BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146552.2 - phase: 0 /pseudo
(392 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC7944 homologue to UP|Q9ASR1 (Q9ASR1) At1g56070/T6H22_13 (Elong... 224 1e-59
BP067424 202 6e-53
AV780663 94 4e-20
AV407536 77 6e-15
TC8093 homologue to UP|EFTU_TOBAC (P41342) Elongation factor Tu,... 73 7e-14
TC8094 homologue to UP|EFTU_PEA (O24310) Elongation factor Tu, c... 72 2e-13
TC9899 homologue to PIR|S35701|S35701 translation elongation fac... 56 9e-09
BP070882 53 8e-08
BP069475 49 1e-06
AV408944 42 2e-04
AV409888 40 9e-04
AV769010 33 0.063
TC10571 homologue to PIR|T00956|T00956 translation initiation fa... 30 0.90
BI419132 28 2.6
TC20102 28 2.6
AV410803 27 7.7
BP070821 26 10.0
AV770351 26 10.0
>TC7944 homologue to UP|Q9ASR1 (Q9ASR1) At1g56070/T6H22_13 (Elongation
factor EF-2), complete
Length = 2971
Score = 224 bits (572), Expect = 1e-59
Identities = 137/369 (37%), Positives = 200/369 (54%), Gaps = 16/369 (4%)
Frame = +1
Query: 11 RKKIRNICILAHVDHGKTTLADQLIAAASGGMVHPKVAGKVRFMDYLDEEQRRAITMKSS 70
+ IRN+ ++AHVDHGK+TL D L+AAA G++ +VAG VR D +E R IT+KS+
Sbjct: 253 KHNIRNMSVIAHVDHGKSTLTDSLVAAA--GIIAQEVAGDVRMTDTRADEAERGITIKST 426
Query: 71 SISLHY----------------NHHTVNLIDSPGHIDFCGEVSTAARLSDGALLLVDAVE 114
ISL+Y N + +NLIDSPGH+DF EV+ A R++DGAL++VD VE
Sbjct: 427 GISLYYEMTDVALKSFKGERMGNEYLINLIDSPGHVDFSSEVTAALRITDGALVVVDCVE 606
Query: 115 GVHIQTHAVLRQCWTEMLEPCLVLNKMDRLITELNLTPLEAYTRLLRIVHEVNGIWSAYN 174
GV +QT VLRQ E ++P L +NKMDR EL + EAY R++ N I + Y
Sbjct: 607 GVCVQTETVLRQALGERIKPVLTVNKMDRCFLELQVDGEEAYQTFSRVIENANVIMATYE 786
Query: 175 SEKYLSDVDALLAGGTAAGGEVMEDYDDVEDKFQPQKGNVVFACALDGWGFGIHEFAEIY 234
D LL G+ M P+KG V F+ L GW F + FA++Y
Sbjct: 787 --------DPLL-------GDCM---------VYPEKGTVAFSAGLHGWAFTLTNFAKMY 894
Query: 235 ASKLGGSASVGALLRALWGPWYYNPKTKMIVGKKGISGSKARPMFVQFVLEPLWQVYQGA 294
ASK G S ++ LWG +++P TK K S + R FVQF EP+ Q+
Sbjct: 895 ASKFGVDES--KMMERLWGENFFDPATKKWTSKNTGSATCKRG-FVQFCYEPIKQIINTC 1065
Query: 295 LGGGKGMVEKVIKSFNLQIQARELQNKDSKVVLQAVMSRWLPLSDAILSMVLKCLPDPVE 354
+ K + +++ + +++ E ++ K +++ VM WLP + A+L M++ LP P
Sbjct: 1066MNDQKDKLWPMLQKLGVTMKSEE-KDLMGKPLMKRVMQTWLPAASALLEMMIFHLPSPST 1242
Query: 355 GQKSRISRL 363
Q+ R+ L
Sbjct: 1243AQRYRVENL 1269
>BP067424
Length = 473
Score = 202 bits (515), Expect = 6e-53
Identities = 101/113 (89%), Positives = 104/113 (91%)
Frame = +1
Query: 2 EESTSDNNDRKKIRNICILAHVDHGKTTLADQLIAAASGGMVHPKVAGKVRFMDYLDEEQ 61
E + N R KIRNICILAHVDHGKTTLADQLIA ASGGMVHPKVAG+VRFMDYLDEEQ
Sbjct: 133 EMEEEEVNSRHKIRNICILAHVDHGKTTLADQLIAVASGGMVHPKVAGRVRFMDYLDEEQ 312
Query: 62 RRAITMKSSSISLHYNHHTVNLIDSPGHIDFCGEVSTAARLSDGALLLVDAVE 114
RRAITMKSSSI+L YNHHTVNLIDSPGHIDFCGEVSTAARLSDGALLLVDAVE
Sbjct: 313 RRAITMKSSSIALRYNHHTVNLIDSPGHIDFCGEVSTAARLSDGALLLVDAVE 471
>AV780663
Length = 568
Score = 94.0 bits (232), Expect = 4e-20
Identities = 50/134 (37%), Positives = 76/134 (56%)
Frame = +1
Query: 10 DRKKIRNICILAHVDHGKTTLADQLIAAASGGMVHPKVAGKVRFMDYLDEEQRRAITMKS 69
D ++RN+ ++AHVDHGKTTL D+++ + H R MD E+ R IT+ S
Sbjct: 154 DPNRLRNVAVIAHVDHGKTTLMDRILRQCGADIPHE------RAMDSNSLERERGITIAS 315
Query: 70 SSISLHYNHHTVNLIDSPGHIDFCGEVSTAARLSDGALLLVDAVEGVHIQTHAVLRQCWT 129
++ + + +N++D+PGH D GEV + +GA+ +VDA EG QT VL +
Sbjct: 316 KVTTVSWKENELNMVDTPGHADLGGEVERVVGMVEGAV*VVDAGEGPLAQTKLVLAKALK 495
Query: 130 EMLEPCLVLNKMDR 143
L P L+ NK+DR
Sbjct: 496 YGLRPLLLKNKVDR 537
>AV407536
Length = 382
Score = 76.6 bits (187), Expect = 6e-15
Identities = 47/128 (36%), Positives = 76/128 (58%), Gaps = 5/128 (3%)
Frame = +2
Query: 21 AHVDHGKTTLADQLIAAASGGMVHP--KVAGKVRF---MDYLDEEQRRAITMKSSSISLH 75
AH+D GKTTL ++++ A G +H +V G+ MD +D E+ + IT++S++
Sbjct: 5 AHIDSGKTTLTERVLFYA--GRIHEIHEVRGRDGVGAKMDSMDLEREKGITIQSAATYCS 178
Query: 76 YNHHTVNLIDSPGHIDFCGEVSTAARLSDGALLLVDAVEGVHIQTHAVLRQCWTEMLEPC 135
+ +N+ID+PGH+DF EV A R+ DGA+L++ +V GV Q+ V RQ +
Sbjct: 179 WKDCKINIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSITVDRQMRRYDVPRL 358
Query: 136 LVLNKMDR 143
+NK+DR
Sbjct: 359 AFINKLDR 382
>TC8093 homologue to UP|EFTU_TOBAC (P41342) Elongation factor Tu,
chloroplast precursor (EF-Tu), partial (58%)
Length = 1146
Score = 73.2 bits (178), Expect = 7e-14
Identities = 73/227 (32%), Positives = 111/227 (48%), Gaps = 15/227 (6%)
Frame = +1
Query: 10 DRKKIR-NICILAHVDHGKTTLADQL-IAAASGGMVHPKVAGKVRFMDYLDEEQRRAITM 67
+RKK NI + HVDHGKTTL L +A AS G PK K +D EE+ R IT+
Sbjct: 337 ERKKPHVNIGTIGHVDHGKTTLTAALTMALASLGNSAPK---KYDEIDAAPEERARGITI 507
Query: 68 KSSSISLHYNHHTVNLIDSPGHIDFCGEVSTAARLSDGALLLVDAVEGVHIQT--HAVLR 125
++++ + +D PGH D+ + T A DGA+L+V +G QT H +L
Sbjct: 508 NTATVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADGPMPQTKEHILLA 687
Query: 126 QCWTEMLEPCLV--LNKMDRLITELNLTPLEAYTRLLRIVHEVNGIWSAYNSEKYLSDVD 183
+ ++ P +V LNK D++ E L +E R L +E G S L ++
Sbjct: 688 K---QVGVPNMVVFLNKQDQVDDEELLQLVELEVRELLSSYEFPGDDVPIVSGSALLALE 858
Query: 184 ALLAGGTAAGGE------VMEDYDDVEDKFQP---QKGNVVFACALD 221
AL+A GE + E D+V D + P ++ ++ F CA++
Sbjct: 859 ALMANPAIKRGENQWVDKIYELMDNV-DSYIPIPQRQTDLPFLCAIE 996
>TC8094 homologue to UP|EFTU_PEA (O24310) Elongation factor Tu, chloroplast
precursor (EF-Tu), partial (60%)
Length = 1092
Score = 71.6 bits (174), Expect = 2e-13
Identities = 70/213 (32%), Positives = 97/213 (44%), Gaps = 8/213 (3%)
Frame = +1
Query: 10 DRKKIR-NICILAHVDHGKTTLADQL-IAAASGGMVHPKVAGKVRFMDYLDEEQRRAITM 67
+RKK NI + HVDHGKTTL L +A AS G PK K +D EE+ R IT+
Sbjct: 310 ERKKPHVNIGTIGHVDHGKTTLTAALTMALASLGNSAPK---KYDEIDAAPEERARGITI 480
Query: 68 KSSSISL-----HYNHHTVNLIDSPGHIDFCGEVSTAARLSDGALLLVDAVEGVHIQT-H 121
++++ HY H +D PGH D+ + T A DGA+L+V +G QT
Sbjct: 481 NTATVEYETETRHYAH-----VDCPGHADYVKNMITGAAQMDGAILVVSGADGPMPQTKE 645
Query: 122 AVLRQCWTEMLEPCLVLNKMDRLITELNLTPLEAYTRLLRIVHEVNGIWSAYNSEKYLSD 181
+L + + LNK D++ E L +E R L +E G S L
Sbjct: 646 HILLAKQVGVPNVVVFLNKQDQVDDEELLELVELEVRELLSSYEFPGDDIPITSGSALLA 825
Query: 182 VDALLAGGTAAGGEVMEDYDDVEDKFQPQKGNV 214
++AL+A GE +D DK NV
Sbjct: 826 LEALMANPAIKRGE-----NDWVDKIYQLMDNV 909
>TC9899 homologue to PIR|S35701|S35701 translation elongation factor EF-G,
chloroplast - soybean {Glycine max;} , partial (15%)
Length = 624
Score = 56.2 bits (134), Expect = 9e-09
Identities = 26/76 (34%), Positives = 46/76 (60%)
Frame = +1
Query: 12 KKIRNICILAHVDHGKTTLADQLIAAASGGMVHPKVAGKVRFMDYLDEEQRRAITMKSSS 71
K RNI I+AH+D GKTT ++++ +V MD++++EQ R IT+ S++
Sbjct: 397 KDYRNIGIMAHIDAGKTTTTERILFYTGRNYKIGEVHEGTATMDWMEQEQERGITITSAA 576
Query: 72 ISLHYNHHTVNLIDSP 87
+ +N H +++ID+P
Sbjct: 577 TTTFWNKHRIHIIDTP 624
>BP070882
Length = 496
Score = 53.1 bits (126), Expect = 8e-08
Identities = 35/104 (33%), Positives = 53/104 (50%), Gaps = 7/104 (6%)
Frame = +2
Query: 16 NICILAHVDHGKTTLADQLIAAASGGMVHPKVAGKVRF--MDYLDEEQRRAITMKSS--- 70
N+ + HVDHGKTTL AA + + A + F +D EE++R IT+ ++
Sbjct: 209 NVGTMGHVDHGKTTLT----AAITKVLADEGKAKAIAFDEIDKAPEEKKRGITIATAHVE 376
Query: 71 --SISLHYNHHTVNLIDSPGHIDFCGEVSTAARLSDGALLLVDA 112
++ HY H +D PGH D+ + T A DG +L+V A
Sbjct: 377 YETVKRHYAH-----VDCPGHADYVKNMITGAAQMDGGILVVSA 493
>BP069475
Length = 442
Score = 48.9 bits (115), Expect = 1e-06
Identities = 26/112 (23%), Positives = 54/112 (48%)
Frame = +2
Query: 252 WGPWYYNPKTKMIVGKKGISGSKARPMFVQFVLEPLWQVYQGALGGGKGMVEKVIKSFNL 311
WG +Y++P T+ K +SG + FV+FVLEPL+++Y +G K VE + +
Sbjct: 44 WGDYYFHPDTRTFKKKPPVSGGERS--FVEFVLEPLYKIYSQVIGEHKKSVETTLAELGV 217
Query: 312 QIQARELQNKDSKVVLQAVMSRWLPLSDAILSMVLKCLPDPVEGQKSRISRL 363
+ + + + +L+ S + M+++ +P P + ++ +
Sbjct: 218 TL-SNAAYRLNVRPLLRLACSSVFGQASGFTDMLVQHIPSPRDAAVKKVDHI 370
>AV408944
Length = 295
Score = 42.0 bits (97), Expect = 2e-04
Identities = 17/28 (60%), Positives = 23/28 (81%)
Frame = +1
Query: 11 RKKIRNICILAHVDHGKTTLADQLIAAA 38
+ IRN+ ++AHVDHGK+TL D L+AAA
Sbjct: 199 KHNIRNMSVIAHVDHGKSTLTDSLVAAA 282
>AV409888
Length = 426
Score = 39.7 bits (91), Expect = 9e-04
Identities = 18/41 (43%), Positives = 27/41 (64%), Gaps = 6/41 (14%)
Frame = +2
Query: 1 MEESTSDNNDRKK------IRNICILAHVDHGKTTLADQLI 35
+ E+T ++K+ +RNI I+AHVDHGKTTL D ++
Sbjct: 278 VSEATEPKTEKKQFTRRGDVRNIAIVAHVDHGKTTLVDAML 400
>AV769010
Length = 369
Score = 33.5 bits (75), Expect = 0.063
Identities = 18/41 (43%), Positives = 26/41 (62%), Gaps = 5/41 (12%)
Frame = +3
Query: 17 ICILAHVDHGKTTLAD-----QLIAAASGGMVHPKVAGKVR 52
I I+ HVDHGKTTL D +++A+ +GG+ A KV+
Sbjct: 192 ITIMGHVDHGKTTLLDYIRKSKVVASEAGGITQGIGAYKVQ 314
>TC10571 homologue to PIR|T00956|T00956 translation initiation factor eIF-2
gamma chain F20D22.6 - Arabidopsis
thaliana {Arabidopsis thaliana;}, partial (56%)
Length = 965
Score = 29.6 bits (65), Expect = 0.90
Identities = 22/64 (34%), Positives = 31/64 (48%), Gaps = 2/64 (3%)
Frame = +1
Query: 81 VNLIDSPGHIDFCGEVSTAARLSDGALLLVDAVEGV-HIQTHAVLRQCWTEMLEPCLVL- 138
V+ +D PGH + A + DGALLL+ A E QT L L+ ++L
Sbjct: 532 VSFVDCPGHDILMATMLNGAAIMDGALLLIAANESCPQPQTSEHLAAVEIMRLQHIIILQ 711
Query: 139 NKMD 142
NK+D
Sbjct: 712 NKVD 723
>BI419132
Length = 543
Score = 28.1 bits (61), Expect = 2.6
Identities = 11/25 (44%), Positives = 17/25 (68%)
Frame = -1
Query: 360 ISRLIPERKVGSENGVDRRVVEESE 384
++ ++ E KVG E G+ RVVE+ E
Sbjct: 120 LNDVVEEEKVGEERGIHERVVEKEE 46
>TC20102
Length = 540
Score = 28.1 bits (61), Expect = 2.6
Identities = 12/21 (57%), Positives = 15/21 (71%)
Frame = +1
Query: 277 PMFVQFVLEPLWQVYQGALGG 297
P F VLE LW+V++G LGG
Sbjct: 358 PWFYGRVLEILWEVFRGYLGG 420
>AV410803
Length = 135
Score = 26.6 bits (57), Expect = 7.7
Identities = 9/16 (56%), Positives = 13/16 (81%)
Frame = +3
Query: 11 RKKIRNICILAHVDHG 26
+ IRN+ ++AHVDHG
Sbjct: 87 KHNIRNMSVIAHVDHG 134
>BP070821
Length = 460
Score = 26.2 bits (56), Expect = 10.0
Identities = 14/51 (27%), Positives = 25/51 (48%), Gaps = 2/51 (3%)
Frame = -2
Query: 198 EDYDDVEDKFQPQKGNVVFACALDGWGFG--IHEFAEIYASKLGGSASVGA 246
+ + + K GN F+ + GWG+G +E + ++ +L AS GA
Sbjct: 174 KSFRNFRPKMMDSSGNCDFSACIAGWGYGRMNNETLDSFSIRLK*KASRGA 22
>AV770351
Length = 284
Score = 26.2 bits (56), Expect = 10.0
Identities = 21/63 (33%), Positives = 34/63 (53%), Gaps = 3/63 (4%)
Frame = +2
Query: 52 RFMDYLDEEQRRAIT---MKSSSISLHYNHHTVNLIDSPGHIDFCGEVSTAARLSDGALL 108
R+M+ D++ AIT +K S+ ++ N+H+ NLI PG E T R +DG +
Sbjct: 47 RYME--DKQLTTAITKTNIKFSTYIINSNNHSKNLIQIPG----LNE*RTRDRHADGKVS 208
Query: 109 LVD 111
+ D
Sbjct: 209 IWD 217
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.318 0.136 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,298,467
Number of Sequences: 28460
Number of extensions: 78874
Number of successful extensions: 416
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 403
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 404
length of query: 392
length of database: 4,897,600
effective HSP length: 92
effective length of query: 300
effective length of database: 2,279,280
effective search space: 683784000
effective search space used: 683784000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 56 (26.2 bits)
Medicago: description of AC146552.2