BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146552.7 + phase: 0 /pseudo
(720 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV779136 298 2e-81
TC18983 homologue to UP|Q8LP18 (Q8LP18) Cullin-like protein1, pa... 183 1e-46
AV425591 142 1e-34
AW719682 115 2e-26
TC19035 UP|Q84LL5 (Q84LL5) Cullin 4, partial (14%) 99 2e-21
TC10578 similar to UP|CAC87839 (CAC87839) Cullin 3B (Fragment), ... 86 3e-17
AV406481 79 2e-15
TC9828 similar to UP|Q7Y1Y8 (Q7Y1Y8) Cullin 3a (Fragment), parti... 64 6e-11
TC15368 homologue to UP|Q84LL5 (Q84LL5) Cullin 4, partial (11%) 49 4e-06
AW428720 34 0.071
TC10686 homologue to UP|Q8LP18 (Q8LP18) Cullin-like protein1, pa... 32 0.27
AV422604 31 0.61
BF177505 30 1.0
TC16670 similar to PIR|JC7519|JC7519 subtilisin-like serine prot... 29 2.3
TC18160 homologue to UP|Q9ZP40 (Q9ZP40) Plastoglobule associated... 28 5.1
TC8018 27 8.7
>AV779136
Length = 513
Score = 298 bits (764), Expect = 2e-81
Identities = 150/166 (90%), Positives = 158/166 (94%)
Frame = -1
Query: 550 RKLTWIYSLGTCNISGKFDPKTVELVVTTYQASALLLFNSSDRLSYSEIMTQLNLLDEDV 609
RKLTWIYSLGTCN+ GKFDPKTVELVVTTYQASALLL NSSDRLSYSEIMTQLNL D+DV
Sbjct: 513 RKLTWIYSLGTCNVVGKFDPKTVELVVTTYQASALLLCNSSDRLSYSEIMTQLNLTDDDV 334
Query: 610 IRLLHSLSCAKYKILIKEPNTKTILPTDYFEFNAKFTDKMRRIKIPLPPVDEKKKVIEDV 669
IRLLHSLSCAKYKIL +EPNT TI TDYFE N+KFTDK+RRIKIPLPPVDEKKKVIE+V
Sbjct: 333 IRLLHSLSCAKYKILNQEPNTNTISNTDYFECNSKFTDKLRRIKIPLPPVDEKKKVIENV 154
Query: 670 DKDRRYAIDASIVRIMKSRKVLGYQQLVMECVEQLGRMFKPDVKAI 715
DKDRRYAIDAS+VRIMKSRKVLGY+QLVMECVEQLGRMFKPDVKAI
Sbjct: 153 DKDRRYAIDASLVRIMKSRKVLGYRQLVMECVEQLGRMFKPDVKAI 16
>TC18983 homologue to UP|Q8LP18 (Q8LP18) Cullin-like protein1, partial (13%)
Length = 425
Score = 183 bits (464), Expect = 1e-46
Identities = 85/95 (89%), Positives = 91/95 (95%)
Frame = +2
Query: 1 MSERKTIDLEQGWDFMHRGIMKLKNILEGLPEPQFSPEDYMMLYTTIYNMCTQKPPNDYS 60
MSERKTIDLEQGWDFM +GI KLKNILEGLPEPQFS EDYMMLYTTIYNMCTQKPP+DYS
Sbjct: 140 MSERKTIDLEQGWDFMQKGITKLKNILEGLPEPQFSSEDYMMLYTTIYNMCTQKPPHDYS 319
Query: 61 QPLYDKYKEAFEEYIVSTVLPSLREKHDEFMLREL 95
Q LYDKY+E+FEEYI+STVLP+LREKHDEFMLREL
Sbjct: 320 QQLYDKYRESFEEYIISTVLPALREKHDEFMLREL 424
>AV425591
Length = 247
Score = 142 bits (359), Expect = 1e-34
Identities = 70/80 (87%), Positives = 76/80 (94%)
Frame = +3
Query: 419 KVVKLLAYISDKDLFAEFYRKKLARRLLFDKSANDDHERSILTKLKQQCGGQFTSKMEGM 478
+VVKLLAYI DKDLFAEFYRKKLARRLLFDKSANDDHERSIL+KLKQQCG QFT KMEGM
Sbjct: 3 EVVKLLAYIGDKDLFAEFYRKKLARRLLFDKSANDDHERSILSKLKQQCGQQFTVKMEGM 182
Query: 479 VTDLTLAKENQTSFEEYLSN 498
V DLT+AKENQT+F+EYLS+
Sbjct: 183 VNDLTMAKENQTNFDEYLSD 242
>AW719682
Length = 480
Score = 115 bits (288), Expect = 2e-26
Identities = 60/137 (43%), Positives = 90/137 (64%)
Frame = +1
Query: 342 FVRKVIELHDKYLAYVNSCFQNHTLFHKALKEAFEVFCNKGVAGNSSAELLATFCDNILK 401
FV++++ DKY +N F N LF AL +FE F N S E ++ F D+ L+
Sbjct: 82 FVQRLLHEKDKYDKIINLAFNNDKLFQNALSSSFEYFIN---LNPRSPEFISLFVDDKLR 252
Query: 402 KGGSEKLSDEAIEETLEKVVKLLAYISDKDLFAEFYRKKLARRLLFDKSANDDHERSILT 461
KG + +S++ IE TL+KV+ L Y+ +KD+F ++Y++ LA+RLL K+ +DD ERS++
Sbjct: 253 KG-LKGVSEDDIETTLDKVMMLFRYLQEKDVFEKYYKQHLAKRLLSGKTVSDDAERSLIV 429
Query: 462 KLKQQCGGQFTSKMEGM 478
KLK +CG QFTSK+EGM
Sbjct: 430 KLKTECGYQFTSKLEGM 480
>TC19035 UP|Q84LL5 (Q84LL5) Cullin 4, partial (14%)
Length = 305
Score = 99.0 bits (245), Expect = 2e-21
Identities = 47/101 (46%), Positives = 69/101 (67%)
Frame = +2
Query: 442 ARRLLFDKSANDDHERSILTKLKQQCGGQFTSKMEGMVTDLTLAKENQTSFEEYLSNTPN 501
A+RLL KSA+ D E+S+++KLK +CG QFT+K+EGM D+ L+KE SF++
Sbjct: 2 AKRLLLGKSASIDAEKSMISKLKTECGSQFTNKLEGMFKDIELSKEINESFKQSSQARTK 181
Query: 502 ADPGIDLTVTVLTTGFWPSYKSFDLNLPAEMVKCVEVFKEF 542
GI+++V VLTTG+WP+Y D+ LP E+ ++FKEF
Sbjct: 182 LPSGIEMSVHVLTTGYWPTYPPMDVRLPHELNVYQDIFKEF 304
>TC10578 similar to UP|CAC87839 (CAC87839) Cullin 3B (Fragment), partial
(29%)
Length = 590
Score = 85.5 bits (210), Expect = 3e-17
Identities = 56/140 (40%), Positives = 79/140 (56%), Gaps = 6/140 (4%)
Frame = +3
Query: 587 FNSSDRLSYSEIMTQLNLLDEDVIRLLHSLSCAKYK-ILIKEPNTKTILPTDYFEFNAKF 645
FN++DRL+ EI + D+ R L SL+C K K +L KEP +K I D F FN +F
Sbjct: 42 FNNADRLTCKEIEQATAIPMSDLKRCLQSLACVKGKNVLRKEPMSKDIAEDDAFLFNDQF 221
Query: 646 TDKMRRIKIPLPPVD-----EKKKVIEDVDKDRRYAIDASIVRIMKSRKVLGYQQLVMEC 700
T K ++KI E + + V++DR+ I+A+IVRIMKSR+ L + +V E
Sbjct: 222 TSKFFKVKIGTVVAQRESEPENLETRQRVEEDRKPQIEAAIVRIMKSRRTLDHNHVVAEV 401
Query: 701 VEQLGRMFKPDVKAI*KRIE 720
+QL F P+ I KRIE
Sbjct: 402 TKQLQSRFLPNPVVIKKRIE 461
>AV406481
Length = 239
Score = 79.3 bits (194), Expect = 2e-15
Identities = 37/41 (90%), Positives = 38/41 (92%)
Frame = +3
Query: 1 MSERKTIDLEQGWDFMHRGIMKLKNILEGLPEPQFSPEDYM 41
MSERKTIDLEQGWDFM +GI KLKNILEGLPEPQFS EDYM
Sbjct: 117 MSERKTIDLEQGWDFMLKGITKLKNILEGLPEPQFSSEDYM 239
>TC9828 similar to UP|Q7Y1Y8 (Q7Y1Y8) Cullin 3a (Fragment), partial (39%)
Length = 551
Score = 64.3 bits (155), Expect = 6e-11
Identities = 40/103 (38%), Positives = 58/103 (55%), Gaps = 5/103 (4%)
Frame = +3
Query: 623 ILIKEPNTKTILPTDYFEFNAKFTDKMRRIKI-----PLPPVDEKKKVIEDVDKDRRYAI 677
+L KEP TK + D F N KF+ K+ ++KI P EK + V++DR+ I
Sbjct: 24 VLRKEPMTKDLGEDDAFFVNDKFSSKLYKVKIGTVVAPKESEPEKLETRPRVEEDRKPQI 203
Query: 678 DASIVRIMKSRKVLGYQQLVMECVEQLGRMFKPDVKAI*KRIE 720
+A++VRIMKSRK L + L+ E +QL F + + KRIE
Sbjct: 204 EAALVRIMKSRKQLDHNHLIAEVTKQLQSRFLANPTEVKKRIE 332
>TC15368 homologue to UP|Q84LL5 (Q84LL5) Cullin 4, partial (11%)
Length = 517
Score = 48.5 bits (114), Expect = 4e-06
Identities = 30/63 (47%), Positives = 40/63 (62%), Gaps = 1/63 (1%)
Frame = +1
Query: 659 VDEKKKVIEDVDKDRRYAIDASIVRIMKSRKVLGYQQLVMECVEQLGRMFKP-DVKAI*K 717
V+ E V +DR+Y +DA+IVRIMK+RKVL + L+ E +QL KP D+K K
Sbjct: 13 VEVNTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQLKFPIKPADLK---K 183
Query: 718 RIE 720
RIE
Sbjct: 184 RIE 192
>AW428720
Length = 352
Score = 34.3 bits (77), Expect = 0.071
Identities = 16/38 (42%), Positives = 23/38 (60%)
Frame = +1
Query: 190 ENDFEADMLKDTSAYYSRKASNWILEDSCPDYMLKAEE 227
EN F M+K+ +A+Y+ +ASNWI S D K E+
Sbjct: 46 ENHFIGRMIKEHAAFYNDEASNWIASSSLRDNTPKEEK 159
>TC10686 homologue to UP|Q8LP18 (Q8LP18) Cullin-like protein1, partial (5%)
Length = 656
Score = 32.3 bits (72), Expect = 0.27
Identities = 15/16 (93%), Positives = 15/16 (93%)
Frame = +3
Query: 705 GRMFKPDVKAI*KRIE 720
GRMFKPDVKAI KRIE
Sbjct: 3 GRMFKPDVKAIKKRIE 50
>AV422604
Length = 460
Score = 31.2 bits (69), Expect = 0.61
Identities = 18/72 (25%), Positives = 29/72 (40%), Gaps = 11/72 (15%)
Frame = +1
Query: 180 EIGMGKMDHYENDFEADMLKDTSAYYSR-----------KASNWILEDSCPDYMLKAEEC 228
EI +DHY A + K Y + K + W+ + D+ AE C
Sbjct: 193 EINQAGVDHYNKLINALLAKGVEPYVTMYHWDLPQALEDKYNGWLSPEIIKDFANYAETC 372
Query: 229 LRREKDRVAHYL 240
++ DRV H++
Sbjct: 373 FQKFGDRVKHWI 408
>BF177505
Length = 478
Score = 30.4 bits (67), Expect = 1.0
Identities = 29/112 (25%), Positives = 48/112 (41%), Gaps = 6/112 (5%)
Frame = +2
Query: 82 SLREKHDEFMLRELVKRWANHKIMVRWLSRFFHYLDRYFIARRSLPPLNEVGLACFRDLV 141
S R HD + +E + R+ NH + R + R F + R F ++ G + D V
Sbjct: 5 SARADHDFLIDKENLIRYGNHALTYRIVDRIFSQVARKFTSKVE-------GKMGYEDFV 163
Query: 142 YKELHGKMRDAIISL------IDQEREGEQIDRALLKNVLDIFVEIGMGKMD 187
Y +L + + + SL ID + G L +N L F E + +M+
Sbjct: 164 YFKLSEEDKSSEPSLEFWFNCIDLDGNG-----VLTRNELQFFYEEQLHRME 304
>TC16670 similar to PIR|JC7519|JC7519 subtilisin-like serine proteinase -
Arabidopsis thaliana {Arabidopsis
thaliana;} , partial (42%)
Length = 1183
Score = 29.3 bits (64), Expect = 2.3
Identities = 13/26 (50%), Positives = 16/26 (61%)
Frame = +2
Query: 500 PNADPGIDLTVTVLTTGFWPSYKSFD 525
P + G ++ V VL TG WP KSFD
Sbjct: 509 PASGAGGEVIVGVLDTGVWPESKSFD 586
>TC18160 homologue to UP|Q9ZP40 (Q9ZP40) Plastoglobule associated protein
PG1 precursor, partial (15%)
Length = 458
Score = 28.1 bits (61), Expect = 5.1
Identities = 19/58 (32%), Positives = 33/58 (56%), Gaps = 3/58 (5%)
Frame = -3
Query: 535 CVEVFKEFYSTKTKHRKLTWI---YSLGTCNISGKFDPKTVELVVTTYQASALLLFNS 589
C+E+ +FY K+ KL+++ GT IS + + ++ T +ASALL++NS
Sbjct: 456 CLEITYKFYHLKSSFIKLSYVPQQSGQGTERISNNY---LLNYILIT-EASALLIYNS 295
>TC8018
Length = 592
Score = 27.3 bits (59), Expect = 8.7
Identities = 14/38 (36%), Positives = 19/38 (49%)
Frame = -3
Query: 513 LTTGFWPSYKSFDLNLPAEMVKCVEVFKEFYSTKTKHR 550
+TTG S + D NLP V K+F+ + KHR
Sbjct: 416 ITTGNGDSIDNSDHNLPTTQDYLVNASKKFFPSNGKHR 303
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.321 0.137 0.397
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,298,912
Number of Sequences: 28460
Number of extensions: 146007
Number of successful extensions: 692
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 687
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 691
length of query: 720
length of database: 4,897,600
effective HSP length: 97
effective length of query: 623
effective length of database: 2,136,980
effective search space: 1331338540
effective search space used: 1331338540
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 59 (27.3 bits)
Medicago: description of AC146552.7