro... 57 4e-09
CB829395 52 2e-07
BP085087 41 2e-06
TC9769 29 0.001
BP048197 29 0.001
TC9113 38 0.003
AV406816 29 1.3
TC9154 similar to UP|Q9ZR69 (Q9ZR69) Acetyl-CoA synthetase , pa... 28 1.7
BP041825 28 2.2
BP085348 28 2.2
TC15599 similar to UP|Q84VY8 (Q84VY8) At2g36290, partial (36%) 28 2.2
>AW719644
Length = 604
Score = 155 bits (391), Expect = 1e-38
Identities = 73/120 (60%), Positives = 91/120 (75%)
Frame = +3
Query: 10 GMSEELQNIYLSNMDEAPARRLAREAFKDIQLAIDHCLFQLPADGVKMEEIYEVNSRGLK 69
G++ ELQ I + MDE ARR AREAFKD+QL IDH LF+ +EVNSRG++
Sbjct: 273 GINNELQMILDAKMDEVSARRRAREAFKDVQLGIDHILFKS----------FEVNSRGVE 422
Query: 70 VFSKSWIPEKSPMKGIVYYCHGYADTCTFYFEGVARKLASSGFGVFALDYPGFGLSDGLH 129
+F K+W+P + K V+YCHGY DTCTF+FEG+ARKLASSG+GVFA+DYPGFGLS+GLH
Sbjct: 423 IFCKTWLPGTARPKAAVFYCHGYGDTCTFFFEGIARKLASSGYGVFAMDYPGFGLSEGLH 602
>TC11825
Length = 542
Score = 137 bits (344), Expect = 3e-33
Identities = 64/115 (55%), Positives = 87/115 (75%)
Frame = +2
Query: 213 EEVKENIYRDARKRELAPYNVLFYKDKPRLGTALELLKATQELEQRLEEVSLPLLVMHGE 272
+++ E +R+ KR LA YNV+ Y+D PRL T LELL+ T+E+E ++++VS PLL++HG
Sbjct: 14 QDLAELAFREPSKRNLAVYNVICYEDNPRLKTGLELLRTTKEIESQVQKVSAPLLILHGA 193
Query: 273 ADIITDPSASKALYQKAKVKDKKLCLYKDAFHTLLEGEPDETIFHVLDDIISWLD 327
AD +TDP S+ LY+KA KDK L LY+D +H +LEGEPD+ IF V DDIISWLD
Sbjct: 194 ADKVTDPLVSQFLYEKASSKDKTLKLYEDGYHCILEGEPDDRIFAVHDDIISWLD 358
>BP059172
Length = 450
Score = 120 bits (301), Expect = 3e-28
Identities = 56/77 (72%), Positives = 66/77 (84%)
Frame = -1
Query: 254 ELEQRLEEVSLPLLVMHGEADIITDPSASKALYQKAKVKDKKLCLYKDAFHTLLEGEPDE 313
E+EQRLEEVSLPLL++HGEADI+TDPS S+A Y+KA D+KL LYKDA+H+LLEGEPDE
Sbjct: 450 EIEQRLEEVSLPLLILHGEADIVTDPSVSQAFYEKASSSDQKLKLYKDAYHSLLEGEPDE 271
Query: 314 TIFHVLDDIISWLDDHS 330
I VL DII WLD+HS
Sbjct: 270 VIIPVLSDIILWLDEHS 220
>TC17045 similar to UP|Q99685 (Q99685) Lysophospholipase homolog
(Lysophospholipase-like), partial (6%)
Length = 508
Score = 101 bits (251), Expect = 2e-22
Identities = 46/87 (52%), Positives = 65/87 (73%)
Frame = +1
Query: 241 RLGTALELLKATQELEQRLEEVSLPLLVMHGEADIITDPSASKALYQKAKVKDKKLCLYK 300
RL +ELL AT ++E +L++VS PLL++HG D +TDP S+ LY+KA KDK L +Y+
Sbjct: 1 RLKIGMELLSATHDIESQLQKVSAPLLILHGAEDKVTDPLVSQFLYEKASSKDKTLKIYE 180
Query: 301 DAFHTLLEGEPDETIFHVLDDIISWLD 327
+ +H ++EGEPD+ IF V +DIISWLD
Sbjct: 181 EGYHGIMEGEPDDRIFAVHNDIISWLD 261
>TC15035
Length = 650
Score = 100 bits (249), Expect = 3e-22
Identities = 59/151 (39%), Positives = 85/151 (56%), Gaps = 6/151 (3%)
Frame = +2
Query: 184 EDMIP---HWLVKQILIGVAKV---LPKTKLVPQKEEVKENIYRDARKRELAPYNVLFYK 237
EDM P H + +L GVA +P K+V + RD +K ++ N Y
Sbjct: 5 EDMKPSRVHLFLYGLLFGVADTWAAMPDNKIVGKA-------IRDPQKLKVIASNPRRYT 163
Query: 238 DKPRLGTALELLKATQELEQRLEEVSLPLLVMHGEADIITDPSASKALYQKAKVKDKKLC 297
PR+GT ELL+ TQ ++ +V+ P L HG AD +T PS+SK LY+KA +DK L
Sbjct: 164 GPPRVGTMRELLRVTQYVQDNFSKVTTPFLTAHGTADGVTCPSSSKLLYEKASCEDKTLK 343
Query: 298 LYKDAFHTLLEGEPDETIFHVLDDIISWLDD 328
LY +H+L++GEPDE+ VL D+ W+D+
Sbjct: 344 LYDGMYHSLIQGEPDESANLVLGDMREWIDE 436
>TC15260 similar to GB|AAM63479.1|21554372|AY086477 phospholipase-like
protein {Arabidopsis thaliana;}, partial (38%)
Length = 593
Score = 89.7 bits (221), Expect = 6e-19
Identities = 48/127 (37%), Positives = 74/127 (57%)
Frame = +2
Query: 200 AKVLPKTKLVPQKEEVKENIYRDARKRELAPYNVLFYKDKPRLGTALELLKATQELEQRL 259
A ++P ++VP + + E ++ KR+LA + +PR TA ELL+ +EL+ R
Sbjct: 14 AAIIPTWRVVPTRGSIPEVSFKVEWKRKLAMASPRRTVARPRASTAQELLRICRELQGRY 193
Query: 260 EEVSLPLLVMHGEADIITDPSASKALYQKAKVKDKKLCLYKDAFHTLLEGEPDETIFHVL 319
EEV +P LV HG D++ DP+ + LY +A KDK L +Y +H L+ GEP E + V
Sbjct: 194 EEVEVPFLVAHGGDDVVCDPACVEELYSRAASKDKTLKIYPGMWHQLV-GEPQENVELVF 370
Query: 320 DDIISWL 326
D++ WL
Sbjct: 371 GDMLEWL 391
>TC8405 weakly similar to UP|O04083 (O04083) Lysophospholipase isolog,
25331-24357 (At1g11090), partial (18%)
Length = 552
Score = 78.6 bits (192), Expect = 1e-15
Identities = 43/132 (32%), Positives = 67/132 (50%), Gaps = 14/132 (10%)
Frame = +2
Query: 69 KVFSKSWIPEKSPMKGIVYYCHGYADTCTFYFEGVARKLASSGFGVFALDYPGFGLSDGL 128
K+F++S+IP + +K V+ HGY + F+ + A+ G+ VFA D G G SDGL
Sbjct: 155 KIFTQSFIPLHTQVKATVFMTHGYGSDTGWLFQKICINFATWGYAVFAADLLGHGRSDGL 334
Query: 129 HGYIPSFENLVNDVIEHFSKIK-------------GESMGGAIALNIHFK-QPTAWDGAA 174
GY+ +++ + + F ++ GESMGG + ++FK +P W G
Sbjct: 335 RGYLGDLDSVASASLSFFLHVRRSESYSALPAFLFGESMGGLATILMYFKSEPEVWTGLI 514
Query: 175 LIAPLCKFAEDM 186
APL EDM
Sbjct: 515 FSAPLFVIPEDM 550
>TC9030 similar to GB|AAM63479.1|21554372|AY086477 phospholipase-like
protein {Arabidopsis thaliana;}, partial (53%)
Length = 596
Score = 75.5 bits (184), Expect = 1e-14
Identities = 46/142 (32%), Positives = 71/142 (49%), Gaps = 15/142 (10%)
Frame = +3
Query: 64 NSRGLKVFSKSW--IPEKSPMKGIVYYCHGYADTCTFYFEGVARKLASSGFGVFALDYPG 121
NSRGLK+F++ W +P P+ G + HG+ +++ + A A +GF A+D+ G
Sbjct: 162 NSRGLKLFTQWWTPLPPAKPI-GTLAVVHGFTGESSWFIQLTAVHFAKAGFATCAIDHQG 338
Query: 122 FGLSDGLHGYIPSFENLVNDVIEHFSKIK------------GESMGGAIALNIHFKQPT- 168
G SDGL +IP +V+D I F+ + ES+GGAIAL I ++
Sbjct: 339 HGFSDGLVAHIPDVNPVVDDGISFFNSFRSRFDPSLPSFLYSESLGGAIALLITLRRENP 518
Query: 169 AWDGAALIAPLCKFAEDMIPHW 190
W+G L +C + P W
Sbjct: 519 PWNGLILNGAMCGISAKFKPPW 584
>AV414308
Length = 389
Score = 63.9 bits (154), Expect = 4e-11
Identities = 27/80 (33%), Positives = 46/80 (56%)
Frame = +2
Query: 54 GVKMEEIYEVNSRGLKVFSKSWIPEKSPMKGIVYYCHGYADTCTFYFEGVARKLASSGFG 113
G+ + ++RGL +F++SW+P +P + +++ HGY + ++ F+ LA GF
Sbjct: 149 GITSSKSSFTSTRGLTLFTRSWLPTTTPPRAVIFMVHGYGNDISWTFQATPIFLAQMGFA 328
Query: 114 VFALDYPGFGLSDGLHGYIP 133
FALD G G S GL ++P
Sbjct: 329 CFALDLQGHGHSGGLKAFVP 388
>TC16763 similar to UP|Q8LEE3 (Q8LEE3) Lysophospholipase-like protein,
partial (22%)
Length = 594
Score = 57.0 bits (136), Expect = 4e-09
Identities = 33/85 (38%), Positives = 50/85 (58%), Gaps = 1/85 (1%)
Frame = +2
Query: 244 TALELLKATQELEQRLEEVSLPLLVMHGEADIITDPSASKALYQKAKVKDKKLCLYKDAF 303
T E+L+ + L + + V++P V+HG AD +TDP AS+ LY KA + K + LY
Sbjct: 5 TGHEILRISSYLMRNFKSVTVPFFVLHGTADKVTDPLASQDLYNKAASEFKDIKLYDGFL 184
Query: 304 HTLL-EGEPDETIFHVLDDIISWLD 327
H LL E E +E + DII+W++
Sbjct: 185 HDLLFEPEREE----IALDIINWME 247
>CB829395
Length = 546
Score = 51.6 bits (122), Expect = 2e-07
Identities = 27/62 (43%), Positives = 36/62 (57%)
Frame = +2
Query: 66 RGLKVFSKSWIPEKSPMKGIVYYCHGYADTCTFYFEGVARKLASSGFGVFALDYPGFGLS 125
R +F +SW PE +KGI+ HG + Y + AR+L S FGVFA+D+ G G S
Sbjct: 362 RNNALFCRSWFPESGSLKGIMIIIHGLNEHSGRYAD-FARQLTSCNFGVFAMDWIGHGGS 538
Query: 126 DG 127
DG
Sbjct: 539 DG 544
>BP085087
Length = 205
Score = 40.8 bits (94), Expect(2) = 2e-06
Identities = 21/43 (48%), Positives = 27/43 (61%)
Frame = -1
Query: 285 LYQKAKVKDKKLCLYKDAFHTLLEGEPDETIFHVLDDIISWLD 327
L++KA DK H +LEGEPD+ +F V +DIISWLD
Sbjct: 127 LHEKASSHDK--------VHGILEGEPDDRVFVVHNDIISWLD 23
Score = 26.9 bits (58), Expect(2) = 2e-06
Identities = 10/21 (47%), Positives = 16/21 (75%)
Frame = -3
Query: 260 EEVSLPLLVMHGEADIITDPS 280
++VS PLL+ HG D +T+P+
Sbjct: 185 QKVSTPLLIPHGAEDKVTNPT 123
>TC9769
Length = 690
Score = 29.3 bits (64), Expect(2) = 0.001
Identities = 11/24 (45%), Positives = 18/24 (74%)
Frame = +2
Query: 131 YIPSFENLVNDVIEHFSKIKGESM 154
YIP+++ LV+D IEH+ +KG +
Sbjct: 89 YIPNYDVLVDDDIEHYRIVKGRML 160
Score = 28.9 bits (63), Expect(2) = 0.001
Identities = 15/37 (40%), Positives = 18/37 (48%)
Frame = +1
Query: 94 DTCTFYFEGVARKLASSGFGVFALDYPGFGLSDGLHG 130
D+CTF+FEG FGLS+GLHG
Sbjct: 34 DSCTFFFEG-------------------FGLSEGLHG 87
>BP048197
Length = 529
Score = 29.3 bits (64), Expect(2) = 0.001
Identities = 11/24 (45%), Positives = 18/24 (74%)
Frame = -3
Query: 131 YIPSFENLVNDVIEHFSKIKGESM 154
YIP+++ LV+D IEH+ +KG +
Sbjct: 182 YIPNYDVLVDDDIEHYRIVKGRML 111
Score = 28.9 bits (63), Expect(2) = 0.001
Identities = 15/37 (40%), Positives = 18/37 (48%)
Frame = -2
Query: 94 DTCTFYFEGVARKLASSGFGVFALDYPGFGLSDGLHG 130
D+CTF+FEG FGLS+GLHG
Sbjct: 237 DSCTFFFEG-------------------FGLSEGLHG 184
>TC9113
Length = 564
Score = 37.7 bits (86), Expect = 0.003
Identities = 15/41 (36%), Positives = 27/41 (65%)
Frame = +1
Query: 287 QKAKVKDKKLCLYKDAFHTLLEGEPDETIFHVLDDIISWLD 327
++A+ +DK + +Y+ H+LL GE DE + V D++ WL+
Sbjct: 1 EEARSEDKTIKVYEGMMHSLLFGETDENVETVRSDVLQWLN 123
>AV406816
Length = 417
Score = 28.9 bits (63), Expect = 1.3
Identities = 16/54 (29%), Positives = 28/54 (51%)
Frame = +1
Query: 73 KSWIPEKSPMKGIVYYCHGYADTCTFYFEGVARKLASSGFGVFALDYPGFGLSD 126
K + E + +V + HG+ + + + LA++GF A D+ G+GLSD
Sbjct: 73 KLHVAEIGTGQNVVVFLHGFPEIW-YSWRHQMIALANAGFRAIAPDFRGYGLSD 231
>TC9154 similar to UP|Q9ZR69 (Q9ZR69) Acetyl-CoA synthetase , partial
(18%)
Length = 529
Score = 28.5 bits (62), Expect = 1.7
Identities = 20/89 (22%), Positives = 39/89 (43%)
Frame = +1
Query: 209 VPQKEEVKENIYRDARKRELAPYNVLFYKDKPRLGTALELLKATQELEQRLEEVSLPLLV 268
VP EE+++N+ RK+ DK AL ++ + + + L +++ L
Sbjct: 109 VPYSEELRKNLILTVRKQ----IGAFAAPDKIHWAPALPKTRSGKIMRRILRKIASRQLD 276
Query: 269 MHGEADIITDPSASKALYQKAKVKDKKLC 297
G+ + DP+ L + + + KLC
Sbjct: 277 ELGDTSTLADPNVVNQLIELSDA*NPKLC 363
>BP041825
Length = 554
Score = 28.1 bits (61), Expect = 2.2
Identities = 17/59 (28%), Positives = 31/59 (51%)
Frame = -2
Query: 172 GAALIAPLCKFAEDMIPHWLVKQILIGVAKVLPKTKLVPQKEEVKENIYRDARKRELAP 230
G+ ++ P+C +++P WL LI A L T L+ + +K N++ D +L+P
Sbjct: 511 GSIVMKPMCMSLSNLLPPWL-SSTLISPASAL--TTLL--ESCIKNNLFDDFEDEDLSP 350
>BP085348
Length = 486
Score = 28.1 bits (61), Expect = 2.2
Identities = 14/32 (43%), Positives = 21/32 (64%)
Frame = -2
Query: 155 GGAIALNIHFKQPTAWDGAALIAPLCKFAEDM 186
GGA+ +++F P+A +AP+ KFAEDM
Sbjct: 449 GGAVIPHLNFSIPSA-----ALAPIFKFAEDM 369
>TC15599 similar to UP|Q84VY8 (Q84VY8) At2g36290, partial (36%)
Length = 698
Score = 28.1 bits (61), Expect = 2.2
Identities = 26/79 (32%), Positives = 35/79 (43%), Gaps = 7/79 (8%)
Frame = +2
Query: 111 GFGVFALDYPGFGLSDGLHGYIPS-----FENLVNDVIEHFSK--IKGESMGGAIALNIH 163
G + + D PG+G SD P E+L D +E SK + G SMGG I +
Sbjct: 464 GIYIVSFDRPGYGESDPDPNRTPKSIAFDIEDLA-DKLELGSKFFLTGLSMGGQIVWSCL 640
Query: 164 FKQPTAWDGAALIAPLCKF 182
P GAAL+ P +
Sbjct: 641 KYIPHRLAGAALLTPAVNY 697
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.319 0.138 0.408
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,551,188
Number of Sequences: 28460
Number of extensions: 72573
Number of successful extensions: 327
Number of sequences better than 10.0: 45
Number of HSP's better than 10.0 without gapping: 318
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 318
length of query: 336
length of database: 4,897,600
effective HSP length: 91
effective length of query: 245
effective length of database: 2,307,740
effective search space: 565396300
effective search space used: 565396300
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)
Medicago: description of AC146563.12