Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC145331.1 - phase: 0 
         (520 letters)

Database: LJGI 
           28,460 sequences; 14,692,800 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC17762                                                                34  0.066
BP034485                                                               33  0.15
TC19934                                                                30  0.73
AV428640                                                               30  1.3
AV416115                                                               29  1.6
TC9635                                                                 28  2.8
TC9489 homologue to UP|Q9SEI5 (Q9SEI5) 26S proteasome AAA-ATPase...    28  3.6
TC7811 homologue to PIR|T04368|T04368 plasma membrane intrinsic ...    28  4.8
TC8080                                                                 27  6.2
TC8030                                                                 27  6.2
AV421390                                                               27  6.2
TC10924 homologue to UP|Q8H9D4 (Q8H9D4) 26S proteasome AAA-ATPas...    27  8.1
BP056402                                                               27  8.1
BP036526                                                               27  8.1
TC18482                                                                27  8.1
TC15365 homologue to GB|AAP78936.1|32306505|BT009668 At5g19990 {...    27  8.1
TC16349 homologue to UP|PRS6_SOLTU (P54778) 26S protease regulat...    27  8.1
TC9200 similar to UP|Q8LLE6 (Q8LLE6) RNA-binding protein AKIP1, ...    27  8.1
TC14544                                                                27  8.1

>TC17762 
          Length = 463

 Score = 33.9 bits (76), Expect = 0.066
 Identities = 13/39 (33%), Positives = 22/39 (56%)
 Frame = -3

Query: 36  WWKRLNNKITERWTNSRKRPENVDPGVGKWVSYLKREKK 74
           WW+R+  K T RW ++R+R          WV+  K+E++
Sbjct: 185 WWRRVRVKETWRWQHARERKRLAVVAAWPWVAAAKKERR 69


>BP034485 
          Length = 485

 Score = 32.7 bits (73), Expect = 0.15
 Identities = 14/45 (31%), Positives = 26/45 (57%), Gaps = 2/45 (4%)
 Frame = +2

Query: 11  WEKKRENERRRILMDEVEKQQNDKDWWKR--LNNKITERWTNSRK 53
           WE+++E  RRR   D  ++Q+ +++ W+R     +  ERW   R+
Sbjct: 128 WEEEQEGRRRRRR*DSKQEQEQEQEQWRRKQQRERRKERWRRRRE 262


>TC19934 
          Length = 384

 Score = 30.4 bits (67), Expect = 0.73
 Identities = 13/36 (36%), Positives = 18/36 (49%)
 Frame = +3

Query: 134 EHMHKTWKKQMEEKPLQSGISSWIMNLPFDTKAKEW 169
           E  H  WK+   +K L+    SW+ N P  T+ K W
Sbjct: 165 ELFHPAWKRHHCKKVLKKIFQSWLTN*PLCTQHKTW 272


>AV428640 
          Length = 368

 Score = 29.6 bits (65), Expect = 1.3
 Identities = 13/24 (54%), Positives = 16/24 (66%)
 Frame = +2

Query: 75  LDSLVGRRPTAPPAPKGLYIYGNV 98
           L S + +RP  PP PKGL I GN+
Sbjct: 95  LASRIRKRPPYPPGPKGLPIIGNM 166


>AV416115 
          Length = 430

 Score = 29.3 bits (64), Expect = 1.6
 Identities = 12/30 (40%), Positives = 17/30 (56%)
 Frame = +1

Query: 118 KHRRRYHFHEAMLRINEHMHKTWKKQMEEK 147
           +HR R+H H      N H  + WKK+ EE+
Sbjct: 52  RHRNRHHHH------NHHERRRWKKRPEEE 123


>TC9635 
          Length = 585

 Score = 28.5 bits (62), Expect = 2.8
 Identities = 14/36 (38%), Positives = 20/36 (54%)
 Frame = +3

Query: 367 NYHTVFISDIPMMSMRIRDKARRFITLIDELYNHHS 402
           NYH VF   +P   MR+R   +  I  + ++Y HHS
Sbjct: 42  NYHRVFKGIMPSPGMRVRLSNKLQIQGMPKVYWHHS 149


>TC9489 homologue to UP|Q9SEI5 (Q9SEI5) 26S proteasome AAA-ATPase subunit
           RPT2a, partial (69%)
          Length = 916

 Score = 28.1 bits (61), Expect = 3.6
 Identities = 11/25 (44%), Positives = 17/25 (68%)
 Frame = +1

Query: 89  PKGLYIYGNVGSGKTMLMDMFYSAT 113
           PKG+ +YG  G+GKT+L     ++T
Sbjct: 484 PKGVILYGEPGTGKTLLAKAVANST 558


>TC7811 homologue to PIR|T04368|T04368 plasma membrane intrinsic protein
           BPW2 - barley {Hordeum vulgare;} , partial (15%)
          Length = 464

 Score = 27.7 bits (60), Expect = 4.8
 Identities = 14/43 (32%), Positives = 19/43 (43%)
 Frame = +1

Query: 363 AVAENYHTVFISDIPMMSMRIRDKARRFITLIDELYNHHSCLC 405
           A+A  YH V I  +P  S  +        T + E +N   CLC
Sbjct: 46  ALAAIYHVVVIRALPFQSQ*LESNGSLIRTTVWENHNPSHCLC 174


>TC8080 
          Length = 1432

 Score = 27.3 bits (59), Expect = 6.2
 Identities = 17/58 (29%), Positives = 30/58 (51%)
 Frame = -1

Query: 130 LRINEHMHKTWKKQMEEKPLQSGISSWIMNLPFDTKAKEWLAAEERYKKEVQMKHILP 187
           L+I+ H+H     Q EE+ +   +  WI+  PF  +   +L A +  K++  + H LP
Sbjct: 580 LQISRHLH-----QEEEETI---L*DWILENPFSCRQIYFLTARKTVKQKEFLVHQLP 431


>TC8030 
          Length = 991

 Score = 27.3 bits (59), Expect = 6.2
 Identities = 17/53 (32%), Positives = 22/53 (41%)
 Frame = -1

Query: 15  RENERRRILMDEVEKQQNDKDWWKRLNNKITERWTNSRKRPENVDPGVGKWVS 67
           RENER R              WW R+  K T RW ++R+R          WV+
Sbjct: 247 RENERGR--------GDGGCPWWWRVPVKDTWRWQHARERKRLAVVAAWPWVT 113


>AV421390 
          Length = 409

 Score = 27.3 bits (59), Expect = 6.2
 Identities = 15/40 (37%), Positives = 21/40 (52%)
 Frame = +2

Query: 66  VSYLKREKKLDSLVGRRPTAPPAPKGLYIYGNVGSGKTML 105
           V YL+   K   L G+       PKG+ + G  G+GKT+L
Sbjct: 119 VEYLRNPAKFTRLGGK------LPKGILLTGAPGTGKTLL 220


>TC10924 homologue to UP|Q8H9D4 (Q8H9D4) 26S proteasome AAA-ATPase subunit
           RPT4a, partial (49%)
          Length = 648

 Score = 26.9 bits (58), Expect = 8.1
 Identities = 10/17 (58%), Positives = 14/17 (81%)
 Frame = +2

Query: 89  PKGLYIYGNVGSGKTML 105
           PKG+ +YG  G+GKT+L
Sbjct: 560 PKGVLLYGPPGTGKTLL 610


>BP056402 
          Length = 475

 Score = 26.9 bits (58), Expect = 8.1
 Identities = 11/39 (28%), Positives = 21/39 (53%)
 Frame = +3

Query: 41  NNKITERWTNSRKRPENVDPGVGKWVSYLKREKKLDSLV 79
           +N+ T RW  + K  +N++PG      Y   E+ +D ++
Sbjct: 189 SNEATSRWRPTNKTTDNLNPGT---ACYSAAEESVDKII 296


>BP036526 
          Length = 531

 Score = 26.9 bits (58), Expect = 8.1
 Identities = 24/96 (25%), Positives = 42/96 (43%), Gaps = 7/96 (7%)
 Frame = +2

Query: 208 CFDEIQTVDVFAIVALSGILSRLLSSGTIIVATSNRAPKD------LNEA-NMVPEFFQN 260
           CF E++ +   A    SG+ + + S  T IV  +   P         NE  ++ P+  + 
Sbjct: 47  CFAEVKQLSFSAKTLYSGLHALVPSLSTAIVDKNLGFPVFSAIDALFNEGLSLPPQKARG 226

Query: 261 LLSNLEEHCEKVLVGSEIDYRRFIAQRSENRVNYLW 296
           LLS L     K++  +E D  RF    + ++  + W
Sbjct: 227 LLSGLLPRLVKLIQDTENDVLRFETPATMDKDRFFW 334


>TC18482 
          Length = 530

 Score = 26.9 bits (58), Expect = 8.1
 Identities = 15/53 (28%), Positives = 29/53 (54%), Gaps = 4/53 (7%)
 Frame = -3

Query: 262 LSNLEEHCEKVLVGSEIDYRRF----IAQRSENRVNYLWPIERETINKFEKKW 310
           +S+++E  E  +  +E ++ R     + ++ E RV  LW +    I +FEK+W
Sbjct: 207 VSHVQEELEGGMAETETEWWRIEEVGL*RKGEERVG-LW*VSGSEIGRFEKRW 52


>TC15365 homologue to GB|AAP78936.1|32306505|BT009668 At5g19990 {Arabidopsis
           thaliana;}, partial (87%)
          Length = 1385

 Score = 26.9 bits (58), Expect = 8.1
 Identities = 10/17 (58%), Positives = 14/17 (81%)
 Frame = +3

Query: 89  PKGLYIYGNVGSGKTML 105
           PKG+ +YG  G+GKT+L
Sbjct: 429 PKGVLLYGPPGTGKTLL 479


>TC16349 homologue to UP|PRS6_SOLTU (P54778) 26S protease regulatory subunit
           6B homolog, partial (65%)
          Length = 901

 Score = 26.9 bits (58), Expect = 8.1
 Identities = 10/17 (58%), Positives = 14/17 (81%)
 Frame = +3

Query: 89  PKGLYIYGNVGSGKTML 105
           P+G+ +YG  G+GKTML
Sbjct: 600 PRGVLLYGPPGTGKTML 650


>TC9200 similar to UP|Q8LLE6 (Q8LLE6) RNA-binding protein AKIP1, partial
           (31%)
          Length = 727

 Score = 26.9 bits (58), Expect = 8.1
 Identities = 11/45 (24%), Positives = 23/45 (50%)
 Frame = +2

Query: 11  WEKKRENERRRILMDEVEKQQNDKDWWKRLNNKITERWTNSRKRP 55
           W +K++  +R+  + + +K++  K  WKR   + T   + S   P
Sbjct: 230 WRRKKKRRKRKRRVKKKKKKKKKKRKWKRKRRRKTMNRSRSFSNP 364


>TC14544 
          Length = 1160

 Score = 26.9 bits (58), Expect = 8.1
 Identities = 9/22 (40%), Positives = 12/22 (53%)
 Frame = +2

Query: 44  ITERWTNSRKRPENVDPGVGKW 65
           I + WT S    E +DP  G+W
Sbjct: 173 IADNWTRSAHWAETLDPATGRW 238


  Database: LJGI
    Posted date:  Jul 30, 2004 11:16 AM
  Number of letters in database: 14,692,800
  Number of sequences in database:  28,460
  
Lambda     K      H
   0.318    0.135    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,925,601
Number of Sequences: 28460
Number of extensions: 117788
Number of successful extensions: 751
Number of sequences better than 10.0: 39
Number of HSP's better than 10.0 without gapping: 745
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 751
length of query: 520
length of database: 4,897,600
effective HSP length: 94
effective length of query: 426
effective length of database: 2,222,360
effective search space: 946725360
effective search space used: 946725360
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 57 (26.6 bits)


Medicago: description of AC145331.1