BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146559.4 - phase: 0 /pseudo
(159 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC14653 weakly similar to UP|Q9FLU7 (Q9FLU7) Phloem-specific lec... 140 8e-35
AV423736 117 1e-27
TC9848 weakly similar to UP|Q8LBR0 (Q8LBR0) Phloem-specific lect... 107 7e-25
TC18680 60 2e-10
TC8152 57 1e-09
TC16811 45 6e-06
AV423946 39 4e-04
TC9742 similar to UP|Q8LBR0 (Q8LBR0) Phloem-specific lectin PP2-... 38 0.001
BP065090 30 0.15
AV423739 29 0.43
TC19774 weakly similar to UP|AAR92268 (AAR92268) At1g47570, part... 27 1.3
TC8205 similar to UP|Q94JW1 (Q94JW1) AT3g15820/MSJ11_22, partial... 26 2.8
TC8408 homologue to UP|CLPA_PEA (P35100) ATP-dependent Clp prote... 26 3.7
TC15114 26 3.7
TC11560 26 3.7
TC16964 similar to UP|Q9SV20 (Q9SV20) Beta-COP-like protein, par... 25 4.8
TC15113 25 4.8
TC8407 homologue to UP|CLPA_PEA (P35100) ATP-dependent Clp prote... 25 6.3
BP066038 25 6.3
AV427270 25 6.3
>TC14653 weakly similar to UP|Q9FLU7 (Q9FLU7) Phloem-specific lectin-like
protein, partial (45%)
Length = 643
Score = 140 bits (354), Expect = 8e-35
Identities = 77/131 (58%), Positives = 95/131 (71%), Gaps = 2/131 (1%)
Frame = +2
Query: 11 MIEVLPEGCIAKILSHTAPVDSCRLSLVCKGFCSAAKSDTVWDRFLPSDLISIISDSPSA 70
+ E LPEGCIA ILS T+P+D RL+LV F SAA SD VWDRFLP D SIIS S S
Sbjct: 101 VFENLPEGCIANILSLTSPLDVGRLALVSSAFRSAADSDAVWDRFLPDDFNSIISHSSST 280
Query: 71 SSLFSTS--PSKKSLYLTLSDHPIVIENGKKSFQLEKQSGRKIYMLSARDISIALGDTPQ 128
++ S+S SKK LYL+LS +P++I +GKKSFQL +G+K YMLSAR +SI GDTP+
Sbjct: 281 AAADSSSSFKSKKDLYLSLSHNPLIIGDGKKSFQL--VNGKKCYMLSARALSIVWGDTPR 454
Query: 129 FWDWPILPESR 139
+W W LPE+R
Sbjct: 455 YWRWISLPEAR 487
>AV423736
Length = 454
Score = 117 bits (292), Expect = 1e-27
Identities = 64/126 (50%), Positives = 83/126 (65%), Gaps = 1/126 (0%)
Frame = +2
Query: 15 LPEGCIAKILSHTAPVDSCRLSLVCKGFCSAAKSDTVWDRFLPSDLISIISDSPSASSL- 73
LPE C+++ILSHT+P D+CR SLV SAA SD VW FLPSD I+S + + S+L
Sbjct: 38 LPEDCVSEILSHTSPPDACRFSLVSSTLRSAADSDMVWRSFLPSDYEDIVSRAVNPSALQ 217
Query: 74 FSTSPSKKSLYLTLSDHPIVIENGKKSFQLEKQSGRKIYMLSARDISIALGDTPQFWDWP 133
FS S K L+ L P++++ G KSF+L+K SG+K Y+LSARD+SI P FW W
Sbjct: 218 FS---SYKQLFHALCS-PLLLDGGNKSFKLDKLSGKKSYILSARDLSITWSSDPMFWSWR 385
Query: 134 ILPESR 139
PESR
Sbjct: 386 SNPESR 403
>TC9848 weakly similar to UP|Q8LBR0 (Q8LBR0) Phloem-specific lectin
PP2-like protein, partial (19%)
Length = 394
Score = 107 bits (268), Expect = 7e-25
Identities = 56/124 (45%), Positives = 82/124 (65%)
Frame = +1
Query: 9 KSMIEVLPEGCIAKILSHTAPVDSCRLSLVCKGFCSAAKSDTVWDRFLPSDLISIISDSP 68
+S+IE LPE C+++ILSHT+P D+CR S++ SAA SD +W FLPSD IIS +
Sbjct: 22 RSIIETLPEECVSEILSHTSPPDACRFSMLSSTLRSAANSDMLWRSFLPSDYSDIISRAL 201
Query: 69 SASSLFSTSPSKKSLYLTLSDHPIVIENGKKSFQLEKQSGRKIYMLSARDISIALGDTPQ 128
+ L S+S S K L+ L +P++++ G F+L+K SG+K Y+LSAR++SI P
Sbjct: 202 NPLFLNSSS-SFKDLFKALC-NPLLLDGGTMIFKLDKSSGKKSYILSARELSITWSSDPL 375
Query: 129 FWDW 132
+W W
Sbjct: 376 YWTW 387
>TC18680
Length = 492
Score = 60.1 bits (144), Expect = 2e-10
Identities = 33/107 (30%), Positives = 58/107 (53%)
Frame = +1
Query: 15 LPEGCIAKILSHTAPVDSCRLSLVCKGFCSAAKSDTVWDRFLPSDLISIISDSPSASSLF 74
+PE C+A++ H P + C L+ + + F AA +D+VW+ LPS+ ++ P
Sbjct: 181 IPENCVARVFLHLTPPEICNLARLNRAFRGAASADSVWETKLPSNYKDLLHALPPER--- 351
Query: 75 STSPSKKSLYLTLSDHPIVIENGKKSFQLEKQSGRKIYMLSARDISI 121
+ SKK ++ LS P ++G K L++ +GR +SA+ +SI
Sbjct: 352 FQNLSKKDIFALLS-RPQPFDDGNKEVWLDRVTGRVCMSISAKALSI 489
>TC8152
Length = 715
Score = 57.4 bits (137), Expect = 1e-09
Identities = 40/129 (31%), Positives = 65/129 (50%), Gaps = 4/129 (3%)
Frame = +1
Query: 15 LPEGCIAKILSHTAPVDSCRLSLVCKGFCSAAKSDTVWDRFLPSDLISII----SDSPSA 70
LPE C+A IL + P C+L+ + + F +A+ +D VW+ LP++ +I+ +D PS
Sbjct: 199 LPESCVALILGYADPPQICQLATLNRAFRAASSADFVWESKLPANYRAIVRKIFTDFPSD 378
Query: 71 SSLFSTSPSKKSLYLTLSDHPIVIENGKKSFQLEKQSGRKIYMLSARDISIALGDTPQFW 130
S SK+ Y L ++ G K L+K +G+ LS + +SI D ++W
Sbjct: 379 S-------SKRDTYAALC-RLNTLDGGTKKAWLDKSTGKICMCLSFKGLSITGIDDRRYW 534
Query: 131 DWPILPESR 139
ESR
Sbjct: 535 KDIHTEESR 561
>TC16811
Length = 509
Score = 45.1 bits (105), Expect = 6e-06
Identities = 28/102 (27%), Positives = 51/102 (49%)
Frame = +2
Query: 8 AKSMIEVLPEGCIAKILSHTAPVDSCRLSLVCKGFCSAAKSDTVWDRFLPSDLISIISDS 67
+K+ ++ +PE CI+ +L P + C+L+ V K F A+ +D +W+ LP +++
Sbjct: 212 SKTGLDDVPENCISSLLMSLDPQEICKLARVNKAFHRASSADFLWESKLPPGYKFLVNKV 391
Query: 68 PSASSLFSTSPSKKSLYLTLSDHPIVIENGKKSFQLEKQSGR 109
L S +KK +Y L P + G K L++ S +
Sbjct: 392 LGEEKL--GSMTKKEIYAKLC-QPNFFDGGAKEIWLDRCSAQ 508
>AV423946
Length = 426
Score = 38.9 bits (89), Expect = 4e-04
Identities = 16/43 (37%), Positives = 26/43 (60%)
Frame = +1
Query: 15 LPEGCIAKILSHTAPVDSCRLSLVCKGFCSAAKSDTVWDRFLP 57
+PE CI+ + + P D C+L+ V + F A+ +D VW+ LP
Sbjct: 241 IPESCISSLFMNLDPPDICKLARVNRAFHRASSADFVWESKLP 369
>TC9742 similar to UP|Q8LBR0 (Q8LBR0) Phloem-specific lectin PP2-like
protein, partial (20%)
Length = 794
Score = 37.7 bits (86), Expect = 0.001
Identities = 15/30 (50%), Positives = 22/30 (73%)
Frame = +2
Query: 101 FQLEKQSGRKIYMLSARDISIALGDTPQFW 130
F+L+K SG+K Y+LSAR++SI P +W
Sbjct: 143 FKLDKSSGKKSYILSARELSITWSSDPLYW 232
>BP065090
Length = 467
Score = 30.4 bits (67), Expect = 0.15
Identities = 16/31 (51%), Positives = 21/31 (67%)
Frame = -3
Query: 57 PSDLISIISDSPSASSLFSTSPSKKSLYLTL 87
P+DL +SPS+SSL+S PS SL+L L
Sbjct: 303 PTDLQPFHQNSPSSSSLWSPPPSLLSLHLLL 211
>AV423739
Length = 465
Score = 28.9 bits (63), Expect = 0.43
Identities = 14/55 (25%), Positives = 29/55 (52%)
Frame = +1
Query: 2 DMKTEEAKSMIEVLPEGCIAKILSHTAPVDSCRLSLVCKGFCSAAKSDTVWDRFL 56
D + + S+++ LP+ + IL P C+++ VC+ + SD +W+R +
Sbjct: 70 DQEDSQDLSVLD-LPDLALECILERLPPSALCQMAGVCRSLRESCVSDHLWERHM 231
>TC19774 weakly similar to UP|AAR92268 (AAR92268) At1g47570, partial (19%)
Length = 642
Score = 27.3 bits (59), Expect = 1.3
Identities = 17/49 (34%), Positives = 24/49 (48%), Gaps = 4/49 (8%)
Frame = +2
Query: 44 SAAKSDTVWDRFLPSDL----ISIISDSPSASSLFSTSPSKKSLYLTLS 88
SA SD +W + +PSD I I SD S S +PS K + ++
Sbjct: 152 SAIPSDQIWAKLVPSDSRYSDIVIWSDVTGICSEISATPSVKRCWCKIA 298
>TC8205 similar to UP|Q94JW1 (Q94JW1) AT3g15820/MSJ11_22, partial (82%)
Length = 1379
Score = 26.2 bits (56), Expect = 2.8
Identities = 13/28 (46%), Positives = 18/28 (63%)
Frame = -1
Query: 57 PSDLISIISDSPSASSLFSTSPSKKSLY 84
PSD + II S SS+FST S +S++
Sbjct: 1127 PSDTLVIIPILRSLSSMFSTCSSNQSMF 1044
>TC8408 homologue to UP|CLPA_PEA (P35100) ATP-dependent Clp protease
ATP-binding subunit clpA homolog, chloroplast precursor
, partial (9%)
Length = 498
Score = 25.8 bits (55), Expect = 3.7
Identities = 15/30 (50%), Positives = 20/30 (66%), Gaps = 3/30 (10%)
Frame = -3
Query: 56 LPSDLISIISDSP---SASSLFSTSPSKKS 82
LPS+ SII++SP S +S+FS S KS
Sbjct: 190 LPSESASIITESPSLISPASIFSAMLSSKS 101
>TC15114
Length = 464
Score = 25.8 bits (55), Expect = 3.7
Identities = 11/21 (52%), Positives = 15/21 (71%)
Frame = -3
Query: 70 ASSLFSTSPSKKSLYLTLSDH 90
AS+LF T +KKS +L + DH
Sbjct: 156 ASTLFETCRTKKSFHLQIFDH 94
>TC11560
Length = 538
Score = 25.8 bits (55), Expect = 3.7
Identities = 10/25 (40%), Positives = 17/25 (68%)
Frame = -2
Query: 91 PIVIENGKKSFQLEKQSGRKIYMLS 115
P + +NG +SF L Q GR++++ S
Sbjct: 78 PRIWQNGSRSFSLLHQLGRRVWLSS 4
>TC16964 similar to UP|Q9SV20 (Q9SV20) Beta-COP-like protein, partial (15%)
Length = 593
Score = 25.4 bits (54), Expect = 4.8
Identities = 14/26 (53%), Positives = 16/26 (60%)
Frame = +2
Query: 58 SDLISIISDSPSASSLFSTSPSKKSL 83
S L S + SPSASS ST+P SL
Sbjct: 485 STLTSTSAVSPSASSAVSTNPRSSSL 562
>TC15113
Length = 530
Score = 25.4 bits (54), Expect = 4.8
Identities = 11/24 (45%), Positives = 15/24 (61%)
Frame = -2
Query: 67 SPSASSLFSTSPSKKSLYLTLSDH 90
S S+LF T +KKS +L + DH
Sbjct: 340 SRKGSTLFETGRTKKSFHLQIFDH 269
>TC8407 homologue to UP|CLPA_PEA (P35100) ATP-dependent Clp protease
ATP-binding subunit clpA homolog, chloroplast precursor
, partial (8%)
Length = 603
Score = 25.0 bits (53), Expect = 6.3
Identities = 15/35 (42%), Positives = 21/35 (59%), Gaps = 3/35 (8%)
Frame = -1
Query: 56 LPSDLISIISDSP---SASSLFSTSPSKKSLYLTL 87
LPS+ S I++SP S +S+FS S KS + L
Sbjct: 192 LPSESASTITESPSLISLASIFSAMLSSKSRIIAL 88
>BP066038
Length = 445
Score = 25.0 bits (53), Expect = 6.3
Identities = 16/31 (51%), Positives = 17/31 (54%), Gaps = 3/31 (9%)
Frame = +1
Query: 65 SDSPSASSLFSTSPS---KKSLYLTLSDHPI 92
S SP+ S FSTSPS S Y T S PI
Sbjct: 13 SSSPTLCSSFSTSPSPPPSPSTYHTPSSPPI 105
>AV427270
Length = 267
Score = 25.0 bits (53), Expect = 6.3
Identities = 11/28 (39%), Positives = 14/28 (49%)
Frame = +3
Query: 24 LSHTAPVDSCRLSLVCKGFCSAAKSDTV 51
L HT+P+ CR S SA+ TV
Sbjct: 99 LGHTSPITRCRFSTSGNNIASASLDGTV 182
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.320 0.133 0.400
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,945,873
Number of Sequences: 28460
Number of extensions: 40771
Number of successful extensions: 302
Number of sequences better than 10.0: 44
Number of HSP's better than 10.0 without gapping: 296
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 297
length of query: 159
length of database: 4,897,600
effective HSP length: 83
effective length of query: 76
effective length of database: 2,535,420
effective search space: 192691920
effective search space used: 192691920
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 51 (24.3 bits)
Medicago: description of AC146559.4