BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146552.9 - phase: 0 /pseudo
(454 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV409734 170 5e-43
TC16178 similar to UP|Q944A6 (Q944A6) At1g09020/F7G19_11, partia... 124 3e-29
TC12841 similar to UP|Q84PE0 (Q84PE0) AKIN betagamma, partial (19%) 119 1e-27
AV416787 99 2e-21
AV407775 97 5e-21
BP036052 43 9e-05
TC16796 similar to UP|Q7XYX7 (Q7XYX7) AKIN beta2, partial (55%) 37 0.005
TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partia... 33 0.13
TC14910 similar to UP|Q84PE0 (Q84PE0) AKIN betagamma, partial (9%) 30 0.63
TC9591 similar to UP|Q93X44 (Q93X44) Protein tyrosine phosphatas... 28 4.1
TC8532 homologue to UP|COMT_MEDSA (P28002) Caffeic acid 3-O-meth... 27 5.3
AV775659 27 9.1
>AV409734
Length = 361
Score = 170 bits (430), Expect = 5e-43
Identities = 86/101 (85%), Positives = 88/101 (86%)
Frame = +2
Query: 223 LILRELGNHGSNLTEEELETHTISAWKEGKWTLFSRRFIHAGPSDNLKDVALKILQNGIS 282
LILRELGNHGSNLTEEELETHTISAWKEGKWT FSRRFIHAGP DNL DVA+KILQN IS
Sbjct: 2 LILRELGNHGSNLTEEELETHTISAWKEGKWTAFSRRFIHAGPYDNLNDVAVKILQNEIS 181
Query: 283 TVPIIHSSSADGSFPQLLHLASLSGILRCILGVVLVHCPYF 323
TVPIIHSSS DGSFPQLLHLASLSGIL+CI C YF
Sbjct: 182 TVPIIHSSSEDGSFPQLLHLASLSGILKCI-------CRYF 283
Score = 62.4 bits (150), Expect = 1e-10
Identities = 26/40 (65%), Positives = 29/40 (72%)
Frame = +3
Query: 299 LLHLASLSGILRCILGVVLVHCPYFNFQFVQFLWAHGCPK 338
LLH + ILG+VL HC YFNFQ+VQFLWAHGCPK
Sbjct: 240 LLHFRGSLNVYAGILGIVLAHCLYFNFQYVQFLWAHGCPK 359
>TC16178 similar to UP|Q944A6 (Q944A6) At1g09020/F7G19_11, partial (19%)
Length = 673
Score = 124 bits (311), Expect = 3e-29
Identities = 59/68 (86%), Positives = 64/68 (93%)
Frame = +3
Query: 387 LDIYCRSDITALAKDRAYTHINLDEMTVHQALQLSQDAFNPNESRSQRCQMCLRTDSLHK 446
LDIYCRSDITALAKDRAY+HINLDEMTVH+ALQL QDA++P E RSQRCQMCLR+DSL K
Sbjct: 3 LDIYCRSDITALAKDRAYSHINLDEMTVHEALQLGQDAYSPYELRSQRCQMCLRSDSLQK 182
Query: 447 VMERLANP 454
VMERLANP
Sbjct: 183 VMERLANP 206
>TC12841 similar to UP|Q84PE0 (Q84PE0) AKIN betagamma, partial (19%)
Length = 279
Score = 119 bits (297), Expect = 1e-27
Identities = 61/94 (64%), Positives = 72/94 (75%), Gaps = 12/94 (12%)
Frame = +1
Query: 202 APLWDFCKGQFVGVLSVLDFILILRELGNHGSNLTEEELETHTISAWKEGKWTLFSRR-- 259
APLWDF + +FVGVLS +DFILIL+ELGNHGS LTEE+LETHTI+AWKEGK L RR
Sbjct: 4 APLWDFGRSKFVGVLSAMDFILILKELGNHGSKLTEEQLETHTIAAWKEGK--LQQRRAV 177
Query: 260 ----------FIHAGPSDNLKDVALKILQNGIST 283
F+HAGP ++LKDVALK+LQN +ST
Sbjct: 178 DSNGGSYPSLFVHAGPHEHLKDVALKVLQNKVST 279
>AV416787
Length = 413
Score = 98.6 bits (244), Expect = 2e-21
Identities = 63/128 (49%), Positives = 75/128 (58%), Gaps = 5/128 (3%)
Frame = +2
Query: 257 SRRFIHAGPSDNLKDVALKILQNGISTVPIIHSSSADGSFPQLLHLASLSGILRCILGVV 316
SR FIHAGP DNLKD+A+KILQ +STVPI+HSSS DGS+PQLLHLASLSGIL+CI
Sbjct: 53 SRLFIHAGPYDNLKDIAMKILQKEVSTVPIMHSSSEDGSYPQLLHLASLSGILKCICR-Y 229
Query: 317 LVHC----PYFNFQFVQFLWAHGCPKLGRQIAGL*QH*DQMLLLLQP-*IY*FKFNIFFA 371
HC P F PK+G + L +L+P N+
Sbjct: 230 FRHCSSSLPILQFPICAIPVGTWVPKIGESSR-------RPLAMLRPSSSLAAALNLLVQ 388
Query: 372 AQVSSIPI 379
A+VSSIPI
Sbjct: 389 AEVSSIPI 412
>AV407775
Length = 426
Score = 97.1 bits (240), Expect = 5e-21
Identities = 47/62 (75%), Positives = 51/62 (81%)
Frame = +1
Query: 1 MFSPSMDSARDVGGVVAAGTVLIPVRFVWPYGGRTVYLSGSFTRWSELLQMSPVEGCPTV 60
MFS SMD AR GV GTVLIP+RFVWPYGGR+V+L+GSFT WSE L MSPVEGCPTV
Sbjct: 247 MFSQSMDPARGASGV--GGTVLIPMRFVWPYGGRSVFLTGSFTGWSEPLPMSPVEGCPTV 420
Query: 61 FQ 62
FQ
Sbjct: 421 FQ 426
>BP036052
Length = 389
Score = 43.1 bits (100), Expect = 9e-05
Identities = 27/80 (33%), Positives = 39/80 (48%), Gaps = 1/80 (1%)
Frame = -1
Query: 25 VRFVWPYGGRTVYLSGSFTRWSELLQMSP-VEGCPTVFQVIHNLAPGYHQYKFFVDGEWR 83
V W +V + G+F WS+ +SP G T F L PG ++ KF VDGEW+
Sbjct: 317 VSVFWVGMAESVQVMGTFDGWSQGEHLSPEYTGSYTRFSTTLMLRPGRYEIKFLVDGEWK 138
Query: 84 HDEHTPHITGDYGIVNTVLL 103
P +TG+ N +L+
Sbjct: 137 LSPEFP-LTGEGLTKNNLLI 81
>TC16796 similar to UP|Q7XYX7 (Q7XYX7) AKIN beta2, partial (55%)
Length = 746
Score = 37.4 bits (85), Expect = 0.005
Identities = 26/104 (25%), Positives = 44/104 (42%), Gaps = 10/104 (9%)
Frame = +2
Query: 61 FQVIHNLAPGYHQYKFFVDGEWRHDEHTPHITGDYGIVNTVLLATD----------PFVP 110
F ++ L G +QY+F VDG+WR+ P D G +L + F P
Sbjct: 26 FTIMKVLPSGVYQYRFVVDGQWRYAPELPWAQDDAGNAYNILDLQEYVPEDIGGISSFEP 205
Query: 111 VLPPDIVSGSNMDVDNETFQRVVRLTDGTLSEVMPRISDVDVQT 154
PD S SN+ + +E + + L L + + +++T
Sbjct: 206 PKSPD-SSYSNLQLGSEDYAKEPPLVPPFLQMTLLNVPSANMET 334
>TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partial (20%)
Length = 739
Score = 32.7 bits (73), Expect = 0.13
Identities = 18/48 (37%), Positives = 25/48 (51%)
Frame = +3
Query: 56 GCPTVFQVIHNLAPGYHQYKFFVDGEWRHDEHTPHITGDYGIVNTVLL 103
G T F L PG ++ KF VDGEW+ P +TG+ N +L+
Sbjct: 21 GSYTRFSTTLMLRPGRYEIKFLVDGEWKLSPEFP-LTGEGLTKNNLLI 161
>TC14910 similar to UP|Q84PE0 (Q84PE0) AKIN betagamma, partial (9%)
Length = 716
Score = 30.4 bits (67), Expect = 0.63
Identities = 13/14 (92%), Positives = 13/14 (92%)
Frame = +1
Query: 441 TDSLHKVMERLANP 454
TD LHKVMERLANP
Sbjct: 1 TDPLHKVMERLANP 42
>TC9591 similar to UP|Q93X44 (Q93X44) Protein tyrosine phosphatase, partial
(36%)
Length = 638
Score = 27.7 bits (60), Expect = 4.1
Identities = 17/60 (28%), Positives = 29/60 (48%), Gaps = 1/60 (1%)
Frame = +2
Query: 24 PVRFVWPYGGRT-VYLSGSFTRWSELLQMSPVEGCPTVFQVIHNLAPGYHQYKFFVDGEW 82
PV W + + V +SG W + + + + + F + L G ++YK+ VDGEW
Sbjct: 83 PVTLSWEHRNCSRVEISGLDIGWGQRMPLHFDDKRGSWF-LYRELPEGRYEYKYIVDGEW 259
>TC8532 homologue to UP|COMT_MEDSA (P28002) Caffeic acid
3-O-methyltransferase (S-adenosysl-L-methionine:caffeic
acid 3-O-methyltransferase) (COMT) (CAOMT) , partial
(36%)
Length = 688
Score = 27.3 bits (59), Expect = 5.3
Identities = 10/23 (43%), Positives = 16/23 (69%)
Frame = +1
Query: 165 MRTAYELLPESGKVVTLDVDLPV 187
++ YE LP++GKV+ + LPV
Sbjct: 139 LKNCYEALPDNGKVIVAECILPV 207
>AV775659
Length = 439
Score = 26.6 bits (57), Expect = 9.1
Identities = 19/75 (25%), Positives = 28/75 (37%)
Frame = +3
Query: 216 LSVLDFILILRELGNHGSNLTEEELETHTISAWKEGKWTLFSRRFIHAGPSDNLKDVALK 275
+S+ F L + E +HG L + + G+W + F GP +
Sbjct: 216 ISLTTFSLSIEETSSHGLTLHSNSPNQFPSAIYTNGEWLIL---FHP*GPRPKI*GTGCL 386
Query: 276 ILQNGISTVPIIHSS 290
QN IS P H S
Sbjct: 387 QCQNQISYPPQFHDS 431
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.328 0.142 0.446
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,249,212
Number of Sequences: 28460
Number of extensions: 121987
Number of successful extensions: 725
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 721
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 722
length of query: 454
length of database: 4,897,600
effective HSP length: 93
effective length of query: 361
effective length of database: 2,250,820
effective search space: 812546020
effective search space used: 812546020
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 57 (26.6 bits)
Medicago: description of AC146552.9