BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146548.2 + phase: 0
(203 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC19923 211 7e-56
TC10640 weakly similar to UP|Q8RX65 (Q8RX65) AT4g36060/T19K4_190... 99 4e-22
BF177862 79 4e-16
TC10393 70 2e-13
TC12218 63 3e-11
TC18373 45 7e-06
TC14555 similar to UP|Q43558 (Q43558) Proline rich protein precu... 36 0.004
TC14298 homologue to UP|Q8L5W2 (Q8L5W2) bZIP transcription facto... 33 0.026
TC14866 similar to UP|Q8L5W2 (Q8L5W2) bZIP transcription factor ... 33 0.026
CB828313 32 0.100
TC17113 weakly similar to UP|Q9LFU8 (Q9LFU8) Proline-rich protei... 31 0.13
TC15102 similar to UP|Q7X9B3 (Q7X9B3) 9/13 hydroperoxide lyase, ... 30 0.22
TC15731 similar to UP|Q07526 (Q07526) Protein kinase PSPK-5 (Pr... 29 0.50
BP078507 29 0.50
TC17367 similar to GB|BAB03049.1|9294683|AP001305 syringomycin b... 29 0.50
TC14326 weakly similar to UP|EXLP_TOBAC (Q03211) Pistil-specific... 29 0.65
BI420016 29 0.65
TC8093 homologue to UP|EFTU_TOBAC (P41342) Elongation factor Tu,... 29 0.65
TC14969 similar to PIR|PQ0772|PQ0772 4-coumarate-CoA ligase (cl... 29 0.65
TC8096 weakly similar to GB|AAF89661.1|9622185|AF168782 acinusL ... 29 0.65
>TC19923
Length = 700
Score = 211 bits (537), Expect = 7e-56
Identities = 102/129 (79%), Positives = 113/129 (87%), Gaps = 2/129 (1%)
Frame = +1
Query: 74 ESHYVTMEKNELKEENSSLETQIEKLQGEIQARIAQSKPDLNAPPQLELESPEQTTFSGQ 133
ES+YVTM+KNELKEEN SLETQIEKLQGEIQAR+AQSKPDLNAPP LELE PEQT F GQ
Sbjct: 1 ESNYVTMKKNELKEENCSLETQIEKLQGEIQARVAQSKPDLNAPPHLELEPPEQTNFPGQ 180
Query: 134 SFQLPTVDPTLQQGPTVLVVPFRPDLQAAYPAP--TEITPNPPLVISKPHARYPTPADSW 191
S Q+PT++PTL QGP VLVVPFRPDLQ A+P P +E+ P P V+S+PHARYPTPADSW
Sbjct: 181 SIQVPTMEPTLPQGPAVLVVPFRPDLQTAFPVPNISELMPKPAPVVSQPHARYPTPADSW 360
Query: 192 PSQLLGEQP 200
PSQLLGEQP
Sbjct: 361 PSQLLGEQP 387
>TC10640 weakly similar to UP|Q8RX65 (Q8RX65) AT4g36060/T19K4_190, partial
(13%)
Length = 467
Score = 99.4 bits (246), Expect = 4e-22
Identities = 48/56 (85%), Positives = 51/56 (90%)
Frame = +2
Query: 1 MKQGKVPKRIHKAEREKMKREHLNELFLDLANALDLSEPNNGKASILIEASRLLKD 56
M QGK P+R+HKAEREKMKREHLNELFLDLAN LDL+E NNGKASIL EASRLLKD
Sbjct: 299 MNQGKAPRRVHKAEREKMKREHLNELFLDLANTLDLNEQNNGKASILCEASRLLKD 466
>BF177862
Length = 312
Score = 79.3 bits (194), Expect = 4e-16
Identities = 36/43 (83%), Positives = 39/43 (89%)
Frame = +3
Query: 1 MKQGKVPKRIHKAEREKMKREHLNELFLDLANALDLSEPNNGK 43
M QGK P+R+HKAEREKMKREHLNELFLDLAN LDL+E NNGK
Sbjct: 183 MNQGKAPRRVHKAEREKMKREHLNELFLDLANTLDLNEQNNGK 311
>TC10393
Length = 1063
Score = 70.5 bits (171), Expect = 2e-13
Identities = 40/109 (36%), Positives = 67/109 (60%), Gaps = 1/109 (0%)
Frame = +1
Query: 12 KAEREKMKREHLNELFLDLANALDLSEP-NNGKASILIEASRLLKDLLCQIQSLKKENVS 70
KA REK++R+ LN+ F++L + L+ P KA+ILI+A R++ L + Q +K N+
Sbjct: 433 KACREKLRRDKLNDKFVELGSILEPGRPPKTDKAAILIDAVRMVTQLRGEAQKMKDTNMG 612
Query: 71 LLSESHYVTMEKNELKEENSSLETQIEKLQGEIQARIAQSKPDLNAPPQ 119
L + + EKNEL++E L+T+ E+L+ ++++ AQ P PPQ
Sbjct: 613 LQEKIKELKTEKNELRDEKQRLKTEKERLEQQLKSMNAQ--PSFMPPPQ 753
>TC12218
Length = 737
Score = 63.2 bits (152), Expect = 3e-11
Identities = 35/99 (35%), Positives = 62/99 (62%), Gaps = 1/99 (1%)
Frame = +2
Query: 12 KAEREKMKREHLNELFLDLANALDLS-EPNNGKASILIEASRLLKDLLCQIQSLKKENVS 70
KA REK++R+ LNE FL+L++ L+ +P KA+I+ +A R++ L + + LK+ N
Sbjct: 278 KACREKLRRDKLNERFLELSSILEPGRQPKTDKAAIISDAVRVVTQLRNEAEKLKEMNND 457
Query: 71 LLSESHYVTMEKNELKEENSSLETQIEKLQGEIQARIAQ 109
L + + EKNE+++E + L+ EKL+ +++ R Q
Sbjct: 458 LQEKIKELKAEKNEIRDEKNKLKLDKEKLEKKVKLRNVQ 574
>TC18373
Length = 437
Score = 45.4 bits (106), Expect = 7e-06
Identities = 27/62 (43%), Positives = 37/62 (59%), Gaps = 1/62 (1%)
Frame = +1
Query: 12 KAEREKMKREHLNELFLDLANALDLSEP-NNGKASILIEASRLLKDLLCQIQSLKKENVS 70
KA REK +RE LNE F +L++ L+ P K +IL +A R+L L + Q LK+ N
Sbjct: 250 KACREKQRREKLNERFCELSSVLEPGRPVKTDKPAILDDAIRVLSQLKTEAQELKETNEK 429
Query: 71 LL 72
LL
Sbjct: 430 LL 435
>TC14555 similar to UP|Q43558 (Q43558) Proline rich protein precursor,
partial (43%)
Length = 1038
Score = 36.2 bits (82), Expect = 0.004
Identities = 23/68 (33%), Positives = 30/68 (43%)
Frame = +2
Query: 136 QLPTVDPTLQQGPTVLVVPFRPDLQAAYPAPTEITPNPPLVISKPHARYPTPADSWPSQL 195
Q P+ PT P P P Q++ P P + +P PP+ S P A P PA S P +
Sbjct: 185 QSPSNSPTTSPAPPTPTTPQPPAAQSS-PPPAQSSP-PPVQSSPPPASTPPPAQSSPPPV 358
Query: 196 LGEQPTSS 203
P S
Sbjct: 359 SSPPPVQS 382
>TC14298 homologue to UP|Q8L5W2 (Q8L5W2) bZIP transcription factor ATB2,
partial (62%)
Length = 1585
Score = 33.5 bits (75), Expect = 0.026
Identities = 15/52 (28%), Positives = 29/52 (54%)
Frame = +3
Query: 54 LKDLLCQIQSLKKENVSLLSESHYVTMEKNELKEENSSLETQIEKLQGEIQA 105
L DL Q+ L+KEN +++ + T + ++ ENS L Q+ +L +++
Sbjct: 768 LDDLASQVTQLRKENQQIITSVNITTQQYLSVEAENSVLRAQMGELSNRLES 923
>TC14866 similar to UP|Q8L5W2 (Q8L5W2) bZIP transcription factor ATB2,
partial (60%)
Length = 948
Score = 33.5 bits (75), Expect = 0.026
Identities = 16/52 (30%), Positives = 29/52 (55%)
Frame = +3
Query: 54 LKDLLCQIQSLKKENVSLLSESHYVTMEKNELKEENSSLETQIEKLQGEIQA 105
L+DL Q+ L KEN +L+ + + + ++ ENS L Q+ +L +Q+
Sbjct: 126 LEDLTSQVTQLTKENGEILTNINITSQQYQNVETENSILRAQMGELSQRLQS 281
>CB828313
Length = 541
Score = 31.6 bits (70), Expect = 0.100
Identities = 19/81 (23%), Positives = 40/81 (48%)
Frame = +2
Query: 90 SSLETQIEKLQGEIQARIAQSKPDLNAPPQLELESPEQTTFSGQSFQLPTVDPTLQQGPT 149
+S + + E++ARI + + + P+LE+++PE F+ ++ L+ +
Sbjct: 260 NSRDPSVTPFVDELRARILRHRHATTSNPKLEMDNPEDGGMKILPFEFVALEACLEAACS 439
Query: 150 VLVVPFRPDLQAAYPAPTEIT 170
VL + Q A+PA ++T
Sbjct: 440 VLENEAKTLEQEAHPALDKLT 502
>TC17113 weakly similar to UP|Q9LFU8 (Q9LFU8) Proline-rich protein, partial
(25%)
Length = 1077
Score = 31.2 bits (69), Expect = 0.13
Identities = 23/77 (29%), Positives = 35/77 (44%)
Frame = +1
Query: 117 PPQLELESPEQTTFSGQSFQLPTVDPTLQQGPTVLVVPFRPDLQAAYPAPTEITPNPPLV 176
PP L + +P Q FQ P + P Q P ++ PF+P P P + P+PP +
Sbjct: 403 PPPL-IPNPFQPPLVPNPFQPPPLIPNPFQPPPLIPNPFQPSPPPLLPDPFQ-PPSPPPL 576
Query: 177 ISKPHARYPTPADSWPS 193
P + P+P P+
Sbjct: 577 FPNPF-QPPSPPPLIPN 624
>TC15102 similar to UP|Q7X9B3 (Q7X9B3) 9/13 hydroperoxide lyase, partial
(68%)
Length = 1097
Score = 30.4 bits (67), Expect = 0.22
Identities = 15/41 (36%), Positives = 22/41 (53%)
Frame = +1
Query: 161 AAYPAPTEITPNPPLVISKPHARYPTPADSWPSQLLGEQPT 201
A PAP++ PNPP SKP + + ++ PS L P+
Sbjct: 415 ATAPAPSKTPPNPPTNSSKPSSCKSSLPNTTPSSLSSAPPS 537
>TC15731 similar to UP|Q07526 (Q07526) Protein kinase PSPK-5 (Probable
serine/threonine-protein kinase PK5) , partial (17%)
Length = 940
Score = 29.3 bits (64), Expect = 0.50
Identities = 16/51 (31%), Positives = 25/51 (48%), Gaps = 3/51 (5%)
Frame = -3
Query: 57 LLCQIQSLKKENVSLLSESHYVTMEKNELKEENSSLETQ---IEKLQGEIQ 104
L C ++ ++KE E+H + +KN+ LET I KLQ +Q
Sbjct: 560 LECMVEMMRKEKRDT*KENHKINYDKNKFTPSMGKLETSHHTIHKLQ*NMQ 408
>BP078507
Length = 414
Score = 29.3 bits (64), Expect = 0.50
Identities = 28/90 (31%), Positives = 35/90 (38%), Gaps = 6/90 (6%)
Frame = -1
Query: 111 KPDLNAP------PQLELESPEQTTFSGQSFQLPTVDPTLQQGPTVLVVPFRPDLQAAYP 164
+P NAP P +L P T S Q P PTL Q + P P + P
Sbjct: 267 QPSPNAPTSPYPAPSSQLRPPPST-----SHQPP---PTLLQPTSDTPSPSPPPPSSPNP 112
Query: 165 APTEITPNPPLVISKPHARYPTPADSWPSQ 194
P +PNPP P + P+P S Q
Sbjct: 111 PPPHHSPNPP----HPPSSSPSPPQSSQGQ 34
>TC17367 similar to GB|BAB03049.1|9294683|AP001305 syringomycin biosynthesis
enzyme-like protein {Arabidopsis thaliana;}, partial
(17%)
Length = 341
Score = 29.3 bits (64), Expect = 0.50
Identities = 22/78 (28%), Positives = 31/78 (39%), Gaps = 1/78 (1%)
Frame = +3
Query: 112 PDLNAPPQLELESPEQTTF-SGQSFQLPTVDPTLQQGPTVLVVPFRPDLQAAYPAPTEIT 170
P+L++PPQ L P Q F S Q Q +PT T F + P + +
Sbjct: 102 PELHSPPQYHLLRPPQLRFLSPQHSQSKPTNPTSNHSSTNPAPSFSETSHSTRPTTSTTS 281
Query: 171 PNPPLVISKPHARYPTPA 188
P + S P P+ A
Sbjct: 282 SRPSGMKSFPTLAAPSRA 335
>TC14326 weakly similar to UP|EXLP_TOBAC (Q03211) Pistil-specific
extensin-like protein precursor (PELP), partial (11%)
Length = 630
Score = 28.9 bits (63), Expect = 0.65
Identities = 21/82 (25%), Positives = 33/82 (39%), Gaps = 2/82 (2%)
Frame = +2
Query: 108 AQSKPDLNAPPQLE--LESPEQTTFSGQSFQLPTVDPTLQQGPTVLVVPFRPDLQAAYPA 165
A S P + +PP +++P + PT P + + P P + +
Sbjct: 251 APSPPIVKSPPYFPPPVKAPSPPIVKSPPVKAPT--PPIVKSPPSYPPPVKAPSPPIVKS 424
Query: 166 PTEITPNPPLVISKPHARYPTP 187
P P PP+V S P+ YP P
Sbjct: 425 PPVKAPTPPIVKSPPY--YPPP 484
Score = 26.9 bits (58), Expect = 2.5
Identities = 20/55 (36%), Positives = 25/55 (45%), Gaps = 2/55 (3%)
Frame = +2
Query: 140 VDPTLQQGPTVLVVPFRPDLQAAYPAPTEIT--PNPPLVISKPHARYPTPADSWP 192
V P P V VP P ++A P P P+PP+V S P+ P A S P
Sbjct: 158 VKPPTPSTPAV-TVPTPPLVKAPTPPPQPPVKAPSPPIVKSPPYFPPPVKAPSPP 319
Score = 25.8 bits (55), Expect = 5.5
Identities = 19/61 (31%), Positives = 25/61 (40%), Gaps = 6/61 (9%)
Frame = +2
Query: 138 PTVDPTLQQGPTVLVVP--FRPDLQAAYP----APTEITPNPPLVISKPHARYPTPADSW 191
P P P ++ P F P ++A P +P P PP+V S P P A S
Sbjct: 230 PPQPPVKAPSPPIVKSPPYFPPPVKAPSPPIVKSPPVKAPTPPIVKSPPSYPPPVKAPSP 409
Query: 192 P 192
P
Sbjct: 410 P 412
>BI420016
Length = 559
Score = 28.9 bits (63), Expect = 0.65
Identities = 27/111 (24%), Positives = 35/111 (31%), Gaps = 2/111 (1%)
Frame = +2
Query: 82 KNELKEENSSLETQIEKLQGEIQARIAQSKPDLNAPPQLELESPEQTTFSGQSFQ--LPT 139
+ E K NS+ T R P PQ + Q T G +P
Sbjct: 53 EEEYKNHNSNRNTAWNSTATTHLRRHQTPPP*APPEPQTLKKPTTQCTHGGNPSPKPVPA 232
Query: 140 VDPTLQQGPTVLVVPFRPDLQAAYPAPTEITPNPPLVISKPHARYPTPADS 190
P+ PT+ P P P P+PP S P + P P S
Sbjct: 233 STPSPPSSPTLTPPPSPPSPTPNAPLSPSSPPSPPTPPSPPPSPAPNPTPS 385
>TC8093 homologue to UP|EFTU_TOBAC (P41342) Elongation factor Tu,
chloroplast precursor (EF-Tu), partial (58%)
Length = 1146
Score = 28.9 bits (63), Expect = 0.65
Identities = 24/79 (30%), Positives = 30/79 (37%), Gaps = 7/79 (8%)
Frame = +2
Query: 124 SPEQTTFSGQSFQLPTVDPTLQ-------QGPTVLVVPFRPDLQAAYPAPTEITPNPPLV 176
+P FS S +P P LQ Q L++P P +TP PP
Sbjct: 11 NPPSIFFSSPS*SIPIPKPPLQSNQQWQFQQLLQLLLPPNSSTHTLLLLPLTLTPLPPAS 190
Query: 177 ISKPHARYPTPADSWPSQL 195
S P+ YP P PS L
Sbjct: 191 SSDPN--YPPPTSPPPSIL 241
>TC14969 similar to PIR|PQ0772|PQ0772 4-coumarate-CoA ligase (clone
GM4CL1B) - soybean (fragment) {Glycine
max;} , partial (70%)
Length = 928
Score = 28.9 bits (63), Expect = 0.65
Identities = 23/106 (21%), Positives = 43/106 (39%), Gaps = 2/106 (1%)
Frame = -3
Query: 99 LQGEIQARIAQSKPDLNAPPQLELESPEQ--TTFSGQSFQLPTVDPTLQQGPTVLVVPFR 156
L G + +I ++ + PP + P+Q SG+ + P+ + PT+
Sbjct: 719 LWGTVALQILRTWTEPALPPSFRIPVPKQLEAPCSGELPRAPSREAQPLTAPTLWS---- 552
Query: 157 PDLQAAYPAPTEITPNPPLVISKPHARYPTPADSWPSQLLGEQPTS 202
P P++ +P P L + P P ++S P+ L + S
Sbjct: 551 ---SGQTPPPSDSSPEPALAAAPPWPPTPCASESAPATTLSQTSAS 423
>TC8096 weakly similar to GB|AAF89661.1|9622185|AF168782 acinusL protein
{Mus musculus;} , partial (3%)
Length = 1044
Score = 28.9 bits (63), Expect = 0.65
Identities = 19/70 (27%), Positives = 29/70 (41%)
Frame = +1
Query: 119 QLELESPEQTTFSGQSFQLPTVDPTLQQGPTVLVVPFRPDLQAAYPAPTEITPNPPLVIS 178
+++LE+P S +PTV P P + +P P + P PPL +
Sbjct: 10 KMKLEAPPPPPPQAASVSVPTVAPAPSSSQPE---PSPRQQREQHPIPVALPPPPPLSRA 180
Query: 179 KPHARYPTPA 188
P AR P+
Sbjct: 181 PPVARERLPS 210
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.309 0.128 0.355
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,173,000
Number of Sequences: 28460
Number of extensions: 43993
Number of successful extensions: 383
Number of sequences better than 10.0: 88
Number of HSP's better than 10.0 without gapping: 360
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 379
length of query: 203
length of database: 4,897,600
effective HSP length: 86
effective length of query: 117
effective length of database: 2,450,040
effective search space: 286654680
effective search space used: 286654680
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 53 (25.0 bits)
Medicago: description of AC146548.2