BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC145331.12 - phase: 0
(419 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC18061 104 3e-23
AV410973 56 1e-08
AU252214 33 0.11
TC11317 similar to UP|Q40363 (Q40363) NuM1 protein, partial (12%) 32 0.20
TC17253 similar to UP|Q8SKU2 (Q8SKU2) Tic62 protein precursor, p... 32 0.26
BP042908 31 0.44
TC10461 homologue to UP|BAD11346 (BAD11346) BRI1-KD interacting ... 29 1.3
TC9596 similar to UP|AAR24722 (AAR24722) At5g49400, partial (66%) 29 1.3
TC10633 29 1.7
TC18568 29 1.7
TC13322 similar to UP|Q9ZS13 (Q9ZS13) RNA helicase (Fragment), p... 28 2.2
TC15860 similar to UP|Q94KS1 (Q94KS1) RNA helicase SDE3, partial... 28 2.8
TC13672 28 2.8
TC15410 28 2.8
TC16373 similar to UP|O64690 (O64690) At2g34570 protein, partial... 28 3.7
TC9941 27 4.8
AV780912 27 4.8
TC11967 homologue to UP|AAS09978 (AAS09978) MYB transcription fa... 27 4.8
TC11151 similar to UP|BAA82348 (BAA82348) Starch branching enzym... 27 4.8
TC9883 homologue to UP|AOX2_SOYBN (Q41266) Alternative oxidase 2... 27 4.8
>TC18061
Length = 543
Score = 104 bits (259), Expect = 3e-23
Identities = 58/115 (50%), Positives = 76/115 (65%), Gaps = 1/115 (0%)
Frame = -1
Query: 1 MDPVNSLPADGLDDVHQNGVHDEPSNSGEDAVSNDLDPHVTVNTETFVPDGNSENINQLE 60
MD N DGL VHQNG +E N ED V ++ D V +ET +P+G+ N NQL+
Sbjct: 357 MDHSNLDHEDGLQAVHQNGGREEQPNFVEDGVVSNGDSVVAEISETVIPNGSFGNFNQLD 178
Query: 61 STATGNSAMKEI-EGSNDNVDGSNLTVSKEKEVKIKVSTEQSRAQKGPVKNKNAK 114
STAT N +M EI EG +D + G+N+++SKE+EV+I TEQ+R KGP KNKNAK
Sbjct: 177 STATDNLSMTEIKEGLDDTIYGNNVSISKEEEVEIVDETEQARGPKGPAKNKNAK 13
>AV410973
Length = 270
Score = 56.2 bits (134), Expect = 1e-08
Identities = 30/46 (65%), Positives = 34/46 (73%)
Frame = +3
Query: 197 LRKGESSSLRLRKRFRQRRRRRVACKQNPRRVKKPRLKSLGRA*LL 242
LR+ +SS+L LRKRFRQRRRRR CK RR KK RL+ LGR * L
Sbjct: 132 LREEKSSTLSLRKRFRQRRRRRQTCKPKARRPKKLRLRCLGRV*RL 269
Score = 29.6 bits (65), Expect = 0.97
Identities = 21/64 (32%), Positives = 28/64 (42%), Gaps = 16/64 (25%)
Frame = +2
Query: 176 LKKGPLDKVQGEGESSL----------------YPFSLRKGESSSLRLRKRFRQRRRRRV 219
L KGP+DKVQ E ESS Y FS + GE + RK F + ++
Sbjct: 2 LNKGPIDKVQREAESSSGDTEDAKPRRLGTLPDYGFSFKCGERA--ERRKEFYTKLEEKI 175
Query: 220 ACKQ 223
K+
Sbjct: 176 QAKE 187
>AU252214
Length = 350
Score = 32.7 bits (73), Expect = 0.11
Identities = 23/77 (29%), Positives = 35/77 (44%)
Frame = +2
Query: 151 RPRQPIKNRSSNDRQSQLSKKKPKSLKKGPLDKVQGEGESSLYPFSLRKGESSSLRLRKR 210
R R+P+ R +R+ + + K + + G KVQG P R+ R R+R
Sbjct: 98 RRRKPVLVRHQRNRRRRTKRLKRRRISSGKRRKVQGHA-----PLKRRRKRP---RRRRR 253
Query: 211 FRQRRRRRVACKQNPRR 227
R+ RRRV + RR
Sbjct: 254 QRRNERRRVG*RSRRRR 304
>TC11317 similar to UP|Q40363 (Q40363) NuM1 protein, partial (12%)
Length = 547
Score = 32.0 bits (71), Expect = 0.20
Identities = 31/147 (21%), Positives = 60/147 (40%), Gaps = 3/147 (2%)
Frame = +1
Query: 48 VPDGNSENINQLESTATGNSAMKEIEGSNDNVDGSNLTVSKEKEVKIKVSTEQSRAQKGP 107
+P + ++ ++E+ A +A K + + + V K+ K + E + QK
Sbjct: 82 MPKSSKKSATKVEAAAPVAAAPKSAKKAKRAAEDE---VEKQVSAKKQKIAEVAAKQKKE 252
Query: 108 VKNKNAKVGSSSGVNASLVKNSKIGKDKQASPAVSNGTSALDSRPRQPIKNRS---SNDR 164
K + K SSS ++S K +V NGT+ P + S S+D
Sbjct: 253 TKLQKVKKESSSDDDSSSEDEKPAPKVAPLKTSVKNGTTPAKKAKPAPSSSSSDEDSSDE 432
Query: 165 QSQLSKKKPKSLKKGPLDKVQGEGESS 191
+ ++ KKP + ++ + +SS
Sbjct: 433 EEEVIAKKPTKVVVPKKEESSSDEDSS 513
>TC17253 similar to UP|Q8SKU2 (Q8SKU2) Tic62 protein precursor, partial (9%)
Length = 793
Score = 31.6 bits (70), Expect = 0.26
Identities = 25/108 (23%), Positives = 42/108 (38%)
Frame = +2
Query: 39 HVTVNTETFVPDGNSENINQLESTATGNSAMKEIEGSNDNVDGSNLTVSKEKEVKIKVST 98
+V +TE N ++EST + +A E+E + DNV S S V
Sbjct: 44 NVAASTEVESTGDNVSASTEVESTGSNVAAPTEVESTGDNVAASTEVESTGDNVAASTEV 223
Query: 99 EQSRAQKGPVKNKNAKVGSSSGVNASLVKNSKIGKDKQASPAVSNGTS 146
E + + V + V AS S +G + +P+ + +S
Sbjct: 224 ESTGSN---VAAPTEVESTGDNVAASTTVESTVGNVDEKNPSPDSNSS 358
Score = 31.2 bits (69), Expect = 0.33
Identities = 36/142 (25%), Positives = 55/142 (38%), Gaps = 8/142 (5%)
Frame = +2
Query: 40 VTVNTETFVPDGNSENINQLESTATGNSAMKEIEGSNDNV--------DGSNLTVSKEKE 91
+ +TE N ++EST SA E+E + NV G N+ S E E
Sbjct: 8 LAASTEVESTGDNVAASTEVESTGDNVSASTEVESTGSNVAAPTEVESTGDNVAASTEVE 187
Query: 92 VKIKVSTEQSRAQKGPVKNKNAKVGSSSGVNASLVKNSKIGKDKQASPAVSNGTSALDSR 151
ST + A V++ + V + + V ++ G + AS V + +D +
Sbjct: 188 -----STGDNVAASTEVESTGSNVAAPTEVEST-------GDNVAASTTVESTVGNVDEK 331
Query: 152 PRQPIKNRSSNDRQSQLSKKKP 173
P N SSN S KP
Sbjct: 332 NPSPDSN-SSNSPYPVYSDFKP 394
Score = 31.2 bits (69), Expect = 0.33
Identities = 33/141 (23%), Positives = 54/141 (37%), Gaps = 10/141 (7%)
Frame = +2
Query: 39 HVTVNTETFVPDGNSENINQLESTATGNSAMKEIEGSNDNVDGSNLTVSKEKEVKIKVST 98
+V+ +TE N ++EST +A E+E + DNV S S V
Sbjct: 83 NVSASTEVESTGSNVAAPTEVESTGDNVAASTEVESTGDNVAASTEVESTGSNVAAPTEV 262
Query: 99 EQS----------RAQKGPVKNKNAKVGSSSGVNASLVKNSKIGKDKQASPAVSNGTSAL 148
E + + G V KN S+S N+ S SP N +++
Sbjct: 263 ESTGDNVAASTTVESTVGNVDEKNPSPDSNSS-NSPYPVYSDFKPPTSPSPNAPNTSTSK 439
Query: 149 DSRPRQPIKNRSSNDRQSQLS 169
+ P + +S++ +QLS
Sbjct: 440 SAVDVVPEMDAASSNGPAQLS 502
>BP042908
Length = 327
Score = 30.8 bits (68), Expect = 0.44
Identities = 18/33 (54%), Positives = 23/33 (69%), Gaps = 2/33 (6%)
Frame = +2
Query: 206 RLRKRFRQRRRRRVAC-KQNPRRVK-KPRLKSL 236
R R RFR RRRRR+ C ++ PRR + +PRL L
Sbjct: 77 RRRLRFRTRRRRRLRCTRRAPRRRRLRPRLPRL 175
>TC10461 homologue to UP|BAD11346 (BAD11346) BRI1-KD interacting protein 118
(Fragment), partial (7%)
Length = 636
Score = 29.3 bits (64), Expect = 1.3
Identities = 20/40 (50%), Positives = 26/40 (65%)
Frame = +2
Query: 226 RRVKKPRLKSLGRA*LLKQLHYQLSIRSLLLLRWS*RRYQ 265
R+ K+ R ++ GRA K L Q+SIR+ LLR S RRYQ
Sbjct: 20 RKTKRQRSRN*GRAWHSKLLQCQVSIRN-HLLRLSLRRYQ 136
>TC9596 similar to UP|AAR24722 (AAR24722) At5g49400, partial (66%)
Length = 1196
Score = 29.3 bits (64), Expect = 1.3
Identities = 30/141 (21%), Positives = 54/141 (38%), Gaps = 1/141 (0%)
Frame = +2
Query: 83 NLTVSKEKEVK-IKVSTEQSRAQKGPVKNKNAKVGSSSGVNASLVKNSKIGKDKQASPAV 141
N++VS + E + E++++ K K+ S + V ++ G
Sbjct: 545 NVSVSYDLENNDVDDKEEKAKSSSKKAKRKHRSDSDSGSDSEDSVFETESGSSSVTGSDY 724
Query: 142 SNGTSALDSRPRQPIKNRSSNDRQSQLSKKKPKSLKKGPLDKVQGEGESSLYPFSLRKGE 201
S+G+S+ S + S + + + KKK + KKG K S S +
Sbjct: 725 SSGSSSDSS-------SSDSEEERRRRRKKKTQKKKKGRRHKKYSSSSESSDSESASDSD 883
Query: 202 SSSLRLRKRFRQRRRRRVACK 222
S R++ R RR + K
Sbjct: 884 SDDKSSRRKRRHSRRH*IRLK 946
>TC10633
Length = 593
Score = 28.9 bits (63), Expect = 1.7
Identities = 25/92 (27%), Positives = 42/92 (45%), Gaps = 8/92 (8%)
Frame = -2
Query: 133 KDKQASPAVSNGTSALDSRPRQPIKNRSSNDRQSQLSKKKPKSLKKGP-LDKVQGEGESS 191
+++ PA + +R R+ + +RS +S L + + LD+ ++
Sbjct: 352 EERNLPPAPYRQAAEAQARRRRKLHHRSRFHHRSPLRALVQRETESNSDLDR*SRRYPAT 173
Query: 192 LYPFSL-------RKGESSSLRLRKRFRQRRR 216
L P L R+G SSLR+R+R R RRR
Sbjct: 172 LVPPLLSVRRGHRRRGSWSSLRIRRRCRLRRR 77
>TC18568
Length = 719
Score = 28.9 bits (63), Expect = 1.7
Identities = 32/110 (29%), Positives = 51/110 (46%), Gaps = 8/110 (7%)
Frame = +3
Query: 75 SNDNVDGSNLTVSKEK-------EVKIKVSTEQSRAQKGPVKNKNAKVGSSS-GVNASLV 126
S++N +GSN V KEK E ++K + A K +N+ KV +S+ ++AS
Sbjct: 126 SSNNNNGSNNEVKKEKKEEEDEDEKEVKSRIQHPLADKS--QNQMLKVVTSTMAMHASSN 299
Query: 127 KNSKIGKDKQASPAVSNGTSALDSRPRQPIKNRSSNDRQSQLSKKKPKSL 176
KNS G + + S+ T A S I N + + K K K++
Sbjct: 300 KNSNQGNETTQTSQNSSQTPAKQSYSYSGI-NMKGRELEDPEVKAKMKAM 446
>TC13322 similar to UP|Q9ZS13 (Q9ZS13) RNA helicase (Fragment), partial (7%)
Length = 528
Score = 28.5 bits (62), Expect = 2.2
Identities = 18/73 (24%), Positives = 33/73 (44%)
Frame = +2
Query: 151 RPRQPIKNRSSNDRQSQLSKKKPKSLKKGPLDKVQGEGESSLYPFSLRKGESSSLRLRKR 210
+PR PI+ + Q Q SK++ +K + +G +S + + E R++
Sbjct: 113 QPRTPIREEEEKEEQRQASKRRENQSQK----EARGTRQSKRHRIGEEEKEEEG-EARQQ 277
Query: 211 FRQRRRRRVACKQ 223
R+ RRR K+
Sbjct: 278 VRRERRRNQRSKR 316
>TC15860 similar to UP|Q94KS1 (Q94KS1) RNA helicase SDE3, partial (5%)
Length = 774
Score = 28.1 bits (61), Expect = 2.8
Identities = 16/34 (47%), Positives = 22/34 (64%), Gaps = 3/34 (8%)
Frame = +2
Query: 5 NSLPAD--GLDDVHQNGVHDEPSNSGE-DAVSND 35
N+LP++ G D NG D+PSN+GE D SN+
Sbjct: 218 NALPSNNGGWDQPSNNGEWDQPSNNGEWDQPSNN 319
>TC13672
Length = 598
Score = 28.1 bits (61), Expect = 2.8
Identities = 16/59 (27%), Positives = 27/59 (45%)
Frame = +2
Query: 24 PSNSGEDAVSNDLDPHVTVNTETFVPDGNSENINQLESTATGNSAMKEIEGSNDNVDGS 82
PSN N + H T + P ++EN+++ E + G S + G+N +V S
Sbjct: 2 PSNVQNPNTKNQAEKHNVPPTSSAPPKSSTENVSKPE-RSVGTSNPSRVFGANSHVSSS 175
>TC15410
Length = 610
Score = 28.1 bits (61), Expect = 2.8
Identities = 36/150 (24%), Positives = 62/150 (41%), Gaps = 8/150 (5%)
Frame = +3
Query: 21 HDEPSNSGEDAVSNDLDPHVTVNTETFVPDGNSENINQLESTATGNSAMKEIEGSNDNVD 80
HD+ S+ G+D + NDL P E + S+ ++ ++ + + ++E +DN
Sbjct: 15 HDDNSSEGDDDIGNDLSPEEMKVAERAIA-VVSDTLSVVKELIRSITGLIKLEKPSDN-- 185
Query: 81 GSNLTVSKEKEVKIKVSTEQSRAQKGPVKNKNAKVGSSSGVNASLVKNSKIGKDKQASPA 140
S S EK +++ + + G ++ S +NA+L K Q A
Sbjct: 186 -SEFVNSLEKLLQLCQELGKQIDEIGACLYPPQEIPS---INAALEKIHSTIDVVQVEVA 353
Query: 141 VSNGTS--------ALDSRPRQPIKNRSSN 162
GTS AL S RQ + SS+
Sbjct: 354 ALQGTSDVFLEACNALRSSLRQLVSELSSS 443
>TC16373 similar to UP|O64690 (O64690) At2g34570 protein, partial (9%)
Length = 981
Score = 27.7 bits (60), Expect = 3.7
Identities = 19/80 (23%), Positives = 38/80 (46%), Gaps = 2/80 (2%)
Frame = +1
Query: 143 NGTSALDSRPRQPIKNRSSNDRQSQLSKKKPKSLKKGPLD--KVQGEGESSLYPFSLRKG 200
+G A++ + + I +R+ D + + K+ ++ PL K + ++ KG
Sbjct: 94 SGDQAINVQAVRSITSRNKTDIKDKPQFKRKRAKGPNPLSCKKKKSHENPNIGSMKEMKG 273
Query: 201 ESSSLRLRKRFRQRRRRRVA 220
+ + R RKR R R+R+ A
Sbjct: 274 DDTVRRSRKRTRSRKRQMPA 333
>TC9941
Length = 557
Score = 27.3 bits (59), Expect = 4.8
Identities = 13/32 (40%), Positives = 18/32 (55%)
Frame = -2
Query: 49 PDGNSENINQLESTATGNSAMKEIEGSNDNVD 80
P G SEN+ +T +G+ +M EIE S D
Sbjct: 211 PRGFSENLTHARATVSGSGSMGEIEVSEVGFD 116
>AV780912
Length = 210
Score = 27.3 bits (59), Expect = 4.8
Identities = 16/55 (29%), Positives = 26/55 (47%)
Frame = +3
Query: 121 VNASLVKNSKIGKDKQASPAVSNGTSALDSRPRQPIKNRSSNDRQSQLSKKKPKS 175
V S ++K+GK + P S A S+P Q + +N QS +K + K+
Sbjct: 36 VTTSTKVDNKLGKGSRNLPKTSKNPQA*ASKPYQLPLKQPTNSNQSNATKLETKT 200
>TC11967 homologue to UP|AAS09978 (AAS09978) MYB transcription factor,
partial (32%)
Length = 664
Score = 27.3 bits (59), Expect = 4.8
Identities = 16/34 (47%), Positives = 22/34 (64%), Gaps = 1/34 (2%)
Frame = +1
Query: 331 LLKGPIQQLHLH-LRQQRKILAYPKVQGKRKLRL 363
LL GP ++L H L++ K+L +PK QG LRL
Sbjct: 415 LLLGPREKLLTHTLKKLPKMLLHPKPQGHCNLRL 516
>TC11151 similar to UP|BAA82348 (BAA82348) Starch branching enzyme ,
partial (11%)
Length = 567
Score = 27.3 bits (59), Expect = 4.8
Identities = 17/50 (34%), Positives = 23/50 (46%), Gaps = 6/50 (12%)
Frame = +2
Query: 21 HDEPSNSGEDAVSN------DLDPHVTVNTETFVPDGNSENINQLESTAT 64
HD S S A S+ DLD ++ E PD +E++ LE AT
Sbjct: 371 HDSDSTSSAIAESDKVLVPQDLDNSASLTDEVETPDITTEDVQNLEDLAT 520
>TC9883 homologue to UP|AOX2_SOYBN (Q41266) Alternative oxidase 2,
mitochondrial precursor , partial (34%)
Length = 665
Score = 27.3 bits (59), Expect = 4.8
Identities = 12/22 (54%), Positives = 16/22 (72%)
Frame = +2
Query: 206 RLRKRFRQRRRRRVACKQNPRR 227
R R+R ++RRRRR C+Q RR
Sbjct: 254 RRRRRRKRRRRRRRVCEQRLRR 319
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.324 0.137 0.398
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,183,265
Number of Sequences: 28460
Number of extensions: 79598
Number of successful extensions: 816
Number of sequences better than 10.0: 44
Number of HSP's better than 10.0 without gapping: 775
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 801
length of query: 419
length of database: 4,897,600
effective HSP length: 93
effective length of query: 326
effective length of database: 2,250,820
effective search space: 733767320
effective search space used: 733767320
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 56 (26.2 bits)
Medicago: description of AC145331.12