BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146553.5 - phase: 1 /pseudo
(469 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AW720399 242 8e-65
TC18404 similar to UP|FUT1_PEA (Q9M5Q1) Galactoside 2-alpha-L-fu... 188 2e-48
BP050858 155 1e-38
BP045931 128 2e-30
TC12891 similar to UP|FUT1_PEA (Q9M5Q1) Galactoside 2-alpha-L-fu... 84 6e-17
BP073332 55 5e-15
BP077512 28 2.5
TC17194 UP|CAE02590 (CAE02590) Nod-factor receptor 1b, complete 28 2.5
TC16064 similar to UP|Q93VC9 (Q93VC9) At1g02300/T6A9_10, partial... 28 3.2
BP059885 28 3.2
AV769424 28 4.2
TC8595 similar to PIR|T09390|T09390 21K protein precursor - alfa... 28 4.2
AV412603 28 4.2
CN825206 28 4.2
TC17016 homologue to UP|Q8RXD0 (Q8RXD0) Auxilin-like protein (At... 27 5.5
TC13145 27 5.5
BP068300 27 5.5
TC12278 weakly similar to GB|AAP37794.1|30725544|BT008435 At3g63... 27 5.5
TC19281 similar to UP|Q9LSG4 (Q9LSG4) Genomic DNA, chromosome 3,... 27 7.1
BP050331 27 7.1
>AW720399
Length = 564
Score = 242 bits (618), Expect = 8e-65
Identities = 113/166 (68%), Positives = 136/166 (81%), Gaps = 3/166 (1%)
Frame = +1
Query: 1 DEKEKLLDGLLVSGFDEASCVSRLQSHFYHKPSPHKPSPYLISKLRKYEELHRRCGPNTR 60
++KEKLL GLL SGFDEASC+SRLQSH Y KPSPH PSPYLISKLR YE++HRRCGPNTR
Sbjct: 31 NDKEKLLHGLLASGFDEASCISRLQSHLYRKPSPHNPSPYLISKLRSYEQIHRRCGPNTR 210
Query: 61 AYNEDMKKITKS--KKNGTSAATTCKYIIWLPANGLGNQIISMASSFLYALLTGRVMLVQ 118
AY+ M KI K+ SA+ CKYI+W PANGL NQ++S+A++FLYA+LT RV+LV+
Sbjct: 211 AYHRSMTKIVKNHGASASASASAMCKYIVWTPANGLANQMLSIAATFLYAILTDRVLLVK 390
Query: 119 FGKDKEGLFCEPFLNSTWLLPEKSPFWN-AEKVQTYQSTIKMGGAN 163
G+DK GLFCEPFLNSTW+LPEKSPFWN ++VQTYQS ++ AN
Sbjct: 391 IGEDKHGLFCEPFLNSTWILPEKSPFWNVGQQVQTYQSILQKNMAN 528
>TC18404 similar to UP|FUT1_PEA (Q9M5Q1) Galactoside
2-alpha-L-fucosyltransferase (Xyloglucan
alpha-(1,2)-fucosyltransferase) (PsFT1) , partial (35%)
Length = 597
Score = 188 bits (477), Expect = 2e-48
Identities = 98/199 (49%), Positives = 133/199 (66%)
Frame = +2
Query: 200 IPLLFLEAGQYFVPSFFMTPIFKKELNKMFPEITSTFHHLGRYLFHPSNEAWELITSFYQ 259
+P L ++ YFVPS F+ P +++ELN +FP+ + FH LGRY+ HP+N W L+ +YQ
Sbjct: 8 VPWLVMKTDNYFVPSLFLMPSYEQELNDLFPKKATVFHFLGRYMLHPTNNVWGLVVRYYQ 187
Query: 260 QNLAKANERIGLQIRVVDPKLTPHQVVMNQILNCTLGNKLLPKVLGTKNIYLSSSDKNKK 319
LAK +ER+G+QIRV P+ P Q V++QIL CTL LLP V +N +SSS K
Sbjct: 188 AYLAKVDERVGIQIRVFVPEPGPFQHVLDQILACTLKENLLPDVDREQN-NISSSGLPKS 364
Query: 320 IMKAVLVSSLYRHYGENLKMTYMNKSTVSGEVIEVYQPSGEEQQKFNDNKHNMKAWVDIY 379
KAVL++SL Y E ++ Y TV+GEV+ V+QPS EE Q+ HN+KAW ++Y
Sbjct: 365 --KAVLMTSLSSGYFEKVRDMYWEYPTVTGEVVGVFQPSHEEYQQTQKQMHNLKAWAEMY 538
Query: 380 LLSLSDVLVTTYQSTFGYV 398
LLSL+DVLVT+ STFGYV
Sbjct: 539 LLSLTDVLVTSSWSTFGYV 595
>BP050858
Length = 548
Score = 155 bits (392), Expect = 1e-38
Identities = 83/184 (45%), Positives = 113/184 (61%), Gaps = 6/184 (3%)
Frame = -3
Query: 4 EKLLDGLLVSGFDEASCVSRLQSHFYHKPSPHKPSPYLISKLRKYEELHRRCGPNTRAYN 63
+KLL GLL GFDE SC+SR QS YHK KPS YLIS+LRKYE LH+ CGP T +YN
Sbjct: 546 DKLLGGLLADGFDEKSCLSRYQSVMYHKGLSGKPSSYLISRLRKYEALHKECGPYTESYN 367
Query: 64 EDMKKITKSKKNGTSAATTCKYIIWLPANGLGNQIISMASSFLYALLTGRVMLVQFGKDK 123
+ +K++ + + + CKY++W+ +GLGN+I+++AS+FLYALLT RV+LV G D
Sbjct: 366 KTVKQL---RSGLFTESPACKYVVWVSFSGLGNRILTLASAFLYALLTNRVLLVXPGVDM 196
Query: 124 EGLFCEPFLNSTWLLPEKSPF------WNAEKVQTYQSTIKMGGANTLNEDFPSALHVNL 177
LFCEPF +++W LP P ++ E Q Y +K T P A+
Sbjct: 195 IDLFCEPFPDTSWFLPPDFPLNSQLNNFSKESRQCYGKMLKEKSITT-----PRAIFCLP 31
Query: 178 GYSP 181
+SP
Sbjct: 30 SFSP 19
>BP045931
Length = 468
Score = 128 bits (322), Expect = 2e-30
Identities = 63/140 (45%), Positives = 93/140 (66%), Gaps = 4/140 (2%)
Frame = -1
Query: 334 GENLKMTYMNKSTVSGE-VIEVYQPSGEEQQKFNDNKHNMKAWVDIYLLSLSDVLVTTYQ 392
GE L+ Y+ +TV+ + VIEV+QPS EE+ N++ +++AW +IYLLSL D L+T+ Q
Sbjct: 468 GEKLRTMYLTNTTVTRDKVIEVFQPSHEERPNSNNDTPHIQAWTEIYLLSLCDALITSSQ 289
Query: 393 STFGYVAKALGNSRPWIL---YNPVYSNEICEREFTLEPCYHYPPLHYCNGKPIEDVGSS 449
STFGYVA +LG +PW+L Y + C+R +LEPC+HYPP + C D S
Sbjct: 288 STFGYVAHSLGGMKPWLLRRAYGETLPDPPCKRVKSLEPCFHYPPQYDCRDHRTVDFTSL 109
Query: 450 FPYIRHCKDYTFGVKLVNAS 469
F Y++ C+D T G++LV+A+
Sbjct: 108 FHYLKPCEDVTSGLRLVHAN 49
>TC12891 similar to UP|FUT1_PEA (Q9M5Q1) Galactoside
2-alpha-L-fucosyltransferase (Xyloglucan
alpha-(1,2)-fucosyltransferase) (PsFT1) , partial (21%)
Length = 360
Score = 83.6 bits (205), Expect = 6e-17
Identities = 40/118 (33%), Positives = 64/118 (53%)
Frame = +3
Query: 131 FLNSTWLLPEKSPFWNAEKVQTYQSTIKMGGANTLNEDFPSALHVNLGYSPTSEERFFHC 190
FL + L ++ P +N + +K N PS ++++L + +++ F C
Sbjct: 6 FLPPDFPLNDQFPSFNQNSDHCHGKMLKKKSIK--NSKVPSFVYLHLAHDYDDDDKLFFC 179
Query: 191 DHNQFLLSNIPLLFLEAGQYFVPSFFMTPIFKKELNKMFPEITSTFHHLGRYLFHPSN 248
D +Q L +P L + YFVPS F+ P F++EL+ +FP + FH LGRYLFHP+N
Sbjct: 180 DEDQLFLHKVPWLVVRTDNYFVPSLFLMPSFQQELSDLFPNKETVFHFLGRYLFHPTN 353
>BP073332
Length = 391
Score = 55.5 bits (132), Expect(2) = 5e-15
Identities = 20/49 (40%), Positives = 34/49 (68%)
Frame = -2
Query: 419 ICEREFTLEPCYHYPPLHYCNGKPIEDVGSSFPYIRHCKDYTFGVKLVN 467
+ +R ++EPC+H PP + C K D G+ P++RHC+D ++G+KLV+
Sbjct: 285 LAQRAMSIEPCFHAPPFYDCKAKRGIDTGALVPHVRHCEDMSWGLKLVD 139
Score = 42.0 bits (97), Expect(2) = 5e-15
Identities = 18/27 (66%), Positives = 21/27 (77%)
Frame = -1
Query: 387 LVTTYQSTFGYVAKALGNSRPWILYNP 413
LVT+ STFGYVA+ LG +PWILY P
Sbjct: 391 LVTSSWSTFGYVAQGLGGLKPWILYQP 311
>BP077512
Length = 513
Score = 28.5 bits (62), Expect = 2.5
Identities = 13/26 (50%), Positives = 19/26 (73%)
Frame = +1
Query: 312 SSSDKNKKIMKAVLVSSLYRHYGENL 337
S+SDKN K M +L SS++R + EN+
Sbjct: 382 SNSDKNFKEMNLLLQSSIHRLFAENI 459
>TC17194 UP|CAE02590 (CAE02590) Nod-factor receptor 1b, complete
Length = 2193
Score = 28.5 bits (62), Expect = 2.5
Identities = 16/57 (28%), Positives = 26/57 (45%)
Frame = +1
Query: 249 EAWELITSFYQQNLAKANERIGLQIRVVDPKLTPHQVVMNQILNCTLGNKLLPKVLG 305
+ +E I + Y NL + + + DPK P +N +NC+ GN + K G
Sbjct: 442 DTYETIANLYYANLTTVD--LLKRFNSYDPKNIPVNAKVNVTVNCSCGNSQVSKDYG 606
>TC16064 similar to UP|Q93VC9 (Q93VC9) At1g02300/T6A9_10, partial (41%)
Length = 558
Score = 28.1 bits (61), Expect = 3.2
Identities = 22/75 (29%), Positives = 34/75 (45%)
Frame = +2
Query: 169 FPSALHVNLGYSPTSEERFFHCDHNQFLLSNIPLLFLEAGQYFVPSFFMTPIFKKELNKM 228
FPS+L SP++ R +HN FLLS++ L+F + TP L +
Sbjct: 26 FPSSLPC-FWLSPSTISRSLKQNHNSFLLSSLTLVFFRS--QLRKRLTKTPGQDGRLLSV 196
Query: 229 FPEITSTFHHLGRYL 243
T+ H+L +L
Sbjct: 197 LVSPTTQLHNLSAFL 241
>BP059885
Length = 399
Score = 28.1 bits (61), Expect = 3.2
Identities = 12/28 (42%), Positives = 16/28 (56%)
Frame = +2
Query: 161 GANTLNEDFPSALHVNLGYSPTSEERFF 188
G NT+ +DFPS L +S TS F+
Sbjct: 137 GHNTIKQDFPSKPXQQLAHSSTSHHTFY 220
>AV769424
Length = 483
Score = 27.7 bits (60), Expect = 4.2
Identities = 14/38 (36%), Positives = 21/38 (54%), Gaps = 1/38 (2%)
Frame = +2
Query: 212 VPSFFMTPIFKKELNKMFPE-ITSTFHHLGRYLFHPSN 248
+P + IF + L+ FP + FHHLG + FH S+
Sbjct: 47 IPRISLFFIFNESLDL*FPNWFITQFHHLGLHHFHQSS 160
>TC8595 similar to PIR|T09390|T09390 21K protein precursor - alfalfa
{Medicago sativa;}, partial (93%)
Length = 811
Score = 27.7 bits (60), Expect = 4.2
Identities = 14/29 (48%), Positives = 17/29 (58%), Gaps = 2/29 (6%)
Frame = +3
Query: 27 HFYHKPSPHKPSPYL--ISKLRKYEELHR 53
H H PSPH P P L +S RK +LH+
Sbjct: 33 HGKHTPSPHHPVPPLRPVSHHRKPHQLHQ 119
>AV412603
Length = 336
Score = 27.7 bits (60), Expect = 4.2
Identities = 18/68 (26%), Positives = 25/68 (36%), Gaps = 15/68 (22%)
Frame = +2
Query: 1 DEKEKLLDGLLVSGFDEASCVSRLQSHFYHKPSPHKP---------------SPYLISKL 45
D K+ + G+DEA S L S HKP P +L+
Sbjct: 98 DFKDAVRQSARSLGYDEAKLASILASFIIHKPPQRSPFYKAALKTLESIGELEQFLLKHR 277
Query: 46 RKYEELHR 53
+ Y +LHR
Sbjct: 278 KDYTDLHR 301
>CN825206
Length = 679
Score = 27.7 bits (60), Expect = 4.2
Identities = 17/60 (28%), Positives = 29/60 (48%), Gaps = 4/60 (6%)
Frame = -2
Query: 192 HNQFLLSNIPLLFLEAGQYFVPSFFMTPIFKKELNKMF----PEITSTFHHLGRYLFHPS 247
HN+ + N PL G ++P+F T I + +L + F + + FH+L + H S
Sbjct: 327 HNELWMPNHPLSIQREGPGWLPAFLWTLIPQLQLARFFVLEIEVLLTHFHYLH*RMLHHS 148
>TC17016 homologue to UP|Q8RXD0 (Q8RXD0) Auxilin-like protein (At4g12780),
partial (15%)
Length = 634
Score = 27.3 bits (59), Expect = 5.5
Identities = 10/19 (52%), Positives = 13/19 (67%), Gaps = 1/19 (5%)
Frame = +3
Query: 428 PCYHY-PPLHYCNGKPIED 445
PCY Y PPLH+C + +D
Sbjct: 276 PCYSYLPPLHFCFDRSYDD 332
>TC13145
Length = 661
Score = 27.3 bits (59), Expect = 5.5
Identities = 17/57 (29%), Positives = 25/57 (43%), Gaps = 3/57 (5%)
Frame = +1
Query: 89 LPANGLGNQIISMASSFLYALLTGRVMLVQFGKDKEGLFCEPFLNSTW---LLPEKS 142
LP G+G QI M A+ GRV++ + + C+ S+W PE S
Sbjct: 157 LPGFGIGAQIAGMCGLLGIAISEGRVLVANYYNRADHDGCQGSSRSSWSCYFFPETS 327
>BP068300
Length = 452
Score = 27.3 bits (59), Expect = 5.5
Identities = 10/36 (27%), Positives = 19/36 (52%)
Frame = +1
Query: 396 GYVAKALGNSRPWILYNPVYSNEICEREFTLEPCYH 431
G+ AK L + + W+LY+ ++C + C+H
Sbjct: 244 GHKAKCLSSCQVWLLYSRNSYYKVCAGSLKMVECWH 351
>TC12278 weakly similar to GB|AAP37794.1|30725544|BT008435 At3g63460
{Arabidopsis thaliana;}, partial (18%)
Length = 1034
Score = 27.3 bits (59), Expect = 5.5
Identities = 26/120 (21%), Positives = 49/120 (40%), Gaps = 14/120 (11%)
Frame = +2
Query: 96 NQIISMASSFLYALLTGRVMLVQFGKDKEGLFCEPFLNS-TW----LLPEKSPFWNAEKV 150
N ++S +S F A+ G L++ DK+ E TW ++ E + +
Sbjct: 116 NSLVSRSSEFEAAIQNGERSLLRVLCDKKTEESESEEEKETWGFLKVMFEDDGTARTKLL 295
Query: 151 QTYQSTIKMGGANTLNEDFPSALHV---------NLGYSPTSEERFFHCDHNQFLLSNIP 201
+ + +T+NED ++ N G+ PT+E F D+ + +N+P
Sbjct: 296 THLGFDVPIEAKDTINEDLSQEVNALGLEDTAVNNAGHVPTTETAIFSTDNGEDFFNNLP 475
>TC19281 similar to UP|Q9LSG4 (Q9LSG4) Genomic DNA, chromosome 3, P1 clone:
MJL12, partial (8%)
Length = 549
Score = 26.9 bits (58), Expect = 7.1
Identities = 15/33 (45%), Positives = 18/33 (54%)
Frame = -1
Query: 288 NQILNCTLGNKLLPKVLGTKNIYLSSSDKNKKI 320
N +LN K + T+NIY SSS KN KI
Sbjct: 396 NNLLN*LKYKKKHHHYIDTQNIYFSSSKKNTKI 298
>BP050331
Length = 519
Score = 26.9 bits (58), Expect = 7.1
Identities = 15/63 (23%), Positives = 29/63 (45%)
Frame = -1
Query: 17 EASCVSRLQSHFYHKPSPHKPSPYLISKLRKYEELHRRCGPNTRAYNEDMKKITKSKKNG 76
E S +R +S H P+PH+ SP + K R R + + + +++ +G
Sbjct: 513 ENSTDTRSRSFPEHNPNPHRRSPSAPDRTTKPRREKRGQTYGARTRRDSYQDVAEARASG 334
Query: 77 TSA 79
T++
Sbjct: 333 TAS 325
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.319 0.136 0.414
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,864,994
Number of Sequences: 28460
Number of extensions: 159410
Number of successful extensions: 927
Number of sequences better than 10.0: 43
Number of HSP's better than 10.0 without gapping: 916
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 921
length of query: 469
length of database: 4,897,600
effective HSP length: 94
effective length of query: 375
effective length of database: 2,222,360
effective search space: 833385000
effective search space used: 833385000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 57 (26.6 bits)
Medicago: description of AC146553.5