BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146553.15 - phase: 0
(880 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC11730 346 8e-96
TC11943 similar to UP|Q9ZVT8 (Q9ZVT8) F15K9.3, partial (7%) 151 6e-42
CN824962 143 1e-34
AU088798 102 3e-22
BP064914 96 2e-20
AI967599 75 5e-14
AV770658 74 1e-13
TC14881 50 2e-06
TC15471 48 6e-06
AV779203 47 1e-05
AV766525 45 7e-05
TC8961 similar to GB|BAB71852.1|16902294|AB062739 kinesin-relate... 42 3e-04
BP030839 41 0.001
TC17849 41 0.001
TC9015 similar to UP|Q9SS90 (Q9SS90) F4P13.19 protein, partial (... 39 0.004
TC11541 weakly similar to UP|O64756 (O64756) At2g34920 protein, ... 39 0.004
TC10929 similar to UP|Q94AX6 (Q94AX6) AT3g23280/K14B15_17, parti... 39 0.005
TC13326 38 0.006
BP077155 37 0.010
CB826758 37 0.014
>TC11730
Length = 914
Score = 346 bits (888), Expect = 8e-96
Identities = 184/250 (73%), Positives = 201/250 (79%)
Frame = +2
Query: 1 MASLVVSGNSQMSSSVSVQEKGSRNKRKFRADPPLGESSKSISSLQHESLSYEFSAEKVE 60
MAS+V SG+SQMS SVSV EKGSRNKRKFRADPPLGE S S Q ESLSYEFSAE +
Sbjct: 179 MASMVASGSSQMSPSVSVPEKGSRNKRKFRADPPLGEPSIITHSPQQESLSYEFSAENFQ 358
Query: 61 ITPCFGPVTASDLCSVSHGCSDGLKLDLGLSSPAVSSEVRLCQPKEELEVVESHGADWSD 120
G +TASD+ H SDGLKL LGLS P VSS V L +PKEELEV E H ADWSD
Sbjct: 359 KALVHGQLTASDMYQ-DH--SDGLKLGLGLSIPVVSSGVILSEPKEELEVDECHDADWSD 529
Query: 121 HTETQLQELVLSNLQTIFKSAIKKIVACGYTEDVATKAMLRPGICYGCKDTVSNIVDNTL 180
TE QL+ELVLSNL +FKSAIKKIV CGY EDVATKA+LR GICYGCKDT+SNIVDN +
Sbjct: 530 -TEAQLEELVLSNLDAVFKSAIKKIVGCGYPEDVATKAILRSGICYGCKDTISNIVDNII 706
Query: 181 AFLRNGQEFDPSREHYFKDLAELQNYILAELVCVLQEVRPFFSFGDAMWCLLISDMNVSH 240
FLRNGQE DP REHYF+DL +L+ YILAE+VCVL+EVRPFF+ GDAMWCLLI DMNVSH
Sbjct: 707 GFLRNGQEIDP-REHYFEDLVQLEKYILAEMVCVLREVRPFFTAGDAMWCLLICDMNVSH 883
Query: 241 ACAMDGDPLS 250
ACAMDGD LS
Sbjct: 884 ACAMDGDTLS 913
>TC11943 similar to UP|Q9ZVT8 (Q9ZVT8) F15K9.3, partial (7%)
Length = 543
Score = 151 bits (381), Expect(2) = 6e-42
Identities = 83/122 (68%), Positives = 93/122 (76%)
Frame = +3
Query: 737 IKLEAEKELRRYKDDIQKLEKEIAQIRQKSDSSKIAALKRGIDGSYAGSFKDTKKGSGFE 796
IKL+AEK ++Y+DDI KLEKEI+ +R KSDSSKIAAL++GIDG YA D K + F
Sbjct: 3 IKLKAEKNWQKYRDDICKLEKEISLLRLKSDSSKIAALRKGIDGCYASRCMDMKNDTSF- 179
Query: 797 EPHTASISELVQKLNNFSMNGGGVKRERECVMCLSEEMSVVFLPCAHQVVCTKCNELHEK 856
ISELV +SM GG VKRERECVMCLSEEMSVVFLPCAHQVVCT CNELHEK
Sbjct: 180 ------ISELVI---GYSMTGG-VKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEK 329
Query: 857 QG 858
QG
Sbjct: 330 QG 335
Score = 37.7 bits (86), Expect(2) = 6e-42
Identities = 15/24 (62%), Positives = 20/24 (82%)
Frame = +1
Query: 857 QGMQDCPSCRSPIQERISVRYART 880
+ + DCPSCRSPIQ+RI+VR+ T
Sbjct: 331 KALLDCPSCRSPIQQRIAVRFPCT 402
>CN824962
Length = 673
Score = 143 bits (360), Expect = 1e-34
Identities = 72/131 (54%), Positives = 102/131 (76%)
Frame = +1
Query: 514 DKSMRQWLPQDRKDELILKMVPRVRELQNELQEWTEWANQKVMQAARRLSKDKAELKTLR 573
D++M + + D+KDE+I+ + ++++L+ +++E EWA++K MQAAR+LS D ELK LR
Sbjct: 283 DENM-ELVADDQKDEVIVTLFHQIKDLEKQVKEVKEWAHKKAMQAARKLSNDLTELKMLR 459
Query: 574 QEKEEVERLKKEKQCLEENTMKKLSEMENALGKAGGQVERANTAVRKLEMENAALRKEME 633
E+EE ++LKK KQ LE+ TMK+LSEMENAL KA GQV+RAN AVR+LE ENA ++ EME
Sbjct: 460 MEREETQKLKKGKQALEDTTMKRLSEMENALRKASGQVDRANAAVRRLETENAEIKAEME 639
Query: 634 AAKLRAVESAT 644
A+KL A ES +
Sbjct: 640 ASKLSASESVS 672
Score = 39.3 bits (90), Expect = 0.003
Identities = 28/115 (24%), Positives = 60/115 (51%), Gaps = 13/115 (11%)
Frame = +1
Query: 672 EELMTEKNKLAHISKESKQAEVQAEQFEAKRRQAAKKTEELLSMVSSIRKEREQIEELAR 731
E ++T +++ + K+ K+ + A + K QAA+K L+ + +R ERE+ ++L +
Sbjct: 322 EVIVTLFHQIKDLEKQVKEVKEWAHK---KAMQAARKLSNDLTELKMLRMEREETQKLKK 492
Query: 732 TKE-------------ERIKLEAEKELRRYKDDIQKLEKEIAQIRQKSDSSKIAA 773
K+ E +A ++ R +++LE E A+I+ + ++SK++A
Sbjct: 493 GKQALEDTTMKRLSEMENALRKASGQVDRANAAVRRLETENAEIKAEMEASKLSA 657
>AU088798
Length = 244
Score = 102 bits (253), Expect = 3e-22
Identities = 56/79 (70%), Positives = 62/79 (77%)
Frame = +2
Query: 704 QAAKKTEELLSMVSSIRKEREQIEELARTKEERIKLEAEKELRRYKDDIQKLEKEIAQIR 763
QAAK ELL SS RKEREQIEE A++KEE IKL+AE L+RY DDIQKLEKEIAQ+
Sbjct: 2 QAAKAKXELLLQASSXRKEREQIEESAKSKEEXIKLKAEXNLQRYGDDIQKLEKEIAQLX 181
Query: 764 QKSDSSKIAALKRGIDGSY 782
K+DSSKIAAL IDGSY
Sbjct: 182 LKTDSSKIAALXIXIDGSY 238
>BP064914
Length = 436
Score = 96.3 bits (238), Expect = 2e-20
Identities = 58/93 (62%), Positives = 63/93 (67%)
Frame = +3
Query: 1 MASLVVSGNSQMSSSVSVQEKGSRNKRKFRADPPLGESSKSISSLQHESLSYEFSAEKVE 60
MAS+V SG+SQMS SVSV EKGSRNKRKFRADPPLGE S S Q ESLSYEFSAE +
Sbjct: 138 MASMVASGSSQMSPSVSVPEKGSRNKRKFRADPPLGEPSIITHSPQQESLSYEFSAENFQ 317
Query: 61 ITPCFGPVTASDLCSVSHGCSDGLKLDLGLSSP 93
G +T SD+ H SD LKL LG P
Sbjct: 318 KALVHGQLTCSDMYQ-DH--SDWLKLGLGFVYP 407
>AI967599
Length = 412
Score = 75.1 bits (183), Expect = 5e-14
Identities = 33/70 (47%), Positives = 49/70 (69%), Gaps = 1/70 (1%)
Frame = +1
Query: 807 VQKLNNFS-MNGGGVKRERECVMCLSEEMSVVFLPCAHQVVCTKCNELHEKQGMQDCPSC 865
+Q+L+N M+ V REC++C+ +E+SVVFLPCAHQV+C C++ + ++G CP C
Sbjct: 16 LQELDNLEDMSEKEVSSNRECIICMKDEVSVVFLPCAHQVMCASCSDEYGRKGKAACPCC 195
Query: 866 RSPIQERISV 875
R IQ+RI V
Sbjct: 196 RVQIQQRIRV 225
>AV770658
Length = 560
Score = 73.9 bits (180), Expect = 1e-13
Identities = 41/78 (52%), Positives = 51/78 (64%)
Frame = +2
Query: 595 KKLSEMENALGKAGGQVERANTAVRKLEMENAALRKEMEAAKLRAVESATNFQEVSKREK 654
++LSEME+A K G++ L +EMEAAKL A E+AT+ QEVS+REK
Sbjct: 11 EELSEMESACLKLVGRLREQMLLSGNLRGRRLH*EREMEAAKLHATETATSCQEVSRREK 190
Query: 655 KTQMKFQSWENQKSLLQE 672
KTQMKFQSWE QKS +E
Sbjct: 191 KTQMKFQSWEKQKSWFRE 244
Score = 61.6 bits (148), Expect = 5e-10
Identities = 44/116 (37%), Positives = 58/116 (49%)
Frame = +1
Query: 604 LGKAGGQVERANTAVRKLEMENAALRKEMEAAKLRAVESATNFQEVSKREKKTQMKFQSW 663
L KA GQV+RAN AVRKLE E AALR+ + K K+ +
Sbjct: 37 LSKASGQVKRANAAVRKLEGEKAALRERDGGC*ATCYGNCHKLSRGFKERKEDPNEVSVM 216
Query: 664 ENQKSLLQEELMTEKNKLAHISKESKQAEVQAEQFEAKRRQAAKKTEELLSMVSSI 719
+ L+Q EL+ EK K+A + E +QA +Q EQFE K +LLS VS +
Sbjct: 217 GKTEILVQGELVIEKQKMAQLLPELEQARMQQEQFEV----CYFKFIQLLSHVSLV 372
>TC14881
Length = 609
Score = 50.1 bits (118), Expect = 2e-06
Identities = 20/54 (37%), Positives = 31/54 (57%)
Frame = +3
Query: 823 ERECVMCLSEEMSVVFLPCAHQVVCTKCNELHEKQGMQDCPSCRSPIQERISVR 876
++ECV+C++E LPC H +C+ C + Q CP CR PI+E I ++
Sbjct: 90 QKECVICMTEPKDTAVLPCRHMCMCSDCAKELRLQS-NKCPICRQPIEELIEIK 248
>TC15471
Length = 642
Score = 48.1 bits (113), Expect = 6e-06
Identities = 19/53 (35%), Positives = 30/53 (55%)
Frame = +3
Query: 824 RECVMCLSEEMSVVFLPCAHQVVCTKCNELHEKQGMQDCPSCRSPIQERISVR 876
+ECV+CLSE LPC H +C+ C ++ Q CP CR P++ + ++
Sbjct: 168 KECVICLSEPRDTTVLPCRHMCMCSGCAKVLRFQ-TNRCPICRQPVERLLEIK 323
>AV779203
Length = 487
Score = 47.4 bits (111), Expect = 1e-05
Identities = 22/50 (44%), Positives = 30/50 (60%)
Frame = -1
Query: 826 CVMCLSEEMSVVFLPCAHQVVCTKCNELHEKQGMQDCPSCRSPIQERISV 875
C +C E+SVV LPC H+V+C+ C+E +K CP R I ER+ V
Sbjct: 379 CRICFEGEISVVLLPCRHRVLCSTCSEKCKK-----CPISRDSISERVPV 245
>AV766525
Length = 564
Score = 44.7 bits (104), Expect = 7e-05
Identities = 22/55 (40%), Positives = 30/55 (54%), Gaps = 4/55 (7%)
Frame = -1
Query: 821 KRERE----CVMCLSEEMSVVFLPCAHQVVCTKCNELHEKQGMQDCPSCRSPIQE 871
KRER CV+CL +E + VF+PC H CT C+ + CP CR I++
Sbjct: 441 KRERLIPDLCVICLEKEYNSVFVPCGHMCCCTGCS-----SHLTSCPLCRRQIEK 292
>TC8961 similar to GB|BAB71852.1|16902294|AB062739 kinesin-related protein
{Arabidopsis thaliana;} , partial (8%)
Length = 735
Score = 42.4 bits (98), Expect = 3e-04
Identities = 20/80 (25%), Positives = 34/80 (42%), Gaps = 2/80 (2%)
Frame = +1
Query: 796 EEPHTASISELVQKLNNFSMN--GGGVKRERECVMCLSEEMSVVFLPCAHQVVCTKCNEL 853
EEP + +Q++ + G G C +C + + LPC H +C C+
Sbjct: 157 EEPLVVRLKARMQEMKEKELKHLGNGDANSHVCKVCFESSTAAILLPCRHFCLCKSCS-- 330
Query: 854 HEKQGMQDCPSCRSPIQERI 873
+CP CR+ I +R+
Sbjct: 331 ---LACSECPICRTSIADRL 381
>BP030839
Length = 379
Score = 40.8 bits (94), Expect = 0.001
Identities = 19/47 (40%), Positives = 23/47 (48%)
Frame = -3
Query: 821 KRERECVMCLSEEMSVVFLPCAHQVVCTKCNELHEKQGMQDCPSCRS 867
+R R C C EE V+ LPC H VCT C + CP C+S
Sbjct: 176 RRRRLCRKCGKEESCVLILPCRHLCVCTLCGST-----LHHCPICKS 51
>TC17849
Length = 589
Score = 40.8 bits (94), Expect = 0.001
Identities = 29/109 (26%), Positives = 48/109 (43%)
Frame = +2
Query: 767 DSSKIAALKRGIDGSYAGSFKDTKKGSGFEEPHTASISELVQKLNNFSMNGGGVKREREC 826
D K A L+R ID + K+++ +E+ +K+ + K C
Sbjct: 26 DVEKKALLQRTID--LQENLKESQPAFLYEQ----------EKVERATKEADTAKAAWVC 169
Query: 827 VMCLSEEMSVVFLPCAHQVVCTKCNELHEKQGMQDCPSCRSPIQERISV 875
+CLS E+ + +PC H V+C KC+ K CP CR + + I +
Sbjct: 170 RVCLSAEVDITIVPCGH-VLCRKCSSAVSK-----CPFCRLQVTKAIRI 298
>TC9015 similar to UP|Q9SS90 (Q9SS90) F4P13.19 protein, partial (12%)
Length = 639
Score = 38.9 bits (89), Expect = 0.004
Identities = 20/50 (40%), Positives = 26/50 (52%)
Frame = +1
Query: 826 CVMCLSEEMSVVFLPCAHQVVCTKCNELHEKQGMQDCPSCRSPIQERISV 875
C +CL+ + F C HQ C +C Q +Q CP CRSPI RI +
Sbjct: 211 CPICLTNSKDMAF-GCGHQTCC-ECG-----QDLQSCPICRSPINTRIKL 339
>TC11541 weakly similar to UP|O64756 (O64756) At2g34920 protein, partial
(6%)
Length = 553
Score = 38.9 bits (89), Expect = 0.004
Identities = 24/55 (43%), Positives = 31/55 (55%), Gaps = 1/55 (1%)
Frame = +1
Query: 818 GGVKRERECVMCLSEEMSVVFLPCAHQVVCTKC-NELHEKQGMQDCPSCRSPIQE 871
G VK+ CV C S S+++ C H C+KC NEL +G CP CR+PI E
Sbjct: 154 GHVKKGTCCVCCDSHIDSLLYR-CGHMCTCSKCANEL--IRGGGKCPLCRAPIVE 309
>TC10929 similar to UP|Q94AX6 (Q94AX6) AT3g23280/K14B15_17, partial (16%)
Length = 783
Score = 38.5 bits (88), Expect = 0.005
Identities = 24/76 (31%), Positives = 35/76 (45%), Gaps = 1/76 (1%)
Frame = +2
Query: 801 ASISELVQKLNNFSMNGGGVKRERECVMCLSEEMSVVFLPCAHQVVCTKC-NELHEKQGM 859
+S S V+KL + G + CV+CL +PC H C C NE+ K+
Sbjct: 200 SSSSSAVEKLPEEGQSAGEIGST--CVICLDAPAEGACIPCGHVAGCMSCLNEVKTKK-- 367
Query: 860 QDCPSCRSPIQERISV 875
CP CR+ I + I +
Sbjct: 368 WGCPVCRAKIDQVIKL 415
>TC13326
Length = 578
Score = 38.1 bits (87), Expect = 0.006
Identities = 35/149 (23%), Positives = 57/149 (37%), Gaps = 1/149 (0%)
Frame = +2
Query: 721 KEREQIEELARTKEERIKLEAEKELRRYKDDIQKLEKEIAQIRQKSDSSKIAALKRGIDG 780
K++ ++EE I+LEAE +L R E E + + ++ RG+
Sbjct: 8 KKKTELEEFL------IRLEAENQLWRRVAQ----ENEAVVLSLHNTLEQVKERNRGLVA 157
Query: 781 SYAGSFK-DTKKGSGFEEPHTASISELVQKLNNFSMNGGGVKRERECVMCLSEEMSVVFL 839
A S+ D +G+ + T E+ ++ + C C S +FL
Sbjct: 158 EDAESYCCDENRGNRVMQEGTGETEEIGKETTE--------QITMVCKCCHSRSSCFMFL 313
Query: 840 PCAHQVVCTKCNELHEKQGMQDCPSCRSP 868
PC H C C +Q CP C+ P
Sbjct: 314 PCRHLCSCKACEPF-----LQACPVCKMP 385
>BP077155
Length = 432
Score = 37.4 bits (85), Expect = 0.010
Identities = 18/42 (42%), Positives = 21/42 (49%)
Frame = -2
Query: 824 RECVMCLSEEMSVVFLPCAHQVVCTKCNELHEKQGMQDCPSC 865
REC C SVV LPC H VCT+C+ + CP C
Sbjct: 281 RECKGCWKRVASVVVLPCRHLCVCTECD-----AQFRACPVC 171
>CB826758
Length = 537
Score = 37.0 bits (84), Expect = 0.014
Identities = 37/143 (25%), Positives = 65/143 (44%), Gaps = 6/143 (4%)
Frame = +2
Query: 622 EMENAALRKEMEAAKLRAVESATNFQEVSKREKKTQMKFQSWENQKSLLQEELMTEKNKL 681
E ENA E + E + E +R K + + N + + +EEL TEK +L
Sbjct: 41 EAENATRHAYQETFRRGRAEK--DAIEAIRRAKAAESLYAEELNLRKMAEEELETEKEEL 214
Query: 682 AHISKESKQAEVQAEQFEAKRRQAAKKTEELLSMVSSIRKEREQ-----IEELARTKEER 736
++ QA+ E+ + Q + +L S + KE EQ ++ L K ER
Sbjct: 215 ENL---KSQADKVKEELRLVQDQKSSLESQLASSELMV-KELEQKIISAVDLLQNYKNER 382
Query: 737 IKLEAEKE-LRRYKDDIQKLEKE 758
+LE +++ L R ++++K + E
Sbjct: 383 EELEVQRDSLLREAEELRKKQGE 451
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.310 0.125 0.342
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,582,062
Number of Sequences: 28460
Number of extensions: 188130
Number of successful extensions: 1176
Number of sequences better than 10.0: 103
Number of HSP's better than 10.0 without gapping: 1131
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1161
length of query: 880
length of database: 4,897,600
effective HSP length: 98
effective length of query: 782
effective length of database: 2,108,520
effective search space: 1648862640
effective search space used: 1648862640
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 59 (27.3 bits)
Medicago: description of AC146553.15