BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146557.5 - phase: 0
(146 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC8595 similar to PIR|T09390|T09390 21K protein precursor - alfa... 35 0.005
BG662072 29 0.37
TC12859 weakly similar to UP|CAE55354 (CAE55354) PE-PGRS FAMILY ... 27 1.4
TC11418 similar to GB|AAP12872.1|30017277|BT006223 At3g57480 {Ar... 27 1.4
TC10474 27 1.8
TC10297 similar to UP|G6PC_SOLTU (Q43839) Glucose-6-phosphate 1-... 26 2.4
AV769441 26 3.1
TC19076 25 4.1
AV774634 25 5.3
TC15031 weakly similar to UP|Q69107 (Q69107) LAT protein, partia... 25 5.3
CB826725 25 5.3
TC16349 homologue to UP|PRS6_SOLTU (P54778) 26S protease regulat... 25 5.3
CN825172 25 5.3
TC18115 weakly similar to UP|Q93XD4 (Q93XD4) Thaumatin-like prot... 25 7.0
TC12845 similar to UP|ILV1_BRANA (P27818) Acetolactate synthase ... 25 7.0
TC14229 similar to UP|P93166 (P93166) SCOF-1, partial (38%) 25 7.0
BP055432 24 9.1
TC20072 similar to UP|Q8V7C1 (Q8V7C1) ORF1 (Fragment), partial (... 24 9.1
TC14550 similar to GB|AAL05900.1|15777879|AY055100 AT3g15640/MSJ... 24 9.1
TC15131 similar to UP|Q9FX71 (Q9FX71) T6J4.1 protein, partial (40%) 24 9.1
>TC8595 similar to PIR|T09390|T09390 21K protein precursor - alfalfa
{Medicago sativa;}, partial (93%)
Length = 811
Score = 35.0 bits (79), Expect = 0.005
Identities = 32/114 (28%), Positives = 49/114 (42%)
Frame = +2
Query: 1 MSGTRTPPATAAPDQIMQQRQLPFSSMKPPFLAAADYHRFAPDHRRNQDLETEAIVVKTP 60
+S +TPP ++ P QL S+ PP ++ H + L + + +
Sbjct: 86 ISPPQTPPTSSNPPAAPPNTQLSASNPSPPTPPPSN-----KTHTSSSKLHSPSPSTEPS 250
Query: 61 QLKRKSEAADFEADSGDRMTPGSTAAANSSVQTPVSGKAGKGGKSSRMTKCNRS 114
K S+ A DS R TP ST A SV T ++G A + R ++C RS
Sbjct: 251 PPKPSSQDAKTSGDSNPRSTPPSTTALRRSV-TALTGSAVR----*RSSRCVRS 397
>BG662072
Length = 411
Score = 28.9 bits (63), Expect = 0.37
Identities = 12/21 (57%), Positives = 13/21 (61%)
Frame = -2
Query: 22 LPFSSMKPPFLAAADYHRFAP 42
LPFSS PP A A H+F P
Sbjct: 257 LPFSSCSPPLFAVAGDHQFKP 195
>TC12859 weakly similar to UP|CAE55354 (CAE55354) PE-PGRS FAMILY PROTEIN,
partial (5%)
Length = 528
Score = 26.9 bits (58), Expect = 1.4
Identities = 17/47 (36%), Positives = 18/47 (38%)
Frame = -2
Query: 1 MSGTRTPPATAAPDQIMQQRQLPFSSMKPPFLAAADYHRFAPDHRRN 47
M G+ PP Q PFSS PP A R AP R N
Sbjct: 218 MPGSDPPPPFRPTPPAPPQASSPFSSFGPPVRPGAPSFRPAPPGRFN 78
>TC11418 similar to GB|AAP12872.1|30017277|BT006223 At3g57480 {Arabidopsis
thaliana;}, partial (81%)
Length = 994
Score = 26.9 bits (58), Expect = 1.4
Identities = 23/90 (25%), Positives = 37/90 (40%), Gaps = 2/90 (2%)
Frame = +1
Query: 38 HRFAPDHR--RNQDLETEAIVVKTPQLKRKSEAADFEADSGDRMTPGSTAAANSSVQTPV 95
HRF PDH+ + LET + RK E + S + S++ N++
Sbjct: 466 HRFGPDHKCPGPKKLETSFPFLSLLNKNRKEERKP-NSSSTPSSSKWSSSFLNAASNIRA 642
Query: 96 SGKAGKGGKSSRMTKCNRSGTQTPGSNIGS 125
S +AG S + + ++ G N GS
Sbjct: 643 SAEAGMSKLSGEINQVWQTTKDGVGQNSGS 732
>TC10474
Length = 446
Score = 26.6 bits (57), Expect = 1.8
Identities = 11/27 (40%), Positives = 15/27 (54%)
Frame = -1
Query: 6 TPPATAAPDQIMQQRQLPFSSMKPPFL 32
TPP + P++ QQ+Q PF P L
Sbjct: 209 TPPNASTPNKTQQQQQTPFDLESDPGL 129
>TC10297 similar to UP|G6PC_SOLTU (Q43839) Glucose-6-phosphate
1-dehydrogenase, chloroplast precursor (G6PD) , partial
(19%)
Length = 547
Score = 26.2 bits (56), Expect = 2.4
Identities = 11/35 (31%), Positives = 19/35 (53%)
Frame = -2
Query: 7 PPATAAPDQIMQQRQLPFSSMKPPFLAAADYHRFA 41
P T+ P Q + +LP+++ K + DYH+ A
Sbjct: 447 PYETSLPTQREESTKLPYNTRKKKLIINIDYHKKA 343
>AV769441
Length = 594
Score = 25.8 bits (55), Expect = 3.1
Identities = 11/36 (30%), Positives = 19/36 (52%)
Frame = -2
Query: 90 SVQTPVSGKAGKGGKSSRMTKCNRSGTQTPGSNIGS 125
++Q P+S G G S +C++ QT N+G+
Sbjct: 197 TIQMPISNIDGGGYGISNNARCSKPSAQTNRRNLGT 90
>TC19076
Length = 313
Score = 25.4 bits (54), Expect = 4.1
Identities = 13/35 (37%), Positives = 19/35 (54%)
Frame = +3
Query: 1 MSGTRTPPATAAPDQIMQQRQLPFSSMKPPFLAAA 35
++G TP T AP+++ P SS PP + AA
Sbjct: 117 LTGLETP-TTTAPEELPDFAGSPVSSTVPPLVTAA 218
>AV774634
Length = 430
Score = 25.0 bits (53), Expect = 5.3
Identities = 16/44 (36%), Positives = 22/44 (49%)
Frame = +2
Query: 2 SGTRTPPATAAPDQIMQQRQLPFSSMKPPFLAAADYHRFAPDHR 45
SGT TPP T +Q++ Q P KP L +H P++R
Sbjct: 230 SGTATPPRTEPHNQVLCLGQTP----KPSTLFIFSFHN--PNNR 343
>TC15031 weakly similar to UP|Q69107 (Q69107) LAT protein, partial (27%)
Length = 997
Score = 25.0 bits (53), Expect = 5.3
Identities = 17/56 (30%), Positives = 22/56 (38%), Gaps = 7/56 (12%)
Frame = +1
Query: 5 RTPPATAAPDQIMQQR-------QLPFSSMKPPFLAAADYHRFAPDHRRNQDLETE 53
RTPPAT P Q+ +R Q P P H P R +Q+ + E
Sbjct: 649 RTPPATRLPPQVPLRRLRSNQSLQNPHHLESGPLRTRVPRHAPLPPQRIDQEEDAE 816
>CB826725
Length = 490
Score = 25.0 bits (53), Expect = 5.3
Identities = 20/70 (28%), Positives = 30/70 (42%), Gaps = 6/70 (8%)
Frame = +3
Query: 79 MTPGSTAAANSSVQTPVSGKAGKGGKSSRMTKCNRS-GTQTPGSN-----IGSPSGNNLT 132
+TP ST + + VQ + KSS +TK RS +P N + P ++
Sbjct: 153 ITPSSTPSTKALVQVSSNPSRSMVQKSSLLTKTVRSVSGSSPHQNDTVS*LPFPPSRTVS 332
Query: 133 PAGPCRYDSS 142
A CR +S
Sbjct: 333 DAASCRKTTS 362
>TC16349 homologue to UP|PRS6_SOLTU (P54778) 26S protease regulatory subunit
6B homolog, partial (65%)
Length = 901
Score = 25.0 bits (53), Expect = 5.3
Identities = 13/36 (36%), Positives = 18/36 (49%)
Frame = -3
Query: 97 GKAGKGGKSSRMTKCNRSGTQTPGSNIGSPSGNNLT 132
G A +G R+ K T+ P +GS SG+N T
Sbjct: 413 GMAVEGDGGGRLEKLAIDSTKNPNIVVGSSSGSNDT 306
>CN825172
Length = 655
Score = 25.0 bits (53), Expect = 5.3
Identities = 12/26 (46%), Positives = 15/26 (57%)
Frame = +2
Query: 74 DSGDRMTPGSTAAANSSVQTPVSGKA 99
+SGDR+T + S QTPV KA
Sbjct: 131 ESGDRLTRAAKVLEQLSGQTPVFSKA 208
>TC18115 weakly similar to UP|Q93XD4 (Q93XD4) Thaumatin-like protein,
partial (63%)
Length = 792
Score = 24.6 bits (52), Expect = 7.0
Identities = 14/48 (29%), Positives = 18/48 (37%)
Frame = +2
Query: 81 PGSTAAANSSVQTPVSGKAGKGGKSSRMTKCNRSGTQTPGSNIGSPSG 128
PG NS P++ AG G+ T CN G+ G G
Sbjct: 122 PGGGRRLNSGETWPLNLNAGSSGRIWGRTGCNFDGSGRGSCQTGDCGG 265
>TC12845 similar to UP|ILV1_BRANA (P27818) Acetolactate synthase I,
chloroplast precursor (Acetohydroxy-acid synthase I)
(ALS I) , partial (35%)
Length = 910
Score = 24.6 bits (52), Expect = 7.0
Identities = 12/31 (38%), Positives = 19/31 (60%), Gaps = 1/31 (3%)
Frame = +3
Query: 6 TPPATAAPDQIMQQRQLPFSSMKPP-FLAAA 35
TP AT++PD + P +++ PP FL +A
Sbjct: 360 TPSATSSPDMSRVESSPPKATLAPPAFLVSA 452
>TC14229 similar to UP|P93166 (P93166) SCOF-1, partial (38%)
Length = 1175
Score = 24.6 bits (52), Expect = 7.0
Identities = 18/72 (25%), Positives = 28/72 (38%)
Frame = +1
Query: 69 ADFEADSGDRMTPGSTAAANSSVQTPVSGKAGKGGKSSRMTKCNRSGTQTPGSNIGSPSG 128
A S + TP + A +++ VS G GGK + C++S P+G
Sbjct: 436 ASHRKSSSENQTPAAAAVNDNA---SVSTSTGNGGKMHECSICHKS----------FPTG 576
Query: 129 NNLTPAGPCRYD 140
L C Y+
Sbjct: 577 QALGGHKRCHYE 612
>BP055432
Length = 495
Score = 24.3 bits (51), Expect = 9.1
Identities = 10/25 (40%), Positives = 15/25 (60%)
Frame = -3
Query: 100 GKGGKSSRMTKCNRSGTQTPGSNIG 124
GKG +S ++ NR + PGS +G
Sbjct: 490 GKGARSISLSPGNRC*EEAPGSTLG 416
>TC20072 similar to UP|Q8V7C1 (Q8V7C1) ORF1 (Fragment), partial (41%)
Length = 380
Score = 24.3 bits (51), Expect = 9.1
Identities = 14/41 (34%), Positives = 18/41 (43%)
Frame = -2
Query: 80 TPGSTAAANSSVQTPVSGKAGKGGKSSRMTKCNRSGTQTPG 120
TP A A + P G A + SR + SGT +PG
Sbjct: 127 TPSLLACATVAWGAPACGLADAARQWSRHQRVLTSGTHSPG 5
>TC14550 similar to GB|AAL05900.1|15777879|AY055100 AT3g15640/MSJ11_4
{Arabidopsis thaliana;}, partial (59%)
Length = 873
Score = 24.3 bits (51), Expect = 9.1
Identities = 15/40 (37%), Positives = 24/40 (59%), Gaps = 1/40 (2%)
Frame = +1
Query: 2 SGTRTPPATAAPDQIMQ-QRQLPFSSMKPPFLAAADYHRF 40
S + +PPAT+ P+Q+++ +R+L SS L A RF
Sbjct: 193 SDSPSPPATSPPNQLVRSRRRLRMSSQS---LLATSVRRF 303
>TC15131 similar to UP|Q9FX71 (Q9FX71) T6J4.1 protein, partial (40%)
Length = 553
Score = 24.3 bits (51), Expect = 9.1
Identities = 20/68 (29%), Positives = 36/68 (52%), Gaps = 6/68 (8%)
Frame = -1
Query: 57 VKTPQLKRKSEAADFE---ADSGDRMTPGSTAAANSSVQTPVSG---KAGKGGKSSRMTK 110
+ +P+ +R+S+ + + S +R PG T+A +S+ G ++ +G SSR +
Sbjct: 529 IASPRHRRRSDRSRSR*SASPSLERGLPG-TSARSSAAGRARGGWRTRSCRGRDSSRNGR 353
Query: 111 CNRSGTQT 118
C SGT T
Sbjct: 352 CGSSGTGT 329
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.309 0.125 0.359
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,204,159
Number of Sequences: 28460
Number of extensions: 25848
Number of successful extensions: 160
Number of sequences better than 10.0: 43
Number of HSP's better than 10.0 without gapping: 159
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 160
length of query: 146
length of database: 4,897,600
effective HSP length: 82
effective length of query: 64
effective length of database: 2,563,880
effective search space: 164088320
effective search space used: 164088320
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 51 (24.3 bits)
Medicago: description of AC146557.5