BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146333.19 - phase: 0 /pseudo
(786 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP043969 140 8e-34
TC14579 similar to UP|O23866 (O23866) MEI2-like protein, partial... 84 1e-16
TC12437 62 5e-10
TC14336 similar to UP|Q9M549 (Q9M549) Poly(A)-binding protein, p... 40 0.001
TC18938 similar to UP|AAS00039 (AAS00039) Splicing factor-like p... 40 0.002
TC14208 similar to UP|Q9LEB4 (Q9LEB4) RNA binding protein 45, pa... 38 0.005
TC14791 similar to UP|Q9LJ04 (Q9LJ04) ESTs AU082210(C53655), par... 37 0.012
TC14392 similar to UP|Q9LEB4 (Q9LEB4) RNA binding protein 45, pa... 37 0.012
TC10026 35 0.035
TC11829 weakly similar to UP|NAM8_YEAST (Q00539) NAM8 protein, p... 35 0.035
TC8052 similar to GB|AAP13431.1|30023796|BT006323 At5g02530 {Ara... 35 0.035
TC13693 similar to PIR|T00933|T00933 RNA-binding protein homolog... 35 0.061
TC19849 similar to UP|Q9AT32 (Q9AT32) Poly(A)-binding protein, p... 33 0.13
AV425247 33 0.18
BP043331 32 0.30
TC12481 32 0.30
TC16163 similar to UP|Q9LRR6 (Q9LRR6) Similarity to RNA-binding ... 32 0.39
TC19711 31 0.67
TC17697 31 0.87
BP036473 30 1.9
>BP043969
Length = 445
Score = 140 bits (353), Expect = 8e-34
Identities = 67/80 (83%), Positives = 71/80 (88%)
Frame = -1
Query: 707 NVGYAFINMTSPSLIVPFYQGFNGKKWEKFNSEKVASLAYARIQGKAALVAHFQNSSLMN 766
NVGYAFINM SPS IVPF++ FNGKKWEKFNSEKVASLAYARIQGKAALV HFQNSSLMN
Sbjct: 445 NVGYAFINMVSPSHIVPFFKAFNGKKWEKFNSEKVASLAYARIQGKAALVMHFQNSSLMN 266
Query: 767 EDKRCRPILIDTDGPNAGDQ 786
EDKRCRPIL ++G GDQ
Sbjct: 265 EDKRCRPILFHSEGQGTGDQ 206
>TC14579 similar to UP|O23866 (O23866) MEI2-like protein, partial (7%)
Length = 715
Score = 83.6 bits (205), Expect = 1e-16
Identities = 39/44 (88%), Positives = 41/44 (92%)
Frame = +2
Query: 742 ASLAYARIQGKAALVAHFQNSSLMNEDKRCRPILIDTDGPNAGD 785
A LAYARIQGK+AL+AHFQNSSLMNEDKRCRPIL TDGPNAGD
Sbjct: 2 AVLAYARIQGKSALIAHFQNSSLMNEDKRCRPILFHTDGPNAGD 133
>TC12437
Length = 749
Score = 61.6 bits (148), Expect = 5e-10
Identities = 37/64 (57%), Positives = 42/64 (64%)
Frame = -1
Query: 40 ASRHESSLFSSSLSDLFSKKLRLSANNAFYGHSVDTIASNYEEEKLSDSLEELEAQIIGN 99
A ESSLFSSSLSD+FS+KLRL N + T+ S EEE SLEE+EA IGN
Sbjct: 188 AGSRESSLFSSSLSDIFSQKLRLMGNGVLSDQHI-TVGSLPEEEPFK-SLEEIEADTIGN 15
Query: 100 LLPD 103
LLPD
Sbjct: 14 LLPD 3
>TC14336 similar to UP|Q9M549 (Q9M549) Poly(A)-binding protein, partial
(85%)
Length = 1917
Score = 40.0 bits (92), Expect = 0.001
Identities = 35/147 (23%), Positives = 62/147 (41%), Gaps = 7/147 (4%)
Frame = +2
Query: 179 LFVRNIDSDVKDSVLKALFEQFGDIHT----FDRTCKHQGSAMISYYDIRAAQNAMRALN 234
+F++N+D + L F FG+I + D + + +G + + AAQNA+ LN
Sbjct: 98 IFIKNLDKAIDHKALHDTFSTFGNILSCKVATDSSGQSKGYGFVQFETEEAAQNAIEKLN 277
Query: 235 NRLFGRKKFDIHYPIPKDSPSRNGVNQGTLEVFLYDSSISNT--ELQHILNVYGGI-KEI 291
L K+ + + K + VF+ + S S T EL+++ +G I +
Sbjct: 278 GMLLNDKQVYVGPFLRKQERESSTDKAKFNNVFVKNLSESTTDDELKNVFGEFGTITSAV 457
Query: 292 HENPRSQRHKLIEFYDFRAADAALHGI 318
+ K F +F AD A +
Sbjct: 458 VMRDGDGKSKCFGFVNFENADDAARAV 538
>TC18938 similar to UP|AAS00039 (AAS00039) Splicing factor-like protein,
partial (18%)
Length = 473
Score = 39.7 bits (91), Expect = 0.002
Identities = 24/65 (36%), Positives = 34/65 (51%)
Frame = +3
Query: 177 RTLFVRNIDSDVKDSVLKALFEQFGDIHTFDRTCKHQGSAMISYYDIRAAQNAMRALNNR 236
R++F N + D + S + LF ++G + DR G A I D R A A+RAL+
Sbjct: 219 RSIFCGNFEYDARQSESERLFRRYGKV---DRVDMKSGFAFIYMEDERDADAAIRALDRA 389
Query: 237 LFGRK 241
FGRK
Sbjct: 390 EFGRK 404
>TC14208 similar to UP|Q9LEB4 (Q9LEB4) RNA binding protein 45, partial (41%)
Length = 837
Score = 38.1 bits (87), Expect = 0.005
Identities = 22/74 (29%), Positives = 37/74 (49%), Gaps = 1/74 (1%)
Frame = +2
Query: 167 GENSYGEHPSRTLFVRNIDSDVKDSVLKALFEQFGD-IHTFDRTCKHQGSAMISYYDIRA 225
G + + + T+FV N+D +V D L+ +F +G+ +H K G + + D
Sbjct: 161 GSQNENDPSNTTIFVGNLDPNVNDDHLRQVFSPYGELVHVKIPAGKRCG--FVQFADRSG 334
Query: 226 AQNAMRALNNRLFG 239
A+ A+R LN L G
Sbjct: 335 AEEALRVLNGTLLG 376
>TC14791 similar to UP|Q9LJ04 (Q9LJ04) ESTs AU082210(C53655), partial (37%)
Length = 1779
Score = 37.0 bits (84), Expect = 0.012
Identities = 21/72 (29%), Positives = 37/72 (51%), Gaps = 5/72 (6%)
Frame = +2
Query: 173 EHPSRTLFVRNIDSDVKDSVLKALFEQFGDIHT----FDR-TCKHQGSAMISYYDIRAAQ 227
E+ R +FV N+ SD++ L F+QFG++ D+ T + +G A+ Y + +A+
Sbjct: 884 EYTQRKIFVSNVSSDIEPLKLLEFFKQFGEVEDGPLGLDKNTGRPKGFALFVYRSVESAK 1063
Query: 228 NAMRALNNRLFG 239
A+ N G
Sbjct: 1064KALEEPNKEYEG 1099
>TC14392 similar to UP|Q9LEB4 (Q9LEB4) RNA binding protein 45, partial (77%)
Length = 1576
Score = 37.0 bits (84), Expect = 0.012
Identities = 23/74 (31%), Positives = 37/74 (49%), Gaps = 1/74 (1%)
Frame = +3
Query: 167 GENSYGEHPSRTLFVRNIDSDVKDSVLKALFEQFGD-IHTFDRTCKHQGSAMISYYDIRA 225
G + + + T+FV N+D +V D L+ +F +GD +H K G + + D
Sbjct: 912 GPQNENDPNNTTIFVGNLDPNVTDDHLRQVFGLYGDLVHVKIPQGKRCG--FVQFADRSC 1085
Query: 226 AQNAMRALNNRLFG 239
A+ A+R LN L G
Sbjct: 1086AEEALRVLNGTLLG 1127
>TC10026
Length = 599
Score = 35.4 bits (80), Expect = 0.035
Identities = 38/164 (23%), Positives = 77/164 (46%), Gaps = 1/164 (0%)
Frame = +2
Query: 37 DIVASRHESSLFSSSLSDLFSKKLRLSANNAFYGHSVDT-IASNYEEEKLSDSLEELEAQ 95
DI SR +SLF+ LSD L+ SA + D +++ Y+ +K ++ L + A
Sbjct: 119 DIAHSRVSASLFALPLSD----ALKASALTRLHLPDADAFLSAEYDHDKAAEVLRDYIAA 286
Query: 96 IIGNLLPDEDDLLSGVTDGNNYIICDSNGDDIDELDLFSSNGGFDLGDVENPSSIERNSE 155
I L ++ +++ + DG+ + +D D+ + + N DL DV++ I ++
Sbjct: 287 IADEL--KDNPIVASILDGSTISLL---LEDEDDFAMLAENLFTDL-DVKDKGKISKSEI 448
Query: 156 IISGVRNSSIAGENSYGEHPSRTLFVRNIDSDVKDSVLKALFEQ 199
+ V+ + G + E P ++ +D ++ + +A F Q
Sbjct: 449 RNALVQMGAEMGVPPFSEFPLLNDILKKHGADGEEELGQAQFAQ 580
>TC11829 weakly similar to UP|NAM8_YEAST (Q00539) NAM8 protein, partial (4%)
Length = 738
Score = 35.4 bits (80), Expect = 0.035
Identities = 30/101 (29%), Positives = 43/101 (41%), Gaps = 6/101 (5%)
Frame = +1
Query: 146 NPSSIERNSEIISGVRNSSIAGENSYGEHPSRTLFVRNIDSDVKDSVLKALFEQFGDIHT 205
NP N G + + E L++ + ++D L LF+QFG+I
Sbjct: 124 NPPWANNNGGSTGGAAQAGLGAGAVKKEIDDTNLYIGYLPPTLEDDGLIQLFQQFGEIVM 303
Query: 206 F----DR-TCKHQGSAMISYYDIRAAQNAMRALNN-RLFGR 240
DR T +G + Y DI A NA+ A+N RL GR
Sbjct: 304 AKVIKDRMTGLSKGYGFVKYADITMANNAILAMNGYRLEGR 426
>TC8052 similar to GB|AAP13431.1|30023796|BT006323 At5g02530 {Arabidopsis
thaliana;}, partial (55%)
Length = 1389
Score = 35.4 bits (80), Expect = 0.035
Identities = 25/89 (28%), Positives = 44/89 (49%), Gaps = 9/89 (10%)
Frame = +2
Query: 179 LFVRNIDSDVKDSVLKALFEQFGDI--HT--FDRTCKHQGSAMISYYDIRAAQNAMRALN 234
L++ N+D V +K LF + GD+ HT +DR+ + +G+A + + A+ A++ N
Sbjct: 407 LYISNLDYGVSSDDIKELFSEVGDLKRHTVHYDRSGRSKGTAEVVFSRRGDAEAAVKRYN 586
Query: 235 NRLFGRKKFDI-----HYPIPKDSPSRNG 258
N K I + P +P+ NG
Sbjct: 587 NVQLDGKPMKIEIVGTNITTPAVAPAANG 673
>TC13693 similar to PIR|T00933|T00933 RNA-binding protein homolog At2g42240
- Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(53%)
Length = 662
Score = 34.7 bits (78), Expect = 0.061
Identities = 28/87 (32%), Positives = 38/87 (43%), Gaps = 3/87 (3%)
Frame = +3
Query: 174 HPSRTLFVRNIDSDVKDSVLKALFEQF---GDIHTFDRTCKHQGSAMISYYDIRAAQNAM 230
H RTLFV + DVK + LF +F H T Q A + + ++A AM
Sbjct: 189 HEVRTLFVAGLPEDVKPREIYNLFREFPGYESSHLRSPTNSSQPFAFAVFMNQQSAVMAM 368
Query: 231 RALNNRLFGRKKFDIHYPIPKDSPSRN 257
ALN +F +K Y S SR+
Sbjct: 369 HALNGMVFDLEKNSTLYIDLAKSNSRS 449
>TC19849 similar to UP|Q9AT32 (Q9AT32) Poly(A)-binding protein, partial
(30%)
Length = 603
Score = 33.5 bits (75), Expect = 0.13
Identities = 19/82 (23%), Positives = 39/82 (47%), Gaps = 8/82 (9%)
Frame = +2
Query: 168 ENSYGEHPSRTLFVRNIDSDVKDSVLKALFEQFGDIHTFDRTCK--------HQGSAMIS 219
+ + ++ L+++N+D +V D L+ LF +FG I +CK +GS ++
Sbjct: 209 QETVDKYQGANLYLKNLDDNVDDEKLRELFSEFGTI----TSCKIMRDPHGVSRGSGFVA 376
Query: 220 YYDIRAAQNAMRALNNRLFGRK 241
+ A A+ +N ++ K
Sbjct: 377 FSTPEEATRALGEMNGKMVDGK 442
>AV425247
Length = 417
Score = 33.1 bits (74), Expect = 0.18
Identities = 26/72 (36%), Positives = 35/72 (48%), Gaps = 2/72 (2%)
Frame = +2
Query: 507 ISSSFPLPVHHEQWSNSYPPPRTIWPNSPSYFDGIYAASTLQRLNQLPMSPS--HMITTV 564
IS FP P+ N+ PPP T+ P+SPS I+ + SPS H+I
Sbjct: 221 ISFPFPFPITPSPSPNT-PPPLTLCPSSPSPLALIFPPTA---------SPSHPHLICPK 370
Query: 565 LPTNNHHIQSPP 576
PT+ H+ SPP
Sbjct: 371 QPTS--HLLSPP 400
>BP043331
Length = 420
Score = 32.3 bits (72), Expect = 0.30
Identities = 27/105 (25%), Positives = 47/105 (44%), Gaps = 10/105 (9%)
Frame = +3
Query: 153 NSEIISG-VRNSSIAGE--NSYGEHPSRTLFVRNIDSDVKDSVLKALFEQFGDIHTFDRT 209
N +IIS +R+ +A N P+ LFV I D L F GD++ T
Sbjct: 42 NRQIISSEIRSRFLATRSINKAASAPTSMLFVGGISPTTTDRHLDDAFSIHGDVYL--AT 215
Query: 210 CKH-------QGSAMISYYDIRAAQNAMRALNNRLFGRKKFDIHY 247
KH + ++++ + A A++ALN ++ ++ I+Y
Sbjct: 216 VKHTKVDGKPRQFGLVAFTTVECATRALQALNGQVLQGRRLRINY 350
>TC12481
Length = 693
Score = 32.3 bits (72), Expect = 0.30
Identities = 27/85 (31%), Positives = 42/85 (48%), Gaps = 4/85 (4%)
Frame = +3
Query: 20 HVDSDQGIKPFEDSKEDDIVASRHESSLFSSSLSDLFSKKLRLSANNAFYGHSVDTIASN 79
+V G P+ D + ++SLFSSSL L +KL L+ Y SVD I+S
Sbjct: 441 NVPGKPGRGPWGVLSGSDSYHASSDASLFSSSLPVLPHEKLNLNDTENNY-QSVDDISSG 617
Query: 80 Y----EEEKLSDSLEELEAQIIGNL 100
+ ++ ++DSLE + IG +
Sbjct: 618 FKKLHQDVAVNDSLENGDNDAIGTM 692
>TC16163 similar to UP|Q9LRR6 (Q9LRR6) Similarity to RNA-binding protein,
partial (38%)
Length = 762
Score = 32.0 bits (71), Expect = 0.39
Identities = 25/91 (27%), Positives = 42/91 (45%), Gaps = 3/91 (3%)
Frame = +1
Query: 176 SRTLFVRNIDSDVKDSVLKALFEQ-FGDIHTFDRTCKHQGSAMISYYDIRAAQNAMRALN 234
SRT++ NID + + +K FE G++ H S I++ + A++A+ ALN
Sbjct: 106 SRTVYCTNIDKKISQAEVKNFFESACGEVTRLRLLGDHVHSTRIAFVEFALAESAIVALN 285
Query: 235 --NRLFGRKKFDIHYPIPKDSPSRNGVNQGT 263
L G + + P +P R V + T
Sbjct: 286 CSGMLLGTQPVRVS---PSKTPVRPRVTRPT 369
>TC19711
Length = 579
Score = 31.2 bits (69), Expect = 0.67
Identities = 23/70 (32%), Positives = 39/70 (54%), Gaps = 5/70 (7%)
Frame = -2
Query: 298 QRHK--LIEFYDFRAADAALHGINRNDTTMKRLKVDQMQSTNSESNIIQPMHPEFK---Q 352
Q+H+ LI FY A +LH ++ T KRL++DQ + ++NII + +FK +
Sbjct: 539 QQHRSLLILFY----AKKSLHRLHSGKT--KRLEIDQKKRQEKQNNIINILR*KFKIYIK 378
Query: 353 ECDLCLHQKS 362
+ CLH ++
Sbjct: 377 KKHRCLHDRA 348
>TC17697
Length = 565
Score = 30.8 bits (68), Expect = 0.87
Identities = 16/63 (25%), Positives = 30/63 (47%), Gaps = 7/63 (11%)
Frame = -1
Query: 571 HIQSPPFWDRRYTYAAEPITPHCVDFVPHNMFP-------HFGLNVHNQRGMVFPGRNHM 623
H+Q W++++ + PH +D +PH++ H +H +R FP +H
Sbjct: 250 HLQLAQTWEKKFLQSQ----PHMIDQIPHHLLHSL*HCLFHQWQKLHRERN--FPSHDHQ 89
Query: 624 INS 626
I+S
Sbjct: 88 IDS 80
>BP036473
Length = 510
Score = 29.6 bits (65), Expect = 1.9
Identities = 16/57 (28%), Positives = 31/57 (54%), Gaps = 2/57 (3%)
Frame = +3
Query: 176 SRTLFVRNIDSDVKDSVLKALFEQFGDIHTFDRTC--KHQGSAMISYYDIRAAQNAM 230
SRT++V N+ D++ ++ +F +FG D + G A + + D R A++A+
Sbjct: 69 SRTIYVGNLPGDIRLREVEDIFYKFGPXVDIDLKIPPRPPGYAFVQFEDARDAEDAI 239
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.324 0.139 0.419
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,052,944
Number of Sequences: 28460
Number of extensions: 213783
Number of successful extensions: 1516
Number of sequences better than 10.0: 66
Number of HSP's better than 10.0 without gapping: 1492
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1509
length of query: 786
length of database: 4,897,600
effective HSP length: 97
effective length of query: 689
effective length of database: 2,136,980
effective search space: 1472379220
effective search space used: 1472379220
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 59 (27.3 bits)
Medicago: description of AC146333.19