BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146560.2 + phase: 0
(220 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BI418455 224 7e-60
TC9089 similar to UP|RHRE_PEA (Q9S8P4) Rhicadhesin receptor prec... 189 3e-49
BP079498 135 7e-33
TC8987 similar to UP|ABPA_PRUPE (Q9ZRA4) Auxin-binding protein A... 125 4e-30
TC16696 similar to UP|Q9AR81 (Q9AR81) Germin-like protein precur... 125 7e-30
TC11836 similar to GB|AAB51577.1|1755176|ATU75199 germin-like pr... 116 3e-27
TC11913 weakly similar to UP|ABPA_PRUPE (Q9ZRA4) Auxin-binding p... 93 3e-20
TC9088 similar to UP|RHRE_PEA (Q9S8P4) Rhicadhesin receptor prec... 68 1e-12
AV423569 32 0.086
TC16753 similar to UP|Q9SIA7 (Q9SIA7) Legumin-like protein, part... 31 0.15
TC19521 similar to UP|O22526 (O22526) Cation-chloride co-transpo... 27 2.8
TC17573 similar to UP|Q93VB2 (Q93VB2) AT3g62770/F26K9_200, parti... 27 2.8
>BI418455
Length = 477
Score = 224 bits (572), Expect = 7e-60
Identities = 114/154 (74%), Positives = 122/154 (79%), Gaps = 3/154 (1%)
Frame = +1
Query: 1 MKSIYLFFTAFLALASCAFAFDPSPLQDFCVAINDTKTGVFVNGKFCKNPKLATPNDFFF 60
M+ +Y T +S A A+DPSPLQDFCV IND K GVFVNGKFCK+P L DFF
Sbjct: 16 MEVVYFLVTILALASSFASAYDPSPLQDFCVGINDPKYGVFVNGKFCKDPALVKAEDFFK 195
Query: 61 SVKEGNTSNPLGSKVTPVTVNDILGLNTLGISLARIDFASRGLNPPHTHPRATEILIVLE 120
V+ GNTSNPLGSKVTPVTV + GLNTLGISLARIDFA +GLNPPHTHPRATEILIVLE
Sbjct: 196 HVEPGNTSNPLGSKVTPVTVEQLFGLNTLGISLARIDFAPKGLNPPHTHPRATEILIVLE 375
Query: 121 GTLYVGFVTSNPE---NRLITKVLNKGDVFVFPI 151
GTLYVGFVTSN NRL TKVLNKGDVFVFPI
Sbjct: 376 GTLYVGFVTSNNANGGNRLFTKVLNKGDVFVFPI 477
>TC9089 similar to UP|RHRE_PEA (Q9S8P4) Rhicadhesin receptor precursor
(Germin-like protein), partial (93%)
Length = 981
Score = 189 bits (480), Expect = 3e-49
Identities = 104/214 (48%), Positives = 134/214 (62%), Gaps = 2/214 (0%)
Frame = +1
Query: 3 SIYLFFTAFLALASCAFAFDPSPLQDFCVAINDTKTGVFVNGKFCKNPKLATPNDFFFSV 62
S+ LF L + AF+ DP LQD CVA D +GV VNG CK +DFF ++
Sbjct: 169 SLLLFALPLALLLTTAFSSDPDYLQDLCVA--DPASGVPVNGFTCKEASKVNASDFFSNI 342
Query: 63 --KEGNTSNPLGSKVTPVTVNDILGLNTLGISLARIDFASRGLNPPHTHPRATEILIVLE 120
K G T+N GS VT V + GLNTLG+SLARID+A GLNPPHTHPRATE++ VLE
Sbjct: 343 LAKPGATNNTFGSLVTGANVQKVPGLNTLGVSLARIDYAPDGLNPPHTHPRATEVVFVLE 522
Query: 121 GTLYVGFVTSNPENRLITKVLNKGDVFVFPIGLIHFQLNVGYGNAVAIAGLSSQNPGVIT 180
G L VGF+T+ N LI+K + KGD+FVFP GL+HFQ N G A ++ +SQ PG +
Sbjct: 523 GELDVGFITTG--NVLISKHIVKGDIFVFPKGLLHFQKNXGKVPAAVLSAFNSQLPGTQS 696
Query: 181 IANAVFGSNPKISSEVLTKAFQVDNNIVDNLQKQ 214
IA +F S P + VLTK FQV ++ ++ +
Sbjct: 697 IATTLFASTPTVPDNVLTKTFQVGTKQIEKIKSR 798
>BP079498
Length = 373
Score = 135 bits (339), Expect = 7e-33
Identities = 67/83 (80%), Positives = 73/83 (87%)
Frame = -2
Query: 138 TKVLNKGDVFVFPIGLIHFQLNVGYGNAVAIAGLSSQNPGVITIANAVFGSNPKISSEVL 197
TKVLNKGDVFVFPI LIHFQLNVG GNAVAIAGLSSQNPGVITIANA+F ++P IS EVL
Sbjct: 372 TKVLNKGDVFVFPISLIHFQLNVGSGNAVAIAGLSSQNPGVITIANALFKASPPISQEVL 193
Query: 198 TKAFQVDNNIVDNLQKQFWVDNN 220
TKA QVD +I+DNL K+ W DNN
Sbjct: 192 TKALQVDKSIIDNLLKKSWYDNN 124
>TC8987 similar to UP|ABPA_PRUPE (Q9ZRA4) Auxin-binding protein ABP19a
precursor, partial (97%)
Length = 985
Score = 125 bits (315), Expect = 4e-30
Identities = 78/201 (38%), Positives = 112/201 (54%), Gaps = 2/201 (0%)
Frame = +1
Query: 14 LASCAFAFDPSPLQDFCVAINDTKTGVFVNGKFCKNPKLATPNDFFFS--VKEGNTSNPL 71
L S + + +QDFCVA D K G CK P T +DF F+ GN +N +
Sbjct: 46 LFSLLLSTSHAAVQDFCVA--DLKGSDGPAGYPCKTPATVTSDDFVFAGLSAPGNITNII 219
Query: 72 GSKVTPVTVNDILGLNTLGISLARIDFASRGLNPPHTHPRATEILIVLEGTLYVGFVTSN 131
G+ VTP V G+N LG+S AR+D G+ P HTHP A E+LIV +G + GF++S
Sbjct: 220 GAAVTPAFVGQFPGVNGLGLSAARLDIGPGGVIPLHTHPGANELLIVTQGHITAGFISS- 396
Query: 132 PENRLITKVLNKGDVFVFPIGLIHFQLNVGYGNAVAIAGLSSQNPGVITIANAVFGSNPK 191
+ + K L KG++ VFP GL+HFQ+ G A+ A SS +PG+ + A+F SN
Sbjct: 397 -ASTVYIKTLKKGELMVFPQGLLHFQVAAGKTKAIGFAIFSSASPGLQILDFALFASN-- 567
Query: 192 ISSEVLTKAFQVDNNIVDNLQ 212
S+ ++TK +D +V L+
Sbjct: 568 FSTPLITKTTFLDPALVKKLK 630
>TC16696 similar to UP|Q9AR81 (Q9AR81) Germin-like protein precursor,
partial (96%)
Length = 834
Score = 125 bits (313), Expect = 7e-30
Identities = 79/189 (41%), Positives = 106/189 (55%), Gaps = 2/189 (1%)
Frame = +1
Query: 3 SIYLFFTAFLALASCAFAFDPSPLQDFCVAINDTKTGVFVNGKFCKNPKLATPNDFFFSV 62
S LF + L+L+ + DFCV G G CK P T +DF +S
Sbjct: 1 STILFILSLLSLSHASVV-------DFCVGDLTFPNGPA--GYACKKPSKVTADDFAYSG 153
Query: 63 K--EGNTSNPLGSKVTPVTVNDILGLNTLGISLARIDFASRGLNPPHTHPRATEILIVLE 120
GNTS + + VTP G+N LGISLAR+D AS G+ P HTHP A+E L+V++
Sbjct: 154 LGIAGNTSYIIKAAVTPAFDAQFPGVNGLGISLARLDLASGGVIPLHTHPGASEALVVVQ 333
Query: 121 GTLYVGFVTSNPENRLITKVLNKGDVFVFPIGLIHFQLNVGYGNAVAIAGLSSQNPGVIT 180
GT+ GFV S +N + K L KGDV VFP GL+HFQ++ G +A+A SS NPG+
Sbjct: 334 GTICAGFVAS--DNTVYLKTLKKGDVMVFPQGLVHFQIHDGGSSALAFVSFSSANPGLQI 507
Query: 181 IANAVFGSN 189
+ A+F S+
Sbjct: 508 LDFALFKSD 534
>TC11836 similar to GB|AAB51577.1|1755176|ATU75199 germin-like protein
{Arabidopsis thaliana;} , partial (56%)
Length = 434
Score = 116 bits (291), Expect = 3e-27
Identities = 62/121 (51%), Positives = 77/121 (63%), Gaps = 2/121 (1%)
Frame = +3
Query: 12 LALASCAFAFDPSPLQDFCVAINDTKTGVFVNGKFCKNPKLATPNDFFFSV--KEGNTSN 69
L L + A DP LQD CVA D + + VNG CK+ DFF ++ K G T+
Sbjct: 78 LTLTTFTAASDPDSLQDICVA--DLSSAIKVNGFVCKSASAVKETDFFSAILAKPGATNT 251
Query: 70 PLGSKVTPVTVNDILGLNTLGISLARIDFASRGLNPPHTHPRATEILIVLEGTLYVGFVT 129
GS VT V I GLNTLG+S++RID+A GLNPPHTHPRATE++ VLEG L VGF+T
Sbjct: 252 TFGSVVTGANVEKIPGLNTLGVSISRIDYAPGGLNPPHTHPRATEVVFVLEGQLDVGFIT 431
Query: 130 S 130
+
Sbjct: 432 T 434
>TC11913 weakly similar to UP|ABPA_PRUPE (Q9ZRA4) Auxin-binding protein
ABP19a precursor, partial (61%)
Length = 530
Score = 93.2 bits (230), Expect = 3e-20
Identities = 46/104 (44%), Positives = 69/104 (66%)
Frame = +1
Query: 86 LNTLGISLARIDFASRGLNPPHTHPRATEILIVLEGTLYVGFVTSNPENRLITKVLNKGD 145
+N LG+S AR+D + G+ P H+HP A+E++I+L+G + VGF+++ +N + K L KGD
Sbjct: 16 INGLGLSAARLDLDAGGVVPIHSHPGASELVIILQGRITVGFIST--DNTVYQKTLMKGD 189
Query: 146 VFVFPIGLIHFQLNVGYGNAVAIAGLSSQNPGVITIANAVFGSN 189
+ V P GL+HFQLN G A A+ SS NP + A+FG+N
Sbjct: 190 IIVIPQGLLHFQLNAGGNRASAVLTFSSTNPVAQLVDVALFGNN 321
>TC9088 similar to UP|RHRE_PEA (Q9S8P4) Rhicadhesin receptor precursor
(Germin-like protein), partial (35%)
Length = 475
Score = 68.2 bits (165), Expect = 1e-12
Identities = 31/70 (44%), Positives = 45/70 (64%)
Frame = +3
Query: 143 KGDVFVFPIGLIHFQLNVGYGNAVAIAGLSSQNPGVITIANAVFGSNPKISSEVLTKAFQ 202
KG++FVFP GL+H+Q N GY A I+ +SQ PG ++I A+F + P + VLTKAFQ
Sbjct: 3 KGEIFVFPKGLVHYQKNNGYWPASVISAFNSQLPGTVSIPLALFAAKPPVPDNVLTKAFQ 182
Query: 203 VDNNIVDNLQ 212
V+ ++
Sbjct: 183 TSTEEVEKIK 212
>AV423569
Length = 351
Score = 32.0 bits (71), Expect = 0.086
Identities = 12/16 (75%), Positives = 14/16 (87%)
Frame = +3
Query: 94 ARIDFASRGLNPPHTH 109
+RID+A GLNPPHTH
Sbjct: 303 SRIDYAPGGLNPPHTH 350
>TC16753 similar to UP|Q9SIA7 (Q9SIA7) Legumin-like protein, partial (42%)
Length = 534
Score = 31.2 bits (69), Expect = 0.15
Identities = 14/48 (29%), Positives = 27/48 (56%)
Frame = +1
Query: 112 ATEILIVLEGTLYVGFVTSNPENRLITKVLNKGDVFVFPIGLIHFQLN 159
++++ VL+GT VG V E +++ + KGD P+G++ + N
Sbjct: 247 SSKVAYVLQGTGVVGIVLPESEEKVVA--IKKGDALALPLGVVTWWYN 384
>TC19521 similar to UP|O22526 (O22526) Cation-chloride co-transporter,
partial (5%)
Length = 556
Score = 26.9 bits (58), Expect = 2.8
Identities = 11/34 (32%), Positives = 18/34 (52%)
Frame = +2
Query: 49 NPKLATPNDFFFSVKEGNTSNPLGSKVTPVTVND 82
NP++ +D + +G +P+G K PV ND
Sbjct: 119 NPEMGADDDVEAAGADGGFRSPIGRKYRPVLAND 220
>TC17573 similar to UP|Q93VB2 (Q93VB2) AT3g62770/F26K9_200, partial (24%)
Length = 556
Score = 26.9 bits (58), Expect = 2.8
Identities = 13/23 (56%), Positives = 14/23 (60%)
Frame = -3
Query: 4 IYLFFTAFLALASCAFAFDPSPL 26
I LF FLAL SC +PSPL
Sbjct: 362 IRLFLFLFLALKSCDIPAEPSPL 294
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.321 0.139 0.411
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,732,185
Number of Sequences: 28460
Number of extensions: 47224
Number of successful extensions: 235
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 223
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 223
length of query: 220
length of database: 4,897,600
effective HSP length: 87
effective length of query: 133
effective length of database: 2,421,580
effective search space: 322070140
effective search space used: 322070140
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 53 (25.0 bits)
Medicago: description of AC146560.2