BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146343.8 + phase: 0 /pseudo
(379 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC14863 200 3e-52
TC14765 homologue to UP|Q8T5T7 (Q8T5T7) Porin, partial (4%) 29 1.5
TC19380 similar to UP|Q9LSV9 (Q9LSV9) Similarity to transporter ... 29 1.5
TC14595 homologue to UP|Q9FGU1 (Q9FGU1) Gb|AAD34459.1 (SPP30-lik... 28 2.5
TC8117 similar to UP|Q9XGN6 (Q9XGN6) ESTs C99174(E10437), partia... 28 3.3
AV416594 27 4.3
TC14126 similar to UP|Q84UB0 (Q84UB0) Transcription factor Myb1,... 27 4.3
TC12836 GB|CAA71302.1|2292921|LJPANC pantoate--beta-alanine liga... 27 4.3
TC14529 similar to UP|Q8LAQ0 (Q8LAQ0) Membrane related protein-l... 27 5.6
CB827522 27 5.6
BP036137 27 5.6
TC19637 similar to UP|Q9T0D0 (Q9T0D0) T5C23.60 protein, partial ... 27 7.3
TC17088 weakly similar to UP|O82699 (O82699) MADS-box protein, p... 26 9.6
>TC14863
Length = 1803
Score = 200 bits (509), Expect = 3e-52
Identities = 119/327 (36%), Positives = 182/327 (55%), Gaps = 7/327 (2%)
Frame = +3
Query: 26 LRTRSSFSLQRVSKGCSISS------FACSTSKLHGGRVGFNHREGKTSFLKFGVDESVV 79
L R SL + C+I S FA + S L V + K D S
Sbjct: 270 LDVRGGVSLHENGRSCAIRSELRGPVFAAARSPL----VNLKPKRNSQILCKATTDLSGD 437
Query: 80 GGGGNGDVGERDWS-QILSALLPFVVAVTAVAALSQPSTFTWVSKELYAPALGGIMLSIG 138
G +G++ + + + L+ L P V + A+ + +PS TW+ +L+ LG +MLS+G
Sbjct: 438 VPGSSGELSQYEKVIETLTTLFPLWVILGALVGIYKPSAVTWLETDLFTLGLGFLMLSMG 617
Query: 139 IGLSMEDFALAFKRPLPLSIGFIAQYVLKPVLGVLIAKAFGLPRMFYAGFVLTACVSGAQ 198
+ L+ EDF + P + +GF+AQY++KPVLG I+ L G +L +C G Q
Sbjct: 618 LTLTFEDFRRCMRNPWTVGVGFLAQYLIKPVLGFFISLTLKLSAPLATGLILVSCCPGGQ 797
Query: 199 LSSYASFISKGDVALCIVLTSYTTIASVIVTPLLTGLLIGSVVPVDAVAMSKSILQVVLA 258
S+ A++ISKG+VAL +++T+ +TI ++I+TPLLT LL G +VPVDA ++ S QVVL
Sbjct: 798 ASNVATYISKGNVALSVLMTTCSTIGAIIMTPLLTKLLAGQLVPVDAAGLAISTFQVVLV 977
Query: 259 PVTLGLLLNTYAKPVVSILRPVMPFVAMICTSLCIGSPLAINRSQILSGEGLRLVAPVLI 318
P +G+LLN + S + V P + +I T+L SP+ +++L +G +L+ PV+
Sbjct: 978 PTIVGVLLNEFFPKFTSKIITVTPLIGVILTTLLCASPIG-QVAEVLKTQGAQLILPVMA 1154
Query: 319 FHAAAFTLGYWFSNLPSLRFLTSITIN 345
HAAAF +GYW S L S TS TI+
Sbjct: 1155LHAAAFAIGYWMSKL-SFGESTSRTIS 1232
>TC14765 homologue to UP|Q8T5T7 (Q8T5T7) Porin, partial (4%)
Length = 1114
Score = 28.9 bits (63), Expect = 1.5
Identities = 20/52 (38%), Positives = 22/52 (41%)
Frame = -1
Query: 289 TSLCIGSPLAINRSQILSGEGLRLVAPVLIFHAAAFTLGYWFSNLPSLRFLT 340
T C S NRS + E P + F AAA G W S P LR LT
Sbjct: 412 TCFCTVSGSGPNRSVVPKTESK---PPSIFFFAAAAVAGSWLSFSPLLRILT 266
>TC19380 similar to UP|Q9LSV9 (Q9LSV9) Similarity to transporter protein,
partial (6%)
Length = 519
Score = 28.9 bits (63), Expect = 1.5
Identities = 11/15 (73%), Positives = 12/15 (79%)
Frame = +3
Query: 365 AELHTCRPSSNTIFG 379
AELH CRP+ N IFG
Sbjct: 3 AELHACRPTCNPIFG 47
>TC14595 homologue to UP|Q9FGU1 (Q9FGU1) Gb|AAD34459.1 (SPP30-like protein)
(At5g58030), complete
Length = 1093
Score = 28.1 bits (61), Expect = 2.5
Identities = 18/45 (40%), Positives = 24/45 (53%), Gaps = 2/45 (4%)
Frame = -3
Query: 1 MASIPVFSPFTPMKQKHKPLSF--QPLLRTRSSFSLQRVSKGCSI 43
++ P P T Q + P+SF + L TRSSFSL S CS+
Sbjct: 644 LSKTPRTIPATNAPQLNVPMSFGIEMNLFTRSSFSLIMYSSSCSV 510
>TC8117 similar to UP|Q9XGN6 (Q9XGN6) ESTs C99174(E10437), partial (41%)
Length = 766
Score = 27.7 bits (60), Expect = 3.3
Identities = 22/57 (38%), Positives = 26/57 (45%)
Frame = +2
Query: 11 TPMKQKHKPLSFQPLLRTRSSFSLQRVSKGCSISSFACSTSKLHGGRVGFNHREGKT 67
TP L F LLR+ S SL S CS SS CS + R+ +EGKT
Sbjct: 50 TPFSGLRLGLGFSHLLRSLSFRSLSN*SFSCSESSVRCSIIPI---RIYGFPQEGKT 211
>AV416594
Length = 393
Score = 27.3 bits (59), Expect = 4.3
Identities = 33/118 (27%), Positives = 52/118 (43%), Gaps = 7/118 (5%)
Frame = +2
Query: 14 KQKHKPLSFQPLLRTRSSFSLQRVSKGCSIS-SF-----ACSTSKL-HGGRVGFNHREGK 66
K +H PL+ P RTR + QR+S C + SF AC T ++ GR+ R G+
Sbjct: 95 KSRHLPLA--PSTRTRWNVKKQRISGRCLLQPSFGALPHACRTVRVRRSGRM--EVRSGE 262
Query: 67 TSFLKFGVDESVVGGGGNGDVGERDWSQILSALLPFVVAVTAVAALSQPSTFTWVSKE 124
T ++ G++G+R + + L A+ A P T WVS++
Sbjct: 263 TCWVT------------QGNIGQRPVLRHVCLL-----AMLGEAPCCCPGTERWVSRD 385
>TC14126 similar to UP|Q84UB0 (Q84UB0) Transcription factor Myb1, partial
(44%)
Length = 1500
Score = 27.3 bits (59), Expect = 4.3
Identities = 15/60 (25%), Positives = 29/60 (48%)
Frame = -3
Query: 259 PVTLGLLLNTYAKPVVSILRPVMPFVAMICTSLCIGSPLAINRSQILSGEGLRLVAPVLI 318
P+T L ++ + +V LRP P +A+ L + ++ G+ + +VA V+I
Sbjct: 433 PITFPYFLQSHQEQLVLFLRPRYPSLALAVALLTGAGRSGVVGGGVIGGDNVFVVAVVVI 254
>TC12836 GB|CAA71302.1|2292921|LJPANC pantoate--beta-alanine ligase {Lotus
corniculatus var. japonicus;} , complete
Length = 1310
Score = 27.3 bits (59), Expect = 4.3
Identities = 32/122 (26%), Positives = 55/122 (44%), Gaps = 8/122 (6%)
Frame = +3
Query: 206 ISKGDVALCIVLTSYTTIASVIVTPLLTGLLIGSVVPVDAVAMSKSILQVVLAPVTLGLL 265
ISK +VL ++T ++T ++ +L SVV + ++ V+ + LGL
Sbjct: 258 ISKNSCLFLVVLMLFSTPTICMITVVMAVMLWRSVV---VMGWCLVLIGGVVLGMKLGLE 428
Query: 266 LNTYAKPVVSI--------LRPVMPFVAMICTSLCIGSPLAINRSQILSGEGLRLVAPVL 317
L ++ P V L P++P +I SL + L + R I +G+ R +L
Sbjct: 429 LRSWRNPFVGRVGLFSLEGLPPLLP-SCLILWSLML---LCLGRRTISNGKLFREWFEIL 596
Query: 318 IF 319
IF
Sbjct: 597 IF 602
>TC14529 similar to UP|Q8LAQ0 (Q8LAQ0) Membrane related protein-like,
partial (49%)
Length = 1212
Score = 26.9 bits (58), Expect = 5.6
Identities = 18/39 (46%), Positives = 22/39 (56%), Gaps = 1/39 (2%)
Frame = +2
Query: 19 PLSFQPLLRTRS-SFSLQRVSKGCSISSFACSTSKLHGG 56
P S PL S +F +R+ GCS SS A ST+ HGG
Sbjct: 2 PHSSPPLFIALSPNFPNRRI--GCSASSSAASTTATHGG 112
>CB827522
Length = 519
Score = 26.9 bits (58), Expect = 5.6
Identities = 11/22 (50%), Positives = 14/22 (63%)
Frame = -1
Query: 5 PVFSPFTPMKQKHKPLSFQPLL 26
P FS + P+ QKH L F+P L
Sbjct: 501 PSFSSWCPLDQKHHVLLFEPFL 436
>BP036137
Length = 279
Score = 26.9 bits (58), Expect = 5.6
Identities = 14/30 (46%), Positives = 17/30 (56%)
Frame = -3
Query: 24 PLLRTRSSFSLQRVSKGCSISSFACSTSKL 53
P LR RSS S +S GC+ S + ST L
Sbjct: 241 PALRRRSSSSFSELSIGCAAVSASISTRSL 152
>TC19637 similar to UP|Q9T0D0 (Q9T0D0) T5C23.60 protein, partial (48%)
Length = 603
Score = 26.6 bits (57), Expect = 7.3
Identities = 24/90 (26%), Positives = 41/90 (44%), Gaps = 3/90 (3%)
Frame = -2
Query: 150 FKRPLPLSIGFIAQYVLKPVLGVLIAKAFGL-PRMFYAGFVLTACVSGAQLSSYASFISK 208
F P P +I I L+P L + I F + R+ ++G +C + FIS
Sbjct: 572 FSMPTPATILLILNVELRPALRLAITMPFIIETRLLFSGTSTLSC------TV*PGFIS- 414
Query: 209 GDVALCIVLTSYTTIASV--IVTPLLTGLL 236
+ LC +S +++ + I P+L+ LL
Sbjct: 413 -GICLCAFTSSTASLSKICMICLPVLSNLL 327
>TC17088 weakly similar to UP|O82699 (O82699) MADS-box protein, partial
(38%)
Length = 578
Score = 26.2 bits (56), Expect = 9.6
Identities = 14/54 (25%), Positives = 23/54 (41%)
Frame = +3
Query: 9 PFTPMKQKHKPLSFQPLLRTRSSFSLQRVSKGCSISSFACSTSKLHGGRVGFNH 62
PF P + +P L+ +Q S +S C T+ +HG + F+H
Sbjct: 177 PFHPAQSNPMDCQPEPFLQIGYHQYVQAESSSIPKNSMTCETNFMHGWML*FDH 338
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.324 0.138 0.409
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,434,104
Number of Sequences: 28460
Number of extensions: 90093
Number of successful extensions: 719
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 711
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 718
length of query: 379
length of database: 4,897,600
effective HSP length: 92
effective length of query: 287
effective length of database: 2,279,280
effective search space: 654153360
effective search space used: 654153360
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 56 (26.2 bits)
Medicago: description of AC146343.8