BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC145767.1 - phase: 0
(191 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC10393 150 2e-37
TC12218 137 2e-33
TC18373 91 1e-19
TC10187 similar to GB|AAM91395.1|22137100|AY133565 At4g14410/dl3... 57 2e-09
BP060308 42 9e-05
TC9411 similar to UP|Q41102 (Q41102) Phaseolin G-box binding pro... 31 0.12
BI418305 30 0.35
TC19915 homologue to UP|Q39834 (Q39834) Clathrin heavy chain, pa... 29 0.45
TC12137 similar to UP|Q8L7W3 (Q8L7W3) At2g29210/F16P2.41, partia... 29 0.45
TC9974 homologue to UP|Q8H0P5 (Q8H0P5) Serine acetyltransferase ... 29 0.45
BP077029 29 0.59
BP070605 28 1.0
TC17939 weakly similar to UP|Q945P2 (Q945P2) AT5g49210/K21P3_8, ... 28 1.0
TC14213 27 1.7
TC15072 27 2.3
BP043392 27 2.3
AV415006 27 2.3
TC16038 similar to UP|Q9LXT8 (Q9LXT8) Pre-mRNA splicing factor A... 27 2.3
TC14511 similar to AAS20952 (AAS20952) Calmodulin binding protei... 27 2.9
AV407538 27 2.9
>TC10393
Length = 1063
Score = 150 bits (378), Expect = 2e-37
Identities = 78/144 (54%), Positives = 105/144 (72%), Gaps = 6/144 (4%)
Frame = +1
Query: 54 RFCDLSAVLEPGRPVRTDKPAILDDAIRVLSQLKTEAQELKESNEKLLEEIKCLKAEKNE 113
+F +L ++LEPGRP +TDK AIL DA+R+++QL+ EAQ++K++N L E+IK LK EKNE
Sbjct: 475 KFVELGSILEPGRPPKTDKAAILIDAVRMVTQLRGEAQKMKDTNMGLQEKIKELKTEKNE 654
Query: 114 LREEKLVLKADKEKIEKQLKSMPVSPAGFMPPPP------MAAYQASVNKMAVYPNYGYI 167
LR+EK LK +KE++E+QLKSM P+ FMPPP A QA NK+ + +Y +
Sbjct: 655 LRDEKQRLKTEKERLEQQLKSMNAQPS-FMPPPQALPAAFAAQGQAHGNKLVPFISYPGV 831
Query: 168 PMWHYLPQSARDTSQDHELRPPAA 191
MW +LP +ARDTSQDHELRPP A
Sbjct: 832 AMWQFLPPAARDTSQDHELRPPVA 903
>TC12218
Length = 737
Score = 137 bits (344), Expect = 2e-33
Identities = 73/140 (52%), Positives = 98/140 (69%), Gaps = 2/140 (1%)
Frame = +2
Query: 54 RFCDLSAVLEPGRPVRTDKPAILDDAIRVLSQLKTEAQELKESNEKLLEEIKCLKAEKNE 113
RF +LS++LEPGR +TDK AI+ DA+RV++QL+ EA++LKE N L E+IK LKAEKNE
Sbjct: 320 RFLELSSILEPGRQPKTDKAAIISDAVRVVTQLRNEAEKLKEMNNDLQEKIKELKAEKNE 499
Query: 114 LREEKLVLKADKEKIEKQLKSMPVSPAGFMPPPPMA--AYQASVNKMAVYPNYGYIPMWH 171
+R+EK LK DKEK+EK++K V P GF+P A A+ +K+ Y Y I MW
Sbjct: 500 IRDEKNKLKLDKEKLEKKVKLRNVQP-GFLPHADAAVKGKGAASHKLIPYIGYPGIAMWQ 676
Query: 172 YLPQSARDTSQDHELRPPAA 191
++P + DTS+DH LRPP A
Sbjct: 677 FMPSAVLDTSRDHLLRPPVA 736
>TC18373
Length = 437
Score = 90.9 bits (224), Expect = 1e-19
Identities = 44/48 (91%), Positives = 48/48 (99%)
Frame = +1
Query: 54 RFCDLSAVLEPGRPVRTDKPAILDDAIRVLSQLKTEAQELKESNEKLL 101
RFC+LS+VLEPGRPV+TDKPAILDDAIRVLSQLKTEAQELKE+NEKLL
Sbjct: 292 RFCELSSVLEPGRPVKTDKPAILDDAIRVLSQLKTEAQELKETNEKLL 435
>TC10187 similar to GB|AAM91395.1|22137100|AY133565 At4g14410/dl3245w
{Arabidopsis thaliana;}, partial (10%)
Length = 444
Score = 57.0 bits (136), Expect = 2e-09
Identities = 24/27 (88%), Positives = 24/27 (88%)
Frame = +2
Query: 165 GYIPMWHYLPQSARDTSQDHELRPPAA 191
GYIPMW YLP SARDTS DHELRPPAA
Sbjct: 2 GYIPMWQYLPSSARDTSHDHELRPPAA 82
>BP060308
Length = 354
Score = 41.6 bits (96), Expect = 9e-05
Identities = 19/32 (59%), Positives = 22/32 (68%), Gaps = 2/32 (6%)
Frame = -3
Query: 162 PNYGY--IPMWHYLPQSARDTSQDHELRPPAA 191
P GY + MW +LP +A DTSQDH LRPP A
Sbjct: 274 PIIGYPGVSMWQFLPSAAVDTSQDHVLRPPVA 179
>TC9411 similar to UP|Q41102 (Q41102) Phaseolin G-box binding protein PG2
(Fragment), partial (30%)
Length = 705
Score = 31.2 bits (69), Expect = 0.12
Identities = 19/64 (29%), Positives = 33/64 (50%)
Frame = +2
Query: 54 RFCDLSAVLEPGRPVRTDKPAILDDAIRVLSQLKTEAQELKESNEKLLEEIKCLKAEKNE 113
RF L AV+ + DK ++L DAI +++LKT+ Q + L ++ +K E +
Sbjct: 233 RFYALRAVVP--NVSKMDKASLLGDAISYITELKTKLQSSESDKTGLQKQFDAMKKELEK 406
Query: 114 LREE 117
E+
Sbjct: 407 TSEQ 418
>BI418305
Length = 437
Score = 29.6 bits (65), Expect = 0.35
Identities = 20/64 (31%), Positives = 33/64 (51%), Gaps = 3/64 (4%)
Frame = +3
Query: 90 AQELKESNEKLLEEIKCLKAEKNEL-REEKLVLKADKEKIE--KQLKSMPVSPAGFMPPP 146
A++ + E+ EE+ A++ E R+ L+ K DKEK+E + + M V P G+
Sbjct: 186 AEQTHANRERRTEEVAREYAQEQEFYRQTNLISKKDKEKVELMQAVSFMYVRPPGYNAES 365
Query: 147 PMAA 150
AA
Sbjct: 366 AKAA 377
>TC19915 homologue to UP|Q39834 (Q39834) Clathrin heavy chain, partial (5%)
Length = 447
Score = 29.3 bits (64), Expect = 0.45
Identities = 21/78 (26%), Positives = 35/78 (43%), Gaps = 5/78 (6%)
Frame = +3
Query: 97 NEKLLEEIKCLKAEKNELREEKLVLKADKEKIEKQLKSMPVSP-----AGFMPPPPMAAY 151
+E + ++I+ K K + +EEK V++ + ++P P G+ PPPPM
Sbjct: 54 DELVKDKIEAQKEVKAKEQEEKEVIQQQNMYAQLLPLALPAPPMPGMGGGYAPPPPMGGM 233
Query: 152 QASVNKMAVYPNYGYIPM 169
M P +G PM
Sbjct: 234 -----GMPPMPPFGMPPM 272
>TC12137 similar to UP|Q8L7W3 (Q8L7W3) At2g29210/F16P2.41, partial (9%)
Length = 579
Score = 29.3 bits (64), Expect = 0.45
Identities = 16/47 (34%), Positives = 26/47 (55%)
Frame = +3
Query: 88 TEAQELKESNEKLLEEIKCLKAEKNELREEKLVLKADKEKIEKQLKS 134
+E ++ KE+ K EE K K EK REE+ + ++ + +LKS
Sbjct: 33 SELEDRKETQRKKREEKKLRKEEKRRRREERRHRREERRAEKLKLKS 173
>TC9974 homologue to UP|Q8H0P5 (Q8H0P5) Serine acetyltransferase 7
(Fragment) , partial (43%)
Length = 1000
Score = 29.3 bits (64), Expect = 0.45
Identities = 18/50 (36%), Positives = 28/50 (56%), Gaps = 3/50 (6%)
Frame = +1
Query: 29 LLSVLYCVLLLHMIKGCSFIVI--VCCRFCDL-SAVLEPGRPVRTDKPAI 75
LLSVLYC+ MI C ++ +CC +L ++ PG +RT+ P +
Sbjct: 628 LLSVLYCM----MIGNCGIMLCNKICCSTQNLVLLIMSPGLVLRTEFPIV 765
>BP077029
Length = 486
Score = 28.9 bits (63), Expect = 0.59
Identities = 16/48 (33%), Positives = 28/48 (58%)
Frame = -3
Query: 83 LSQLKTEAQELKESNEKLLEEIKCLKAEKNELREEKLVLKADKEKIEK 130
LS +T + ++ EE K LKA+++ELRE+ L+ ++E +K
Sbjct: 436 LSVFQTAKVTAIKRKREVFEEGKALKAQRDELREQVPHLRDEREVAKK 293
>BP070605
Length = 393
Score = 28.1 bits (61), Expect = 1.0
Identities = 17/41 (41%), Positives = 21/41 (50%), Gaps = 3/41 (7%)
Frame = -2
Query: 139 PAGFMP-PPPMAAYQASVNKMAVYPNYGYIPMWH--YLPQS 176
P G+ P PPP Y A+ A YP GY P + Y+ QS
Sbjct: 284 PYGYPPAPPPAQGYPATGYPPAAYPPPGYPPSGYSSYVKQS 162
>TC17939 weakly similar to UP|Q945P2 (Q945P2) AT5g49210/K21P3_8, partial
(61%)
Length = 999
Score = 28.1 bits (61), Expect = 1.0
Identities = 19/48 (39%), Positives = 29/48 (59%), Gaps = 1/48 (2%)
Frame = +2
Query: 86 LKTEAQELKESNEKL-LEEIKCLKAEKNELREEKLVLKADKEKIEKQL 132
LK A++L+ K+ + E K KAEKNEL + + D+ +IEKQ+
Sbjct: 530 LKERAEKLEYWRRKVKMHEEK--KAEKNELLRRQSSMWVDEAEIEKQI 667
>TC14213
Length = 1284
Score = 27.3 bits (59), Expect = 1.7
Identities = 14/31 (45%), Positives = 16/31 (51%), Gaps = 1/31 (3%)
Frame = +1
Query: 139 PAGFMP-PPPMAAYQASVNKMAVYPNYGYIP 168
P G+ P PPP Y A+ A YP GY P
Sbjct: 868 PYGYPPAPPPAQGYPATGYPPAAYPPPGYPP 960
>TC15072
Length = 721
Score = 26.9 bits (58), Expect = 2.3
Identities = 12/32 (37%), Positives = 16/32 (49%)
Frame = +1
Query: 143 MPPPPMAAYQASVNKMAVYPNYGYIPMWHYLP 174
MPPPP Y P+YGY P ++ +P
Sbjct: 4 MPPPPQPTYP---------PSYGYPPYYNAVP 72
>BP043392
Length = 442
Score = 26.9 bits (58), Expect = 2.3
Identities = 12/32 (37%), Positives = 16/32 (49%)
Frame = -2
Query: 143 MPPPPMAAYQASVNKMAVYPNYGYIPMWHYLP 174
MPPPP Y P+YGY P ++ +P
Sbjct: 441 MPPPPQPTYP---------PSYGYPPYYNAVP 373
>AV415006
Length = 220
Score = 26.9 bits (58), Expect = 2.3
Identities = 13/28 (46%), Positives = 17/28 (60%)
Frame = -1
Query: 126 EKIEKQLKSMPVSPAGFMPPPPMAAYQA 153
E+ + LKS P S A M PPP A++A
Sbjct: 91 ERYDGTLKSPPNSVAVVMSPPPATAFRA 8
>TC16038 similar to UP|Q9LXT8 (Q9LXT8) Pre-mRNA splicing factor
ATP-dependent RNA helicase-like protein, partial (6%)
Length = 666
Score = 26.9 bits (58), Expect = 2.3
Identities = 17/51 (33%), Positives = 27/51 (52%)
Frame = +2
Query: 83 LSQLKTEAQELKESNEKLLEEIKCLKAEKNELREEKLVLKADKEKIEKQLK 133
L++L +KES+ LLE K K EK + +E LK + ++E + K
Sbjct: 68 LAELGPMFFSVKESDTSLLEHKKKQKREKTAMEQEMENLKKVQAELEPERK 220
>TC14511 similar to AAS20952 (AAS20952) Calmodulin binding protein 25,
partial (13%)
Length = 617
Score = 26.6 bits (57), Expect = 2.9
Identities = 17/43 (39%), Positives = 22/43 (50%)
Frame = +1
Query: 22 MCTITKHLLSVLYCVLLLHMIKGCSFIVIVCCRFCDLSAVLEP 64
+ T TKH L VL C L H CSF+ C + + A +EP
Sbjct: 40 LVTPTKHKLRVL-CFSLQH----CSFLFSSCLQMAENLASIEP 153
>AV407538
Length = 140
Score = 26.6 bits (57), Expect = 2.9
Identities = 12/27 (44%), Positives = 16/27 (58%)
Frame = -2
Query: 32 VLYCVLLLHMIKGCSFIVIVCCRFCDL 58
+L+ V L+ IKGC F VCC + L
Sbjct: 115 LLFWVNLMRKIKGCKFGFGVCCCYSKL 35
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.321 0.136 0.405
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,683,177
Number of Sequences: 28460
Number of extensions: 54744
Number of successful extensions: 536
Number of sequences better than 10.0: 68
Number of HSP's better than 10.0 without gapping: 528
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 534
length of query: 191
length of database: 4,897,600
effective HSP length: 85
effective length of query: 106
effective length of database: 2,478,500
effective search space: 262721000
effective search space used: 262721000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 53 (25.0 bits)
Medicago: description of AC145767.1