BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC145449.7 + phase: 0 /pseudo
(965 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV406379 103 1e-22
BP033602 93 2e-19
BP063694 87 1e-17
AU088980 83 2e-16
AV766088 78 6e-15
AV410378 71 7e-13
TC12784 similar to UP|Q850H6 (Q850H6) Gag-pol polyprotein (Fragm... 57 1e-08
AV427921 52 4e-07
AV769206 50 2e-06
BP054351 47 2e-05
BP066094 40 3e-05
AV764934 44 1e-04
TC9337 similar to UP|PME_PRUPE (Q43062) Pectinesterase PPE8B pre... 44 1e-04
BP063902 42 6e-04
TC14875 similar to UP|Q9VNX6 (Q9VNX6) CG7421-PA, partial (4%) 41 0.001
BP053302 40 0.002
AV766665 39 0.004
BP041939 39 0.004
BP061842 37 0.019
TC8093 homologue to UP|EFTU_TOBAC (P41342) Elongation factor Tu,... 37 0.019
>AV406379
Length = 226
Score = 103 bits (258), Expect = 1e-22
Identities = 45/73 (61%), Positives = 59/73 (80%)
Frame = -3
Query: 17 LYYLHSQSSNICGVSASPDMIHRRLGHPSFDKLKVLVPQLSHLKSLDCESCQLGKHVRAS 76
LYY+ S +C + SPD++H RLGHPS ++LK +VP+LSHLK+L+CESCQLGKHVRAS
Sbjct: 224 LYYMQPHLSTLCAIFESPDLLHCRLGHPSLERLKQMVPKLSHLKTLECESCQLGKHVRAS 45
Query: 77 FPSSPNKRSKSPF 89
FPSS ++ + SPF
Sbjct: 44 FPSSTSRPAVSPF 6
>BP033602
Length = 533
Score = 92.8 bits (229), Expect = 2e-19
Identities = 54/124 (43%), Positives = 73/124 (58%), Gaps = 13/124 (10%)
Frame = +3
Query: 6 KTIGT*SISQGLYYLHSQSSN-----ICGVSASP--DMI---HRRLGHPSFDKLKVLVPQ 55
K IG+ + GLYY N +CG+S + D I H RLGHP+F L+ L P
Sbjct: 156 KMIGSAKMKDGLYYFEDTFRNKAAQGLCGMSCTSVRDQIMLWHNRLGHPNFQYLRHLFPD 335
Query: 56 LS---HLKSLDCESCQLGKHVRASFPSSPNKRSKSPFDIVHSDVWGPSRVMSTLG*RYYV 112
L + SL+CESC L K+ RA + S P S+ PF ++HSDVWGPS++ + G R++V
Sbjct: 336 LFKNVNCSSLECESCVLAKNQRAPYYSQPYHASR-PFYLIHSDVWGPSKITTQFGKRWFV 512
Query: 113 TFID 116
TFID
Sbjct: 513 TFID 524
>BP063694
Length = 511
Score = 87.0 bits (214), Expect = 1e-17
Identities = 58/156 (37%), Positives = 81/156 (51%), Gaps = 11/156 (7%)
Frame = -2
Query: 4 TGKTIGT*SISQGLYYLHSQSSNICGVS-----ASPDMIHRRLGHPSFDKLKVLVPQ--- 55
TG +G QGLY L+ S + S AS ++ H RLGH +FD +K L
Sbjct: 471 TGAVLGRXRCDQGLYVLNQDSQALLTTSSPLPRASFELWHSRLGHVNFDIIKQLHKHGCL 292
Query: 56 -LSHL--KSLDCESCQLGKHVRASFPSSPNKRSKSPFDIVHSDVWGPSRVMSTLG*RYYV 112
+S + K + C SCQ+ K R F + NKR+ + D++H D+ GPS V S G Y+V
Sbjct: 291 DVSSILPKPICCTSCQMAKSKRLVFHDN-NKRASAVLDLIHCDLRGPSPVASIDGFSYFV 115
Query: 113 TFIDGFSRCTWIILLKDRSQLFGAFLTFCSEIKNQF 148
F+D FSR TW LK +S L F ++N+F
Sbjct: 114 IFVDDFSRFTWFYPLKRKSDFSDVLLRFKVFMENRF 7
>AU088980
Length = 360
Score = 83.2 bits (204), Expect = 2e-16
Identities = 41/106 (38%), Positives = 59/106 (54%)
Frame = +2
Query: 190 NGVAERKHCHLVDTTRTLLINAHAPFKFWGDAILTACYLINRMPSSVLDNEIPQSLLFPK 249
N + ERKH H+++ TR L+ +++ P W A+ A YLIN +PS +L + P L
Sbjct: 2 NSIVERKHRHILNVTRALMFHSYLPKNLWTFAVKHAAYLINXLPSPLLKGQCPYQFLNND 181
Query: 250 DPLYRVQLRVYGSTCFVHDLTPGRDKLSARAVKCVFLGYSRTQKGY 295
P + L+V+G+ C+ LT KL RA KCVFLG+ GY
Sbjct: 182 SPTL-LDLKVFGTLCYSSTLTHNXQKLDPRARKCVFLGFKTGTXGY 316
>AV766088
Length = 501
Score = 78.2 bits (191), Expect = 6e-15
Identities = 52/127 (40%), Positives = 71/127 (54%), Gaps = 6/127 (4%)
Frame = -3
Query: 14 SQGLYYLHSQSSNICGVSASPDMIHRRLGHPSFDKLKVLVP------QLSHLKSLDCESC 67
S G Y + S G++ S + H RLGHPS L+ L +L + + CESC
Sbjct: 367 SPGDLYPVTLSFPFAGIAQS--LWHSRLGHPSSSALRYLRSNKFISYELLNYSPV-CESC 197
Query: 68 QLGKHVRASFPSSPNKRSKSPFDIVHSDVWGPSRVMSTLG*RYYVTFIDGFSRCTWIILL 127
GKHVR F SS N + PFDI+HSD+W S V+S+ G R+YV F+D F+ W L
Sbjct: 196 VFGKHVRLPFVSS-NNVTVMPFDILHSDLW-TSPVLSSAGHRFYVLFLDDFTDFLWTFPL 23
Query: 128 KDRSQLF 134
++SQ+F
Sbjct: 22 SNKSQVF 2
>AV410378
Length = 358
Score = 71.2 bits (173), Expect = 7e-13
Identities = 41/117 (35%), Positives = 59/117 (50%), Gaps = 6/117 (5%)
Frame = +1
Query: 392 PKTVTTDSSDSATAPISSPVVVPPEPSNDLPIAL------HKGKRSTANPHPVYNFLSYH 445
P ++ + IS+ V PP D L K S +P+ +SYH
Sbjct: 7 PSETLSNPEEGNVQRISNRVRRPPSYLQDYHCTLAASTTVSKVPSSAGISYPLSKVISYH 186
Query: 446 RLSPSYFAFVSALSSVSIPKTVHEALSHQEWKQAMIDEMVALESNHTWELVSPSPGK 502
+L+PSY AF+ +++VS P EA+ H+ W++AM E+ ALE NHTW LV P K
Sbjct: 187 KLTPSYRAFIMNITTVSEPTRYSEAVKHECWRKAMDQEIEALERNHTWILVDKPPDK 357
>TC12784 similar to UP|Q850H6 (Q850H6) Gag-pol polyprotein (Fragment),
partial (15%)
Length = 460
Score = 57.4 bits (137), Expect = 1e-08
Identities = 46/144 (31%), Positives = 63/144 (42%), Gaps = 11/144 (7%)
Frame = +2
Query: 1 DFNTGKTIGT*SISQGLYYLHSQSSNICGV----SASPDMIHRRLGHPSFDKLKVLVPQL 56
D +T + IG S GLY L S S SP +H R GHPS L+ L P L
Sbjct: 38 DLSTPRIIGRGRESDGLYLLDEGVSKASTAYRTSSVSPFQLHCRFGHPSLGVLRKLCPPL 217
Query: 57 SHLKSLDCESCQLG-KHVRASFPSSPNKRSKSPFDIVHSDVWGPS------RVMSTLG*R 109
+ + CESC + R+ + NKR+ S F IVH G R+ +L
Sbjct: 218 ESVSNFSCESCHVC*TSXRSVYLPRLNKRAMSLFHIVHL*CMGSMPCNF*IRLRVSLSHL 397
Query: 110 YYVTFIDGFSRCTWIILLKDRSQL 133
+ + R W+ L+K RS+L
Sbjct: 398 WMIIL-----RAPWLYLMKSRSEL 454
>AV427921
Length = 284
Score = 52.0 bits (123), Expect = 4e-07
Identities = 24/72 (33%), Positives = 45/72 (62%)
Frame = +1
Query: 180 QSSCPHTPQQNGVAERKHCHLVDTTRTLLINAHAPFKFWGDAILTACYLINRMPSSVLDN 239
Q + +TPQQNGV+ERK+ +++ R+LL + P F +A++ + +++NR P+ V+ N
Sbjct: 4 Q*TAAYTPQQNGVSERKNRTILNMVRSLLTMSGLPKSFLPEAVMWSLHILNRSPTLVVQN 183
Query: 240 EIPQSLLFPKDP 251
+P+ + P
Sbjct: 184 MMPEEAWSGRQP 219
>AV769206
Length = 536
Score = 49.7 bits (117), Expect = 2e-06
Identities = 39/117 (33%), Positives = 52/117 (44%), Gaps = 3/117 (2%)
Frame = -3
Query: 257 LRVYGSTCFVHDLTPGRDKLSARAVKCVFLGYSRTQKGYRCYSPSTRRFYISADVTFFED 316
LR Y ST KLS + +CVF+GYS KGY+C S R YIS DV F E
Sbjct: 531 LRPYNST-----------KLSLHSKECVFIGYSINHKGYKCLDQS-GRIYISKDVLFHEH 388
Query: 317 T---PFFASPTTTSSTTDVTDSQVIPTPLFHPIFEPPVSTQSSPQLQSNPEFRRYGN 370
P TTSST + F P+ P+ + ++P ++ F G+
Sbjct: 387 RFPYPSLFPDETTSST----------SAEFFPLSTIPIVSHTTPPALASSGFSSLGS 247
>BP054351
Length = 431
Score = 46.6 bits (109), Expect = 2e-05
Identities = 30/77 (38%), Positives = 40/77 (50%), Gaps = 13/77 (16%)
Frame = -1
Query: 4 TGKTIGT*SISQGLYYLHSQSSN-----ICGVSASP--DMI---HRRLGHPSFDKLKVLV 53
+GK I + + GLYY N +CG+S + D I H RLGHP+F L+ L
Sbjct: 236 SGKMIASAKMKDGLYYFEDTFRNKAAQGLCGMSCTSVRDQIMLWHNRLGHPNFQYLRHLF 57
Query: 54 PQL---SHLKSLDCESC 67
P L + SL+CESC
Sbjct: 56 PDLFKNVNCSSLECESC 6
>BP066094
Length = 532
Score = 39.7 bits (91), Expect(2) = 3e-05
Identities = 17/45 (37%), Positives = 30/45 (65%)
Frame = +2
Query: 549 AKMTSVRLFIAMTAMKR*PLFQLDIKNAFLHGDLEEEIYMEQPSG 593
+K ++RL I+ + L Q+++K+AFL+G + EE+Y+ QP G
Sbjct: 86 SKTEAIRLLISFSVNHNIILHQMNVKSAFLNGYISEEVYVHQPPG 220
Score = 25.4 bits (54), Expect(2) = 3e-05
Identities = 10/18 (55%), Positives = 14/18 (77%)
Frame = +3
Query: 533 YTQVYGQDYNDTFSPVAK 550
Y+Q G DY +TF+PVA+
Sbjct: 39 YSQQ*GIDYTETFAPVAR 92
>AV764934
Length = 406
Score = 43.9 bits (102), Expect = 1e-04
Identities = 27/72 (37%), Positives = 40/72 (55%), Gaps = 5/72 (6%)
Frame = -2
Query: 38 HRRLGHPSFDKLKVLVPQ--LSHLKSLDCE---SCQLGKHVRASFPSSPNKRSKSPFDIV 92
H RLGHP D LK ++ +S+ E +C LGK R PS + + PF+++
Sbjct: 210 HSRLGHPHLDNLKRVLSSCNVSYSPKDTVEFRTACCLGKAHR--LPSQMSTTTYLPFELI 37
Query: 93 HSDVWGPSRVMS 104
+SD+WGP+ V S
Sbjct: 36 YSDLWGPAPVCS 1
>TC9337 similar to UP|PME_PRUPE (Q43062) Pectinesterase PPE8B precursor
(Pectin methylesterase) (PE) , partial (54%)
Length = 1145
Score = 43.9 bits (102), Expect = 1e-04
Identities = 20/34 (58%), Positives = 24/34 (69%)
Frame = +3
Query: 64 CESCQLGKHVRASFPSSPNKRSKSPFDIVHSDVW 97
C+SC LGKHVR F SS + PFDI+HSD+W
Sbjct: 1029 CDSCVLGKHVRLPFSSSETITLR-PFDILHSDLW 1127
>BP063902
Length = 494
Score = 41.6 bits (96), Expect = 6e-04
Identities = 29/73 (39%), Positives = 41/73 (55%), Gaps = 6/73 (8%)
Frame = -3
Query: 14 SQGLYYLHSQSSNICGVSASPDMIHRRLGHPSFDKL------KVLVPQLSHLKSLDCESC 67
S G Y ++SS G+++S + H RLGHP+ L K++ + S S+ C+SC
Sbjct: 243 SLGDLYPVTRSSPFAGLASS--VWHHRLGHPASSALNHLRNNKLIFCEPSRSSSV-CDSC 73
Query: 68 QLGKHVRASFPSS 80
LGKHVR F SS
Sbjct: 72 VLGKHVRLPFSSS 34
>TC14875 similar to UP|Q9VNX6 (Q9VNX6) CG7421-PA, partial (4%)
Length = 1240
Score = 40.8 bits (94), Expect = 0.001
Identities = 42/155 (27%), Positives = 58/155 (37%), Gaps = 36/155 (23%)
Frame = +1
Query: 317 TPFFASPTTTSSTTDVTDSQV-------IPTPLFHPIFEPPVSTQS----SPQLQSNPEF 365
T FAS T+S +T + S +P P P P ST + SP ++P
Sbjct: 31 TTSFASSLTSSDSTKMAASNA*WAQTPPLPAPTI*PPSNPKTSTSTPTPASPPASTSP-- 204
Query: 366 RRYGNIYERRHVEAPETSPIDS------SDSAPKTVTTDSSDSATA-------------- 405
+ P TSP S S SAP T T ++ S T+
Sbjct: 205 -----------LTPPTTSPSSSTSTAAPSASAPLTTTPTTATSTTSPRRQTPSSSPSTTA 351
Query: 406 -----PISSPVVVPPEPSNDLPIALHKGKRSTANP 435
P SP P +PS+ LP + +G R+T P
Sbjct: 352 SPRSTPSPSPTTTPGKPSSGLPASTTRGSRTTPIP 456
>BP053302
Length = 432
Score = 40.0 bits (92), Expect = 0.002
Identities = 29/78 (37%), Positives = 39/78 (49%), Gaps = 16/78 (20%)
Frame = -1
Query: 1 DFNTGKTIGT*SISQGLYYLHSQSSN-------------ICGVSASPDMI---HRRLGHP 44
D N+GK IG S GLYYL S++ ++ + D + H RLGHP
Sbjct: 243 DLNSGKMIGRAKESGGLYYLDLGSASPLPPKTISSCFESFFVLNNNDDHVMLWHLRLGHP 64
Query: 45 SFDKLKVLVPQLSHLKSL 62
SF LK L P+L + K+L
Sbjct: 63 SFHYLKPLFPKLFYNKNL 10
>AV766665
Length = 601
Score = 38.9 bits (89), Expect = 0.004
Identities = 22/52 (42%), Positives = 29/52 (55%)
Frame = +2
Query: 821 YRWVFRCRLGRRCE**TVYF*LLYFCGWKSHFMEEQKANSRCSIKYRSRVSS 872
Y WV+ CRL ** ++ +LY WK ME+ A C I++RSR SS
Sbjct: 440 YGWVYICRLFGVYC**VIHHGVLYVFEWKFSDMEK*AAKYNCQIEWRSRTSS 595
>BP041939
Length = 392
Score = 38.9 bits (89), Expect = 0.004
Identities = 26/87 (29%), Positives = 40/87 (45%), Gaps = 1/87 (1%)
Frame = +3
Query: 274 DKLSARAVKCVFLGYSRTQKGYRCYSPSTRRFYISADVTFFEDTPFFA-SPTTTSSTTDV 332
D++ ++ ++ G + C+SP R+ +SADVTFFED F SP++ + +
Sbjct: 36 DQVGSKVIEVCVPGLLSYLERVPCFSPDLGRYLVSADVTFFEDCSFSGWSPSSFPTYSRR 215
Query: 333 TDSQVIPTPLFHPIFEPPVSTQSSPQL 359
S PP TQS P L
Sbjct: 216 PKSSA-----------PPSDTQSLPSL 263
>BP061842
Length = 318
Score = 36.6 bits (83), Expect = 0.019
Identities = 13/29 (44%), Positives = 22/29 (75%)
Frame = -3
Query: 88 PFDIVHSDVWGPSRVMSTLG*RYYVTFID 116
P ++V D+WGP+ +ST G +YY++F+D
Sbjct: 91 PLELVPCDLWGPAPCVSTNGFKYYLSFVD 5
>TC8093 homologue to UP|EFTU_TOBAC (P41342) Elongation factor Tu,
chloroplast precursor (EF-Tu), partial (58%)
Length = 1146
Score = 36.6 bits (83), Expect = 0.019
Identities = 35/129 (27%), Positives = 54/129 (41%), Gaps = 1/129 (0%)
Frame = +2
Query: 322 SPTTTSSTTDVTDSQVIPTPLFHPIFEPPVSTQSSPQLQSNPEFRRYGNIYERRHVEAPE 381
S T T +T + + P P + PP S S QS+ RR P
Sbjct: 131 SSTHTLLLLPLTLTPLPPASSSDPNYPPPTSPPPSILPQSSSTRRRNNPPPPSTAAPPPS 310
Query: 382 TS-PIDSSDSAPKTVTTDSSDSATAPISSPVVVPPEPSNDLPIALHKGKRSTANPHPVYN 440
S P+ +S SA ++T S+ SAT+ + P PP P LP + +T + P+ +
Sbjct: 311 PSAPLAASSSARNPMST-SAPSATSTTAKPPSPPPSPWPSLPSETAPPRNTTKSTPPLKS 487
Query: 441 FLSYHRLSP 449
L+ +P
Sbjct: 488 ALAVSPSTP 514
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.339 0.146 0.489
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,980,132
Number of Sequences: 28460
Number of extensions: 307034
Number of successful extensions: 2781
Number of sequences better than 10.0: 119
Number of HSP's better than 10.0 without gapping: 2631
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2739
length of query: 965
length of database: 4,897,600
effective HSP length: 99
effective length of query: 866
effective length of database: 2,080,060
effective search space: 1801331960
effective search space used: 1801331960
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.8 bits)
S2: 60 (27.7 bits)
Medicago: description of AC145449.7