BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146332.5 + phase: 0
(220 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV428062 124 2e-29
TC9931 weakly similar to GB|AAO39953.1|28372942|BT003725 At5g152... 111 1e-25
TC10395 weakly similar to UP|Q93XN3 (Q93XN3) Salicylic acid-indu... 46 6e-06
BF177670 42 6e-05
TC17823 similar to UP|Q93XN3 (Q93XN3) Salicylic acid-induced fra... 39 0.001
AV416034 34 0.017
AV781219 34 0.023
TC17503 31 0.15
TC11492 28 1.2
TC13757 similar to UP|Q9LT35 (Q9LT35) Kinase-like protein, parti... 27 2.1
TC11173 26 4.7
BP034977 26 6.2
BP034485 26 6.2
TC18830 weakly similar to UP|O40637 (O40637) Protease, partial (5%) 26 6.2
TC10367 26 6.2
TC14538 similar to GB|AAH54382.1|32450414|BC054382 feminization ... 26 6.2
TC18832 homologue to UP|O04091 (O04091) Endomembrane protein EMP... 26 6.2
TC12762 26 6.2
TC18197 25 8.0
BP033927 25 8.0
>AV428062
Length = 386
Score = 124 bits (310), Expect = 2e-29
Identities = 60/101 (59%), Positives = 72/101 (70%)
Frame = +3
Query: 40 ATVVTTKATPLQKGGMKKGIAILDFILRLGAIGAALGAAVIMGTNEQILPFFTQFLQFHA 99
A + ATP++ GG KKGIAI+DF+LRLGAI AALGAA M T +Q LPFFTQF QF A
Sbjct: 84 AAKAVSVATPIRPGGWKKGIAIVDFVLRLGAIAAALGAATTMATADQTLPFFTQFFQFEA 263
Query: 100 QWDDFPMFKFFVVANGAAAGFLILSLPFSIVCIVRPLAAGP 140
+D F F+FFV+ G+L+LSLP SIV I+RP A GP
Sbjct: 264 SYDSFTTFQFFVITMALVGGYLVLSLPLSIVTIIRPYAVGP 386
>TC9931 weakly similar to GB|AAO39953.1|28372942|BT003725 At5g15290
{Arabidopsis thaliana;}, partial (60%)
Length = 609
Score = 111 bits (277), Expect = 1e-25
Identities = 53/119 (44%), Positives = 81/119 (67%)
Frame = +2
Query: 101 WDDFPMFKFFVVANGAAAGFLILSLPFSIVCIVRPLAAGPRFLLVIVDLVLMALVVAAAS 160
+DD P + FVVA+ A+G+LILSLP SI+ I R A R +L+I+D+V++ + AA+S
Sbjct: 2 YDDLPTLRLFVVASAFASGYLILSLPLSILHITRSRARRTRVILIILDMVMLTSLTAASS 181
Query: 161 SAAAVVYLAHNGSQDANWNAICQQFTDFCQGSSLAVVASFVASVFLACLVVVSSVALKR 219
+AAA+VYLAH G+ ANW A CQQ+ FC+ S +++ SF+A L++ S++ L +
Sbjct: 182 AAAAIVYLAHKGNAKANWFAFCQQYDSFCERISGSLIGSFIAIPLFIMLILFSALVLSK 358
>TC10395 weakly similar to UP|Q93XN3 (Q93XN3) Salicylic acid-induced
fragment 1 protein (Fragment), partial (44%)
Length = 617
Score = 45.8 bits (107), Expect = 6e-06
Identities = 21/77 (27%), Positives = 41/77 (52%)
Frame = -2
Query: 141 RFLLVIVDLVLMALVVAAASSAAAVVYLAHNGSQDANWNAICQQFTDFCQGSSLAVVASF 200
++ + V +L +++AA + + V+YL G A W++ C F FC ++ ++ +F
Sbjct: 613 KYASIYVPSLLTYIILAAGALSMEVLYLTEKGDPPAAWSSACGYFGPFCHKATASIAITF 434
Query: 201 VASVFLACLVVVSSVAL 217
VA + L ++SS L
Sbjct: 433 VAVICYVVLSLISSYKL 383
>BF177670
Length = 341
Score = 42.4 bits (98), Expect = 6e-05
Identities = 27/98 (27%), Positives = 47/98 (47%)
Frame = +1
Query: 122 ILSLPFSIVCIVRPLAAGPRFLLVIVDLVLMALVVAAASSAAAVVYLAHNGSQDANWNAI 181
+LS+ V +PLA + + D V+ + VAA ++AA A G + W I
Sbjct: 19 VLSMIRGKVLFSKPLA----WAIFSGDQVMAYITVAAVAAAAQSAMFAKLGQPELQWMKI 186
Query: 182 CQQFTDFCQGSSLAVVASFVASVFLACLVVVSSVALKR 219
C + FC + ++F+AS+ + L +S+ +L R
Sbjct: 187 CDMYGKFCNQVGEGLASAFLASLSMVVLSCISAFSLFR 300
>TC17823 similar to UP|Q93XN3 (Q93XN3) Salicylic acid-induced fragment 1
protein (Fragment), partial (32%)
Length = 363
Score = 38.5 bits (88), Expect = 0.001
Identities = 28/99 (28%), Positives = 47/99 (47%)
Frame = +3
Query: 59 IAILDFILRLGAIGAALGAAVIMGTNEQILPFFTQFLQFHAQWDDFPMFKFFVVANGAAA 118
+ I++ LRL IG + A VIM N Q + T + D F++ V ANG A
Sbjct: 90 LRIVETFLRLFPIGLCVTALVIMLKNSQENEYGT------LDYTDLSAFRYLVHANGICA 251
Query: 119 GFLILSLPFSIVCIVRPLAAGPRFLLVIVDLVLMALVVA 157
G+ + S I+ + RP + ++D +L +++A
Sbjct: 252 GYSLFSA--VIIALPRPSTMPRAWTFFVLDQLLTYIILA 362
>AV416034
Length = 392
Score = 34.3 bits (77), Expect = 0.017
Identities = 25/74 (33%), Positives = 36/74 (47%)
Frame = +2
Query: 62 LDFILRLGAIGAALGAAVIMGTNEQILPFFTQFLQFHAQWDDFPMFKFFVVANGAAAGFL 121
L+ LRL IG + A V+M N Q + + + D F++ V ANG AG+
Sbjct: 134 LETFLRLLPIGLCVSALVLMLKNSQENDYGS------VDYTDLGPFRYLVHANGICAGYS 295
Query: 122 ILSLPFSIVCIVRP 135
+LS F V + RP
Sbjct: 296 LLSAVF--VAVPRP 331
>AV781219
Length = 435
Score = 33.9 bits (76), Expect = 0.023
Identities = 19/55 (34%), Positives = 30/55 (54%)
Frame = -2
Query: 123 LSLPFSIVCIVRPLAAGPRFLLVIVDLVLMALVVAAASSAAAVVYLAHNGSQDAN 177
+SLP SI+ IVR PRF L+I+D V ++ + A + + H+ S + N
Sbjct: 329 ISLPLSILAIVRTHVVRPRFFLIILDPVSSPTLIYFSKPA*ML*DIGHSSSFNLN 165
>TC17503
Length = 564
Score = 31.2 bits (69), Expect = 0.15
Identities = 27/104 (25%), Positives = 42/104 (39%), Gaps = 4/104 (3%)
Frame = +3
Query: 107 FKFFVVANGAAAGFLILSLPFSIVCIVRPLAAGPRFLLVIVDL----VLMALVVAAASSA 162
F+F + AN A + + + S+ I R P L V D V L++AA+++
Sbjct: 9 FRFVLAANAIVAVYSLFEMVASVWEISRGATVFPEVLQVWFDFGHDQVFAYLLLAASAAG 188
Query: 163 AAVVYLAHNGSQDANWNAICQQFTDFCQGSSLAVVASFVASVFL 206
A+V + C FC S +AV + VFL
Sbjct: 189 TAMV-------RTLKETDTCTASNAFCVQSDIAVALGYAGFVFL 299
>TC11492
Length = 789
Score = 28.1 bits (61), Expect = 1.2
Identities = 27/103 (26%), Positives = 41/103 (39%), Gaps = 4/103 (3%)
Frame = +2
Query: 108 KFFVVANGAAAGFLILSLPFSIVCIVRPLAAGPRFLLVIVDL----VLMALVVAAASSAA 163
+F + AN A + + + S+ I R P L V D V L++AA+++
Sbjct: 2 RFVLAANAIVAVYSLFEMVASVWEISRGATVFPEVLQVWFDFGHDQVFAYLLLAASAAGT 181
Query: 164 AVVYLAHNGSQDANWNAICQQFTDFCQGSSLAVVASFVASVFL 206
A+V NA C Q S +AV + VFL
Sbjct: 182 AMVRTLKEMDTCTASNAFCLQ-------SDIAVALGYAGFVFL 289
>TC13757 similar to UP|Q9LT35 (Q9LT35) Kinase-like protein, partial (12%)
Length = 500
Score = 27.3 bits (59), Expect = 2.1
Identities = 14/43 (32%), Positives = 24/43 (55%)
Frame = -2
Query: 157 AAASSAAAVVYLAHNGSQDANWNAICQQFTDFCQGSSLAVVAS 199
A++SS + + AHN S+D+ +A+C Q S ++V S
Sbjct: 214 ASSSSLSCLSS*AHNSSRDSRASALCWQGLSLIVSSPSSIVVS 86
>TC11173
Length = 667
Score = 26.2 bits (56), Expect = 4.7
Identities = 14/44 (31%), Positives = 22/44 (49%)
Frame = -3
Query: 63 DFILRLGAIGAALGAAVIMGTNEQILPFFTQFLQFHAQWDDFPM 106
D +LR A+ L A +I+ N++ +QF A + FPM
Sbjct: 236 DVLLRFLALSLTLVATIIVAVNKETKTISYAGMQFKATXNMFPM 105
>BP034977
Length = 415
Score = 25.8 bits (55), Expect = 6.2
Identities = 16/39 (41%), Positives = 22/39 (56%), Gaps = 5/39 (12%)
Frame = +2
Query: 37 PHAATVVTTKATPLQKGGMKKG-----IAILDFILRLGA 70
P+AAT+VT TP K K+G I L F+L+ G+
Sbjct: 62 PNAATMVTITITPAFKTN*KRGKKEM*IRTLKFLLKNGS 178
>BP034485
Length = 485
Score = 25.8 bits (55), Expect = 6.2
Identities = 19/71 (26%), Positives = 30/71 (41%)
Frame = -1
Query: 61 ILDFILRLGAIGAALGAAVIMGTNEQILPFFTQFLQFHAQWDDFPMFKFFVVANGAAAGF 120
I+ + L AL M + +IL F+QF + F F FVV++
Sbjct: 371 IMAIFIALPTTATALATTFTMTRDVKILEIKVNFIQFPSSSSTFLSFFPFVVSSS----- 207
Query: 121 LILSLPFSIVC 131
++L L +VC
Sbjct: 206 IVLVLVLVLVC 174
>TC18830 weakly similar to UP|O40637 (O40637) Protease, partial (5%)
Length = 850
Score = 25.8 bits (55), Expect = 6.2
Identities = 20/73 (27%), Positives = 32/73 (43%), Gaps = 7/73 (9%)
Frame = -2
Query: 101 WDDFPMFKFFVVANGAAAGFLILSLPFSIVCIVRPLAAGPRFL-------LVIVDLVLMA 153
W FP FKF + + A S+ SI C+ RP + L +V+ D L
Sbjct: 735 WKSFPAFKFCITFDMAR-----YSISASIFCLKRPSTSDSTSLHISSDKPMVLADSSLGD 571
Query: 154 LVVAAASSAAAVV 166
L + A S++ ++
Sbjct: 570 LPLLKAVSSSTIL 532
>TC10367
Length = 527
Score = 25.8 bits (55), Expect = 6.2
Identities = 14/37 (37%), Positives = 21/37 (55%)
Frame = +1
Query: 163 AAVVYLAHNGSQDANWNAICQQFTDFCQGSSLAVVAS 199
A++ +L NGS+ A T FC G++LAV +S
Sbjct: 361 ASINWLKENGSKKAG-------VTGFCMGAALAVASS 450
>TC14538 similar to GB|AAH54382.1|32450414|BC054382 feminization 1 homolog a
{Mus musculus;}, partial (3%)
Length = 2631
Score = 25.8 bits (55), Expect = 6.2
Identities = 16/55 (29%), Positives = 25/55 (45%)
Frame = -3
Query: 113 ANGAAAGFLILSLPFSIVCIVRPLAAGPRFLLVIVDLVLMALVVAAASSAAAVVY 167
AN + A LIL+ P S++C V P +L + L+ A + A V+
Sbjct: 1045 ANLSTAASLILAKPCSVLCRVEPRLENTMCILAGIALLARPFAKLRAGNTAIRVF 881
>TC18832 homologue to UP|O04091 (O04091) Endomembrane protein EMP70 precusor
isolog, 68664-64364, partial (31%)
Length = 795
Score = 25.8 bits (55), Expect = 6.2
Identities = 21/82 (25%), Positives = 36/82 (43%)
Frame = +3
Query: 87 ILPFFTQFLQFHAQWDDFPMFKFFVVANGAAAGFLILSLPFSIVCIVRPLAAGPRFLLVI 146
+LPF + F++ + + F +K + V GF++L ++ V G FLL
Sbjct: 141 LLPFGSIFIEMYFVFTSFWNYKVYYV-----YGFMLLVFLILVIVTVCVTIVGTYFLLNA 305
Query: 147 VDLVLMALVVAAASSAAAVVYL 168
+ +A+S A VYL
Sbjct: 306 ENYHWRWTSFFSAASTAVYVYL 371
>TC12762
Length = 865
Score = 25.8 bits (55), Expect = 6.2
Identities = 12/36 (33%), Positives = 22/36 (60%)
Frame = -2
Query: 124 SLPFSIVCIVRPLAAGPRFLLVIVDLVLMALVVAAA 159
SLPF C++ P + G R+ L+++D + L++ A
Sbjct: 282 SLPFDSPCVLAPSSLG-RWALILID*LSFLLILQPA 178
>TC18197
Length = 521
Score = 25.4 bits (54), Expect = 8.0
Identities = 13/39 (33%), Positives = 17/39 (43%)
Frame = -1
Query: 11 STAPILESKRTRSNGKGKSIDGDHSPPHAATVVTTKATP 49
+TAP KR R G + H PP ++ TT P
Sbjct: 320 TTAPPTNGKRCRIRSGGATTTTIHPPPRSSLSPTTAIPP 204
>BP033927
Length = 493
Score = 25.4 bits (54), Expect = 8.0
Identities = 13/34 (38%), Positives = 19/34 (55%)
Frame = +2
Query: 3 SRREVEESSTAPILESKRTRSNGKGKSIDGDHSP 36
+RR S I+++ + R NGKG SID +P
Sbjct: 305 TRRTATRGSRGGIVDTPKKR-NGKGASIDAQFAP 403
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.325 0.136 0.396
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,479,243
Number of Sequences: 28460
Number of extensions: 44815
Number of successful extensions: 339
Number of sequences better than 10.0: 41
Number of HSP's better than 10.0 without gapping: 334
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 337
length of query: 220
length of database: 4,897,600
effective HSP length: 87
effective length of query: 133
effective length of database: 2,421,580
effective search space: 322070140
effective search space used: 322070140
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 53 (25.0 bits)
Medicago: description of AC146332.5