BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146332.3 - phase: 0
(281 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC14206 similar to PIR|T49142|T49142 CCR4-associated factor 1-li... 338 5e-94
TC9763 weakly similar to UP|CNT8_HUMAN (Q9UFF9) CCR4-NOT transcr... 236 3e-63
TC17465 similar to PIR|T49142|T49142 CCR4-associated factor 1-li... 197 2e-51
TC19252 similar to UP|Q9FMS6 (Q9FMS6) CCR4-associated factor-lik... 153 4e-38
TC15183 similar to PIR|T49142|T49142 CCR4-associated factor 1-li... 136 3e-33
TC18485 similar to UP|Q9LEU4 (Q9LEU4) CCR4-associated factor-lik... 122 7e-29
TC19628 similar to PIR|T49142|T49142 CCR4-associated factor 1-li... 64 2e-22
TC14608 weakly similar to UP|CNO7_MOUSE (Q60809) CCR4-NOT transc... 95 2e-20
TC8854 weakly similar to UP|Q9LEU4 (Q9LEU4) CCR4-associated fact... 47 3e-06
TC15434 42 9e-05
BP058189 26 8.7
TC14470 similar to UP|AAQ56728 (AAQ56728) Polygalacturonase inhi... 26 8.7
>TC14206 similar to PIR|T49142|T49142 CCR4-associated factor 1-like protein
- Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(87%)
Length = 1151
Score = 338 bits (868), Expect = 5e-94
Identities = 170/282 (60%), Positives = 214/282 (75%), Gaps = 4/282 (1%)
Frame = +3
Query: 4 VKEEDASNKPIKIRQVWAENLEREFDLIRDFVHMFPYVSMDTEFPGVVVAPNFDPNIPYH 63
V+ E+ +KPI IR+VWA NLE EF LIR+ + FP++SMDTEFPGV+ P +
Sbjct: 195 VQLEEPDSKPILIREVWASNLESEFQLIREVIDEFPFISMDTEFPGVIYRPTTADTAKPY 374
Query: 64 LRHMDPSEQYSFLKANVDNLNLIQLGLTLTDANGNLP--GDVAYSYIWEFNFKDFDVDRD 121
L + PS+ Y LK+NVD LNLIQ+GLT +DA+GNLP G +IWEFNF+DFD++RD
Sbjct: 375 LNRLQPSDHYRLLKSNVDALNLIQVGLTFSDADGNLPDLGTGTNRFIWEFNFRDFDIERD 554
Query: 122 LQNPDSIELLRRQGIDFKRNLIYGVDSLEFAKLFRLKSGLV-NSGVSWVTFHSSYDFGYL 180
DSI+LLRRQGIDF+RN+ GVDS++FA+L + SGLV N VSWVTFHS+YDFGYL
Sbjct: 555 AHALDSIDLLRRQGIDFRRNVTDGVDSVDFAELV-MSSGLVCNDSVSWVTFHSAYDFGYL 731
Query: 181 VKILTQNYLPSRLEEFLSILTQIFGQNVYDMKYMIKFCN-LYGGLERVATKLKVSRAVGN 239
VK+LT+ LP+ LEEFL +L FG+NVYD+KYM++FC LYGGL+RVA L V RAVG
Sbjct: 732 VKVLTRRNLPNGLEEFLKLLRVFFGKNVYDVKYMMRFCEALYGGLDRVAQTLNVDRAVGK 911
Query: 240 SHQAASDSLLTWQAFKKMKDIYFVNNGITMHAGVLFGLEVTV 281
HQA SDSLLTW A++KM++IYF+N+ HAGVLFGLEV+V
Sbjct: 912 CHQAGSDSLLTWHAYQKMREIYFINDEPQKHAGVLFGLEVSV 1037
>TC9763 weakly similar to UP|CNT8_HUMAN (Q9UFF9) CCR4-NOT transcription
complex, subunit 8 (CAF1-like protein) (CALIFp), partial
(64%)
Length = 1152
Score = 236 bits (602), Expect = 3e-63
Identities = 128/277 (46%), Positives = 183/277 (65%), Gaps = 3/277 (1%)
Frame = +2
Query: 4 VKEEDASNKPIKIRQVWAENLEREFDLIRDFVHMFPYVSMDTEFPGVVVAP--NFDPNIP 61
++++ + I+IR+VW ENLE+EF LIR+ V +PY++MDTEFPG+V+ P NF +
Sbjct: 158 MQQDSPKAESIQIREVWNENLEQEFALIREIVDDYPYIAMDTEFPGIVLRPVGNFKNSHD 337
Query: 62 YHLRHMDPSEQYSFLKANVDNLNLIQLGLTLTDANGNLPGDVAYSYIWEFNFKDFDVDRD 121
YH Y LK NVD L LIQLGLT +D GNLP S IW+FNF++F+V+ D
Sbjct: 338 YH---------YQTLKDNVDMLKLIQLGLTFSDEEGNLPSCDGSSCIWQFNFREFNVNED 490
Query: 122 LQNPDSIELLRRQGIDFKRNLIYGVDSLEFAKLFRLKSGLVNSGVSWVTFHSSYDFGYLV 181
+ DSIELLR+ GIDF +N G+D+ F +L ++N + WVTFHS YDFGYL+
Sbjct: 491 VFANDSIELLRQSGIDFSKNNEMGIDARRFGELLMSSGIVLNDNIHWVTFHSGYDFGYLL 670
Query: 182 KILTQNYLPSRLEEFLSILTQIFGQNVYDMKYMIKFCN-LYGGLERVATKLKVSRAVGNS 240
K+LT LP F +++ ++ + D+K+++KFCN L+GGL ++A L+V R VG S
Sbjct: 671 KLLTCQDLPDTQVGFFNMI-NMYLPTLCDIKHLMKFCNSLHGGLNKLAELLEVER-VGIS 844
Query: 241 HQAASDSLLTWQAFKKMKDIYFVNNGITMHAGVLFGL 277
HQA SDSLLT F+K+K+ +F + + +AG+L+GL
Sbjct: 845 HQAGSDSLLTSCTFRKLKENFF-SGSLEKYAGILYGL 952
>TC17465 similar to PIR|T49142|T49142 CCR4-associated factor 1-like protein
- Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(57%)
Length = 538
Score = 197 bits (501), Expect = 2e-51
Identities = 101/156 (64%), Positives = 120/156 (76%), Gaps = 2/156 (1%)
Frame = +3
Query: 125 PDSIELLRRQGIDFKRNLIYGVDSLEFAKLFRLKSGLV-NSGVSWVTFHSSYDFGYLVKI 183
PDSI LL RQGIDF RN +GVDS FA+L + SGLV N VSWVTFHS+YDFGYLVKI
Sbjct: 21 PDSIALLCRQGIDFARNAAWGVDSARFAQLM-MSSGLVCNDAVSWVTFHSAYDFGYLVKI 197
Query: 184 LTQNYLPSRLEEFLSILTQIFGQNVYDMKYMIKFCN-LYGGLERVATKLKVSRAVGNSHQ 242
LT+ LP+RL+EFL I+ FG N+YD+K++I FCN LYGGL+RVA L V+RAVG HQ
Sbjct: 198 LTRRSLPTRLDEFLKIVRVFFGNNIYDIKHVIGFCNSLYGGLDRVAQSLNVNRAVGKCHQ 377
Query: 243 AASDSLLTWQAFKKMKDIYFVNNGITMHAGVLFGLE 278
A SDSLLTW AF+K++D YFV +G H VL+GLE
Sbjct: 378 AGSDSLLTWHAFQKIRDAYFVKDGHKKHVNVLYGLE 485
>TC19252 similar to UP|Q9FMS6 (Q9FMS6) CCR4-associated factor-like protein,
partial (50%)
Length = 629
Score = 153 bits (386), Expect = 4e-38
Identities = 78/143 (54%), Positives = 106/143 (73%), Gaps = 3/143 (2%)
Frame = +2
Query: 140 RNLIYGVDSLEFAKLFRLKSGLV-NSGVSWVTFHSSYDFGYLVKILTQNYLPSRLEEFLS 198
RN +G+DS FA+L + SGLV + VSWVTFHS+YDFGYLVK+LTQ LP L EFL
Sbjct: 2 RNREFGIDSFRFAELM-MSSGLVCDDAVSWVTFHSAYDFGYLVKLLTQRALPEELMEFLR 178
Query: 199 ILTQIFGQNVYDMKYMIKFC-NLYGGLERVATKLKVSRAVGNSHQAASDSLLTWQAFKKM 257
++ FG V+D+K++++FC +L+GGL+RV LKV R +G SHQA SDSLLT AF+ +
Sbjct: 179 LVRVFFGDRVFDVKHLMRFCSHLHGGLDRVCHSLKVERVIGKSHQAGSDSLLTLHAFQNI 358
Query: 258 KDIYFVN-NGITMHAGVLFGLEV 279
+++YF +G+ +AGVL+GLEV
Sbjct: 359 REVYFGQADGLVKYAGVLYGLEV 427
>TC15183 similar to PIR|T49142|T49142 CCR4-associated factor 1-like protein
- Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(31%)
Length = 446
Score = 136 bits (343), Expect = 3e-33
Identities = 69/122 (56%), Positives = 86/122 (69%), Gaps = 2/122 (1%)
Frame = +1
Query: 1 MTKVKEEDASNKPIKIRQVWAENLEREFDLIRDFVHMFPYVSMDTEFPGVVVAPNFDPNI 60
M + E+ + KP+ IR+VWA NLE EF LIR+ + +P +SMDTEFPGV+ P
Sbjct: 85 MGVMMNENQNMKPVVIREVWAFNLESEFQLIRELIDQYPIISMDTEFPGVIFR---HPAA 255
Query: 61 PYHLRHMDPSEQYSFLKANVDNLNLIQLGLTLTDANGNLP--GDVAYSYIWEFNFKDFDV 118
P+H R++ PS+ Y LKANVD LNLIQ+GLTLTDA GNLP G S+IWEFNF+DFDV
Sbjct: 256 PHHHRNLRPSDNYRLLKANVDTLNLIQVGLTLTDAAGNLPDLGSGESSFIWEFNFRDFDV 435
Query: 119 DR 120
R
Sbjct: 436 AR 441
>TC18485 similar to UP|Q9LEU4 (Q9LEU4) CCR4-associated factor-like protein,
partial (48%)
Length = 586
Score = 122 bits (306), Expect = 7e-29
Identities = 70/137 (51%), Positives = 87/137 (63%), Gaps = 3/137 (2%)
Frame = +1
Query: 14 IKIRQVWAENLEREFDLIRDFVHMFPYVSMDTEFPGVVVAP--NFDPNIPYHLRHMDPSE 71
++IR+VW +NLE EF LIR+ V F YV+MDTEFPGVV+ P NF Y+
Sbjct: 196 VQIREVWNDNLEEEFALIREIVDDFSYVAMDTEFPGVVLRPLGNFKNINDYN-------- 351
Query: 72 QYSFLKANVDNLNLIQLGLTLTDANGNLPG-DVAYSYIWEFNFKDFDVDRDLQNPDSIEL 130
Y LK NVD L LIQLGLT +D NGNLP IW+FNF++F+V D+ DSIEL
Sbjct: 352 -YQTLKDNVDMLKLIQLGLTFSDENGNLPTCGTETPCIWQFNFREFNVSEDIFASDSIEL 528
Query: 131 LRRQGIDFKRNLIYGVD 147
LR+ GID K+ G+D
Sbjct: 529 LRQSGIDLKKINEKGID 579
>TC19628 similar to PIR|T49142|T49142 CCR4-associated factor 1-like protein
- Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(30%)
Length = 537
Score = 63.5 bits (153), Expect(2) = 2e-22
Identities = 33/51 (64%), Positives = 36/51 (69%), Gaps = 1/51 (1%)
Frame = +2
Query: 76 LKANVDNLNLIQLGLTLTDANGNLPG-DVAYSYIWEFNFKDFDVDRDLQNP 125
LKANVD LNLIQ+GLTL+D G LP SYIWEFN +DFDV RD P
Sbjct: 383 LKANVDRLNLIQIGLTLSDHKGKLPTLGTPNSYIWEFNVRDFDVARDDHAP 535
Score = 57.8 bits (138), Expect(2) = 2e-22
Identities = 29/58 (50%), Positives = 37/58 (63%), Gaps = 1/58 (1%)
Frame = +1
Query: 10 SNKPIKIRQVWAENLEREFDLIRDFVHMFPYVSMDTEFPGVVVAPN-FDPNIPYHLRH 66
S I R VW+ NLE EF IR + +P +SMDTEFPGV+V P+ DP+ +H RH
Sbjct: 187 SKGSILTRSVWSSNLESEFQWIRSLIDAYPLISMDTEFPGVIVRPDTADPS--FHHRH 354
>TC14608 weakly similar to UP|CNO7_MOUSE (Q60809) CCR4-NOT transcription
complex, subunit 7 (CCR4-associated factor 1) (CAF1),
partial (34%)
Length = 600
Score = 94.7 bits (234), Expect = 2e-20
Identities = 53/109 (48%), Positives = 74/109 (67%), Gaps = 1/109 (0%)
Frame = +1
Query: 172 HSSYDFGYLVKILTQNYLPSRLEEFLSILTQIFGQNVYDMKYMIKFC-NLYGGLERVATK 230
HS YDFGYL+K+LT LP F ++ +I+ +YD+K+++KFC NL+GGL ++A
Sbjct: 1 HSGYDFGYLLKLLTCRVLPESQAGFFELI-KIYFPMLYDIKHLMKFCNNLHGGLNKLAEL 177
Query: 231 LKVSRAVGNSHQAASDSLLTWQAFKKMKDIYFVNNGITMHAGVLFGLEV 279
L+V R VG HQA SDSLLT F+K++D YF + +AGVL+GL V
Sbjct: 178 LEVDR-VGMCHQAGSDSLLTSCTFRKLRDTYF-SGSTEKYAGVLYGLGV 318
>TC8854 weakly similar to UP|Q9LEU4 (Q9LEU4) CCR4-associated factor-like
protein, partial (8%)
Length = 916
Score = 47.4 bits (111), Expect = 3e-06
Identities = 24/45 (53%), Positives = 31/45 (68%), Gaps = 2/45 (4%)
Frame = +2
Query: 237 VGNSHQAASDSLLTWQAFKKMKDIYFVN--NGITMHAGVLFGLEV 279
+G SHQ SDSLL AFKK+ ++YF N +G+ + GVL GLEV
Sbjct: 641 LGRSHQVGSDSLLILHAFKKIMEVYFGNQDDGLVKYDGVLHGLEV 775
>TC15434
Length = 1097
Score = 42.4 bits (98), Expect = 9e-05
Identities = 24/67 (35%), Positives = 35/67 (51%), Gaps = 1/67 (1%)
Frame = +1
Query: 209 YDMKYMIKFCNLYGGLERVATKLKVSRAVGNSHQAASDSLLTWQAFKKMKDIYFVNN-GI 267
Y Y++K N+ G ++ L R VG SHQ SDSLL AF+ + +YF N G+
Sbjct: 775 YGFHYLLKVENVNGKQSKI---LDFKRVVGKSHQVGSDSLLILHAFENIMKVYFGNQVGL 945
Query: 268 TMHAGVL 274
+ +L
Sbjct: 946 VNRSSIL 966
>BP058189
Length = 537
Score = 25.8 bits (55), Expect = 8.7
Identities = 10/33 (30%), Positives = 19/33 (57%)
Frame = -1
Query: 209 YDMKYMIKFCNLYGGLERVATKLKVSRAVGNSH 241
+++ ++ C L G+ R+ L VSR +G+ H
Sbjct: 417 FEISFVGWLCGLCHGVWRIPGSLAVSRGIGDGH 319
>TC14470 similar to UP|AAQ56728 (AAQ56728) Polygalacturonase inhibiting
protein, partial (93%)
Length = 1195
Score = 25.8 bits (55), Expect = 8.7
Identities = 22/75 (29%), Positives = 31/75 (41%), Gaps = 7/75 (9%)
Frame = +3
Query: 84 NLIQLGLTLTDANGNLPGDVAYSYIWEFNFKDFDVDRDLQN-------PDSIELLRRQGI 136
NLI L L G +P +FK +D L + P S+ L+ I
Sbjct: 501 NLIDLRLDRNRLTGPIPDSFG-------SFKKPGIDLTLSHNQLSGPIPTSLGLIDPNRI 659
Query: 137 DFKRNLIYGVDSLEF 151
DF RN++ G S+ F
Sbjct: 660 DFSRNMLEGDASMLF 704
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.322 0.140 0.417
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,551,238
Number of Sequences: 28460
Number of extensions: 57101
Number of successful extensions: 281
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 267
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 267
length of query: 281
length of database: 4,897,600
effective HSP length: 89
effective length of query: 192
effective length of database: 2,364,660
effective search space: 454014720
effective search space used: 454014720
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 55 (25.8 bits)
Medicago: description of AC146332.3