BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146549.14 + phase: 0 /pseudo/partial
(221 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC10000 28 0.96
TC13087 similar to UP|Q9FIF8 (Q9FIF8) Serine protease-like prote... 28 1.2
BP068408 27 2.8
TC17617 weakly similar to UP|Q84SA8 (Q84SA8) Laccase (Fragment),... 27 2.8
BP058134 27 3.6
TC10337 similar to GB|AAL06978.1|15810105|AY056090 AT4g37830/T28... 26 4.7
TC17723 26 4.7
TC16272 similar to GB|CAD40682.1|21741506|OSJN00124 OSJNBb0118P1... 26 6.2
TC13701 similar to UP|Q9SIR4 (Q9SIR4) At2g25290 protein, partial... 26 6.2
TC9369 similar to UP|Q93X72 (Q93X72) Leucine-rich repeat resista... 26 6.2
TC12549 similar to GB|AAO11541.1|27363244|BT002625 At1g79930/F19... 26 6.2
TC17823 similar to UP|Q93XN3 (Q93XN3) Salicylic acid-induced fra... 26 6.2
BP069981 25 8.1
TC11003 similar to UP|MYOP_MESCR (O49071) Inositol-1(or 4)-monop... 25 8.1
AV773671 25 8.1
TC18127 25 8.1
TC19504 similar to UP|Q9SV89 (Q9SV89) Isoleucine-tRNA ligase-lik... 25 8.1
TC17030 similar to UP|Q8S995 (Q8S995) Glucosyltransferase-14, pa... 25 8.1
TC11765 similar to UP|Q8L7W7 (Q8L7W7) AT3g01820/F28J7_15, partia... 25 8.1
>TC10000
Length = 934
Score = 28.5 bits (62), Expect = 0.96
Identities = 11/27 (40%), Positives = 15/27 (54%)
Frame = -2
Query: 97 WGWYQLEPVNQWLLLLLSMQLKCLRHT 123
WGWY L P ++LL S ++ C T
Sbjct: 930 WGWYYLTPSKNVIILLGSYKIYCRNST 850
>TC13087 similar to UP|Q9FIF8 (Q9FIF8) Serine protease-like protein, partial
(31%)
Length = 1036
Score = 28.1 bits (61), Expect = 1.2
Identities = 13/39 (33%), Positives = 23/39 (58%), Gaps = 1/39 (2%)
Frame = +1
Query: 121 RHTHICRILSIRSSHRFIISTLCHRANLR-FLSMPSLNR 158
+H+H C++L R+ HR S++C + ++ L P L R
Sbjct: 73 KHSHWCKLLRTRTRHRKRCSSICKDSCIQSMLPKPRLRR 189
>BP068408
Length = 473
Score = 26.9 bits (58), Expect = 2.8
Identities = 23/83 (27%), Positives = 35/83 (41%), Gaps = 6/83 (7%)
Frame = -2
Query: 84 GMVMDTTRRKKLKWGWYQLEPVNQWLLLLLSMQLKCLRHTHICRILSIRSSHRF------ 137
G ++D + +W W + V + L+ L + HT +C I RF
Sbjct: 385 GYIVDLYHSQAKRW*WSYV--VKWFKLIQL*YAVPLFHHTGLCLI-------RFSM*LFV 233
Query: 138 IISTLCHRANLRFLSMPSLNRYN 160
I+S+LCH L F +NR N
Sbjct: 232 ILSSLCHCP*LLFSVKTPINREN 164
>TC17617 weakly similar to UP|Q84SA8 (Q84SA8) Laccase (Fragment), partial
(53%)
Length = 1139
Score = 26.9 bits (58), Expect = 2.8
Identities = 12/36 (33%), Positives = 24/36 (66%), Gaps = 4/36 (11%)
Frame = +3
Query: 108 WLLLLLSMQLKCLRHTHIC----RILSIRSSHRFII 139
WL LL+++ +KCL +H+ I+++ ++HR I+
Sbjct: 48 WLHLLINLVVKCLIISHLVV*SFMIINMHNTHRHIL 155
>BP058134
Length = 545
Score = 26.6 bits (57), Expect = 3.6
Identities = 12/35 (34%), Positives = 20/35 (56%)
Frame = +2
Query: 109 LLLLLSMQLKCLRHTHICRILSIRSSHRFIISTLC 143
L LLS+ L+ L+ HIC +L+ + ++ LC
Sbjct: 413 LFFLLSIYLELLKMIHICELLTSSK*IKLMMKMLC 517
>TC10337 similar to GB|AAL06978.1|15810105|AY056090 AT4g37830/T28I19_110
{Arabidopsis thaliana;}, partial (73%)
Length = 617
Score = 26.2 bits (56), Expect = 4.7
Identities = 12/26 (46%), Positives = 18/26 (69%)
Frame = +1
Query: 117 LKCLRHTHICRILSIRSSHRFIISTL 142
++ LRHTHIC I + R+SH ++ L
Sbjct: 262 MRSLRHTHIC-ISATRNSHGVLMGYL 336
>TC17723
Length = 842
Score = 26.2 bits (56), Expect = 4.7
Identities = 16/48 (33%), Positives = 25/48 (51%)
Frame = -1
Query: 139 ISTLCHRANLRFLSMPSLNRYNINCRFNNNNKISKLGLLSLRYRCYML 186
+S H + L+F + L +C+FN + SKLG L L C++L
Sbjct: 803 LSFASHASFLQFETEVKLPIS*FSCKFNFYTESSKLGQLGLLG*CHLL 660
>TC16272 similar to GB|CAD40682.1|21741506|OSJN00124 OSJNBb0118P14.20 {Oryza
sativa (japonica cultivar-group);} , partial (52%)
Length = 545
Score = 25.8 bits (55), Expect = 6.2
Identities = 16/42 (38%), Positives = 22/42 (52%), Gaps = 4/42 (9%)
Frame = -1
Query: 113 LSMQLKCLRHTHICRILSIR----SSHRFIISTLCHRANLRF 150
L++ ++ IC L+I+ SSH FII L RAN F
Sbjct: 488 LNLAIRIT*FVQICSSLNIKNKLCSSHDFIICHLSKRANTSF 363
>TC13701 similar to UP|Q9SIR4 (Q9SIR4) At2g25290 protein, partial (16%)
Length = 432
Score = 25.8 bits (55), Expect = 6.2
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = +2
Query: 33 RP*RKIMLRG*SSLPRTTFQRWSLW 57
R *R +MLR L + F+RW LW
Sbjct: 23 RR*RPMMLRSCLKLLQVNFKRWLLW 97
>TC9369 similar to UP|Q93X72 (Q93X72) Leucine-rich repeat resistance
protein-like protein, partial (70%)
Length = 859
Score = 25.8 bits (55), Expect = 6.2
Identities = 13/23 (56%), Positives = 15/23 (64%)
Frame = -3
Query: 164 RFNNNNKISKLGLLSLRYRCYML 186
RF ++ IS L LL LRYRC L
Sbjct: 221 RFASSLGISPLKLLKLRYRCVKL 153
>TC12549 similar to GB|AAO11541.1|27363244|BT002625 At1g79930/F19K16_11
{Arabidopsis thaliana;}, partial (25%)
Length = 677
Score = 25.8 bits (55), Expect = 6.2
Identities = 16/42 (38%), Positives = 24/42 (57%), Gaps = 2/42 (4%)
Frame = +2
Query: 90 TRRKKLKWGW--YQLEPVNQWLLLLLSMQLKCLRHTHICRIL 129
+RRKKLK+ QLE V +W L++L L + C++L
Sbjct: 122 SRRKKLKFQLPKSQLEKVPRWKLMMLLHHLAPMTMMLTCKML 247
>TC17823 similar to UP|Q93XN3 (Q93XN3) Salicylic acid-induced fragment 1
protein (Fragment), partial (32%)
Length = 363
Score = 25.8 bits (55), Expect = 6.2
Identities = 12/28 (42%), Positives = 15/28 (52%)
Frame = +2
Query: 124 HICRILSIRSSHRFIISTLCHRANLRFL 151
H+CR+ I SSH L H +L FL
Sbjct: 242 HLCRLFIILSSHYCFAPPLHHAKSLDFL 325
>BP069981
Length = 409
Score = 25.4 bits (54), Expect = 8.1
Identities = 12/20 (60%), Positives = 13/20 (65%)
Frame = +1
Query: 119 CLRHTHICRILSIRSSHRFI 138
C HTHI ILS +SSH I
Sbjct: 166 CSYHTHILVILS*KSSHNSI 225
>TC11003 similar to UP|MYOP_MESCR (O49071) Inositol-1(or 4)-monophosphatase
(IMPase) (IMP) (Inositol monophosphatase) , partial
(54%)
Length = 553
Score = 25.4 bits (54), Expect = 8.1
Identities = 27/97 (27%), Positives = 38/97 (38%)
Frame = +3
Query: 44 SSLPRTTFQRWSLWEPVLRKPSGMESLCLRKLNLLRAHRRGMVMDTTRRKKLKWGWYQLE 103
SS P Q+W L + R S + S LRKL L +TR+ L
Sbjct: 48 SSAPSFLSQQWRLTKNQKRHSSHLPSTLLRKLVSL------FEKGSTRQNTLS------- 188
Query: 104 PVNQWLLLLLSMQLKCLRHTHICRILSIRSSHRFIIS 140
+WL+ LK ++H +LS S+ R S
Sbjct: 189 -TKEWLI----WSLKLIKHVKNSSLLSSSSNTRLTSS 284
>AV773671
Length = 453
Score = 25.4 bits (54), Expect = 8.1
Identities = 18/57 (31%), Positives = 24/57 (41%)
Frame = +2
Query: 151 LSMPSLNRYNINCRFNNNNKISKLGLLSLRYRCYMLSCFQLYSREGIVQLDRVSPHL 207
L++ L + NC F N LGLL + C++ Q QLD V HL
Sbjct: 41 LTLVELVAFLSNCLFT*RNFFKILGLLFFLFTCFIFKISQSLQ----*QLDEVWSHL 199
>TC18127
Length = 912
Score = 25.4 bits (54), Expect = 8.1
Identities = 12/37 (32%), Positives = 19/37 (50%), Gaps = 5/37 (13%)
Frame = +2
Query: 104 PVNQWLLLLLSM-----QLKCLRHTHICRILSIRSSH 135
PV+ WL + +M ++CL + H CR L + H
Sbjct: 185 PVDIWLSMFWAMLHSNGTMQCLVNEHYCRSLKAMAQH 295
>TC19504 similar to UP|Q9SV89 (Q9SV89) Isoleucine-tRNA ligase-like protein
, partial (11%)
Length = 491
Score = 25.4 bits (54), Expect = 8.1
Identities = 11/31 (35%), Positives = 16/31 (51%)
Frame = -1
Query: 105 VNQWLLLLLSMQLKCLRHTHICRILSIRSSH 135
+ +W LL L M+ CLR +L +R H
Sbjct: 137 IREWCLLALGMRSPCLRKLPPLLMLLVRFQH 45
>TC17030 similar to UP|Q8S995 (Q8S995) Glucosyltransferase-14, partial (51%)
Length = 1098
Score = 25.4 bits (54), Expect = 8.1
Identities = 12/30 (40%), Positives = 19/30 (63%)
Frame = -2
Query: 45 SLPRTTFQRWSLWEPVLRKPSGMESLCLRK 74
S+ TF R +LW P+L + + ++ LC RK
Sbjct: 695 SVSEVTFWR-TLWRPML*RQNSVKQLCPRK 609
>TC11765 similar to UP|Q8L7W7 (Q8L7W7) AT3g01820/F28J7_15, partial (38%)
Length = 468
Score = 25.4 bits (54), Expect = 8.1
Identities = 19/48 (39%), Positives = 25/48 (51%)
Frame = +2
Query: 46 LPRTTFQRWSLWEPVLRKPSGMESLCLRKLNLLRAHRRGMVMDTTRRK 93
+PR+T + LW E+L LR+L LR +RRG MDT K
Sbjct: 338 VPRSTSR*RMLWIK--------ENLSLRRL-FLRCYRRGWRMDTIEVK 454
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.340 0.147 0.491
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,949,917
Number of Sequences: 28460
Number of extensions: 84107
Number of successful extensions: 818
Number of sequences better than 10.0: 38
Number of HSP's better than 10.0 without gapping: 815
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 818
length of query: 221
length of database: 4,897,600
effective HSP length: 87
effective length of query: 134
effective length of database: 2,421,580
effective search space: 324491720
effective search space used: 324491720
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.9 bits)
S2: 53 (25.0 bits)
Medicago: description of AC146549.14