BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146331.9 + phase: 0
(75 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC18647 similar to UP|Q9ASR9 (Q9ASR9) At2g01320/F10A8.20, partia... 58 3e-10
BP029778 42 3e-05
AW720269 39 2e-04
TC16623 weakly similar to UP|TAP2_HUMAN (Q03519) Antigen peptide... 37 9e-04
AV407220 34 0.004
TC10978 similar to UP|Q93YY0 (Q93YY0) 68 kDa protein HP68, parti... 34 0.004
TC10604 similar to AAQ89671 (AAQ89671) At4g33460, partial (36%) 34 0.006
TC11559 similar to UP|Q9SXT3 (Q9SXT3) Multidrug resistance prote... 33 0.010
BP042433 32 0.017
TC9999 similar to UP|Q9FMU0 (Q9FMU0) Similarity to ABC transport... 32 0.017
AV413975 32 0.022
AV776921 30 0.064
AV417384 29 0.19
AV767093 29 0.19
TC7894 weakly similar to UP|Q93YY0 (Q93YY0) 68 kDa protein HP68,... 28 0.24
BP044824 28 0.32
TC18707 homologue to PIR|T02901|T02901 MSP1 protein homolog T13J... 28 0.32
TC13255 homologue to UP|Q7XXR9 (Q7XXR9) Katanin, partial (41%) 28 0.42
BI418728 27 0.54
BI418098 27 0.93
>TC18647 similar to UP|Q9ASR9 (Q9ASR9) At2g01320/F10A8.20, partial (17%)
Length = 430
Score = 58.2 bits (139), Expect = 3e-10
Identities = 27/67 (40%), Positives = 43/67 (63%)
Frame = +1
Query: 7 MVLAWEDVRVMLPNFGKGPTKRLLNGLNGFAEPGRIMAIMGPSGSGKSTLLDTLAGSLKV 66
+ + W ++ + + LL ++G A+PGR++AIMGPSGSGK+TLL+ LAG L
Sbjct: 40 VTIRWSNINCSFSDKSSKSVRFLLKNVSGEAKPGRLLAIMGPSGSGKTTLLNVLAGQLAA 219
Query: 67 TSFLNMS 73
+ L++S
Sbjct: 220 SPRLHLS 240
>BP029778
Length = 438
Score = 41.6 bits (96), Expect = 3e-05
Identities = 18/35 (51%), Positives = 27/35 (76%)
Frame = -2
Query: 28 RLLNGLNGFAEPGRIMAIMGPSGSGKSTLLDTLAG 62
+LL+ ++G PG + A+MG SG+GK+TL+D LAG
Sbjct: 281 KLLSNVSGVFAPGVLTALMGSSGAGKTTLMDVLAG 177
>AW720269
Length = 524
Score = 38.5 bits (88), Expect = 2e-04
Identities = 18/41 (43%), Positives = 27/41 (64%)
Frame = +3
Query: 29 LLNGLNGFAEPGRIMAIMGPSGSGKSTLLDTLAGSLKVTSF 69
+L ++ A I+A++GPSG+GKSTLL +AG +K F
Sbjct: 54 ILKSVSFVARSSEIVAVVGPSGTGKSTLLRIIAGRVKDRDF 176
>TC16623 weakly similar to UP|TAP2_HUMAN (Q03519) Antigen peptide
transporter 2 (APT2) (Peptide transporter TAP2) (Peptide
transporter PSF2) (Peptide supply factor 2) (PSF-2)
(Peptide transporter involved in antigen processing 2),
partial (4%)
Length = 530
Score = 36.6 bits (83), Expect = 9e-04
Identities = 19/39 (48%), Positives = 22/39 (55%)
Frame = +1
Query: 24 GPTKRLLNGLNGFAEPGRIMAIMGPSGSGKSTLLDTLAG 62
G K LL GLN G + A+MG +GSGKST L G
Sbjct: 196 GEHKDLLKGLNLTINRGEVHALMGANGSGKSTFAKVLVG 312
>AV407220
Length = 235
Score = 34.3 bits (77), Expect = 0.004
Identities = 15/32 (46%), Positives = 21/32 (64%)
Frame = +3
Query: 9 LAWEDVRVMLPNFGKGPTKRLLNGLNGFAEPG 40
L W+D+ VM+ G T+ +L GL G+AEPG
Sbjct: 141 LTWKDLTVMV-TLSNGETQNVLEGLTGYAEPG 233
>TC10978 similar to UP|Q93YY0 (Q93YY0) 68 kDa protein HP68, partial (25%)
Length = 488
Score = 34.3 bits (77), Expect = 0.004
Identities = 14/27 (51%), Positives = 20/27 (73%)
Frame = +1
Query: 39 PGRIMAIMGPSGSGKSTLLDTLAGSLK 65
PG+++ ++G +G GKST L LAG LK
Sbjct: 325 PGQVLGLVGTNGIGKSTALKVLAGKLK 405
>TC10604 similar to AAQ89671 (AAQ89671) At4g33460, partial (36%)
Length = 498
Score = 33.9 bits (76), Expect = 0.006
Identities = 16/29 (55%), Positives = 20/29 (68%)
Frame = +2
Query: 40 GRIMAIMGPSGSGKSTLLDTLAGSLKVTS 68
G+ ++GP+G GKSTLL LAG L TS
Sbjct: 233 GQFWMLLGPNGCGKSTLLKILAGLLAPTS 319
>TC11559 similar to UP|Q9SXT3 (Q9SXT3) Multidrug resistance protein
(Fragment), partial (41%)
Length = 460
Score = 33.1 bits (74), Expect = 0.010
Identities = 16/32 (50%), Positives = 22/32 (68%)
Frame = +3
Query: 29 LLNGLNGFAEPGRIMAIMGPSGSGKSTLLDTL 60
+L G++ G I+ ++GPSGSGKSTLL L
Sbjct: 135 ILKGIHLDIPKGVIVGVIGPSGSGKSTLLRAL 230
>BP042433
Length = 534
Score = 32.3 bits (72), Expect = 0.017
Identities = 16/34 (47%), Positives = 21/34 (61%)
Frame = +2
Query: 29 LLNGLNGFAEPGRIMAIMGPSGSGKSTLLDTLAG 62
L++ L EPG + I GP+GSGKS+L L G
Sbjct: 311 LVDDLTLRVEPGSNLLITGPNGSGKSSLFRVLGG 412
>TC9999 similar to UP|Q9FMU0 (Q9FMU0) Similarity to ABC transporter,
partial (43%)
Length = 656
Score = 32.3 bits (72), Expect = 0.017
Identities = 20/45 (44%), Positives = 23/45 (50%)
Frame = +3
Query: 24 GPTKRLLNGLNGFAEPGRIMAIMGPSGSGKSTLLDTLAGSLKVTS 68
G +LLN + I G SGSGK+TLL LAG K TS
Sbjct: 300 GTQLKLLNSVTFSLPEKSFGLIFGQSGSGKTTLLQLLAGINKPTS 434
>AV413975
Length = 343
Score = 32.0 bits (71), Expect = 0.022
Identities = 13/36 (36%), Positives = 23/36 (63%)
Frame = +2
Query: 25 PTKRLLNGLNGFAEPGRIMAIMGPSGSGKSTLLDTL 60
P+ +L G+ PG +A++GPSG GK+T+ + +
Sbjct: 188 PSHPVLKGITMKLHPGSKVALVGPSGGGKTTIANLI 295
>AV776921
Length = 435
Score = 30.4 bits (67), Expect = 0.064
Identities = 15/36 (41%), Positives = 23/36 (63%)
Frame = +2
Query: 25 PTKRLLNGLNGFAEPGRIMAIMGPSGSGKSTLLDTL 60
P +LN L G+I+A++G SGSGKST++ +
Sbjct: 29 PDVEILNKLCLDIPSGKIVALVGGSGSGKSTVISLI 136
>AV417384
Length = 380
Score = 28.9 bits (63), Expect = 0.19
Identities = 14/36 (38%), Positives = 21/36 (57%)
Frame = +2
Query: 27 KRLLNGLNGFAEPGRIMAIMGPSGSGKSTLLDTLAG 62
+++L +N G + + G +GSGKST L LAG
Sbjct: 239 QQILRHVNVSLHDGGALVLTGANGSGKSTFLRMLAG 346
>AV767093
Length = 603
Score = 28.9 bits (63), Expect = 0.19
Identities = 13/35 (37%), Positives = 22/35 (62%)
Frame = +1
Query: 26 TKRLLNGLNGFAEPGRIMAIMGPSGSGKSTLLDTL 60
T +L G++ + G + ++G +GSGKSTL+ L
Sbjct: 424 TPLVLKGISLTVQGGEKIGVVGRTGSGKSTLIQVL 528
>TC7894 weakly similar to UP|Q93YY0 (Q93YY0) 68 kDa protein HP68, partial
(9%)
Length = 1599
Score = 28.5 bits (62), Expect = 0.24
Identities = 13/24 (54%), Positives = 17/24 (70%)
Frame = +2
Query: 42 IMAIMGPSGSGKSTLLDTLAGSLK 65
I+ I G G+GK+TL+ LAG LK
Sbjct: 461 IIVIQGLKGTGKTTLIRMLAGELK 532
>BP044824
Length = 502
Score = 28.1 bits (61), Expect = 0.32
Identities = 13/24 (54%), Positives = 15/24 (62%)
Frame = +1
Query: 33 LNGFAEPGRIMAIMGPSGSGKSTL 56
L G GRI+ I GP SGK+TL
Sbjct: 304 LGGGLPKGRIVEIFGPESSGKTTL 375
>TC18707 homologue to PIR|T02901|T02901 MSP1 protein homolog T13J8.110 -
Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(29%)
Length = 771
Score = 28.1 bits (61), Expect = 0.32
Identities = 18/64 (28%), Positives = 31/64 (48%)
Frame = +3
Query: 10 AWEDVRVMLPNFGKGPTKRLLNGLNGFAEPGRIMAIMGPSGSGKSTLLDTLAGSLKVTSF 69
A ++++ L P +R G +P R + + GP G+GK+ L +A SF
Sbjct: 213 AMDEIKESLQELVMLPLRRPDLFKGGLLKPCRGILLFGPPGTGKTMLAXAIANEAG-ASF 389
Query: 70 LNMS 73
+N+S
Sbjct: 390 INVS 401
>TC13255 homologue to UP|Q7XXR9 (Q7XXR9) Katanin, partial (41%)
Length = 532
Score = 27.7 bits (60), Expect = 0.42
Identities = 14/39 (35%), Positives = 22/39 (55%)
Frame = +1
Query: 35 GFAEPGRIMAIMGPSGSGKSTLLDTLAGSLKVTSFLNMS 73
G P + + + GP G+GK+ L +A K T+F N+S
Sbjct: 19 GLLSPWKGILLFGPPGTGKTMLAKAVATECK-TTFFNIS 132
>BI418728
Length = 102
Score = 27.3 bits (59), Expect = 0.54
Identities = 15/27 (55%), Positives = 19/27 (69%), Gaps = 1/27 (3%)
Frame = -2
Query: 40 GRIMAIMGPSGSGKSTL-LDTLAGSLK 65
GRI+ I GP SGK+TL L T+A + K
Sbjct: 101 GRIIEIYGPESSGKTTLALHTVAEAQK 21
>BI418098
Length = 478
Score = 26.6 bits (57), Expect = 0.93
Identities = 15/38 (39%), Positives = 21/38 (54%), Gaps = 7/38 (18%)
Frame = +1
Query: 45 IMGPSGSGKSTLLDTLAGSLKV-------TSFLNMSYV 75
++GP+GSGK+ L TLA + V T+F YV
Sbjct: 175 LIGPTGSGKTLLAKTLARIVNVPFAIADATTFTQAGYV 288
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.320 0.140 0.408
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 975,994
Number of Sequences: 28460
Number of extensions: 8565
Number of successful extensions: 100
Number of sequences better than 10.0: 56
Number of HSP's better than 10.0 without gapping: 100
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 100
length of query: 75
length of database: 4,897,600
effective HSP length: 51
effective length of query: 24
effective length of database: 3,446,140
effective search space: 82707360
effective search space used: 82707360
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 48 (23.1 bits)
Medicago: description of AC146331.9