BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146331.7 - phase: 0
(377 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC15288 homologue to UP|BIOB_ARATH (P54967) Biotin synthase (Bi... 274 2e-74
TC12653 similar to UP|BIOB_ARATH (P54967) Biotin synthase (Biot... 220 3e-58
BP068645 153 5e-38
BP055644 29 1.1
TC14833 similar to UP|Q9ZPK4 (Q9ZPK4) Glutamyl-tRNA reductase (... 28 1.9
BP047231 27 5.6
BP032030 26 9.5
TC15381 similar to UP|Q9ZST9 (Q9ZST9) Tic22, partial (96%) 26 9.5
BP045076 26 9.5
>TC15288 homologue to UP|BIOB_ARATH (P54967) Biotin synthase (Biotin
synthetase) , partial (34%)
Length = 657
Score = 274 bits (701), Expect = 2e-74
Identities = 139/147 (94%), Positives = 142/147 (96%)
Frame = +3
Query: 231 SGGIIGLGEAEDDRVGLLHTLSTLPTHPESVPINALIAVKGTPLQDQKPVEIWEMIRMIA 290
SGGIIGLGEAE+DRVGLLHTLSTLPTHPESVPINALIAVKGTPLQDQKPVEIWEMIRMIA
Sbjct: 3 SGGIIGLGEAEEDRVGLLHTLSTLPTHPESVPINALIAVKGTPLQDQKPVEIWEMIRMIA 182
Query: 291 TARITMPKAMVRLSAGRVRFSVPEQALCFLAGANSIFAGEKLLTTANNDFDADQLMFKVL 350
TARI MPKAMVRLSAGRVRFS+PEQALCFLAGANSIF GEKLLTTANNDFDADQLMFKVL
Sbjct: 183 TARIVMPKAMVRLSAGRVRFSMPEQALCFLAGANSIFTGEKLLTTANNDFDADQLMFKVL 362
Query: 351 GLLPKAPSLDDDETNEAENYKEAASSS 377
GLLPKAPS DDETNEAE+ KEAASSS
Sbjct: 363 GLLPKAPSFQDDETNEAEDIKEAASSS 443
>TC12653 similar to UP|BIOB_ARATH (P54967) Biotin synthase (Biotin
synthetase) , partial (29%)
Length = 535
Score = 220 bits (560), Expect = 3e-58
Identities = 108/136 (79%), Positives = 122/136 (89%), Gaps = 1/136 (0%)
Frame = +1
Query: 1 MFWLRPILRSQSR-SSIWVLQHCNSFSTSSAAAIQAEKTIQNGPRNDWTKDEVKSIYDSP 59
MF +RPI R+ + SS+WVL +S+SS AAIQAE+TI+ GPRNDWT+DEVKSIYDSP
Sbjct: 139 MFLVRPIFRTHPKPSSLWVLH----YSSSSPAAIQAERTIKQGPRNDWTRDEVKSIYDSP 306
Query: 60 ILDLLFHGAQVHRHAHNFREVQQCTLLSVKTGGCSEDCSYCPQSSRYDTGLKGQKLLNKD 119
+LDLLFHGAQVHRHAHNFREVQQCTLLSVKTGGCSEDCSYCPQSSRYDTGLK Q+LL+K+
Sbjct: 307 VLDLLFHGAQVHRHAHNFREVQQCTLLSVKTGGCSEDCSYCPQSSRYDTGLKAQRLLDKE 486
Query: 120 AVLQAAVKAKEAGSTR 135
AV+QAA KAKEAGSTR
Sbjct: 487 AVIQAANKAKEAGSTR 534
>BP068645
Length = 438
Score = 153 bits (386), Expect = 5e-38
Identities = 84/133 (63%), Positives = 99/133 (74%), Gaps = 2/133 (1%)
Frame = +2
Query: 4 LRPILRSQSRSSIWVLQHCNSFSTSSAAAIQAEKTIQNGPRNDWTKDEVKSIYDSPIL-- 61
+RPI R+ + S + H +S+SS AAIQAE+TI+ GPR D T+DEVKSIYDSP+L
Sbjct: 47 MRPIFRTHPKPSSLGVLH---YSSSSPAAIQAERTIKQGPRKDRTRDEVKSIYDSPVLVS 217
Query: 62 DLLFHGAQVHRHAHNFREVQQCTLLSVKTGGCSEDCSYCPQSSRYDTGLKGQKLLNKDAV 121
+ G + EVQQCTLLSVKTGGCSEDCSYCPQSSRYDTGLK Q+LL K+AV
Sbjct: 218 SRVPVGLKFTDMLITSGEVQQCTLLSVKTGGCSEDCSYCPQSSRYDTGLKAQRLLEKEAV 397
Query: 122 LQAAVKAKEAGST 134
+QAA KAKEAGST
Sbjct: 398 IQAANKAKEAGST 436
>BP055644
Length = 569
Score = 29.3 bits (64), Expect = 1.1
Identities = 15/33 (45%), Positives = 21/33 (63%)
Frame = -1
Query: 1 MFWLRPILRSQSRSSIWVLQHCNSFSTSSAAAI 33
M W RP+++ S +SIW+LQ +S ST A I
Sbjct: 437 MQWARPVVKE*STTSIWILQSXDS-STYEALLI 342
>TC14833 similar to UP|Q9ZPK4 (Q9ZPK4) Glutamyl-tRNA reductase (GluTR) ,
partial (72%)
Length = 1538
Score = 28.5 bits (62), Expect = 1.9
Identities = 18/78 (23%), Positives = 39/78 (49%)
Frame = +3
Query: 110 LKGQKLLNKDAVLQAAVKAKEAGSTRFCMGAAWRDTIGRKTNFNQILEYVKEIKGMGMEV 169
LK NK+ L+ A++A+ + AWRD++ ++ Y + I+ ++
Sbjct: 756 LKEVVAANKEDRLRKAMEAQTIIAEESKQFEAWRDSLETVPTIKKLRAYAERIRVAELDK 935
Query: 170 CCTLGMLDKDQAGELKKA 187
C LG + +D + ++++A
Sbjct: 936 C--LGKMGEDISKKMRRA 983
>BP047231
Length = 476
Score = 26.9 bits (58), Expect = 5.6
Identities = 12/37 (32%), Positives = 21/37 (56%)
Frame = -2
Query: 285 MIRMIATARITMPKAMVRLSAGRVRFSVPEQALCFLA 321
M + T ++P+ V+ S GRV+F + +CFL+
Sbjct: 457 MTMLYHTRLHSIPEMKVKFSNGRVKFYIESGHICFLS 347
>BP032030
Length = 454
Score = 26.2 bits (56), Expect = 9.5
Identities = 10/43 (23%), Positives = 19/43 (43%)
Frame = +3
Query: 67 GAQVHRHAHNFREVQQCTLLSVKTGGCSEDCSYCPQSSRYDTG 109
G +H++ HN + Q ++ + G + C Y R + G
Sbjct: 291 GCHLHQYFHNHQRH*QSSMAQKHSNGSQQSCPYAQHVERINQG 419
>TC15381 similar to UP|Q9ZST9 (Q9ZST9) Tic22, partial (96%)
Length = 1170
Score = 26.2 bits (56), Expect = 9.5
Identities = 12/30 (40%), Positives = 18/30 (60%)
Frame = +2
Query: 309 RFSVPEQALCFLAGANSIFAGEKLLTTANN 338
RFSV + +C G S+FA E+L+ N+
Sbjct: 869 RFSVDMRYICM*EGITSVFACERLMGKPNS 958
>BP045076
Length = 505
Score = 26.2 bits (56), Expect = 9.5
Identities = 12/30 (40%), Positives = 18/30 (60%)
Frame = -1
Query: 309 RFSVPEQALCFLAGANSIFAGEKLLTTANN 338
RFSV + +C G S+FA E+L+ N+
Sbjct: 103 RFSVDMRYICM*EGITSVFACERLMGKPNS 14
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.318 0.133 0.392
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,515,269
Number of Sequences: 28460
Number of extensions: 83434
Number of successful extensions: 428
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 427
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 427
length of query: 377
length of database: 4,897,600
effective HSP length: 92
effective length of query: 285
effective length of database: 2,279,280
effective search space: 649594800
effective search space used: 649594800
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 56 (26.2 bits)
Medicago: description of AC146331.7