BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146333.2 - phase: 0
(615 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BE122516 92 2e-19
TC18927 similar to PIR|AI2934|AI2934 chromate transport protein ... 87 1e-17
TC11885 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, parti... 53 1e-07
BI418821 52 4e-07
AU251673 51 6e-07
TC9521 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, part... 41 6e-04
TC10011 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, par... 40 8e-04
TC13053 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, parti... 40 0.001
BP039068 32 0.39
TC17929 32 0.39
TC20066 similar to GB|AAM91455.1|22137220|AY133625 AT3g11400/F24... 32 0.39
BP043432 30 1.1
TC9283 29 2.5
TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, ... 28 3.3
TC16357 weakly similar to UP|Q8DZP0 (Q8DZP0) Bacterial luciferas... 28 5.6
TC9401 similar to UP|Q84LW7 (Q84LW7) Peptidylprolyl cis-trans is... 28 5.6
AV773507 28 5.6
TC8573 similar to UP|Q9SIH5 (Q9SIH5) At2g36090 protein, partial ... 27 7.4
TC16235 27 9.6
>BE122516
Length = 364
Score = 92.4 bits (228), Expect = 2e-19
Identities = 48/115 (41%), Positives = 73/115 (62%)
Frame = +2
Query: 424 LGMNWLEYNHVLINCFSKSVHFSSVEEESGAEFLSTKQLKQLERDGILMFSLMATLSIEN 483
+GMNWL N +NC K+V F + E ++ + K K E + ++ + T ++
Sbjct: 14 VGMNWLTANDATLNCRKKTVTFGTSEGDAKRVKRTDKVGKASECESDVLLGALET--DKS 187
Query: 484 QAVIDRLPVVCDFPEVFPDEIPDVPPEREVEFSIDLVPGTKPVSMAPYRISASEL 538
++ +PVV +F +VFP+E+ ++PPEREVEFSID VPGT P+S+APYR+S EL
Sbjct: 188 DTGVEGIPVVREFSDVFPEEVSELPPEREVEFSID*VPGTGPISIAPYRMSLVEL 352
>TC18927 similar to PIR|AI2934|AI2934 chromate transport protein chrA
[imported] - Agrobacterium tumefaciens
(strain C58, Dupont) {Agrobacterium tumefaciens;},
partial (6%)
Length = 561
Score = 86.7 bits (213), Expect = 1e-17
Identities = 48/155 (30%), Positives = 72/155 (45%), Gaps = 7/155 (4%)
Frame = -2
Query: 218 RKGKGQQSRPKPYSAPADKGKQKMVD------VRRPKKKDAAEIVCFNCGEKGHKSNACP 271
R + + + KP+ P ++G V RP + D +EIVC C +KGH +N CP
Sbjct: 464 RFDRNKSFQKKPFQRPQNRGTSSGYSHSFGNFVPRPTQSDTSEIVCHRCSKKGHFANRCP 285
Query: 272 EEIKKCVRCGKKGHVVADCNRTDI-VCFNCNGEGHISSQCTQPKRAPTTGRVFALTGTQT 330
+ + C C K GH DC + N + + KR + RV+ ++G ++
Sbjct: 284 DLV--CWNCQKTGHSGKDCTNPKVEAATNAIAARRPAPAANKGKRPVASARVYTVSGAES 111
Query: 331 ENEDRLIRGTCYINNTPLVAIIDTGATHCFIAFDC 365
D LIR +N PL + D+GATH FI C
Sbjct: 110 HRADGLIRSVGSVNCKPLTILFDSGATHSFIDLAC 6
>TC11885 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, partial (26%)
Length = 555
Score = 53.1 bits (126), Expect = 1e-07
Identities = 25/93 (26%), Positives = 45/93 (47%), Gaps = 1/93 (1%)
Frame = +2
Query: 219 KGKGQQSRPKPYSAPADKGKQKMVDVRRPKKKD-AAEIVCFNCGEKGHKSNACPEEIKKC 277
+ + + P +D+ + RR ++ + + +C NC GH + CP + C
Sbjct: 227 RSRSRSRSPMDRKIRSDRFSYRDAPYRRDSRRGFSRDNLCKNCKRPGHFARECPN-VAIC 403
Query: 278 VRCGKKGHVVADCNRTDIVCFNCNGEGHISSQC 310
CG GH+ ++C T +C+NC GH++S C
Sbjct: 404 HNCGLPGHIASECT-TKSLCWNCKEPGHMASSC 499
Score = 36.6 bits (83), Expect = 0.012
Identities = 15/35 (42%), Positives = 19/35 (53%)
Frame = +2
Query: 277 CVRCGKKGHVVADCNRTDIVCFNCNGEGHISSQCT 311
C C + GH +C I C NC GHI+S+CT
Sbjct: 344 CKNCKRPGHFARECPNVAI-CHNCGLPGHIASECT 445
Score = 28.5 bits (62), Expect = 3.3
Identities = 8/18 (44%), Positives = 14/18 (77%)
Frame = +2
Query: 256 VCFNCGEKGHKSNACPEE 273
+C+NC E GH +++CP +
Sbjct: 455 LCWNCKEPGHMASSCPND 508
>BI418821
Length = 614
Score = 51.6 bits (122), Expect = 4e-07
Identities = 22/71 (30%), Positives = 32/71 (44%), Gaps = 15/71 (21%)
Frame = +2
Query: 257 CFNCGEKGHKSNACPEEIKK--------CVRCGKKGHVVADCNRTD-------IVCFNCN 301
C+NCG+ GH + C C CG GH+ DCNR++ C+NC
Sbjct: 392 CYNCGDTGHLARDCHRSNNNGGGGGGAACYNCGDAGHLARDCNRSNNNSGGGGAGCYNCG 571
Query: 302 GEGHISSQCTQ 312
GH++ C +
Sbjct: 572 DTGHLARDCNR 604
Score = 40.8 bits (94), Expect = 6e-04
Identities = 16/45 (35%), Positives = 22/45 (48%), Gaps = 7/45 (15%)
Frame = +2
Query: 257 CFNCGEKGHKSNACPEEIKK-------CVRCGKKGHVVADCNRTD 294
C+NCG+ GH + C C CG GH+ DCNR++
Sbjct: 476 CYNCGDAGHLARDCNRSNNNSGGGGAGCYNCGDTGHLARDCNRSN 610
>AU251673
Length = 413
Score = 50.8 bits (120), Expect = 6e-07
Identities = 35/137 (25%), Positives = 64/137 (46%), Gaps = 1/137 (0%)
Frame = +2
Query: 74 TEEQKVRFGTHQLAEEADDWWVALLPTLGQEGAVMTWAVFRREFLRRYFLEDVRGKKEIE 133
++ + V + QL A DW+ L TWA F EF+ R+ + VR +
Sbjct: 8 SDTRAVELASFQLEGVARDWYNVLTRAKPVGSPPWTWADFSAEFMNRFLPQSVRDGFVRD 187
Query: 134 FLELKQG-NMSVTEYAAKFVELSKFYPHYTAENAEFSRCIKFENGLRLDIKRAIGYQQLR 192
F L+Q M+V+EY+A F LS++ P+ E R +F GL+ + +++ +
Sbjct: 188 FERLEQAEGMTVSEYSAHFTHLSRYVPYPLLEE---ERVKRFVRGLKEYLFKSVVGSKSS 358
Query: 193 VFQDLVNSCRIYEEDTK 209
++++ + E+ K
Sbjct: 359 TLSEVLSLALLVEQRQK 409
>TC9521 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, partial (56%)
Length = 598
Score = 40.8 bits (94), Expect = 6e-04
Identities = 16/39 (41%), Positives = 23/39 (58%), Gaps = 2/39 (5%)
Frame = +1
Query: 257 CFNCGEKGHKSNACP--EEIKKCVRCGKKGHVVADCNRT 293
CFNCG GH + C + KC RCG++GH+ +C +
Sbjct: 391 CFNCGIDGHWARDCKAGDWKNKCYRCGERGHIEKNCKNS 507
Score = 35.0 bits (79), Expect = 0.035
Identities = 16/43 (37%), Positives = 19/43 (43%), Gaps = 3/43 (6%)
Frame = +1
Query: 276 KCVRCGKKGHVVADCNRTD--IVCFNCNGEGHISSQC-TQPKR 315
+C CG GH DC D C+ C GHI C PK+
Sbjct: 388 RCFNCGIDGHWARDCKAGDWKNKCYRCGERGHIEKNCKNSPKK 516
Score = 27.7 bits (60), Expect = 5.6
Identities = 10/23 (43%), Positives = 12/23 (51%)
Frame = +1
Query: 257 CFNCGEKGHKSNACPEEIKKCVR 279
C+ CGE+GH C KK R
Sbjct: 457 CYRCGERGHIEKNCKNSPKKLSR 525
>TC10011 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, partial (62%)
Length = 684
Score = 40.4 bits (93), Expect = 8e-04
Identities = 17/39 (43%), Positives = 22/39 (55%), Gaps = 2/39 (5%)
Frame = +2
Query: 257 CFNCGEKGHKSNACP--EEIKKCVRCGKKGHVVADCNRT 293
CFNCG GH + C + KC RCG +GHV +C +
Sbjct: 371 CFNCGLDGHWARDCKAGDWKNKCYRCGDRGHVERNCKNS 487
Score = 37.7 bits (86), Expect = 0.005
Identities = 21/72 (29%), Positives = 29/72 (40%), Gaps = 5/72 (6%)
Frame = +2
Query: 261 GEKGHKSNACPEEIKKCVRCGKKGHVVADCNRTD--IVCFNCNGEGHISSQC-TQPKRA- 316
G + + P +C CG GH DC D C+ C GH+ C PK+
Sbjct: 323 GNREYLGRGPPPGSGRCFNCGLDGHWARDCKAGDWKNKCYRCGDRGHVERNCKNSPKKNE 502
Query: 317 -PTTGRVFALTG 327
T +F+ TG
Sbjct: 503 WQTWKELFSFTG 538
>TC13053 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, partial (9%)
Length = 450
Score = 39.7 bits (91), Expect = 0.001
Identities = 17/49 (34%), Positives = 25/49 (50%), Gaps = 4/49 (8%)
Frame = +3
Query: 277 CVRCGKKGHVVADCNRTDIVCFNCNGEGHISSQCT----QPKRAPTTGR 321
C C + GH+ DC ++C NC G GH++ +C + AP T R
Sbjct: 9 CRNCQQLGHMSRDCMGPLMICHNCGGRGHLAYECPSGRFMDRYAPNTRR 155
Score = 38.5 bits (88), Expect = 0.003
Identities = 14/36 (38%), Positives = 20/36 (54%)
Frame = +3
Query: 255 IVCFNCGEKGHKSNACPEEIKKCVRCGKKGHVVADC 290
+VC NC + GH S C + C CG +GH+ +C
Sbjct: 3 VVCRNCQQLGHMSRDCMGPLMICHNCGGRGHLAYEC 110
Score = 27.3 bits (59), Expect = 7.4
Identities = 8/17 (47%), Positives = 12/17 (70%)
Frame = +3
Query: 255 IVCFNCGEKGHKSNACP 271
++C NCG +GH + CP
Sbjct: 63 MICHNCGGRGHLAYECP 113
>BP039068
Length = 467
Score = 31.6 bits (70), Expect = 0.39
Identities = 15/47 (31%), Positives = 21/47 (43%)
Frame = +3
Query: 254 EIVCFNCGEKGHKSNACPEEIKKCVRCGKKGHVVADCNRTDIVCFNC 300
+ VC NC E GH SN CP + R + + T + C +C
Sbjct: 303 QTVCMNCQETGHASNDCPSLTLRS-RNAQHPRMNQQSGETSVSCSSC 440
>TC17929
Length = 791
Score = 31.6 bits (70), Expect = 0.39
Identities = 10/17 (58%), Positives = 12/17 (69%)
Frame = +2
Query: 257 CFNCGEKGHKSNACPEE 273
C+ CGE GHK CP+E
Sbjct: 62 CYRCGESGHKMRNCPKE 112
>TC20066 similar to GB|AAM91455.1|22137220|AY133625 AT3g11400/F24K9_7
{Arabidopsis thaliana;}, partial (45%)
Length = 557
Score = 31.6 bits (70), Expect = 0.39
Identities = 18/47 (38%), Positives = 25/47 (52%), Gaps = 1/47 (2%)
Frame = +2
Query: 226 RPKPYSAPADKGKQKMVDVRRPKKKDAAEIVCFNCGEKG-HKSNACP 271
RPKP A ++ K P +K A +VC CG+KG H ++ CP
Sbjct: 392 RPKPLGAKTEEPKAS----GDPFQKGAVLMVCRTCGKKGDHWTSRCP 520
>BP043432
Length = 487
Score = 30.0 bits (66), Expect = 1.1
Identities = 14/43 (32%), Positives = 21/43 (48%), Gaps = 4/43 (9%)
Frame = -3
Query: 261 GEKGHKSNACPEEI----KKCVRCGKKGHVVADCNRTDIVCFN 299
G K + N PE++ KK + CG V C+ ++VC N
Sbjct: 350 GHKKKRKNETPEDLERKKKKLIECGTVADAVRTCDICNVVCNN 222
>TC9283
Length = 1307
Score = 28.9 bits (63), Expect = 2.5
Identities = 12/42 (28%), Positives = 24/42 (56%)
Frame = +2
Query: 64 IERIFRVMQYTEEQKVRFGTHQLAEEADDWWVALLPTLGQEG 105
+ER+ R M E+ ++ +G H L+ ++ A++ L +EG
Sbjct: 905 VERLCRAMGGAEKVEIEYGNHSLSNRVEEAVQAIIDFLKREG 1030
>TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, partial
(58%)
Length = 881
Score = 28.5 bits (62), Expect = 3.3
Identities = 9/17 (52%), Positives = 11/17 (63%)
Frame = +2
Query: 257 CFNCGEKGHKSNACPEE 273
CF CG+ GH + CP E
Sbjct: 419 CFKCGKPGHFARECPSE 469
>TC16357 weakly similar to UP|Q8DZP0 (Q8DZP0) Bacterial luciferase family
protein, partial (6%)
Length = 572
Score = 27.7 bits (60), Expect = 5.6
Identities = 15/39 (38%), Positives = 20/39 (50%), Gaps = 2/39 (5%)
Frame = -2
Query: 359 CFIAFDCVSALGLDLSDMNGEMVV--ETPAKGSVTTSLV 395
CF+ FDC L D D NGE+ E +G V +L+
Sbjct: 217 CFLIFDCGVLLSKDW*DQNGELQKD*EADRRGQVQKNLL 101
>TC9401 similar to UP|Q84LW7 (Q84LW7) Peptidylprolyl cis-trans isomerase ,
partial (97%)
Length = 918
Score = 27.7 bits (60), Expect = 5.6
Identities = 15/44 (34%), Positives = 23/44 (52%), Gaps = 5/44 (11%)
Frame = +1
Query: 505 PDVPPEREVEFSIDLV-----PGTKPVSMAPYRISASELKKQLE 543
PD+PP+ + F ++L+ G+ S+ R ELKKQ E
Sbjct: 469 PDIPPDATLVFEVELLACNPRKGSSLGSVTEERARLEELKKQRE 600
>AV773507
Length = 496
Score = 27.7 bits (60), Expect = 5.6
Identities = 8/15 (53%), Positives = 10/15 (66%)
Frame = +2
Query: 257 CFNCGEKGHKSNACP 271
CF CG GH++ CP
Sbjct: 350 CFECGRPGHRARDCP 394
>TC8573 similar to UP|Q9SIH5 (Q9SIH5) At2g36090 protein, partial (17%)
Length = 515
Score = 27.3 bits (59), Expect = 7.4
Identities = 17/54 (31%), Positives = 29/54 (53%), Gaps = 1/54 (1%)
Frame = +2
Query: 474 SLMATLSIENQAVI-DRLPVVCDFPEVFPDEIPDVPPEREVEFSIDLVPGTKPV 526
SL++T ++++ D P + D P P ++P PP E+ ++DL KPV
Sbjct: 323 SLISTFPTAHRSIFSDSFPSLHDSP--LPHKLPPSPPPPELISAVDLYYKGKPV 478
>TC16235
Length = 411
Score = 26.9 bits (58), Expect = 9.6
Identities = 10/16 (62%), Positives = 11/16 (68%)
Frame = +1
Query: 265 HKSNACPEEIKKCVRC 280
H+SN CP IK C RC
Sbjct: 169 HRSNRCPRLIKICRRC 216
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.320 0.136 0.411
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,873,664
Number of Sequences: 28460
Number of extensions: 130774
Number of successful extensions: 707
Number of sequences better than 10.0: 38
Number of HSP's better than 10.0 without gapping: 646
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 684
length of query: 615
length of database: 4,897,600
effective HSP length: 96
effective length of query: 519
effective length of database: 2,165,440
effective search space: 1123863360
effective search space used: 1123863360
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 58 (26.9 bits)
Medicago: description of AC146333.2