BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC145372.5 - phase: 0
(369 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC19879 similar to UP|O82657 (O82657) Pyruvate dehydrogenase kin... 315 6e-87
TC9135 homologue to UP|Q9SBJ1 (Q9SBJ1) Pyruvate dehydrogenase ki... 218 1e-57
TC11331 similar to UP|O82657 (O82657) Pyruvate dehydrogenase kin... 128 2e-30
BP043144 29 1.1
TC11811 similar to PIR|D96538|D96538 cytosolic tRNA-Ala syntheta... 28 1.9
TC16088 similar to UP|Q8PX92 (Q8PX92) Chemotaxis protein CheW, p... 28 2.4
TC20225 27 4.1
TC11299 27 4.1
TC17163 27 5.4
BP085705 27 7.1
>TC19879 similar to UP|O82657 (O82657) Pyruvate dehydrogenase kinase ,
partial (47%)
Length = 614
Score = 315 bits (808), Expect = 6e-87
Identities = 150/183 (81%), Positives = 170/183 (91%)
Frame = +2
Query: 1 MAAKKAIETFSKSLIEEVHKWGCFKQTGVSLRYMMDFGSKPTSKNLLISAQFLHKELAIR 60
MAAKKA ETFSK+L+E+VH+WGC KQTGVSLRYMM+FGS PT KNLLISAQFLHKELAIR
Sbjct: 65 MAAKKACETFSKALMEDVHRWGCMKQTGVSLRYMMEFGSTPTEKNLLISAQFLHKELAIR 244
Query: 61 IARRAVELERLPCDLSQKPAVLKVRDWYLDSFRDIRSFSEIKNMNDEKEFTEVIKAIKVR 120
IARRA+EL+ LP LS+KPAV+KVRDWY+DSFRD+RS+ +IKNM DEK+FTE+IKAIKVR
Sbjct: 245 IARRAIELDSLPYGLSKKPAVIKVRDWYVDSFRDLRSYPDIKNMTDEKDFTEMIKAIKVR 424
Query: 121 HNNVVPTMALGVQQLKKDVDPKIINQDFVEIHQFLDRFYMSRIGIRMLIGQHVELHSPNP 180
HNNVVPTMALGVQQLKK +DPKI+ +D +EIHQFLDRFYMSRIGIRMLIGQHVELH+PNP
Sbjct: 425 HNNVVPTMALGVQQLKKGMDPKIVYEDLIEIHQFLDRFYMSRIGIRMLIGQHVELHNPNP 604
Query: 181 PPH 183
PH
Sbjct: 605 APH 613
>TC9135 homologue to UP|Q9SBJ1 (Q9SBJ1) Pyruvate dehydrogenase kinase ,
partial (31%)
Length = 538
Score = 218 bits (556), Expect = 1e-57
Identities = 104/117 (88%), Positives = 111/117 (93%)
Frame = +1
Query: 253 ERYMNSDKDPPPIRIIVADGLEDVTIKISDEGGGIPRSGLRKIFTYLYSTARNPLDEHAD 312
ER+++SD PP+RIIVADGLEDVTIK+SDEGGGIPRSGL KIFTYLYSTARNPLDEH D
Sbjct: 1 ERFIDSDNVAPPVRIIVADGLEDVTIKVSDEGGGIPRSGLPKIFTYLYSTARNPLDEHED 180
Query: 313 LGVGDNVTMAGYGYGLPISRLYARYFGGDLQIISMENYGTDAYLHLSRLGDSQEPLP 369
LG+GDNVTMAGYGYG+PISRLYARYFGGDLQIISME YGTDAYLHLSRLGDSQEPLP
Sbjct: 181 LGLGDNVTMAGYGYGIPISRLYARYFGGDLQIISMEGYGTDAYLHLSRLGDSQEPLP 351
>TC11331 similar to UP|O82657 (O82657) Pyruvate dehydrogenase kinase ,
partial (21%)
Length = 384
Score = 128 bits (321), Expect = 2e-30
Identities = 62/77 (80%), Positives = 70/77 (90%)
Frame = +2
Query: 1 MAAKKAIETFSKSLIEEVHKWGCFKQTGVSLRYMMDFGSKPTSKNLLISAQFLHKELAIR 60
MAAKKA ETFSK+L+E+VH+WGC KQTGVSLRYMM+FGS PT KNLLISAQFLHKELAIR
Sbjct: 152 MAAKKACETFSKALMEDVHRWGCMKQTGVSLRYMMEFGSTPTEKNLLISAQFLHKELAIR 331
Query: 61 IARRAVELERLPCDLSQ 77
IARRA+EL+ LP LS+
Sbjct: 332 IARRAIELDSLPYGLSK 382
>BP043144
Length = 439
Score = 29.3 bits (64), Expect = 1.1
Identities = 17/47 (36%), Positives = 24/47 (50%)
Frame = +2
Query: 249 RAVEERYMNSDKDPPPIRIIVADGLEDVTIKISDEGGGIPRSGLRKI 295
R VEE NS D ++ V + +K+ D+GGGI R GL +
Sbjct: 227 RVVEELVFNS-LDARATKVSVFVNVGSCYLKVVDDGGGITRDGLELV 364
>TC11811 similar to PIR|D96538|D96538 cytosolic tRNA-Ala synthetase
[imported] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (18%)
Length = 680
Score = 28.5 bits (62), Expect = 1.9
Identities = 11/16 (68%), Positives = 12/16 (74%)
Frame = +2
Query: 326 YGLPISRLYARYFGGD 341
Y LP R+YA YFGGD
Sbjct: 524 YKLPSDRIYATYFGGD 571
>TC16088 similar to UP|Q8PX92 (Q8PX92) Chemotaxis protein CheW, partial (8%)
Length = 769
Score = 28.1 bits (61), Expect = 2.4
Identities = 11/23 (47%), Positives = 13/23 (55%)
Frame = +2
Query: 172 HVELHSPNPPPHVVGYIHTRMSP 194
H +H P+PP HV Y H SP
Sbjct: 263 HDHVHQPSPPQHVNQYSHYPTSP 331
>TC20225
Length = 591
Score = 27.3 bits (59), Expect = 4.1
Identities = 13/28 (46%), Positives = 15/28 (53%)
Frame = +1
Query: 191 RMSPVEVARNAVDDARSICSRQYGSAPD 218
R P VA + DA CSR+ G APD
Sbjct: 385 RKGPTLVAPRVLRDAHGGCSRKEGQAPD 468
>TC11299
Length = 498
Score = 27.3 bits (59), Expect = 4.1
Identities = 25/112 (22%), Positives = 47/112 (41%), Gaps = 3/112 (2%)
Frame = +1
Query: 68 LERLPCDLSQKPAVLKVRDWYLDSFRDIRSFSEIKNMNDEKE---FTEVIKAIKVRHNNV 124
L LP + + AVL R +L FS ++ + F++++ ++ + N
Sbjct: 127 LSFLPTENAVATAVLSKRWTHLWRSVSALDFSSVRMYEPDDHRFFFSDIVYSVLLFRNAA 306
Query: 125 VPTMALGVQQLKKDVDPKIINQDFVEIHQFLDRFYMSRIGIRMLIGQHVELH 176
P L+ D D + N D IH++++ R+G +H+ LH
Sbjct: 307 TPIKKFS---LELDDDVAVANLDIANIHKWVNFVTQCRVGGI----EHLRLH 441
>TC17163
Length = 514
Score = 26.9 bits (58), Expect = 5.4
Identities = 10/25 (40%), Positives = 16/25 (64%)
Frame = -3
Query: 158 FYMSRIGIRMLIGQHVELHSPNPPP 182
FY+ R+ I +L+ +E +PN PP
Sbjct: 338 FYLHRVKITILMSHKLEKKNPNSPP 264
>BP085705
Length = 358
Score = 26.6 bits (57), Expect = 7.1
Identities = 15/41 (36%), Positives = 19/41 (45%), Gaps = 7/41 (17%)
Frame = +2
Query: 152 HQFLDRFYMSRIGIRMLIGQ-------HVELHSPNPPPHVV 185
HQ + S IG+R Q H H+PNPPP +V
Sbjct: 173 HQLIGVHSYSLIGVRSYSSQGPGQYSHHHRHHNPNPPPLMV 295
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.321 0.140 0.412
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,759,165
Number of Sequences: 28460
Number of extensions: 75586
Number of successful extensions: 416
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 412
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 414
length of query: 369
length of database: 4,897,600
effective HSP length: 92
effective length of query: 277
effective length of database: 2,279,280
effective search space: 631360560
effective search space used: 631360560
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 56 (26.2 bits)
Medicago: description of AC145372.5