BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146341.6 + phase: 0
(540 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC18643 similar to UP|Q945B9 (Q945B9) Growth-on protein GRO11, p... 280 5e-76
AV773627 186 1e-47
TC16251 similar to UP|Q945B9 (Q945B9) Growth-on protein GRO11, p... 164 3e-41
TC15099 weakly similar to UP|Q8LQG1 (Q8LQG1) Vacuolar sorting re... 105 2e-23
TC15446 similar to UP|AAR98738 (AAR98738) Signal peptide peptida... 61 4e-10
BP045762 49 3e-06
TC17440 similar to UP|SP25_ARATH (P58684) Probable microsomal si... 28 3.8
TC7873 homologue to UP|CHSY_PUELO (P23569) Chalcone synthase (N... 28 4.9
TC17229 similar to UP|O49595 (O49595) HMG protein (AT3G51880/ORF... 27 6.4
TC14395 weakly similar to UP|C823_SOYBN (O49858) Cytochrome P450... 27 8.4
AV425937 27 8.4
CB827912 27 8.4
>TC18643 similar to UP|Q945B9 (Q945B9) Growth-on protein GRO11, partial
(33%)
Length = 846
Score = 280 bits (715), Expect = 5e-76
Identities = 132/152 (86%), Positives = 140/152 (91%)
Frame = +3
Query: 300 CFRWFQYPAQTYVKIPFFGAVPYLTLAVTPFCIVFAVVWAVKRQASYAWIGQDILGIALI 359
CFRWFQ+ QT+VK PFFGAV YLTLAVTPFCIVF+V+W V S+AWIGQDILGIALI
Sbjct: 3 CFRWFQHAGQTFVKAPFFGAVSYLTLAVTPFCIVFSVLWGVFHHESFAWIGQDILGIALI 182
Query: 360 ITVLQIVRIPNLKVGTVLLSCAFLYDILWVFVSKWWFHESVMIVVARGDKSGEDGIPMLL 419
ITVLQIV IPNLKVGTVLLSCAFLYDI WVFVSKWWFHESVMIVVARGDKS EDGIPMLL
Sbjct: 183 ITVLQIVHIPNLKVGTVLLSCAFLYDIFWVFVSKWWFHESVMIVVARGDKSREDGIPMLL 362
Query: 420 KLPRLFDPWGGYSIIGFGDIILPGLVVAFSLR 451
K+PRLFDPWGGYS+IGFGDIILPGL+VAFSLR
Sbjct: 363 KIPRLFDPWGGYSVIGFGDIILPGLLVAFSLR 458
Score = 57.0 bits (136), Expect = 8e-09
Identities = 24/30 (80%), Positives = 28/30 (93%)
Frame = +2
Query: 451 RYDWLAKKNLRAGYFVWAMTAYGLGLLITY 480
RYDWLAKKNLRAGYF++AMTAYGLG+L +
Sbjct: 638 RYDWLAKKNLRAGYFLYAMTAYGLGMLCLF 727
>AV773627
Length = 465
Score = 186 bits (471), Expect = 1e-47
Identities = 89/120 (74%), Positives = 103/120 (85%)
Frame = +2
Query: 6 VIYSNIVAYVFLFSVSLVLAGDIVHHDDVAPTRPGCENNFVLVKVPTWIDGVENAEYVGV 65
V++ +V VF+ V+L AGDIVH D+VAP RPGC+NNFVLV VPTW+DGV + EYVGV
Sbjct: 104 VLFWLVVCSVFMVLVTLSWAGDIVHPDNVAPKRPGCDNNFVLVTVPTWMDGVVSCEYVGV 283
Query: 66 GARFGPTLESKEKHANHTRVVMADPPDCCSKPKNKLTNEIILVHRGKCSFTTKANIADEA 125
GARFGP+LESKEK ANHTRV +ADPPD CSKPKNKLT EIILV+RG+CSFTTKANIA+EA
Sbjct: 284 GARFGPSLESKEKRANHTRVAIADPPDGCSKPKNKLTGEIILVYRGQCSFTTKANIAEEA 463
>TC16251 similar to UP|Q945B9 (Q945B9) Growth-on protein GRO11, partial (9%)
Length = 905
Score = 164 bits (416), Expect = 3e-41
Identities = 90/228 (39%), Positives = 132/228 (57%), Gaps = 2/228 (0%)
Frame = +3
Query: 36 PTRPGCENNFVLVKVPTWIDGVENAEYVGVGARFGPTLESKEKHANHTRVVMADPPDCCS 95
P C N F LVKV W+DG E Y GV ARFG L +++ T + ++P DCCS
Sbjct: 99 PKSKPCNNPFRLVKVNNWVDGEEANTYDGVTARFGSLLPENPENSVKTPAIFSNPVDCCS 278
Query: 96 KPKNKLTNEIILVHRGKCSFTTKANIADEAGASAILIINYRTELFKMVCEENETDVDIGI 155
++L++ + L RG C FTTKA A GASA+L+IN +LF+M C N T +I I
Sbjct: 279 TSTSQLSDSVALCTRGGCDFTTKAAFAQSGGASAMLLINDEEDLFEMAC-SNSTGGNISI 455
Query: 156 PAVMLPQDAGLNLERHIQNNSIVSIQLYSPLRPLVDVAEVFLWLMAVGTILCASYWSAWT 215
P V++ + AG + + + V + LY+P PLVD + FLWLMAVGTI+CAS WS T
Sbjct: 456 PVVLITKSAGEAFNKSLASGRKVEVLLYAPPSPLVDFSFAFLWLMAVGTIVCASLWSDIT 635
Query: 216 AREAAIE--QEKLLKDASDEYVAESVGSRGYVEISTTAAILFVVLASC 261
+ + E E K++S+ A+ V + + I++ +A++F+ + C
Sbjct: 636 TPKKSGEHYNELFPKESSNVEGAKDVSDKEILNINSMSAVVFIHINIC 779
Score = 35.0 bits (79), Expect = 0.031
Identities = 17/46 (36%), Positives = 27/46 (57%)
Frame = +2
Query: 252 AILFVVLASCFLVMLYKLMSFWFLEVLVVLFCIGGIEGLQTCLTAL 297
++L +SC L +++ VLVVLFCI G+EG+ C+ +L
Sbjct: 779 SLLIYYSSSCHLGLIW---------VLVVLFCIAGVEGMHNCIISL 889
>TC15099 weakly similar to UP|Q8LQG1 (Q8LQG1) Vacuolar sorting receptor-like
protein, partial (16%)
Length = 687
Score = 105 bits (262), Expect = 2e-23
Identities = 47/76 (61%), Positives = 60/76 (78%)
Frame = +1
Query: 428 WGGYSIIGFGDIILPGLVVAFSLRYDWLAKKNLRAGYFVWAMTAYGLGLLITYVALNLMD 487
WGGY +IGFGDII PGL+ +F+ R+D KK GYF+W + YG+GL++TY+AL LMD
Sbjct: 16 WGGYDMIGFGDIIFPGLLTSFAHRFDKDNKKGALNGYFLWTVLGYGVGLVLTYLALYLMD 195
Query: 488 GHGQPALLYIVPFTLG 503
G+GQPALLY+VP TLG
Sbjct: 196 GNGQPALLYLVPCTLG 243
>TC15446 similar to UP|AAR98738 (AAR98738) Signal peptide peptidase
(Fragment), partial (75%)
Length = 697
Score = 61.2 bits (147), Expect = 4e-10
Identities = 30/73 (41%), Positives = 46/73 (62%)
Frame = +1
Query: 431 YSIIGFGDIILPGLVVAFSLRYDWLAKKNLRAGYFVWAMTAYGLGLLITYVALNLMDGHG 490
+S++G GDI++PG+ VA +LR+D + + YF A Y +G+++T V +N
Sbjct: 34 FSMLGLGDIVIPGIFVALALRFD--VSRGKQPQYFKSAFLGYAVGVILTIVVMNWFQA-A 204
Query: 491 QPALLYIVPFTLG 503
QPALLYIVP +G
Sbjct: 205 QPALLYIVPSVIG 243
>BP045762
Length = 562
Score = 48.5 bits (114), Expect = 3e-06
Identities = 26/83 (31%), Positives = 48/83 (57%), Gaps = 10/83 (12%)
Frame = -1
Query: 431 YSIIGFGDIILPGLVVAFSLRYDWLAKKNL---------RAGYFVW-AMTAYGLGLLITY 480
+ ++G GD+ +PG+++A L +D+ ++ + ++W A+ Y +GL +T
Sbjct: 493 FMMLGLGDMAIPGMLLALVLCFDYRKSRDTVNLFELYSSKGHKYIWYALPGYAIGL-VTA 317
Query: 481 VALNLMDGHGQPALLYIVPFTLG 503
+A ++ QPALLY+VP TLG
Sbjct: 316 LAAGVLTHSPQPALLYLVPSTLG 248
>TC17440 similar to UP|SP25_ARATH (P58684) Probable microsomal signal
peptidase 25 kDa subunit (SPase 25 kDa subunit) (SPC25)
, partial (67%)
Length = 503
Score = 28.1 bits (61), Expect = 3.8
Identities = 21/70 (30%), Positives = 32/70 (45%), Gaps = 11/70 (15%)
Frame = +1
Query: 209 SYWSAWTAREAAIEQEKLLKDAS-----------DEYVAESVGSRGYVEISTTAAILFVV 257
S WS+ + R + K LK A+ DE V+E V SRGYVE + + +V
Sbjct: 49 SIWSSSSLRLRVLMASKSLKKANLLDHNSIKHILDESVSEIVTSRGYVEDVRLSNVRLLV 228
Query: 258 LASCFLVMLY 267
++ L+
Sbjct: 229 GTVIIVIALF 258
>TC7873 homologue to UP|CHSY_PUELO (P23569) Chalcone synthase
(Naringenin-chalcone synthase) , partial (35%)
Length = 534
Score = 27.7 bits (60), Expect = 4.9
Identities = 11/45 (24%), Positives = 25/45 (55%)
Frame = +1
Query: 378 LSCAFLYDILWVFVSKWWFHESVMIVVARGDKSGEDGIPMLLKLP 422
L+CA ++ +LW+ WW + + V + + ++G+ + +LP
Sbjct: 364 LTCALIWHLLWMLGKTWWL*KYLDXVKKQQ*RLLKNGVNLSPRLP 498
>TC17229 similar to UP|O49595 (O49595) HMG protein (AT3G51880/ORF13),
partial (66%)
Length = 540
Score = 27.3 bits (59), Expect = 6.4
Identities = 14/39 (35%), Positives = 22/39 (55%), Gaps = 3/39 (7%)
Frame = -2
Query: 271 SFWFLEVLVVLFCI--GGIEGLQTCLT-ALLSCFRWFQY 306
SFWF+ + + L C+ + G + L+ + LS WFQY
Sbjct: 272 SFWFVGIFLGLLCLPCSTLIGFNSRLSLSYLSVINWFQY 156
>TC14395 weakly similar to UP|C823_SOYBN (O49858) Cytochrome P450 82A3
(P450 CP6) , partial (38%)
Length = 1079
Score = 26.9 bits (58), Expect = 8.4
Identities = 10/33 (30%), Positives = 20/33 (60%)
Frame = +3
Query: 255 FVVLASCFLVMLYKLMSFWFLEVLVVLFCIGGI 287
F++L CFL++ ++ + F V L+C+ G+
Sbjct: 852 FMLLLYCFLLLFFRPVVFRCFNSFVFLYCVLGV 950
>AV425937
Length = 429
Score = 26.9 bits (58), Expect = 8.4
Identities = 17/45 (37%), Positives = 27/45 (59%)
Frame = +2
Query: 91 PDCCSKPKNKLTNEIILVHRGKCSFTTKANIADEAGASAILIINY 135
P C S PKNK+ NE+++V C + + + E GA A+L +N+
Sbjct: 269 PSCHSPPKNKV-NELLVV---SCGLSLQPLL--ETGAIAMLGVNH 385
>CB827912
Length = 348
Score = 26.9 bits (58), Expect = 8.4
Identities = 12/25 (48%), Positives = 14/25 (56%)
Frame = +1
Query: 270 MSFWFLEVLVVLFCIGGIEGLQTCL 294
M W ++L VLFC G G Q CL
Sbjct: 10 MRDWVKKLLPVLFCKRGSRGAQVCL 84
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.327 0.142 0.447
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,582,528
Number of Sequences: 28460
Number of extensions: 159216
Number of successful extensions: 1014
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 1000
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1011
length of query: 540
length of database: 4,897,600
effective HSP length: 95
effective length of query: 445
effective length of database: 2,193,900
effective search space: 976285500
effective search space used: 976285500
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 57 (26.6 bits)
Medicago: description of AC146341.6