BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146554.10 + phase: 2 /partial
(83 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV428268 44 4e-06
AU089037 37 6e-04
BP056597 33 0.007
BP041113 31 0.046
TC7876 homologue to UP|GBLP_SOYBN (Q39836) Guanine nucleotide-bi... 29 0.13
BP050571 27 0.86
AV422822 26 1.1
TC7958 similar to UP|Q41125 (Q41125) Proline-rich 14 kDa protein... 26 1.1
TC9761 similar to UP|O65848 (O65848) Annexin, partial (55%) 25 1.9
TC12113 weakly similar to GB|CAD40580.1|21741678|OSJN00141 oj000... 25 2.5
TC14046 homologue to UP|P93769 (P93769) Elongation factor-1 alph... 25 3.3
BP030737 24 4.3
AV772319 24 4.3
AV766513 24 5.6
TC12501 similar to UP|Q9LR83 (Q9LR83) F21B7.2, partial (55%) 24 5.6
BP047703 24 5.6
AV777945 23 7.3
AV772449 23 7.3
TC19418 weakly similar to UP|Q9FZ10 (Q9FZ10) Serine threonine ki... 23 7.3
TC14085 23 9.6
>AV428268
Length = 429
Score = 44.3 bits (103), Expect = 4e-06
Identities = 27/80 (33%), Positives = 38/80 (46%)
Frame = +3
Query: 1 TGYDLLTADDNNTDVAVSELIWHRHVPLKVSILAWCLLRNRLPTKANLVARGTLAPDAQL 60
+ YD L N + +L+W VP L+W +L NR+ TK NL RG + + +
Sbjct: 18 SAYDFLHGCANAQWDNIFKLLWSVKVPSNAIALSWKVLINRVQTKVNLNRRGVVI--SNV 191
Query: 61 CVSGCWAVETAQHLFVSCPI 80
C E+ HL SCPI
Sbjct: 192CPLCSLDEESTDHLLFSCPI 251
>AU089037
Length = 245
Score = 37.0 bits (84), Expect = 6e-04
Identities = 19/65 (29%), Positives = 31/65 (47%), Gaps = 1/65 (1%)
Frame = -1
Query: 17 VSELIWHRHVPLKVSILAWCLLRNRLPTKANLVARGTLAPDAQ-LCVSGCWAVETAQHLF 75
+ + +W P AW L+ +R+ T+ NL R + + LC E++ HLF
Sbjct: 203 IFQKLWKVPAPSNAVSFAWRLILDRVQTRGNLRRRQVIQQSEEALCPMCSQCEESSSHLF 24
Query: 76 VSCPI 80
SCP+
Sbjct: 23 FSCPL 9
>BP056597
Length = 414
Score = 33.5 bits (75), Expect = 0.007
Identities = 16/51 (31%), Positives = 24/51 (46%)
Frame = +3
Query: 1 TGYDLLTADDNNTDVAVSELIWHRHVPLKVSILAWCLLRNRLPTKANLVAR 51
+ YDLL+ D ++V +W P L W + +R+ TK NL R
Sbjct: 240 SAYDLLSGLDTTIGISVFSKLWKACAPSNAVALGWRVFLDRIQTKDNLSRR 392
>BP041113
Length = 503
Score = 30.8 bits (68), Expect = 0.046
Identities = 23/75 (30%), Positives = 28/75 (36%), Gaps = 1/75 (1%)
Frame = +1
Query: 1 TGYDLLTADDNNTDVAVSELIWHRHVPLKVSILAWCLLRNRLPTKANLVARGTLAPDAQL 60
+ Y L A + IW P V AW L RLPT NL+ R + A
Sbjct: 271 SAYSFLQAPTLADTDPIFATIWRTVAP-SVKAFAWRCLLGRLPTYDNLIKRQVVVDPAMT 447
Query: 61 CVSGCWA-VETAQHL 74
C VE+ HL
Sbjct: 448 VCKFCQGEVESVTHL 492
>TC7876 homologue to UP|GBLP_SOYBN (Q39836) Guanine nucleotide-binding
protein beta subunit-like protein, complete
Length = 1379
Score = 29.3 bits (64), Expect = 0.13
Identities = 18/43 (41%), Positives = 21/43 (47%)
Frame = +3
Query: 22 WHRHVPLKVSILAWCLLRNRLPTKANLVARGTLAPDAQLCVSG 64
W R V KV L C LRN L + V ++PD LC SG
Sbjct: 582 WDRTV--KVWNLTNCKLRNTLAGHSGYVNTVAVSPDGSLCASG 704
>BP050571
Length = 227
Score = 26.6 bits (57), Expect = 0.86
Identities = 12/22 (54%), Positives = 15/22 (67%)
Frame = -3
Query: 42 LPTKANLVARGTLAPDAQLCVS 63
LP+ +NL RG L DAQ CV+
Sbjct: 186 LPSISNLFQRGPLIVDAQYCVN 121
>AV422822
Length = 459
Score = 26.2 bits (56), Expect = 1.1
Identities = 15/39 (38%), Positives = 23/39 (58%)
Frame = +2
Query: 14 DVAVSELIWHRHVPLKVSILAWCLLRNRLPTKANLVARG 52
DV VS ++HRHVPL ++ + +L + L +N V G
Sbjct: 2 DVPVS--VFHRHVPLTTTLPSIIILSSPLSPISNSVPAG 112
>TC7958 similar to UP|Q41125 (Q41125) Proline-rich 14 kDa protein, partial
(92%)
Length = 695
Score = 26.2 bits (56), Expect = 1.1
Identities = 12/24 (50%), Positives = 18/24 (75%)
Frame = +2
Query: 43 PTKANLVARGTLAPDAQLCVSGCW 66
P ++NLVA+GTL+ + +CV CW
Sbjct: 206 PPQSNLVAQGTLS--S*VCVLMCW 271
>TC9761 similar to UP|O65848 (O65848) Annexin, partial (55%)
Length = 558
Score = 25.4 bits (54), Expect = 1.9
Identities = 9/26 (34%), Positives = 16/26 (60%)
Frame = +2
Query: 46 ANLVARGTLAPDAQLCVSGCWAVETA 71
+N +R +L +LC++GCW + A
Sbjct: 218 SNASSRSSLVTLRKLCIAGCWNLPIA 295
>TC12113 weakly similar to GB|CAD40580.1|21741678|OSJN00141 oj000126_13.2
{Oryza sativa (japonica cultivar-group);} , partial
(40%)
Length = 484
Score = 25.0 bits (53), Expect = 2.5
Identities = 12/24 (50%), Positives = 14/24 (58%)
Frame = +2
Query: 40 NRLPTKANLVARGTLAPDAQLCVS 63
N L + NL RG L DAQ CV+
Sbjct: 230 NHLTSIRNLFQRGQLIVDAQYCVN 301
>TC14046 homologue to UP|P93769 (P93769) Elongation factor-1 alpha, partial
(84%)
Length = 1506
Score = 24.6 bits (52), Expect = 3.3
Identities = 21/60 (35%), Positives = 28/60 (46%), Gaps = 1/60 (1%)
Frame = +2
Query: 5 LLTADDNNTDVAVSELIWHRHVPLKVSILAWCLLRNRLPTKANLVARGTLAP-DAQLCVS 63
LLT + D S +H + ++ +L CLL P NLV LAP D QL +S
Sbjct: 737 LLTRSTSQRDPQTSL*GFHCRMSTRLVVLVLCLLDVLRPVS*NLVCWCLLAPMD*QLKLS 916
>BP030737
Length = 336
Score = 24.3 bits (51), Expect = 4.3
Identities = 9/20 (45%), Positives = 15/20 (75%)
Frame = +2
Query: 28 LKVSILAWCLLRNRLPTKAN 47
+K+S+ AW LLR+ L +A+
Sbjct: 257 MKISVQAWFLLRDTLAVRAH 316
>AV772319
Length = 588
Score = 24.3 bits (51), Expect = 4.3
Identities = 10/24 (41%), Positives = 16/24 (66%)
Frame = -3
Query: 31 SILAWCLLRNRLPTKANLVARGTL 54
S++ C+LR+R+P +NLV L
Sbjct: 214 SLVILCVLRSRMPRLSNLVGAEVL 143
>AV766513
Length = 456
Score = 23.9 bits (50), Expect = 5.6
Identities = 9/16 (56%), Positives = 10/16 (62%)
Frame = -1
Query: 59 QLCVSGCWAVETAQHL 74
QLC CWAV +Q L
Sbjct: 333 QLCSQHCWAVAVSQFL 286
>TC12501 similar to UP|Q9LR83 (Q9LR83) F21B7.2, partial (55%)
Length = 1062
Score = 23.9 bits (50), Expect = 5.6
Identities = 13/41 (31%), Positives = 20/41 (48%)
Frame = -1
Query: 15 VAVSELIWHRHVPLKVSILAWCLLRNRLPTKANLVARGTLA 55
++ EL WHR WC L++R+ + + GTLA
Sbjct: 429 ISKCELPWHRK---------WCSLQHRMAEREFSSSFGTLA 334
>BP047703
Length = 586
Score = 23.9 bits (50), Expect = 5.6
Identities = 12/24 (50%), Positives = 14/24 (58%)
Frame = -1
Query: 40 NRLPTKANLVARGTLAPDAQLCVS 63
N L + NL RG L DAQ CV+
Sbjct: 298 NHLTSIRNLFQRGQLIVDAQ*CVN 227
>AV777945
Length = 279
Score = 23.5 bits (49), Expect = 7.3
Identities = 11/36 (30%), Positives = 16/36 (43%)
Frame = +1
Query: 19 ELIWHRHVPLKVSILAWCLLRNRLPTKANLVARGTL 54
+ +W P LAW +L +L TK L R +
Sbjct: 154 DFLWASKAPSNSLALAWNVLIKKLQTKDELRKRNVI 261
>AV772449
Length = 353
Score = 23.5 bits (49), Expect = 7.3
Identities = 9/23 (39%), Positives = 12/23 (52%)
Frame = +2
Query: 14 DVAVSELIWHRHVPLKVSILAWC 36
DV E+IW VP+K + C
Sbjct: 125 DVGEKEIIWVELVPIKTQVQVAC 193
>TC19418 weakly similar to UP|Q9FZ10 (Q9FZ10) Serine threonine kinase
homolog COK-4, partial (11%)
Length = 523
Score = 23.5 bits (49), Expect = 7.3
Identities = 8/20 (40%), Positives = 11/20 (55%)
Frame = -1
Query: 57 DAQLCVSGCWAVETAQHLFV 76
+ QLC+ CW E H+ V
Sbjct: 259 EIQLCLPPCWQAEQKSHVDV 200
>TC14085
Length = 1723
Score = 23.1 bits (48), Expect = 9.6
Identities = 10/25 (40%), Positives = 16/25 (64%)
Frame = +1
Query: 32 ILAWCLLRNRLPTKANLVARGTLAP 56
++ C L + L + A ++ARGTL P
Sbjct: 1426 LIVLCFLLDLLGSSALILARGTLFP 1500
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.324 0.137 0.456
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,754,189
Number of Sequences: 28460
Number of extensions: 22772
Number of successful extensions: 188
Number of sequences better than 10.0: 42
Number of HSP's better than 10.0 without gapping: 188
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 188
length of query: 83
length of database: 4,897,600
effective HSP length: 59
effective length of query: 24
effective length of database: 3,218,460
effective search space: 77243040
effective search space used: 77243040
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 48 (23.1 bits)
Medicago: description of AC146554.10