BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146554.5 + phase: 0
(236 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CN825185 50 3e-07
TC18188 similar to UP|ZFP6_ARATH (Q39265) Zinc finger protein 6,... 48 1e-06
CB826842 46 6e-06
TC15789 similar to UP|O22086 (O22086) ZPT2-14, partial (37%) 44 2e-05
TC7910 homologue to UP|P93166 (P93166) SCOF-1, partial (52%) 37 0.002
TC14229 similar to UP|P93166 (P93166) SCOF-1, partial (38%) 36 0.005
TC10315 similar to UP|P93166 (P93166) SCOF-1, partial (42%) 36 0.007
TC12719 homologue to UP|ZFP1_ARATH (Q42485) Zinc finger protein ... 35 0.011
TC17034 32 0.13
BP052714 31 0.16
TC11237 similar to UP|Q43614 (Q43614) DNA-binding protein, parti... 31 0.21
TC17191 homologue to UP|Q9SL38 (Q9SL38) At2g18090 protein, parti... 30 0.48
TC17056 similar to UP|Q94BZ2 (Q94BZ2) At1g30970/F17F8_14, partia... 29 0.62
AV779581 28 1.8
BI420500 27 2.4
BP057141 27 3.1
TC9825 similar to UP|Q9ZRD8 (Q9ZRD8) GMFP5 (Fragment), partial (... 24 3.4
AV764913 27 4.0
BP079927 26 5.3
BP081323 26 5.3
>CN825185
Length = 541
Score = 50.4 bits (119), Expect = 3e-07
Identities = 30/80 (37%), Positives = 45/80 (55%), Gaps = 9/80 (11%)
Frame = +3
Query: 22 KSTSKPLENGGHSSSF---QNTLDEPLPEQKVVEFSCNYCDKKFSTSQALGGHQNAHKRE 78
K++S P+ +G +S + Q + L + + C YC K+FS+ QA+GGHQNAH+ E
Sbjct: 96 KASSSPVSSGENSITTRKRQMGMWLSLSQPGSHGYGCLYCSKRFSSVQAVGGHQNAHRTE 275
Query: 79 RVLKKMED------RRREEE 92
RV +M R RE+E
Sbjct: 276 RVESEMRQLIMMNMREREDE 335
>TC18188 similar to UP|ZFP6_ARATH (Q39265) Zinc finger protein 6, partial
(21%)
Length = 594
Score = 48.1 bits (113), Expect = 1e-06
Identities = 29/85 (34%), Positives = 44/85 (51%), Gaps = 1/85 (1%)
Frame = +3
Query: 55 CNYCDKKFSTSQALGGHQNAHKRERVLKKMEDRRREEEMDLTLRFSSFIHYQGHSYFRSA 114
C YC ++F+ SQALGGHQNAHK+ER K + + ++ + I ++
Sbjct: 315 CQYCCREFANSQALGGHQNAHKKERQQLKRAQLQATRKAAVSFVRNPII----SAFAPPP 482
Query: 115 NLHNPIGVHVTNA-FPSWIGSPYGG 138
+L +P G + A PSW+ P G
Sbjct: 483 HLLSPSGAMMVPAGTPSWVYMPPRG 557
>CB826842
Length = 637
Score = 45.8 bits (107), Expect = 6e-06
Identities = 18/50 (36%), Positives = 29/50 (58%)
Frame = +1
Query: 49 KVVEFSCNYCDKKFSTSQALGGHQNAHKRERVLKKMEDRRREEEMDLTLR 98
+V +SC +C + FS +QALGGH N H R+R K +D++++
Sbjct: 115 QVRSYSCTFCKRGFSNAQALGGHMNIHTRDRAKLKQSSEENLLSLDISMK 264
>TC15789 similar to UP|O22086 (O22086) ZPT2-14, partial (37%)
Length = 747
Score = 43.9 bits (102), Expect = 2e-05
Identities = 19/39 (48%), Positives = 29/39 (73%)
Frame = +1
Query: 51 VEFSCNYCDKKFSTSQALGGHQNAHKRERVLKKMEDRRR 89
VEF C C++KFS+ QALGGH+ +H +RV K+E++ +
Sbjct: 235 VEFECKTCNRKFSSFQALGGHRASHNHKRV--KLEEQAK 345
Score = 30.8 bits (68), Expect = 0.21
Identities = 19/65 (29%), Positives = 25/65 (38%), Gaps = 20/65 (30%)
Frame = +1
Query: 55 CNYCDKKFSTSQALGGHQNAHKRER--------------------VLKKMEDRRREEEMD 94
C+ C FS QALGGH H+ VLK++ +R +D
Sbjct: 388 CSVCGLGFSLGQALGGHMRKHRNNEGFSSSSSSSYSIKEEVAKFPVLKRLNSSKRVLCLD 567
Query: 95 LTLRF 99
L L F
Sbjct: 568 LDLNF 582
>TC7910 homologue to UP|P93166 (P93166) SCOF-1, partial (52%)
Length = 1035
Score = 37.4 bits (85), Expect = 0.002
Identities = 17/51 (33%), Positives = 30/51 (58%), Gaps = 1/51 (1%)
Frame = +3
Query: 28 LENGGHSSSFQNTLDEPLP-EQKVVEFSCNYCDKKFSTSQALGGHQNAHKR 77
L G + + + TL P P + + + C+ C+K F + QALGGH+ +H++
Sbjct: 285 LARGSTAVTPKLTLSRPAPVTAEKLSYKCSVCEKTFPSYQALGGHKASHRK 437
Score = 33.5 bits (75), Expect = 0.033
Identities = 12/21 (57%), Positives = 14/21 (66%)
Frame = +3
Query: 55 CNYCDKKFSTSQALGGHQNAH 75
C+ C K F T QALGGH+ H
Sbjct: 525 CSICQKSFPTGQALGGHKRCH 587
>TC14229 similar to UP|P93166 (P93166) SCOF-1, partial (38%)
Length = 1175
Score = 36.2 bits (82), Expect = 0.005
Identities = 18/58 (31%), Positives = 31/58 (53%)
Frame = +1
Query: 20 HTKSTSKPLENGGHSSSFQNTLDEPLPEQKVVEFSCNYCDKKFSTSQALGGHQNAHKR 77
+T + + L S+S + + P K+ C+ CDK F + QALGGH+ +H++
Sbjct: 280 NTDNNTNTLRAESSSNSQSQSPPQQQPPLKLTH-KCSVCDKAFPSYQALGGHKASHRK 450
Score = 33.1 bits (74), Expect = 0.043
Identities = 12/21 (57%), Positives = 14/21 (66%)
Frame = +1
Query: 55 CNYCDKKFSTSQALGGHQNAH 75
C+ C K F T QALGGH+ H
Sbjct: 544 CSICHKSFPTGQALGGHKRCH 606
>TC10315 similar to UP|P93166 (P93166) SCOF-1, partial (42%)
Length = 592
Score = 35.8 bits (81), Expect = 0.007
Identities = 12/27 (44%), Positives = 20/27 (73%)
Frame = +2
Query: 51 VEFSCNYCDKKFSTSQALGGHQNAHKR 77
+ + C+ CDK F + QALGGH+ +H++
Sbjct: 383 LSYKCSVCDKAFPSYQALGGHKASHRK 463
>TC12719 homologue to UP|ZFP1_ARATH (Q42485) Zinc finger protein 1, partial
(18%)
Length = 548
Score = 35.0 bits (79), Expect = 0.011
Identities = 23/68 (33%), Positives = 32/68 (46%)
Frame = +2
Query: 34 SSSFQNTLDEPLPEQKVVEFSCNYCDKKFSTSQALGGHQNAHKRERVLKKMEDRRREEEM 93
+SS N L E +V FSCNYC +KF +SQAL G + V K + +
Sbjct: 350 NSSETNPLGCSEAEPRV--FSCNYCHRKFYSSQALWGSPKCTQEGEVNSKKKSSSSSQVW 523
Query: 94 DLTLRFSS 101
+ + SS
Sbjct: 524 NTNIGLSS 547
>TC17034
Length = 519
Score = 31.6 bits (70), Expect = 0.13
Identities = 15/47 (31%), Positives = 22/47 (45%)
Frame = -3
Query: 31 GGHSSSFQNTLDEPLPEQKVVEFSCNYCDKKFSTSQALGGHQNAHKR 77
G HSS F N P+Q + + +KKF + + G QN K+
Sbjct: 451 GSHSSHFSNNHSSQFPQQHIQKPRKKSINKKFHKNNSSGSEQNIRKQ 311
>BP052714
Length = 511
Score = 31.2 bits (69), Expect = 0.16
Identities = 17/52 (32%), Positives = 25/52 (47%)
Frame = -1
Query: 23 STSKPLENGGHSSSFQNTLDEPLPEQKVVEFSCNYCDKKFSTSQALGGHQNA 74
+T P + G S+S + P+ E+SC C++ F T ALG H A
Sbjct: 343 ATPYPKQAGKASASTKQPAKPQTPKSGG-EYSCKPCNRSFKTEDALGSHXKA 191
>TC11237 similar to UP|Q43614 (Q43614) DNA-binding protein, partial (20%)
Length = 523
Score = 30.8 bits (68), Expect = 0.21
Identities = 10/22 (45%), Positives = 15/22 (67%)
Frame = +3
Query: 55 CNYCDKKFSTSQALGGHQNAHK 76
C+ C +F++ QALGGH H+
Sbjct: 60 CSICGSEFTSGQALGGHMRRHR 125
>TC17191 homologue to UP|Q9SL38 (Q9SL38) At2g18090 protein, partial (5%)
Length = 598
Score = 29.6 bits (65), Expect = 0.48
Identities = 27/87 (31%), Positives = 36/87 (41%), Gaps = 3/87 (3%)
Frame = +2
Query: 112 RSANLHNPIGVHVTNAFPSWIGSPYGGYGGVYMPNTPLATRYVMQMPNSPQATPQFG--- 168
RS P H+ S G +GG GG + TPLA R +P AT G
Sbjct: 71 RSQRTRLPPSKHMKQTSSSSSGC-FGGGGGGAVLETPLAGRPRFLFKTTPSATAGAGLSA 247
Query: 169 MTNFWGGGLNASLPIPQRSNTLGLGLF 195
M + ++S P+P RS+ L F
Sbjct: 248 MPDTPSSFFDSSKPLPLRSSDATLTTF 328
>TC17056 similar to UP|Q94BZ2 (Q94BZ2) At1g30970/F17F8_14, partial (40%)
Length = 736
Score = 29.3 bits (64), Expect = 0.62
Identities = 15/50 (30%), Positives = 22/50 (44%)
Frame = +2
Query: 48 QKVVEFSCNYCDKKFSTSQALGGHQNAHKRERVLKKMEDRRREEEMDLTL 97
QK F C+ C KK ST+ + H +E V K + E D+ +
Sbjct: 188 QKAKHFKCHVCHKKLSTAGGMSIHVLQVHKESVTKVPNAKPGRESTDIEI 337
>AV779581
Length = 427
Score = 27.7 bits (60), Expect = 1.8
Identities = 15/49 (30%), Positives = 23/49 (46%)
Frame = +2
Query: 178 NASLPIPQRSNTLGLGLFPQRNFIDQLHSHTLPLSSRDPNREGLIQANP 226
N +PIP ++ L P + + Q H+H+LP+ P G Q P
Sbjct: 233 NTKIPIPIQTLLQVLNEHPNQRY--QSHNHSLPI*QHQPLNTGTCQTLP 373
>BI420500
Length = 539
Score = 27.3 bits (59), Expect = 2.4
Identities = 31/117 (26%), Positives = 44/117 (37%), Gaps = 5/117 (4%)
Frame = +2
Query: 2 PPTSESHVEDFLTLRLGTHTKSTSKPLENG-----GHSSSFQNTLDEPLPEQKVVEFSCN 56
P TS++ + + L+ R KS +PL N G SSF L + E + SCN
Sbjct: 53 PQTSQTSLTENLSDR-----KSLDRPLNNEVASSEGSDSSFVKNLKRSVRETALSVRSCN 217
Query: 57 YCDKKFSTSQALGGHQNAHKRERVLKKMEDRRREEEMDLTLRFSSFIHYQGHSYFRS 113
+ S S H A E ++ +R F S G S+F S
Sbjct: 218 SESSRDSHSDGSSEHFFAPLSETGFSHLDAEKRAASSRSKRLFES---QMGDSFFES 379
>BP057141
Length = 466
Score = 26.9 bits (58), Expect = 3.1
Identities = 11/33 (33%), Positives = 20/33 (60%)
Frame = +2
Query: 58 CDKKFSTSQALGGHQNAHKRERVLKKMEDRRRE 90
C T +A+ GH++ H+R+++ K E+ RE
Sbjct: 332 CGADEKTVRAILGHRSVHQRQQIRKVYEELYRE 430
>TC9825 similar to UP|Q9ZRD8 (Q9ZRD8) GMFP5 (Fragment), partial (44%)
Length = 848
Score = 24.3 bits (51), Expect(2) = 3.4
Identities = 8/21 (38%), Positives = 14/21 (66%)
Frame = +2
Query: 136 YGGYGGVYMPNTPLATRYVMQ 156
YGGYGG+ + + P+ + M+
Sbjct: 371 YGGYGGMEVHHEPMYNNHYME 433
Score = 20.8 bits (42), Expect(2) = 3.4
Identities = 11/21 (52%), Positives = 11/21 (52%)
Frame = +2
Query: 121 GVHVTNAFPSWIGSPYGGYGG 141
G H A S S YGGYGG
Sbjct: 323 GQHEQGASSS--SSSYGGYGG 379
>AV764913
Length = 424
Score = 26.6 bits (57), Expect = 4.0
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = +1
Query: 167 FGMTNFWGGGLNASLPIPQRSNTLGLG 193
FG+TNF+ GL +S I + ++ G G
Sbjct: 220 FGLTNFFSAGLRSSTAIGRVASAAGTG 300
>BP079927
Length = 438
Score = 26.2 bits (56), Expect = 5.3
Identities = 16/65 (24%), Positives = 29/65 (44%), Gaps = 2/65 (3%)
Frame = +3
Query: 18 GTHTKSTSKPLENGGHSSSFQNTLDEPLPEQKVVE--FSCNYCDKKFSTSQALGGHQNAH 75
G + K KP +G +++ LPE +V + +CNYC K++ G N +
Sbjct: 216 GANPKKKRKPNASGPRTTTSIWKHFTRLPENEVQDPTAACNYCGKRYLCDSKTHGTTNLN 395
Query: 76 KRERV 80
++
Sbjct: 396 THLKI 410
>BP081323
Length = 471
Score = 26.2 bits (56), Expect = 5.3
Identities = 13/39 (33%), Positives = 21/39 (53%), Gaps = 4/39 (10%)
Frame = +3
Query: 17 LGTHTKSTSKPLE----NGGHSSSFQNTLDEPLPEQKVV 51
L TK++S + +GG+ S+F LD P+P K +
Sbjct: 246 LDIFTKTSSSSVHGLGGDGGYLSTFHQNLDAPIPVTKAI 362
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.315 0.132 0.398
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,760,256
Number of Sequences: 28460
Number of extensions: 73947
Number of successful extensions: 400
Number of sequences better than 10.0: 46
Number of HSP's better than 10.0 without gapping: 395
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 400
length of query: 236
length of database: 4,897,600
effective HSP length: 87
effective length of query: 149
effective length of database: 2,421,580
effective search space: 360815420
effective search space used: 360815420
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.5 bits)
S2: 54 (25.4 bits)
Medicago: description of AC146554.5