BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146548.3 + phase: 0
(173 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC11407 127 1e-30
AV423335 67 2e-12
TC15122 similar to UP|Q94AY5 (Q94AY5) At1g19950/T20H2_25, partia... 63 3e-11
TC10422 similar to UP|Q944P7 (Q944P7) AT4g30920/F6I18_170, parti... 30 0.30
TC11018 homologue to UP|Q8VWW1 (Q8VWW1) LIM domain protein, part... 29 0.50
BP051662 27 1.5
TC10665 weakly similar to UP|Q41419 (Q41419) Sucrolytic enzyme/f... 27 1.9
TC16503 homologue to UP|HKL3_MALDO (O04136) Homeobox protein kno... 27 2.5
TC8968 homologue to UP|Q41619 (Q41619) Protein kinase (Fragment)... 27 2.5
TC18341 similar to UP|Q25936 (Q25936) Major merozoite surface an... 27 2.5
AV776921 25 7.3
TC8641 similar to UP|Q9C5N6 (Q9C5N6) Protein synthesis initiatio... 25 7.3
TC17353 similar to UP|Q93ZI1 (Q93ZI1) AT3g12300/F28J15_117, part... 25 7.3
BP074579 25 7.3
BP072467 25 9.5
TC12364 similar to UP|Q9FJM0 (Q9FJM0) GTP binding protein-like, ... 25 9.5
>TC11407
Length = 669
Score = 127 bits (318), Expect = 1e-30
Identities = 59/76 (77%), Positives = 66/76 (86%)
Frame = +3
Query: 98 AYVYEHYVRPFLANPQTINIWYVPRKKDVFTKQDDIITAAEKYIKENGTEAFENLIHRAD 157
+YVYEHYVR FL PQTINIWYVPRKKD+F+K DDIITAAEKYI+ENGT+AFENLIHRA
Sbjct: 3 SYVYEHYVRAFLLXPQTINIWYVPRKKDIFSKPDDIITAAEKYIQENGTQAFENLIHRAG 182
Query: 158 KSKWGSSHHTMYDETY 173
KS+ +H MYDETY
Sbjct: 183 KSR-SDGYHAMYDETY 227
Score = 25.8 bits (55), Expect = 4.3
Identities = 12/32 (37%), Positives = 20/32 (62%)
Frame = +1
Query: 142 KENGTEAFENLIHRADKSKWGSSHHTMYDETY 173
++NG + +++ A KS+ +H MYDETY
Sbjct: 256 RDNGMHVYASML-LAGKSR-SDGYHAMYDETY 345
>AV423335
Length = 466
Score = 66.6 bits (161), Expect = 2e-12
Identities = 29/87 (33%), Positives = 48/87 (54%)
Frame = +1
Query: 27 ISLVYPLYASVRAIESKSPVDDQQWLTYWVLYSLITLFELTFAKILEWIPIWPYAKLILT 86
+ + P+Y++ +AIESK + L YW Y +L E+ K++ W+P++ + K
Sbjct: 115 VGIALPVYSTFKAIESKDQDAQNRCLLYWAAYGSFSLVEVFTDKLISWVPMYYHVKFAFL 294
Query: 87 CWLVLPYFTGAAYVYEHYVRPFLANPQ 113
WL LP GA +Y ++RPFL+ Q
Sbjct: 295 VWLQLPPTNGAKQLYVKHLRPFLSRHQ 375
>TC15122 similar to UP|Q94AY5 (Q94AY5) At1g19950/T20H2_25, partial (56%)
Length = 1343
Score = 62.8 bits (151), Expect = 3e-11
Identities = 27/87 (31%), Positives = 47/87 (53%), Gaps = 2/87 (2%)
Frame = +3
Query: 26 VISLVYPLYASVRAIESKSPVDDQQ--WLTYWVLYSLITLFELTFAKILEWIPIWPYAKL 83
++ YP Y +A+E P +Q W YW+L +++T+ E + W+P++ AKL
Sbjct: 192 ILGYAYPAYECYKAVEKNKPEIEQLRFWCQYWILVAVLTVCERIGDTFISWVPMYSEAKL 371
Query: 84 ILTCWLVLPYFTGAAYVYEHYVRPFLA 110
+ +L P G YVY+ + RP++A
Sbjct: 372 LFFIFLWYPKTKGTTYVYDSFFRPYVA 452
>TC10422 similar to UP|Q944P7 (Q944P7) AT4g30920/F6I18_170, partial (47%)
Length = 874
Score = 29.6 bits (65), Expect = 0.30
Identities = 20/55 (36%), Positives = 28/55 (50%), Gaps = 2/55 (3%)
Frame = +3
Query: 110 ANPQTINIWYVPRKKDVFTKQDDIITA--AEKYIKENGTEAFENLIHRADKSKWG 162
AN +T I + P+ DV + DI+ AEK + +G FEN+I SK G
Sbjct: 9 ANVETHKISFTPKDVDVTEWKGDILAVGVAEKDLVRDGKLRFENVILNKIDSKLG 173
>TC11018 homologue to UP|Q8VWW1 (Q8VWW1) LIM domain protein, partial (31%)
Length = 479
Score = 28.9 bits (63), Expect = 0.50
Identities = 16/76 (21%), Positives = 35/76 (46%), Gaps = 3/76 (3%)
Frame = +3
Query: 41 ESKSPVDDQQWLTYWVLYSLITLFELTFAKILEWIPIWPYAKLI---LTCWLVLPYFTGA 97
+SK Q L W L L + F ++ + +WP+ ++ L +L+ P +G
Sbjct: 144 QSKGQQPQFQNLDCWCLVLLSIFLKKIFGLSIKCLYVWPFFSIVL**LDPFLIFPSMSGV 323
Query: 98 AYVYEHYVRPFLANPQ 113
++ + +++ +N Q
Sbjct: 324 DFLCDLFIQCTFSNDQ 371
>BP051662
Length = 432
Score = 27.3 bits (59), Expect = 1.5
Identities = 10/34 (29%), Positives = 19/34 (55%)
Frame = +3
Query: 55 WVLYSLITLFELTFAKILEWIPIWPYAKLILTCW 88
W+ Y+L+ F +++ K IW + +L+CW
Sbjct: 312 WIFYALLPFFSISWVKQHSSTGIW--YRAVLSCW 407
>TC10665 weakly similar to UP|Q41419 (Q41419) Sucrolytic enzyme/ferredoxin
homolog protein, partial (48%)
Length = 902
Score = 26.9 bits (58), Expect = 1.9
Identities = 9/32 (28%), Positives = 19/32 (59%)
Frame = +1
Query: 45 PVDDQQWLTYWVLYSLITLFELTFAKILEWIP 76
P+D + +T+++L S T+ + F + W+P
Sbjct: 775 PIDIVRKITFFILKSFNTIMTIVFVSLRYWLP 870
>TC16503 homologue to UP|HKL3_MALDO (O04136) Homeobox protein knotted-1 like
3 (KNAP3), partial (22%)
Length = 723
Score = 26.6 bits (57), Expect = 2.5
Identities = 12/47 (25%), Positives = 23/47 (48%)
Frame = +2
Query: 35 ASVRAIESKSPVDDQQWLTYWVLYSLITLFELTFAKILEWIPIWPYA 81
A+ I +S + Q WLT W+ +S I + + ++ +W Y+
Sbjct: 449 ATKSEISIRSYIQKQNWLTPWMCFSYIVMLNEIYI-VVRGCGLWVYS 586
>TC8968 homologue to UP|Q41619 (Q41619) Protein kinase (Fragment), partial
(32%)
Length = 612
Score = 26.6 bits (57), Expect = 2.5
Identities = 12/25 (48%), Positives = 16/25 (64%)
Frame = -3
Query: 141 IKENGTEAFENLIHRADKSKWGSSH 165
++ENG A E L+H A + K GS H
Sbjct: 223 VEENGRLASECLVHEAHQRKDGSRH 149
>TC18341 similar to UP|Q25936 (Q25936) Major merozoite surface antigen
(Fragment), partial (5%)
Length = 497
Score = 26.6 bits (57), Expect = 2.5
Identities = 12/28 (42%), Positives = 15/28 (52%)
Frame = -2
Query: 51 WLTYWVLYSLITLFELTFAKILEWIPIW 78
W ++WV YS+ LF F W PIW
Sbjct: 244 WWSWWVHYSIFRLFRNLFCY*R*W-PIW 164
>AV776921
Length = 435
Score = 25.0 bits (53), Expect = 7.3
Identities = 9/27 (33%), Positives = 19/27 (70%)
Frame = +2
Query: 1 MGGSGSGAGSFLMVLLRNFDVLAGPVI 27
+GGSGSG + + ++ R ++ L+G ++
Sbjct: 92 VGGSGSGKSTVISLIERFYEPLSGDIL 172
>TC8641 similar to UP|Q9C5N6 (Q9C5N6) Protein synthesis initiation factor
eIF2 alpha, partial (41%)
Length = 628
Score = 25.0 bits (53), Expect = 7.3
Identities = 15/53 (28%), Positives = 24/53 (44%)
Frame = -3
Query: 11 FLMVLLRNFDVLAGPVISLVYPLYASVRAIESKSPVDDQQWLTYWVLYSLITL 63
FL + N+ V+ P+ + P+ S + S SP+ +YS ITL
Sbjct: 446 FLAIPTTNYSVITEPLSTSTSPIPVSSSSSLSSSPLTSSFSWRSLAMYSAITL 288
>TC17353 similar to UP|Q93ZI1 (Q93ZI1) AT3g12300/F28J15_117, partial (85%)
Length = 616
Score = 25.0 bits (53), Expect = 7.3
Identities = 11/41 (26%), Positives = 23/41 (55%)
Frame = +2
Query: 39 AIESKSPVDDQQWLTYWVLYSLITLFELTFAKILEWIPIWP 79
++E+ S + Q+ + W+ + +I +E TFA + + WP
Sbjct: 107 SLETNSAENVQEHIPIWISFHIIQPWEQTFADMGQRSCEWP 229
>BP074579
Length = 403
Score = 25.0 bits (53), Expect = 7.3
Identities = 5/16 (31%), Positives = 13/16 (81%)
Frame = +3
Query: 88 WLVLPYFTGAAYVYEH 103
W ++P F+G +++++H
Sbjct: 150 WFLMPQFSGTSFIFQH 197
>BP072467
Length = 549
Score = 24.6 bits (52), Expect = 9.5
Identities = 8/22 (36%), Positives = 14/22 (63%)
Frame = -3
Query: 68 FAKILEWIPIWPYAKLILTCWL 89
FA + +P+W YA L+ C++
Sbjct: 133 FAPVKGMLPVWYYASLM*ACYI 68
>TC12364 similar to UP|Q9FJM0 (Q9FJM0) GTP binding protein-like, partial
(6%)
Length = 418
Score = 24.6 bits (52), Expect = 9.5
Identities = 13/51 (25%), Positives = 24/51 (46%)
Frame = -2
Query: 70 KILEWIPIWPYAKLILTCWLVLPYFTGAAYVYEHYVRPFLANPQTINIWYV 120
K L+W+ + Y +L TC+ AA Y + P +N + ++Y+
Sbjct: 312 K*LQWLHTYIYVQLYETCY--------AASYYFSFPSPMTSNIKDAKLYYI 184
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.323 0.139 0.446
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,434,142
Number of Sequences: 28460
Number of extensions: 48170
Number of successful extensions: 267
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 266
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 266
length of query: 173
length of database: 4,897,600
effective HSP length: 84
effective length of query: 89
effective length of database: 2,506,960
effective search space: 223119440
effective search space used: 223119440
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 52 (24.6 bits)
Medicago: description of AC146548.3