BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146559.3 + phase: 0
(130 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC14653 weakly similar to UP|Q9FLU7 (Q9FLU7) Phloem-specific lec... 157 4e-40
AV423736 117 8e-28
TC9848 weakly similar to UP|Q8LBR0 (Q8LBR0) Phloem-specific lect... 107 9e-25
TC8152 75 4e-15
TC18680 67 1e-12
TC16811 47 1e-06
TC9742 similar to UP|Q8LBR0 (Q8LBR0) Phloem-specific lectin PP2-... 46 2e-06
AV423946 38 6e-04
AV770887 27 1.1
BP033968 27 1.5
BP058319 27 1.5
TC10529 25 3.3
TC10847 similar to UP|Q8RX88 (Q8RX88) AT4g01320/F2N1_21, partial... 25 3.3
BP042733 25 4.3
TC9596 similar to UP|AAR24722 (AAR24722) At5g49400, partial (66%) 25 5.6
BP043724 25 5.6
TC20065 similar to UP|AAR20725 (AAR20725) At5g18690, partial (21%) 25 5.6
BP042082 24 7.3
BP075275 24 7.3
TC17534 similar to AAR99502 (AAR99502) 3,5-epimerase/4-reductase... 24 7.3
>TC14653 weakly similar to UP|Q9FLU7 (Q9FLU7) Phloem-specific lectin-like
protein, partial (45%)
Length = 643
Score = 157 bits (398), Expect = 4e-40
Identities = 79/135 (58%), Positives = 101/135 (74%), Gaps = 6/135 (4%)
Frame = +2
Query: 1 MTEIQDLPEGCIAAIVSRTTPLDAGRLSVFSKTFRSASDSDAVWNQFLPSDISSIISHSP 60
M ++LPEGCIA I+S T+PLD GRL++ S FRSA+DSDAVW++FLP D +SIISHS
Sbjct: 95 MVVFENLPEGCIANILSLTSPLDVGRLALVSSAFRSAADSDAVWDRFLPDDFNSIISHSS 274
Query: 61 SL------TNIPTKKDLYLALSDRPVIIDHDKKSIQLERKSGKKCYMLSARSLAIVWGDD 114
S ++ +KKDLYL+LS P+II KKS QL +GKKCYMLSAR+L+IVWGD
Sbjct: 275 STAAADSSSSFKSKKDLYLSLSHNPLIIGDGKKSFQL--VNGKKCYMLSARALSIVWGDT 448
Query: 115 RRYWNWISMPDSRLA 129
RYW WIS+P++R +
Sbjct: 449 PRYWRWISLPEARFS 493
>AV423736
Length = 454
Score = 117 bits (292), Expect = 8e-28
Identities = 59/123 (47%), Positives = 80/123 (64%), Gaps = 2/123 (1%)
Frame = +2
Query: 7 LPEGCIAAIVSRTTPLDAGRLSVFSKTFRSASDSDAVWNQFLPSDISSIISH--SPSLTN 64
LPE C++ I+S T+P DA R S+ S T RSA+DSD VW FLPSD I+S +PS
Sbjct: 38 LPEDCVSEILSHTSPPDACRFSLVSSTLRSAADSDMVWRSFLPSDYEDIVSRAVNPSALQ 217
Query: 65 IPTKKDLYLALSDRPVIIDHDKKSIQLERKSGKKCYMLSARSLAIVWGDDRRYWNWISMP 124
+ K L+ AL P+++D KS +L++ SGKK Y+LSAR L+I W D +W+W S P
Sbjct: 218 FSSYKQLFHALCS-PLLLDGGNKSFKLDKLSGKKSYILSARDLSITWSSDPMFWSWRSNP 394
Query: 125 DSR 127
+SR
Sbjct: 395 ESR 403
>TC9848 weakly similar to UP|Q8LBR0 (Q8LBR0) Phloem-specific lectin
PP2-like protein, partial (19%)
Length = 394
Score = 107 bits (266), Expect = 9e-25
Identities = 55/120 (45%), Positives = 78/120 (64%), Gaps = 3/120 (2%)
Frame = +1
Query: 4 IQDLPEGCIAAIVSRTTPLDAGRLSVFSKTFRSASDSDAVWNQFLPSDISSIISHSPS-- 61
I+ LPE C++ I+S T+P DA R S+ S T RSA++SD +W FLPSD S IIS + +
Sbjct: 31 IETLPEECVSEILSHTSPPDACRFSMLSSTLRSAANSDMLWRSFLPSDYSDIISRALNPL 210
Query: 62 -LTNIPTKKDLYLALSDRPVIIDHDKKSIQLERKSGKKCYMLSARSLAIVWGDDRRYWNW 120
L + + KDL+ AL + P+++D +L++ SGKK Y+LSAR L+I W D YW W
Sbjct: 211 FLNSSSSFKDLFKALCN-PLLLDGGTMIFKLDKSSGKKSYILSARELSITWSSDPLYWTW 387
>TC8152
Length = 715
Score = 75.1 bits (183), Expect = 4e-15
Identities = 45/125 (36%), Positives = 68/125 (54%), Gaps = 3/125 (2%)
Frame = +1
Query: 6 DLPEGCIAAIVSRTTPLDAGRLSVFSKTFRSASDSDAVWNQFLPSDISSIISHSPSLTNI 65
+LPE C+A I+ P +L+ ++ FR+AS +D VW LP++ +I+ T+
Sbjct: 196 ELPESCVALILGYADPPQICQLATLNRAFRAASSADFVWESKLPANYRAIV--RKIFTDF 369
Query: 66 P---TKKDLYLALSDRPVIIDHDKKSIQLERKSGKKCYMLSARSLAIVWGDDRRYWNWIS 122
P +K+D Y AL R +D K L++ +GK C LS + L+I DDRRYW I
Sbjct: 370 PSDSSKRDTYAALC-RLNTLDGGTKKAWLDKSTGKICMCLSFKGLSITGIDDRRYWKDIH 546
Query: 123 MPDSR 127
+SR
Sbjct: 547 TEESR 561
>TC18680
Length = 492
Score = 66.6 bits (161), Expect = 1e-12
Identities = 36/106 (33%), Positives = 61/106 (56%), Gaps = 2/106 (1%)
Frame = +1
Query: 6 DLPEGCIAAIVSRTTPLDAGRLSVFSKTFRSASDSDAVWNQFLPSDISSIISHSP--SLT 63
D+PE C+A + TP + L+ ++ FR A+ +D+VW LPS+ ++ P
Sbjct: 178 DIPENCVARVFLHLTPPEICNLARLNRAFRGAASADSVWETKLPSNYKDLLHALPPERFQ 357
Query: 64 NIPTKKDLYLALSDRPVIIDHDKKSIQLERKSGKKCYMLSARSLAI 109
N+ +KKD++ AL RP D K + L+R +G+ C +SA++L+I
Sbjct: 358 NL-SKKDIF-ALLSRPQPFDDGNKEVWLDRVTGRVCMSISAKALSI 489
>TC16811
Length = 509
Score = 46.6 bits (109), Expect = 1e-06
Identities = 28/98 (28%), Positives = 48/98 (48%), Gaps = 2/98 (2%)
Frame = +2
Query: 2 TEIQDLPEGCIAAIVSRTTPLDAGRLSVFSKTFRSASDSDAVWNQFLPSDISSIISH--S 59
T + D+PE CI++++ P + +L+ +K F AS +D +W LP +++
Sbjct: 218 TGLDDVPENCISSLLMSLDPQEICKLARVNKAFHRASSADFLWESKLPPGYKFLVNKVLG 397
Query: 60 PSLTNIPTKKDLYLALSDRPVIIDHDKKSIQLERKSGK 97
TKK++Y L +P D K I L+R S +
Sbjct: 398 EEKLGSMTKKEIYAKLC-QPNFFDGGAKEIWLDRCSAQ 508
>TC9742 similar to UP|Q8LBR0 (Q8LBR0) Phloem-specific lectin PP2-like
protein, partial (20%)
Length = 794
Score = 45.8 bits (107), Expect = 2e-06
Identities = 26/72 (36%), Positives = 39/72 (54%)
Frame = +2
Query: 47 FLPSDISSIISHSPSLTNIPTKKDLYLALSDRPVIIDHDKKSIQLERKSGKKCYMLSARS 106
F PS IS +IS + + + I LY+ ++ + +L++ SGKK Y+LSAR
Sbjct: 35 FFPSFISCLISKNRTFSLINLLFMLYI------YVVP*HMQIFKLDKSSGKKSYILSARE 196
Query: 107 LAIVWGDDRRYW 118
L+I W D YW
Sbjct: 197 LSITWSSDPLYW 232
>AV423946
Length = 426
Score = 37.7 bits (86), Expect = 6e-04
Identities = 16/44 (36%), Positives = 25/44 (56%)
Frame = +1
Query: 6 DLPEGCIAAIVSRTTPLDAGRLSVFSKTFRSASDSDAVWNQFLP 49
D+PE CI+++ P D +L+ ++ F AS +D VW LP
Sbjct: 238 DIPESCISSLFMNLDPPDICKLARVNRAFHRASSADFVWESKLP 369
>AV770887
Length = 510
Score = 26.9 bits (58), Expect = 1.1
Identities = 12/31 (38%), Positives = 21/31 (67%), Gaps = 2/31 (6%)
Frame = -2
Query: 89 IQLERKSGKKCYMLS--ARSLAIVWGDDRRY 117
+ L R S +C +S +RSL+++W ++RRY
Sbjct: 383 LSLSRHSSGECPYMSHLSRSLSLLWCEERRY 291
>BP033968
Length = 437
Score = 26.6 bits (57), Expect = 1.5
Identities = 19/47 (40%), Positives = 25/47 (52%), Gaps = 2/47 (4%)
Frame = -1
Query: 53 SSIISHSPSLTNIPTKKDLYLALS--DRPVIIDHDKKSIQLERKSGK 97
SS SHSP+ T P K+D Y ALS +P ++D+ S K K
Sbjct: 356 SSSYSHSPNNTKFPIKRD-YDALSADSKPAYGNNDQVSANPAMKKEK 219
>BP058319
Length = 518
Score = 26.6 bits (57), Expect = 1.5
Identities = 12/34 (35%), Positives = 21/34 (61%), Gaps = 3/34 (8%)
Frame = -2
Query: 40 SDAVWNQFLPSDISSIISHS---PSLTNIPTKKD 70
S+ WN FLP ++ S+ S S P ++N+ +K +
Sbjct: 334 SNRGWNSFLPPELLSLSSQSLGQPEVSNLDSKDE 233
>TC10529
Length = 533
Score = 25.4 bits (54), Expect = 3.3
Identities = 12/30 (40%), Positives = 17/30 (56%), Gaps = 1/30 (3%)
Frame = +3
Query: 6 DLPEGCIAAIVSRTTPLD-AGRLSVFSKTF 34
D+ GC + RT PLD +++FS TF
Sbjct: 318 DVETGCCNIVKRRTNPLDLTSTMNMFSPTF 407
>TC10847 similar to UP|Q8RX88 (Q8RX88) AT4g01320/F2N1_21, partial (24%)
Length = 720
Score = 25.4 bits (54), Expect = 3.3
Identities = 11/23 (47%), Positives = 15/23 (64%)
Frame = -2
Query: 69 KDLYLALSDRPVIIDHDKKSIQL 91
KDLY L+D P ++ D +IQL
Sbjct: 230 KDLYS*LTDSPPVVSQDLTAIQL 162
>BP042733
Length = 543
Score = 25.0 bits (53), Expect = 4.3
Identities = 12/39 (30%), Positives = 19/39 (47%)
Frame = -2
Query: 92 ERKSGKKCYMLSARSLAIVWGDDRRYWNWISMPDSRLAL 130
E K +CY+ A + RR+WN S+P++ L
Sbjct: 383 EWKCYARCYLS*ANRCRRICSGSRRFWNKDSVPNAPCVL 267
>TC9596 similar to UP|AAR24722 (AAR24722) At5g49400, partial (66%)
Length = 1196
Score = 24.6 bits (52), Expect = 5.6
Identities = 14/76 (18%), Positives = 33/76 (43%)
Frame = -3
Query: 36 SASDSDAVWNQFLPSDISSIISHSPSLTNIPTKKDLYLALSDRPVIIDHDKKSIQLERKS 95
S+S+SD + ++ LP + S ++ D P+ + + S+ L
Sbjct: 771 SSSESDELESELLPEE*SDPVT------------------DDEPLSVSKTESSLSLPESE 646
Query: 96 GKKCYMLSARSLAIVW 111
++C++L+ L + +
Sbjct: 645 SERCFLLAFLELDLAF 598
>BP043724
Length = 557
Score = 24.6 bits (52), Expect = 5.6
Identities = 9/15 (60%), Positives = 9/15 (60%)
Frame = -3
Query: 112 GDDRRYWNWISMPDS 126
G DRR WNWI S
Sbjct: 339 GGDRRSWNWIGASTS 295
>TC20065 similar to UP|AAR20725 (AAR20725) At5g18690, partial (21%)
Length = 592
Score = 24.6 bits (52), Expect = 5.6
Identities = 12/33 (36%), Positives = 17/33 (51%)
Frame = +3
Query: 47 FLPSDISSIISHSPSLTNIPTKKDLYLALSDRP 79
F+PS + S S + S +PT +LSD P
Sbjct: 111 FMPSPLLSSYSEAESTPRVPTLSSSPASLSDPP 209
>BP042082
Length = 521
Score = 24.3 bits (51), Expect = 7.3
Identities = 12/40 (30%), Positives = 21/40 (52%)
Frame = +3
Query: 74 ALSDRPVIIDHDKKSIQLERKSGKKCYMLSARSLAIVWGD 113
A++ ++H L+RKS L+ +SL +V+GD
Sbjct: 42 AMNPSEQYVEHGISQQNLKRKSCATVLTLAYQSLGVVYGD 161
>BP075275
Length = 139
Score = 24.3 bits (51), Expect = 7.3
Identities = 11/18 (61%), Positives = 12/18 (66%)
Frame = +2
Query: 39 DSDAVWNQFLPSDISSII 56
DSDA WN+ L S SS I
Sbjct: 53 DSDACWNRSLDSFFSSFI 106
>TC17534 similar to AAR99502 (AAR99502) 3,5-epimerase/4-reductase, partial
(28%)
Length = 520
Score = 24.3 bits (51), Expect = 7.3
Identities = 12/35 (34%), Positives = 19/35 (54%), Gaps = 5/35 (14%)
Frame = +1
Query: 101 MLSARSLAIVWGDDRRYWN-----WISMPDSRLAL 130
++S SLA+VW R+W WI + R++L
Sbjct: 13 LVSGISLALVWSLTMRFWKCIGTMWIRILHIRISL 117
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.319 0.132 0.399
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,287,731
Number of Sequences: 28460
Number of extensions: 26814
Number of successful extensions: 209
Number of sequences better than 10.0: 44
Number of HSP's better than 10.0 without gapping: 204
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 204
length of query: 130
length of database: 4,897,600
effective HSP length: 80
effective length of query: 50
effective length of database: 2,620,800
effective search space: 131040000
effective search space used: 131040000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)
Medicago: description of AC146559.3