BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146553.13 + phase: 0
(198 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV774105 43 1e-10
TC17632 similar to GB|AAO44044.1|28466871|BT004778 At4g24310 {Ar... 57 2e-09
AV765222 31 0.16
TC9068 similar to UP|SCWA_YEAST (Q04951) Probable family 17 gluc... 28 1.4
AV416787 27 2.4
TC15382 similar to UP|MSK2_MEDSA (P51138) Glycogen synthase kina... 27 3.1
TC16179 weakly similar to UP|Q7PXR0 (Q7PXR0) AgCP12009, partial ... 26 4.0
TC10770 weakly similar to UP|RN12_HUMAN (Q9NVW2) RING finger pro... 26 4.0
AV776116 26 5.2
AW720511 25 6.8
TC15670 25 6.8
TC12489 25 6.8
BP080441 25 6.8
>AV774105
Length = 487
Score = 43.1 bits (100), Expect(2) = 1e-10
Identities = 20/54 (37%), Positives = 30/54 (55%), Gaps = 1/54 (1%)
Frame = +3
Query: 54 HCS-TINKYLSGILLVICGFNCAFTSFTDSYTGSDGQRHYGIVTMNGLWPSPGS 106
HC ++ K+++ LL +CG +C FTDS+ S G YG+ T G+ GS
Sbjct: 309 HCDDSVTKFMTAALLSLCGASCFLQCFTDSFRDSKGSVCYGLATFRGVRVIDGS 470
Score = 37.7 bits (86), Expect(2) = 1e-10
Identities = 16/25 (64%), Positives = 19/25 (76%)
Frame = +2
Query: 29 NLVKLLPTGTVFLFQYLSPVVTNNG 53
+L LLPTGTV FQ+LSP+ TN G
Sbjct: 233 HLANLLPTGTVLSFQFLSPIFTNRG 307
>TC17632 similar to GB|AAO44044.1|28466871|BT004778 At4g24310 {Arabidopsis
thaliana;}, partial (31%)
Length = 396
Score = 57.4 bits (137), Expect = 2e-09
Identities = 26/67 (38%), Positives = 39/67 (57%)
Frame = +1
Query: 16 ASTMTGKTFSGVGNLVKLLPTGTVFLFQYLSPVVTNNGHCSTINKYLSGILLVICGFNCA 75
A +T + L LLPTGT+ FQ L+PV TNNG C ++ + L+ +LL++ G +
Sbjct: 187 ARRAVSQTLTSTATLANLLPTGTLLAFQMLTPVFTNNGSCDSVTRLLTLMLLLLLGLSSF 366
Query: 76 FTSFTDS 82
FTD+
Sbjct: 367 LACFTDT 387
>AV765222
Length = 324
Score = 30.8 bits (68), Expect = 0.16
Identities = 11/16 (68%), Positives = 14/16 (86%)
Frame = -1
Query: 172 FMIFPSYRHGIGYPTS 187
F++FP+ RHGIGYP S
Sbjct: 324 FVVFPTTRHGIGYPRS 277
>TC9068 similar to UP|SCWA_YEAST (Q04951) Probable family 17 glucosidase
SCW10 precursor (Soluble cell wall protein 10) ,
partial (6%)
Length = 1209
Score = 27.7 bits (60), Expect = 1.4
Identities = 13/40 (32%), Positives = 24/40 (59%)
Frame = -1
Query: 2 SEKASSSKTSTNKGASTMTGKTFSGVGNLVKLLPTGTVFL 41
S +SSS +S++ +S+ + + SG+G +L P G V +
Sbjct: 153 SSSSSSSSSSSSSSSSSSSSSSSSGLGLNKRLKPDGAVLI 34
>AV416787
Length = 413
Score = 26.9 bits (58), Expect = 2.4
Identities = 13/36 (36%), Positives = 21/36 (58%)
Frame = +3
Query: 116 LRFGDFVHAFLSVIVFAVLGLLDTNVVHCFYPKFES 151
L F ++++AF+ G+L T +VHC Y F+S
Sbjct: 192 LHFQEYLNAFV--------GILGTALVHCLYFNFQS 275
>TC15382 similar to UP|MSK2_MEDSA (P51138) Glycogen synthase kinase-3
homolog MsK-2 , partial (17%)
Length = 552
Score = 26.6 bits (57), Expect = 3.1
Identities = 14/54 (25%), Positives = 25/54 (45%)
Frame = -2
Query: 2 SEKASSSKTSTNKGASTMTGKTFSGVGNLVKLLPTGTVFLFQYLSPVVTNNGHC 55
+ K ++ K KG + +TGKT + + + + G ++ L NN HC
Sbjct: 350 TRKITNWKCDGRKGTNKLTGKTMNILTD*IYFYRQGVDNIYTRLLHYEPNNAHC 189
>TC16179 weakly similar to UP|Q7PXR0 (Q7PXR0) AgCP12009, partial (20%)
Length = 814
Score = 26.2 bits (56), Expect = 4.0
Identities = 16/42 (38%), Positives = 23/42 (54%)
Frame = +2
Query: 94 IVTMNGLWPSPGSDSVDLSAYKLRFGDFVHAFLSVIVFAVLG 135
++ NG++PSP +S S Y L G +F+ V V VLG
Sbjct: 578 LI*FNGVFPSPAVESDY*STYSL--GQMFCSFVVVSVLIVLG 697
>TC10770 weakly similar to UP|RN12_HUMAN (Q9NVW2) RING finger protein 12
(LIM domain interacting RING finger protein) (RING
finger LIM domain-binding protein) (R-LIM) (NY-REN-43
antigen), partial (4%)
Length = 481
Score = 26.2 bits (56), Expect = 4.0
Identities = 21/80 (26%), Positives = 33/80 (41%)
Frame = -2
Query: 1 MSEKASSSKTSTNKGASTMTGKTFSGVGNLVKLLPTGTVFLFQYLSPVVTNNGHCSTINK 60
MS +SSS S+ +S+ G S ++ + G LF+Y + +G S K
Sbjct: 282 MSSSSSSSSPSSRSASSSSRGTEGSSSPSVTDAISDGWYSLFRYSDMLNLRSGFSSRSFK 103
Query: 61 YLSGILLVICGFNCAFTSFT 80
S ++ G A S T
Sbjct: 102 NCSYSECILSGSALATLSST 43
>AV776116
Length = 542
Score = 25.8 bits (55), Expect = 5.2
Identities = 12/39 (30%), Positives = 20/39 (50%), Gaps = 1/39 (2%)
Frame = -2
Query: 143 HCFYPKFESSEKILMQVLPPV-IGVVSGAVFMIFPSYRH 180
HC+ S I P + +G + A+++IFP +RH
Sbjct: 223 HCYLSSLSSHRSI-----PGITVGRIPAALYIIFPQFRH 122
>AW720511
Length = 477
Score = 25.4 bits (54), Expect = 6.8
Identities = 12/29 (41%), Positives = 19/29 (65%), Gaps = 1/29 (3%)
Frame = -3
Query: 91 HYGIVTMNGLW-PSPGSDSVDLSAYKLRF 118
HYG+VTM G++ P+ ++ L A L+F
Sbjct: 454 HYGMVTMPGVFTPTEAFQAIRLGASALKF 368
>TC15670
Length = 582
Score = 25.4 bits (54), Expect = 6.8
Identities = 12/28 (42%), Positives = 18/28 (63%)
Frame = +3
Query: 103 SPGSDSVDLSAYKLRFGDFVHAFLSVIV 130
SP + VDLS +LR G F+H + +I+
Sbjct: 222 SPVQNFVDLSGAELRVGVFMHVWTFLIL 305
>TC12489
Length = 646
Score = 25.4 bits (54), Expect = 6.8
Identities = 11/27 (40%), Positives = 16/27 (58%)
Frame = +3
Query: 54 HCSTINKYLSGILLVICGFNCAFTSFT 80
H ST +SG+L+V CG ++ FT
Sbjct: 81 HSSTFLHCVSGVLVVWCGVVWCYSQFT 161
>BP080441
Length = 382
Score = 25.4 bits (54), Expect = 6.8
Identities = 9/22 (40%), Positives = 15/22 (67%)
Frame = -1
Query: 41 LFQYLSPVVTNNGHCSTINKYL 62
+F +L PV+ C+TI+K+L
Sbjct: 76 IFFFLKPVIVGQRPCATIHKFL 11
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.320 0.136 0.404
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,391,000
Number of Sequences: 28460
Number of extensions: 47155
Number of successful extensions: 226
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 226
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 226
length of query: 198
length of database: 4,897,600
effective HSP length: 86
effective length of query: 112
effective length of database: 2,450,040
effective search space: 274404480
effective search space used: 274404480
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 53 (25.0 bits)
Medicago: description of AC146553.13