BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146561.3 + phase: 0
(262 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV407450 105 6e-24
AU251535 74 3e-14
TC19772 46 6e-06
AU089216 33 0.050
AV414527 30 0.55
AV766383 29 0.94
BP038942 28 1.2
TC16187 similar to UP|Q93122 (Q93122) AsSLR8.97, partial (19%) 28 2.1
AV772713 28 2.1
BP074951 28 2.1
AI967538 27 2.7
BP055634 27 2.7
TC13314 similar to UP|FL3L_MOUSE (P49772) SL cytokine precursor ... 27 4.7
TC8360 weakly similar to UP|O22940 (O22940) At2g41800 protein (A... 27 4.7
TC18290 similar to UP|Q84XY7 (Q84XY7) Wax synthase isoform 3 , ... 26 6.1
AV777410 26 6.1
TC15133 similar to UP|O49553 (O49553) G10-like protein (AT4G2111... 26 6.1
TC13171 26 6.1
TC13226 similar to UP|HMGB_SOYBN (Q10370) HMG-Y related protein ... 26 8.0
TC9228 weakly similar to UP|Q8YPY5 (Q8YPY5) Glycine cleavage T-p... 26 8.0
>AV407450
Length = 337
Score = 105 bits (263), Expect = 6e-24
Identities = 57/81 (70%), Positives = 66/81 (81%), Gaps = 5/81 (6%)
Frame = +2
Query: 1 MELSSSFSLSPKP-----TNFSFSSSFSPFPIQILIKNTKSRNYPLHVLAVAIDPTKDFP 55
MELSSSF LSPKP +N+SFSSS SPFPIQILIKN +SRN +LAVA+DPTK+
Sbjct: 98 MELSSSFLLSPKPCSSPSSNYSFSSSISPFPIQILIKNPQSRNPSFRILAVAVDPTKELS 277
Query: 56 RNNNPQRLLKELAERKKITNP 76
+ N+PQRLLKELAERKKIT+P
Sbjct: 278 K-NSPQRLLKELAERKKITSP 337
>AU251535
Length = 359
Score = 73.6 bits (179), Expect = 3e-14
Identities = 43/91 (47%), Positives = 58/91 (63%)
Frame = +2
Query: 1 MELSSSFSLSPKPTNFSFSSSFSPFPIQILIKNTKSRNYPLHVLAVAIDPTKDFPRNNNP 60
MELSSSF L KP + SSS+ P++I K L + AVAIDP N+P
Sbjct: 98 MELSSSFLLPHKPLH---SSSYLHNPLRITTKTRHPTTQILRISAVAIDPPPQELPQNSP 268
Query: 61 QRLLKELAERKKITNPKSKSPPRRYILKPPL 91
+RLL+ELAERK+ +PK ++PPRR++L PP+
Sbjct: 269 RRLLQELAERKR-PSPKRRAPPRRFVLTPPV 358
>TC19772
Length = 570
Score = 46.2 bits (108), Expect = 6e-06
Identities = 17/25 (68%), Positives = 25/25 (100%)
Frame = +1
Query: 238 SLQNLIFPYDNMDKIVRRDREGPVK 262
SLQNL+FPYD++DK++R+D+EGP+K
Sbjct: 1 SLQNLVFPYDDIDKMLRKDKEGPIK 75
Score = 37.4 bits (85), Expect = 0.003
Identities = 25/89 (28%), Positives = 44/89 (49%), Gaps = 2/89 (2%)
Frame = +1
Query: 113 LLSSCLPSLRLNNADKLWIDEYLLEAKQALGYSLEPSETLEDDNPAKQFD--TLLYLAFQ 170
L +C+P +W + + +ALGY LE ++ +++ A+ + L L F
Sbjct: 310 LFRACVPPGMHRFRGNIWDFDCKPQVMKALGYPLEMTDRIQEITEARNIELGLGLQLCFM 489
Query: 171 HPSCERTKQKHVRSGHSRLFFLGQYVLEL 199
HPS + K +H R RL ++GQ + +L
Sbjct: 490 HPS--KYKFEHPRFCFERLEYVGQKIQDL 570
>AU089216
Length = 273
Score = 33.1 bits (74), Expect = 0.050
Identities = 20/58 (34%), Positives = 28/58 (47%)
Frame = +1
Query: 156 NPAKQFDTLLYLAFQHPSCERTKQKHVRSGHSRLFFLGQYVLELALAEFFLQRYPREL 213
N + Q +LL A H SC RT+ + + RL FLG L+ + +F YP L
Sbjct: 31 NYSFQDPSLLVXALTHGSCNRTEFPKAFN-YQRLXFLGDAXLDYVITMYFYNEYPVHL 201
>AV414527
Length = 424
Score = 29.6 bits (65), Expect = 0.55
Identities = 20/65 (30%), Positives = 35/65 (53%)
Frame = +1
Query: 29 ILIKNTKSRNYPLHVLAVAIDPTKDFPRNNNPQRLLKELAERKKITNPKSKSPPRRYILK 88
IL + KSR+ LH L V ++P K+ QRL+ L + + N K + PR+ + +
Sbjct: 88 ILKFHQKSRSLLLHCLVVPLNPRKEHQLAIRFQRLV--LVLQTDLRNRKLQFYPRKQLQR 261
Query: 89 PPLDD 93
P+++
Sbjct: 262 KPINN 276
>AV766383
Length = 474
Score = 28.9 bits (63), Expect = 0.94
Identities = 18/57 (31%), Positives = 28/57 (48%), Gaps = 2/57 (3%)
Frame = +2
Query: 36 SRNYPLHVL--AVAIDPTKDFPRNNNPQRLLKELAERKKITNPKSKSPPRRYILKPP 90
SR +P H+L A A P P N+ R + + RK + + +PPR + +PP
Sbjct: 158 SRAHPFHLLPPASATRPETTPPSINSRGRTDRHTSSRKTVYCRRP*APPRHRLQRPP 328
>BP038942
Length = 617
Score = 28.5 bits (62), Expect = 1.2
Identities = 16/46 (34%), Positives = 19/46 (40%)
Frame = +3
Query: 74 TNPKSKSPPRRYILKPPLDDKKLAERFLNSPQLSLKSFPLLSSCLP 119
++P SK PP L PP L+ F P L P SS P
Sbjct: 201 SSPPSKQPPPPPTLNPPSCSFSLSTNFPTGPSFKLAYSPTASSLPP 338
>TC16187 similar to UP|Q93122 (Q93122) AsSLR8.97, partial (19%)
Length = 500
Score = 27.7 bits (60), Expect = 2.1
Identities = 15/30 (50%), Positives = 18/30 (60%)
Frame = +1
Query: 5 SSFSLSPKPTNFSFSSSFSPFPIQILIKNT 34
+SFS +P+P FS S S S IQ L NT
Sbjct: 55 TSFSQNPQPQAFSISQSISICQIQFLPINT 144
>AV772713
Length = 507
Score = 27.7 bits (60), Expect = 2.1
Identities = 18/49 (36%), Positives = 28/49 (56%), Gaps = 2/49 (4%)
Frame = -2
Query: 4 SSSFSLSPKPTNFSFSSSF--SPFPIQILIKNTKSRNYPLHVLAVAIDP 50
SSS S++P+PT S S+SF SP P L+ + + P+ L ++P
Sbjct: 491 SSSASVAPQPTASSTSTSFYTSPSPSAGLVVDQSLPSTPITSLG*VMEP 345
>BP074951
Length = 548
Score = 27.7 bits (60), Expect = 2.1
Identities = 21/58 (36%), Positives = 30/58 (51%)
Frame = -1
Query: 3 LSSSFSLSPKPTNFSFSSSFSPFPIQILIKNTKSRNYPLHVLAVAIDPTKDFPRNNNP 60
LS+ SLS KP++ S SS +P LIK S PL + V + ++ +NN P
Sbjct: 350 LSTEHSLSRKPSHDSSSS----YPTDRLIKPEISVPTPLACIPVQLVFSRLLEKNNRP 189
>AI967538
Length = 432
Score = 27.3 bits (59), Expect = 2.7
Identities = 34/101 (33%), Positives = 43/101 (41%)
Frame = -2
Query: 25 FPIQILIKNTKSRNYPLHVLAVAIDPTKDFPRNNNPQRLLKELAERKKITNPKSKSPPRR 84
FPI ILIK+TKS Y F R+N LL L + KI S
Sbjct: 410 FPITILIKHTKSTLY--------------FHRSNTTTFLL-SLHKLFKIDESISIQ---- 288
Query: 85 YILKPPLDDKKLAERFLNSPQLSLKSFPLLSSCLPSLRLNN 125
+KPP K LA L+SP L L + C P +L++
Sbjct: 287 --IKPP--GKYLAHHELSSPYLELSGW----LCTPVEKLSH 189
>BP055634
Length = 561
Score = 27.3 bits (59), Expect = 2.7
Identities = 12/21 (57%), Positives = 17/21 (80%)
Frame = -3
Query: 151 TLEDDNPAKQFDTLLYLAFQH 171
T+EDD+ +KQ TLL+LA Q+
Sbjct: 325 TMEDDSASKQSKTLLFLANQN 263
>TC13314 similar to UP|FL3L_MOUSE (P49772) SL cytokine precursor
(Fms-related tyrosine kinase 3 ligand) (Flt3 ligand)
(Flt3L), partial (6%)
Length = 544
Score = 26.6 bits (57), Expect = 4.7
Identities = 12/20 (60%), Positives = 15/20 (75%)
Frame = -2
Query: 4 SSSFSLSPKPTNFSFSSSFS 23
SS ++SP T FSFS+SFS
Sbjct: 261 SSHLTMSPSSTLFSFSNSFS 202
>TC8360 weakly similar to UP|O22940 (O22940) At2g41800 protein
(At2g41800/T11A7.10), partial (82%)
Length = 1366
Score = 26.6 bits (57), Expect = 4.7
Identities = 12/22 (54%), Positives = 14/22 (63%)
Frame = +2
Query: 212 ELPGPMRERVYGLIGKENLPKW 233
ELP P + LIGK +LPKW
Sbjct: 179 ELPNPRFIKKTKLIGKYSLPKW 244
>TC18290 similar to UP|Q84XY7 (Q84XY7) Wax synthase isoform 3 , partial
(7%)
Length = 507
Score = 26.2 bits (56), Expect = 6.1
Identities = 20/66 (30%), Positives = 30/66 (45%), Gaps = 1/66 (1%)
Frame = -3
Query: 27 IQILIKNTKSRNYPLHVLAVAIDPTKDFPRNNNPQRLLKELAERKKITNP-KSKSPPRRY 85
+ L K T+S + P +L I PT R PQR + R+ +P + PP +
Sbjct: 226 VHTLHKTTQSSHLPRRLL---IKPTPHKRRQKQPQRGGVNKSHREHPRHPRRHPPPPSNH 56
Query: 86 ILKPPL 91
+L P L
Sbjct: 55 LLYPNL 38
>AV777410
Length = 596
Score = 26.2 bits (56), Expect = 6.1
Identities = 15/64 (23%), Positives = 31/64 (48%), Gaps = 1/64 (1%)
Frame = -2
Query: 100 FLNSPQLSLKSFP-LLSSCLPSLRLNNADKLWIDEYLLEAKQALGYSLEPSETLEDDNPA 158
F+ P +L + P L +C+P +W + + +ALGY LE ++ +++ A
Sbjct: 487 FIQPPPDALFAHPRLFRACVPPGMHRFRGNIWDFDCKPQVMKALGYPLEMTDRIQEITEA 308
Query: 159 KQFD 162
+ +
Sbjct: 307 RNIE 296
>TC15133 similar to UP|O49553 (O49553) G10-like protein (AT4G21110/F7J7_50),
partial (73%)
Length = 848
Score = 26.2 bits (56), Expect = 6.1
Identities = 10/19 (52%), Positives = 13/19 (67%)
Frame = +3
Query: 162 DTLLYLAFQHPSCERTKQK 180
D L +L+F H CER K+K
Sbjct: 636 DKLKFLSFHHSMCEREKKK 692
>TC13171
Length = 598
Score = 26.2 bits (56), Expect = 6.1
Identities = 14/38 (36%), Positives = 22/38 (57%)
Frame = +3
Query: 15 NFSFSSSFSPFPIQILIKNTKSRNYPLHVLAVAIDPTK 52
N SSS++P P + T++R++P L V+ DP K
Sbjct: 432 NPPLSSSYNPTPCTSPVCTTRTRDFP---LPVSCDPRK 536
>TC13226 similar to UP|HMGB_SOYBN (Q10370) HMG-Y related protein B (SB16B
protein) (Fragment), partial (60%)
Length = 474
Score = 25.8 bits (55), Expect = 8.0
Identities = 14/40 (35%), Positives = 19/40 (47%)
Frame = +1
Query: 186 HSRLFFLGQYVLELALAEFFLQRYPRELPGPMRERVYGLI 225
H R+ L Q L L+ PR LPG +R R+ L+
Sbjct: 106 HQRILMLSQSPRWCQLQVAGLEAGPRRLPGRLRRRLRALL 225
>TC9228 weakly similar to UP|Q8YPY5 (Q8YPY5) Glycine cleavage T-protein,
aminomethyltransferase, partial (6%)
Length = 576
Score = 25.8 bits (55), Expect = 8.0
Identities = 13/41 (31%), Positives = 21/41 (50%), Gaps = 3/41 (7%)
Frame = -2
Query: 207 QRYPRELP---GPMRERVYGLIGKENLPKWIKAASLQNLIF 244
Q P+E+P P E+ Y + E + W++ LQ L+F
Sbjct: 353 QSKPKEIPFTNNPTYEKAY*INPTEYIEAWLRGILLQILVF 231
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.318 0.137 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,016,866
Number of Sequences: 28460
Number of extensions: 80661
Number of successful extensions: 580
Number of sequences better than 10.0: 40
Number of HSP's better than 10.0 without gapping: 570
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 576
length of query: 262
length of database: 4,897,600
effective HSP length: 88
effective length of query: 174
effective length of database: 2,393,120
effective search space: 416402880
effective search space used: 416402880
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)
Medicago: description of AC146561.3