BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146563.10 + phase: 0
(231 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV425891 221 8e-59
TC16468 155 4e-39
TC11979 similar to UP|Q9LVH4 (Q9LVH4) GTPase activating protein-... 46 6e-06
AV779131 46 6e-06
TC20154 38 0.001
AV406506 38 0.002
TC18684 homologue to UP|Q94JQ8 (Q94JQ8) AT3g57880/T10K17_90, par... 37 0.002
AV780095 33 0.042
CB827472 32 0.12
TC19120 27 2.3
AV769583 27 3.0
TC15087 similar to UP|Q9M4Y9 (Q9M4Y9) AP2-related transcription ... 27 3.0
AV407344 27 3.0
NP459447 plasma membrane intrinsic protein homolog [Lotus japoni... 26 5.1
BG662228 26 6.7
>AV425891
Length = 398
Score = 221 bits (563), Expect = 8e-59
Identities = 102/117 (87%), Positives = 110/117 (93%)
Frame = +1
Query: 2 IHFDNNDQGSGSGNGWIELVLIEGRDLVAADLRGTSDPYVRVHYGNFKKRTKVIYKTLTP 61
+ D+ + +GSGNGWIELVLIEGRDLVAADLRGTSDPYVRVHYGN KK+TK+I+KTL P
Sbjct: 46 VRVDDQEGSTGSGNGWIELVLIEGRDLVAADLRGTSDPYVRVHYGNIKKKTKIIHKTLNP 225
Query: 62 QWNQTLEFPDDGSPLMLYVKDHNALLPTSSIGECVVEYQRLPPNQMADKWIPLQGVK 118
QWNQTLEFPDDGSPL+LYVKDHNALLPTSSIGECVVEYQRLPPNQMADKWIPLQGVK
Sbjct: 226 QWNQTLEFPDDGSPLVLYVKDHNALLPTSSIGECVVEYQRLPPNQMADKWIPLQGVK 396
>TC16468
Length = 807
Score = 155 bits (393), Expect = 4e-39
Identities = 83/98 (84%), Positives = 90/98 (91%)
Frame = +3
Query: 132 EMQKRQSMDSEPSLSKLHQIPTQIKQMMIKFRSQIEDGNLEGLSTTLSELETLEDTQEGY 191
E+QKR+S+DSEPSL KLHQIP+QIK+MMIKFRS IED NLEGLSTTLSELETLEDTQEGY
Sbjct: 3 EVQKRKSIDSEPSLGKLHQIPSQIKEMMIKFRSLIEDENLEGLSTTLSELETLEDTQEGY 182
Query: 192 VAQLETEQMLLLSKIKELGQEIINSSPSPSLSRRISES 229
+ QLETEQMLLLS+IKELGQEIINSSPS SRR SES
Sbjct: 183 IVQLETEQMLLLSQIKELGQEIINSSPSQ--SRRSSES 290
>TC11979 similar to UP|Q9LVH4 (Q9LVH4) GTPase activating protein-like,
partial (94%)
Length = 711
Score = 45.8 bits (107), Expect = 6e-06
Identities = 29/85 (34%), Positives = 44/85 (51%), Gaps = 2/85 (2%)
Frame = +1
Query: 16 GWIELVLIEGRDLVAADLRGTSDPYVRVHYGNFKKRTKVIYKTLTPQWNQ--TLEFPDDG 73
G + + + G +L D+R +SDPYV V N K +T+VI K + P+WN+ TL D
Sbjct: 223 GLLRVRVKRGVNLAVRDVR-SSDPYVVVKMYNQKLKTRVIKKDVNPEWNEDLTLSVIDPH 399
Query: 74 SPLMLYVKDHNALLPTSSIGECVVE 98
+ L V DH+ +G+ E
Sbjct: 400 HSVWLTVYDHDTFSKDDKMGDAEFE 474
>AV779131
Length = 530
Score = 45.8 bits (107), Expect = 6e-06
Identities = 30/109 (27%), Positives = 53/109 (48%), Gaps = 4/109 (3%)
Frame = -2
Query: 22 LIEGRDLVAADLRGTSDPYV--RVHYGNFKKRTKVIYKTLTPQWNQTLEF-PDDGSPLML 78
+I +L A D G SDP++ + + +T+V+Y +L P WNQT +F +DG ML
Sbjct: 529 VISAEELPAVDFMGKSDPFLVLTLRKADTDNQTRVVYDSLNPVWNQTFDFVVEDGLHDML 350
Query: 79 YVKDHNA-LLPTSSIGECVVEYQRLPPNQMADKWIPLQGVKRGEIHIQI 126
V+ + +G C++ R+ + L G K G +++ +
Sbjct: 349 IVEVWDRDTFGKDYMGRCILTLTRVILEGEYKERFELDGAKSGYLNLHL 203
>TC20154
Length = 607
Score = 38.1 bits (87), Expect = 0.001
Identities = 15/48 (31%), Positives = 28/48 (58%)
Frame = +3
Query: 22 LIEGRDLVAADLRGTSDPYVRVHYGNFKKRTKVIYKTLTPQWNQTLEF 69
+++ DL+ D G++ P+V V + ++RT+ +K L P WN+ F
Sbjct: 198 VLDASDLMPKDGEGSASPFVEVDFDEQQQRTQTKHKDLNPYWNEKFVF 341
>AV406506
Length = 399
Score = 37.7 bits (86), Expect = 0.002
Identities = 20/43 (46%), Positives = 30/43 (69%), Gaps = 1/43 (2%)
Frame = +3
Query: 9 QGSGSGNGW-IELVLIEGRDLVAADLRGTSDPYVRVHYGNFKK 50
+G + +G+ I+L L+ ++LVAA+L GTSDPYV GN K+
Sbjct: 267 KGDSASSGYLIKLELLAAKNLVAANLNGTSDPYVINTCGNEKR 395
>TC18684 homologue to UP|Q94JQ8 (Q94JQ8) AT3g57880/T10K17_90, partial (16%)
Length = 369
Score = 37.4 bits (85), Expect = 0.002
Identities = 26/76 (34%), Positives = 39/76 (51%), Gaps = 5/76 (6%)
Frame = +2
Query: 13 SGNGWIELVLIEGRDLV---AADLRGTSDPYVRVHYGNFKKRTKVIYKTLTPQWNQ--TL 67
S G +EL ++ + L+ D RGT+D Y YG RT+ I + TP+WN+ T
Sbjct: 35 SSIGVLELGILNAQGLMPMKTKDGRGTTDAYCVAKYGQKWVRTRTIIDSFTPRWNEQYTW 214
Query: 68 EFPDDGSPLMLYVKDH 83
E D + + L V D+
Sbjct: 215 EVFDPCTVITLGVFDN 262
>AV780095
Length = 511
Score = 33.1 bits (74), Expect = 0.042
Identities = 23/79 (29%), Positives = 36/79 (45%), Gaps = 6/79 (7%)
Frame = -1
Query: 16 GWIELVLIEGRDLVAADLRGTSDPYVRVHYGNFK---KRTKVIYKTLTPQWNQTLEF--- 69
G + + ++ L+ DL GTSDPY+ + + K+T V K L P WN+ +
Sbjct: 436 GILHVNVVRALKLLKMDLLGTSDPYL*LSMTGERLPAKKTTVQRKNLNPVWNEKFKLIVK 257
Query: 70 PDDGSPLMLYVKDHNALLP 88
L L V D + + P
Sbjct: 256 DPHSQVLQLQVYDWDKVFP 200
>CB827472
Length = 544
Score = 31.6 bits (70), Expect = 0.12
Identities = 31/127 (24%), Positives = 51/127 (39%), Gaps = 6/127 (4%)
Frame = +1
Query: 18 IELVLIEGRDLVAADLRGTSDPYVRVHYGNFKKRTKVIYK-TLTPQWNQTLEFP--DDGS 74
+E+ ++ L + DPYV V YGN K RT+ P + + FP +
Sbjct: 43 LEVTVVSCSKLKDTEWISRQDPYVCVEYGNTKFRTRTCTDGGRNPVFQEKFTFPLIEGLR 222
Query: 75 PLMLYVKDHNALLPTSSIGECVVEYQRLPPNQMADKWIPLQ---GVKRGEIHIQITRKVP 131
+ + V + N L IG ++ ++ D PLQ G GE+ + + +P
Sbjct: 223 DINVIVWNSNTLSFDDFIGTGKIQLHKVLSQGYDDSTWPLQSKTGRHAGEVKLILHYAMP 402
Query: 132 EMQKRQS 138
Q S
Sbjct: 403 NHQLHNS 423
>TC19120
Length = 479
Score = 27.3 bits (59), Expect = 2.3
Identities = 17/40 (42%), Positives = 22/40 (54%), Gaps = 1/40 (2%)
Frame = +1
Query: 52 TKVIYKTLTPQWNQTLEFPDDG-SPLMLYVKDHNALLPTS 90
TK Y +P+ + L FP SPLM+Y KD N L T+
Sbjct: 52 TKAFYPCGSPRHIRALVFPRLSLSPLMIYFKD*NGLRLTN 171
>AV769583
Length = 572
Score = 26.9 bits (58), Expect = 3.0
Identities = 15/52 (28%), Positives = 23/52 (43%)
Frame = +3
Query: 28 LVAADLRGTSDPYVRVHYGNFKKRTKVIYKTLTPQWNQTLEFPDDGSPLMLY 79
+ A + + +P V V +F K +Y LT W + DD +MLY
Sbjct: 264 IFGAPISNSGNPPVYVRSSSFSK----LYPCLTDNWGELPLKEDDSEDMMLY 407
>TC15087 similar to UP|Q9M4Y9 (Q9M4Y9) AP2-related transcription factor
(Ethylene responsive protein), partial (26%)
Length = 440
Score = 26.9 bits (58), Expect = 3.0
Identities = 15/52 (28%), Positives = 23/52 (43%)
Frame = +2
Query: 28 LVAADLRGTSDPYVRVHYGNFKKRTKVIYKTLTPQWNQTLEFPDDGSPLMLY 79
+ A + + +P V V +F K +Y LT W + DD +MLY
Sbjct: 161 IFGAPISNSGNPPVYVRSSSFSK----LYPCLTDNWGELPLKEDDSEDMMLY 304
>AV407344
Length = 415
Score = 26.9 bits (58), Expect = 3.0
Identities = 26/94 (27%), Positives = 43/94 (45%)
Frame = +1
Query: 138 SMDSEPSLSKLHQIPTQIKQMMIKFRSQIEDGNLEGLSTTLSELETLEDTQEGYVAQLET 197
S S S S ++ + T Q+ +I+ L+ LS+T S T + Q
Sbjct: 85 SKGSSLSSSSVYAVATGRSQV------RIDSSLLDRLSSTTSIKHTFSIPDFLTLEQSRA 246
Query: 198 EQMLLLSKIKELGQEIINSSPSPSLSRRISESVN 231
++LL+K+ ++SP P + RISES+N
Sbjct: 247 FLLVLLNKLLHC-----SNSPGPVIPLRISESLN 333
>NP459447 plasma membrane intrinsic protein homolog [Lotus japonicus]
Length = 578
Score = 26.2 bits (56), Expect = 5.1
Identities = 17/51 (33%), Positives = 26/51 (50%), Gaps = 2/51 (3%)
Frame = -1
Query: 102 LPPNQMADKWIPLQGVKRGEIHIQITRK-VPEMQKRQSMDSE-PSLSKLHQ 150
+PP A WIP + G++ IT K V K +++S P+L K H+
Sbjct: 200 IPPKAQAIPWIPTEAHMFGDLFTPITVKTVMYRNKNVAINSAIPALQKDHE 48
>BG662228
Length = 390
Score = 25.8 bits (55), Expect = 6.7
Identities = 8/21 (38%), Positives = 12/21 (57%)
Frame = +3
Query: 50 KRTKVIYKTLTPQWNQTLEFP 70
K+TK + P WN+ E+P
Sbjct: 108 KKTKAVEDNWLPSWNEVFEYP 170
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.314 0.133 0.377
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,602,137
Number of Sequences: 28460
Number of extensions: 44622
Number of successful extensions: 192
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 192
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 192
length of query: 231
length of database: 4,897,600
effective HSP length: 87
effective length of query: 144
effective length of database: 2,421,580
effective search space: 348707520
effective search space used: 348707520
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 54 (25.4 bits)
Medicago: description of AC146563.10