BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146527.6 - phase: 0 /pseudo
(1019 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV780009 180 1e-45
BP043850 165 3e-41
BP066094 108 3e-26
BP075943 115 4e-26
TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%) 86 4e-17
BP084332 83 2e-16
AU088980 82 6e-16
AV424544 74 2e-13
AV427921 73 3e-13
TC15664 weakly similar to UP|O81617 (O81617) F8M12.17 protein, p... 72 4e-13
TC8952 similar to UP|O82607 (O82607) T2L5.9 protein, partial (3%) 67 2e-11
TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 65 4e-11
TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partia... 57 1e-08
BP057233 55 4e-08
AV766665 49 3e-06
TC10484 similar to UP|Q850H7 (Q850H7) Gag-pol polyprotein (Fragm... 35 3e-06
BP084259 49 5e-06
TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-rela... 47 1e-05
AV769206 47 2e-05
AV767042 46 3e-05
>AV780009
Length = 529
Score = 180 bits (456), Expect = 1e-45
Identities = 87/162 (53%), Positives = 113/162 (69%)
Frame = -1
Query: 848 AVQQIIRYLLGTLKRGLFFPVGSSIKLQAYSDADWAGCPDTRKSTTGWCMFLGNAPISWK 907
A +++RY+ G +GLFF S +KLQAYSD+DWAGCPDTR+S TG+ +FLG + ISW+
Sbjct: 526 AATRVLRYVKGAPAQGLFFSADSPLKLQAYSDSDWAGCPDTRRSVTGYSIFLGTSLISWR 347
Query: 908 CKKQDSVSKSSTEAEYRAMSAACSEIIWLRGLLTELGFSQDQPTPLHADNTSAIQIAANP 967
KKQ +VS+SS+EAEYRA++A E+ WL L L + P PL DN SA+ IA NP
Sbjct: 346 TKKQTTVSRSSSEAEYRALAATVCEVQWLSYLFQFLKLNVPLPVPLFCDNQSALHIAHNP 167
Query: 968 VYHERTKHIEVDCHSIREAYDRRIINLPHVSTSVQTADIFTK 1009
+HERTKHIE+DCH +R +I+L +ST Q ADIFTK
Sbjct: 166 TFHERTKHIELDCHVVRAKLQAGLIHLLPISTHHQLADIFTK 41
>BP043850
Length = 515
Score = 165 bits (418), Expect = 3e-41
Identities = 79/89 (88%), Positives = 83/89 (92%)
Frame = -1
Query: 931 SEIIWLRGLLTELGFSQDQPTPLHADNTSAIQIAANPVYHERTKHIEVDCHSIREAYDRR 990
SEIIWLRGLL+ELGF Q QPTPLHADNTSAIQIAANPVYHE T+HIEVDCHS+REAYDRR
Sbjct: 515 SEIIWLRGLLSELGFLQSQPTPLHADNTSAIQIAANPVYHEWTRHIEVDCHSVREAYDRR 336
Query: 991 IINLPHVSTSVQTADIFTKSLTRQRHNFL 1019
+I LPHVSTSVQ ADI TKSLTRQRHNFL
Sbjct: 335 VITLPHVSTSVQIADILTKSLTRQRHNFL 249
>BP066094
Length = 532
Score = 108 bits (271), Expect(2) = 3e-26
Identities = 53/149 (35%), Positives = 90/149 (59%), Gaps = 1/149 (0%)
Frame = +2
Query: 608 AKMTTVRTILAIAASQAWPLHQMDVKNAFLHGDLQEEVYIKLPNGMPTP-SPNTVCKLKR 666
+K +R +++ + + LHQM+VK+AFL+G + EEVY+ P G + + + KLK+
Sbjct: 86 SKTEAIRLLISFSVNHNIILHQMNVKSAFLNGYISEEVYVHQPPGXEDEKNSDHIFKLKK 265
Query: 667 SLYGLKQAPRVWFEKFRSTLLGFEFSQSRYDPSLFLQRTPKGMVVLLVYVDDIVVTGSDQ 726
SLYGLKQAPR W+E+ S LL E + + D +LF + ++++ +YVDDI+ ++
Sbjct: 266 SLYGLKQAPRAWYERLSSFLLENEXVRGKVDTTLFCKTYKDDILIVQIYVDDIIFGSANP 445
Query: 727 DAISRIKNLLHSTFHMKELGRLTYFLGLE 755
++ + F M+ +G L YFLG++
Sbjct: 446 SLCKEFSEMMQAEFEMRMMGELKYFLGIQ 532
Score = 27.7 bits (60), Expect(2) = 3e-26
Identities = 11/16 (68%), Positives = 14/16 (86%)
Frame = +3
Query: 594 QEYGLDYDETFAPVAK 609
Q+ G+DY ETFAPVA+
Sbjct: 45 QQ*GIDYTETFAPVAR 92
>BP075943
Length = 547
Score = 115 bits (288), Expect = 4e-26
Identities = 64/171 (37%), Positives = 95/171 (55%), Gaps = 11/171 (6%)
Frame = -3
Query: 712 LLVYVDDIVVTGSDQDAISRIKNLLHSTFHMKELGRLTYFLGLEVHYHHEGVFLNQQKYI 771
LL+YVDD+++ G+D I +K+ LH F +K+LG YFLGLE+ G+ LNQ+KY
Sbjct: 521 LLLYVDDVLLAGNDMHEIQLVKSSLHDQFRIKDLGEAKYFLGLEIARSTSGIVLNQRKYA 342
Query: 772 QDLVQLAGL-----------TNATLVDTPMEVNVKYRRDEGDHLDDPTQYRKLVGSLIYV 820
L+ +G TN+ + T + G L D YR++VG L+Y+
Sbjct: 341 LQLISDSGHFGFQPRFYSHGTNSQTLGT----------NTGTPLTDIGSYRRIVGRLLYL 192
Query: 821 TITRPDISFAVHTVSKFMQAPRHFHLSAVQQIIRYLLGTLKRGLFFPVGSS 871
TRPDI+FAV+ +S+F+ AP H + ++YL G+ GLF+P SS
Sbjct: 191 NTTRPDITFAVNQLSQFLSAPTDIHEQQLTGFLKYL*GSPGSGLFYPASSS 39
>TC16929 weakly similar to UP|Q9LFY6 (Q9LFY6) T7N9.5, partial (4%)
Length = 553
Score = 85.5 bits (210), Expect = 4e-17
Identities = 41/80 (51%), Positives = 56/80 (69%)
Frame = +1
Query: 935 WLRGLLTELGFSQDQPTPLHADNTSAIQIAANPVYHERTKHIEVDCHSIREAYDRRIINL 994
WL LL +L +QP ++ DN SA IAANPV+HERTKHIE+DCH +RE + +I+L
Sbjct: 4 WLTYLLQDLKVPFEQPALVYCDNNSARHIAANPVFHERTKHIEIDCHIVRERIQKGLIHL 183
Query: 995 PHVSTSVQTADIFTKSLTRQ 1014
+S+S ADI+TK+L+ Q
Sbjct: 184 LPISSSEPLADIYTKALSPQ 243
>BP084332
Length = 368
Score = 83.2 bits (204), Expect = 2e-16
Identities = 40/109 (36%), Positives = 65/109 (58%)
Frame = +2
Query: 911 QDSVSKSSTEAEYRAMSAACSEIIWLRGLLTELGFSQDQPTPLHADNTSAIQIAANPVYH 970
Q +++ S+ EAEY + + ++++W++ L + + P++ DNT+AI ++ NP+ H
Sbjct: 32 QSTIALSTAEAEYISAAICSTQMLWMKHQLEDYQILESN-IPIYCDNTAAISLSKNPILH 208
Query: 971 ERTKHIEVDCHSIREAYDRRIINLPHVSTSVQTADIFTKSLTRQRHNFL 1019
R KHIEV H IR+ + ++ L V T Q ADIFTK L R NF+
Sbjct: 209 SRAKHIEVKYHFIRDYVQKGVLLLKFVDTDHQWADIFTKPLAEDRFNFI 355
>AU088980
Length = 360
Score = 81.6 bits (200), Expect = 6e-16
Identities = 37/109 (33%), Positives = 58/109 (52%)
Frame = +2
Query: 307 NGVAERKNRHLLDVVRTLLLESHVPSRFWCEALSTAVHLINRMPSPSIGNESPFTRLYGH 366
N + ERK+RH+L+V R L+ S++P W A+ A +LIN +PSP + + P+ L
Sbjct: 2 NSIVERKHRHILNVTRALMFHSYLPKNLWTFAVKHAAYLINXLPSPLLKGQCPYQFLNND 181
Query: 367 PPNYSTLRVFGCVCYVHLPPQERTKFTAQSVECAFLGYSPHQKGFLCYD 415
P L+VFG +CY K ++ +C FLG+ G++ D
Sbjct: 182 SPTLLDLKVFGTLCYSSTLTHNXQKLDPRARKCVFLGFKTGTXGYIVMD 328
>AV424544
Length = 276
Score = 73.6 bits (179), Expect = 2e-13
Identities = 40/90 (44%), Positives = 55/90 (60%)
Frame = +3
Query: 681 KFRSTLLGFEFSQSRYDPSLFLQRTPKGMVVLLVYVDDIVVTGSDQDAISRIKNLLHSTF 740
K S L + QS +D SLF + V+LVYVDD+++ G+D + I +KN L F
Sbjct: 6 KLSSYLHILGYIQSAHDHSLFTKFRDASFTVILVYVDDLILAGNDLNEIQCVKNKLDIQF 185
Query: 741 HMKELGRLTYFLGLEVHYHHEGVFLNQQKY 770
+K+LG L YFLGLEV G+FL+Q+KY
Sbjct: 186 RIKDLGTLKYFLGLEVARSSCGLFLSQRKY 275
>AV427921
Length = 284
Score = 72.8 bits (177), Expect = 3e-13
Identities = 36/87 (41%), Positives = 53/87 (60%)
Frame = +1
Query: 303 TP*QNGVAERKNRHLLDVVRTLLLESHVPSRFWCEALSTAVHLINRMPSPSIGNESPFTR 362
TP QNGV+ERKNR +L++VR+LL S +P F EA+ ++H++NR P+ + N P
Sbjct: 22 TPQQNGVSERKNRTILNMVRSLLTMSGLPKSFLPEAVMWSLHILNRSPTLVVQNMMPEEA 201
Query: 363 LYGHPPNYSTLRVFGCVCYVHLPPQER 389
G P R+ C+ Y H+P Q+R
Sbjct: 202 WSGRQPAVDHFRISRCLAYAHVPDQKR 282
>TC15664 weakly similar to UP|O81617 (O81617) F8M12.17 protein, partial (4%)
Length = 670
Score = 72.0 bits (175), Expect = 4e-13
Identities = 35/80 (43%), Positives = 51/80 (63%)
Frame = +1
Query: 932 EIIWLRGLLTELGFSQDQPTPLHADNTSAIQIAANPVYHERTKHIEVDCHSIREAYDRRI 991
E+ WL +L +L P+ L+ D+ SA IA N V+HERTKH+++DCH +RE ++
Sbjct: 4 EL*WLTYILQDLRVPFISPSLLYCDSQSARHIATNAVFHERTKHLDIDCHVVREKLQAKL 183
Query: 992 INLPHVSTSVQTADIFTKSL 1011
+L +S+ QTADI TK L
Sbjct: 184 FHLLPISSVDQTADILTKPL 243
>TC8952 similar to UP|O82607 (O82607) T2L5.9 protein, partial (3%)
Length = 550
Score = 66.6 bits (161), Expect = 2e-11
Identities = 32/82 (39%), Positives = 53/82 (64%)
Frame = +3
Query: 932 EIIWLRGLLTELGFSQDQPTPLHADNTSAIQIAANPVYHERTKHIEVDCHSIREAYDRRI 991
E +WL L +L + ++ DN SA+ +AAN V+H+RT++IE+DCH + I
Sbjct: 21 EALWLTYALADLRIASLLLVVIYCDNRSALHLAANSVFHKRTENIEIDCHIV*VKVLFGI 200
Query: 992 INLPHVSTSVQTADIFTKSLTR 1013
++L HV +S Q AD+FTK++++
Sbjct: 201 LHLLHVPSSDQVADVFTKTISQ 266
>TC12832 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (9%)
Length = 747
Score = 65.5 bits (158), Expect = 4e-11
Identities = 39/147 (26%), Positives = 76/147 (51%), Gaps = 9/147 (6%)
Frame = +2
Query: 682 FRSTLLGFEFSQSRYDPSLFLQR-TPKGMVVLLVYVDDIVVTGSDQDAISRIKNLLHSTF 740
F S ++ +++ D + +R ++LL+YVDD++V G ++D + +K L F
Sbjct: 2 FDSFIMSLGYNRLSSDHCTYHKRFDDNDFIILLLYVDDMLVVGPNKDRVQELKAQLAREF 181
Query: 741 HMKELGRLTYFLGLEVHYHHEG--VFLNQQKYIQDLVQLAGLTNATLVDTPMEVNVKYR- 797
MK+LG LG+++H + ++L+Q+ Y+Q +++ + + + TP+ VN K
Sbjct: 182 DMKDLGPANKILGMQIHRDRKDRRIWLSQKNYLQKVLRRFNMQDCNPISTPLPVNYKLSS 361
Query: 798 ----RDEGDHLD-DPTQYRKLVGSLIY 819
E + ++ Y VGSL+Y
Sbjct: 362 SMIPSSEAERMEMSRVPYASAVGSLMY 442
>TC17418 similar to UP|Q94AX2 (Q94AX2) AT5g39790/MKM21_80, partial (20%)
Length = 739
Score = 57.0 bits (136), Expect = 1e-08
Identities = 43/141 (30%), Positives = 66/141 (46%), Gaps = 1/141 (0%)
Frame = -1
Query: 767 QQKYIQDLVQLAGLTNATLVDTPME-VNVKYRRDEGDHLDDPTQYRKLVGSLIYVTITRP 825
Q+ Y+ D+++ +TN+ + T + ++ R G D T Y+ L+GS+ Y+ R
Sbjct: 739 QKIYVDDILKKFKMTNSKYISTTIGGKEIEAGRRNGGKRVDSTYYKSLIGSVRYLNTVRS 560
Query: 826 DISFAVHTVSKFMQAPRHFHLSAVQQIIRYLLGTLKRGLFFPVGSSIKLQAYSDADWAGC 885
DI V S+FM+ P H Q+ +RY+ GTLK G+F V I + D
Sbjct: 559 DIVCGVGLRSRFME-P*DCH*QGAQRSLRYIKGTLKDGIFMLV---ITM*NSLDTQIVTR 392
Query: 886 PDTRKSTTGWCMFLGNAPISW 906
P G+ LG ISW
Sbjct: 391 P-------GYAFHLGRGAISW 350
>BP057233
Length = 473
Score = 55.5 bits (132), Expect = 4e-08
Identities = 29/67 (43%), Positives = 41/67 (60%)
Frame = -2
Query: 953 LHADNTSAIQIAANPVYHERTKHIEVDCHSIREAYDRRIINLPHVSTSVQTADIFTKSLT 1012
L DN SA +A+NPV H R+KHIE+D H IR+ + + + +V T+ Q AD TK L+
Sbjct: 445 LWCDNLSAKALASNPVLHARSKHIEIDVHYIRDQVLQNEVVVAYVPTTDQIADCLTKPLS 266
Query: 1013 RQRHNFL 1019
R + L
Sbjct: 265 HTRFSQL 245
>AV766665
Length = 601
Score = 49.3 bits (116), Expect = 3e-06
Identities = 26/75 (34%), Positives = 41/75 (54%)
Frame = +3
Query: 851 QIIRYLLGTLKRGLFFPVGSSIKLQAYSDADWAGCPDTRKSTTGWCMFLGNAPISWKCKK 910
+I YL +RG F + ++ AD+ G R ST G+ MFL ++W+ K+
Sbjct: 372 RIF*YLKANSRRGPLFQKEGKSSMDGFTYADYLGSIVDRLSTMGYYMFLSGNLVTWRSKQ 551
Query: 911 QDSVSKSSTEAEYRA 925
Q+ +++SS EAE RA
Sbjct: 552 QNIIARSSGEAELRA 596
>TC10484 similar to UP|Q850H7 (Q850H7) Gag-pol polyprotein (Fragment),
partial (20%)
Length = 479
Score = 34.7 bits (78), Expect(2) = 3e-06
Identities = 13/33 (39%), Positives = 22/33 (66%)
Frame = +3
Query: 935 WLRGLLTELGFSQDQPTPLHADNTSAIQIAANP 967
W+ +L+E+G + P PL DN +A+ I++NP
Sbjct: 3 WVGQILSEMGIERISPMPLWCDNQAALHISSNP 101
Score = 33.9 bits (76), Expect(2) = 3e-06
Identities = 13/13 (100%), Positives = 13/13 (100%)
Frame = +2
Query: 970 HERTKHIEVDCHS 982
HERTKHIEVDCHS
Sbjct: 113 HERTKHIEVDCHS 151
>BP084259
Length = 385
Score = 48.5 bits (114), Expect = 5e-06
Identities = 20/58 (34%), Positives = 34/58 (58%)
Frame = -3
Query: 924 RAMSAACSEIIWLRGLLTELGFSQDQPTPLHADNTSAIQIAANPVYHERTKHIEVDCH 981
R + + +E +WL+ LL EL FS + + ++N +A + N V+H KH+ +DCH
Sbjct: 179 RTIGSTAAEFLWLQQLLKELQFSLPKVPCIFSNNINATYVCLNLVFHSHMKHVALDCH 6
>TC19389 weakly similar to UP|POLX_TOBAC (P10978) Retrovirus-related Pol
polyprotein from transposon TNT 1-94 [Contains: Protease
; Reverse transcriptase ; Endonuclease] , partial (6%)
Length = 498
Score = 47.4 bits (111), Expect = 1e-05
Identities = 28/118 (23%), Positives = 55/118 (45%)
Frame = +3
Query: 898 FLGNAPISWKCKKQDSVSKSSTEAEYRAMSAACSEIIWLRGLLTELGFSQDQPTPLHADN 957
F G A ++W + Q V+ S+ EAE+ A + AC E++W++ L F
Sbjct: 12 FAGGA-VAWPSRLQKCVALSTAEAEFIAATEACHELLWMKNFLQNAWFHSHPILCCIVIT 188
Query: 958 TSAIQIAANPVYHERTKHIEVDCHSIREAYDRRIINLPHVSTSVQTADIFTKSLTRQR 1015
+ +A ++ + + +R+ + +++ L + T AD+ TKSL R++
Sbjct: 189 KALFTLARILLFIQDPSTLMFVIIGLRDVLNSKLLELEKIHTDDDGADMMTKSLPREK 362
>AV769206
Length = 536
Score = 47.0 bits (110), Expect = 2e-05
Identities = 22/55 (40%), Positives = 35/55 (63%)
Frame = -3
Query: 384 LPPQERTKFTAQSVECAFLGYSPHQKGFLCYDPNLRRIRVSRNVIFQENKYFFAS 438
L P TK + S EC F+GYS + KG+ C D + RI +S++V+F E+++ + S
Sbjct: 531 LRPYNSTKLSLHSKECVFIGYSINHKGYKCLDQS-GRIYISKDVLFHEHRFPYPS 370
>AV767042
Length = 444
Score = 46.2 bits (108), Expect = 3e-05
Identities = 18/44 (40%), Positives = 32/44 (71%)
Frame = -3
Query: 594 QEYGLDYDETFAPVAKMTTVRTILAIAASQAWPLHQMDVKNAFL 637
Q G+D ++TF+PV K +RT+ IA S++WP+ Q++V+N ++
Sbjct: 349 QTAGVDCNKTFSPVVKPAPIRTVFTIALSRSWPIPQLNVQNLWI 218
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.339 0.147 0.496
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 20,920,961
Number of Sequences: 28460
Number of extensions: 356247
Number of successful extensions: 2658
Number of sequences better than 10.0: 64
Number of HSP's better than 10.0 without gapping: 2594
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2647
length of query: 1019
length of database: 4,897,600
effective HSP length: 99
effective length of query: 920
effective length of database: 2,080,060
effective search space: 1913655200
effective search space used: 1913655200
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.8 bits)
S2: 60 (27.7 bits)
Medicago: description of AC146527.6